Expression of legumain correlates with prognosis and metastasis in gastric carcinoma
Legumain, a novel asparaginyl endopeptidase, has been observed to be highly expressed in several types of tumors, which may play a vital role in carcinogenesis. However, there is no study investigating the relationship among Legumain expression, clinicopathologic, biological variables and patient pr...
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| Veröffentlicht in: | PloS one 2013-09, Vol.8 (9), p.e73090 |
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| creator | Guo, Pengtao Zhu, Zhi Sun, Zhe Wang, Zhenning Zheng, Xinyu Xu, Huimian |
| description | Legumain, a novel asparaginyl endopeptidase, has been observed to be highly expressed in several types of tumors, which may play a vital role in carcinogenesis. However, there is no study investigating the relationship among Legumain expression, clinicopathologic, biological variables and patient prognosis in gastric carcinoma.
In this study, a tissue microarray (TMA) containing 282 samples of primary gastric cancer was assessed for Legumain expression by immunohistochemistry. The TMA included 98 lymph node metastasis samples. The protein expression levels of Legumain were evaluated by Western blot analysis.
Cytoplasmic immunoreactivity of Legumain was over-expressed in gastric cancer compared with paired normal gastric mucosa. Increased Legumain levels were significantly correlated with clinical stage, presence of distant metastasis. Legumain was significantly over-expressed in primary gastric cancer with metastasis than without metastasis. Patients with Legumain-positive localized tumors had lower 5-year overall survival (OS) than those with Legumain-negative tumors. Multivariate survival analysis showed that Legumain was an independent prognostic marker for OS (HR 1.459, 95% CI 1.251-1.703, P = 0.007).
Legumain expression could serve as a prognostic biomarker in patients at risk of developing metastasis or recurrence with gastric carcinoma. |
| doi_str_mv | 10.1371/journal.pone.0073090 |
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In this study, a tissue microarray (TMA) containing 282 samples of primary gastric cancer was assessed for Legumain expression by immunohistochemistry. The TMA included 98 lymph node metastasis samples. The protein expression levels of Legumain were evaluated by Western blot analysis.
Cytoplasmic immunoreactivity of Legumain was over-expressed in gastric cancer compared with paired normal gastric mucosa. Increased Legumain levels were significantly correlated with clinical stage, presence of distant metastasis. Legumain was significantly over-expressed in primary gastric cancer with metastasis than without metastasis. Patients with Legumain-positive localized tumors had lower 5-year overall survival (OS) than those with Legumain-negative tumors. Multivariate survival analysis showed that Legumain was an independent prognostic marker for OS (HR 1.459, 95% CI 1.251-1.703, P = 0.007).
Legumain expression could serve as a prognostic biomarker in patients at risk of developing metastasis or recurrence with gastric carcinoma.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0073090</identifier><identifier>PMID: 24023813</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Angiogenesis ; Biomarkers ; Breast cancer ; Cancer ; Carcinogenesis ; Carcinogens ; Cell growth ; Committees ; Cysteine Endopeptidases - genetics ; Cysteine Endopeptidases - metabolism ; Development and progression ; Endopeptidase ; Endopeptidases ; Extracellular matrix ; Female ; Gastric cancer ; Gastric mucosa ; Gastric Mucosa - metabolism ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genetic aspects ; Humans ; Immunohistochemistry ; Kinases ; Legumain ; Lymph nodes ; Lymphatic Metastasis ; Lymphatic system ; Male ; Medical prognosis ; Metastases ; Metastasis ; Middle Aged ; Oncology ; Patients ; Physiological aspects ; Prognosis ; Prostate ; Stomach cancer ; Stomach Neoplasms - diagnosis ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology ; Surgery ; Survival ; Survival Analysis ; Tumors ; Young Adult</subject><ispartof>PloS one, 2013-09, Vol.8 (9), p.e73090</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Guo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Guo et al 2013 Guo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-1adccf2d94afff1cac9499f5f0bf00a02352c6ab7c7d84256d7aa787532811373</citedby><cites>FETCH-LOGICAL-c692t-1adccf2d94afff1cac9499f5f0bf00a02352c6ab7c7d84256d7aa787532811373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759407/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3759407/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24023813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Pengtao</creatorcontrib><creatorcontrib>Zhu, Zhi</creatorcontrib><creatorcontrib>Sun, Zhe</creatorcontrib><creatorcontrib>Wang, Zhenning</creatorcontrib><creatorcontrib>Zheng, Xinyu</creatorcontrib><creatorcontrib>Xu, Huimian</creatorcontrib><title>Expression of legumain correlates with prognosis and metastasis in gastric carcinoma</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Legumain, a novel asparaginyl endopeptidase, has been observed to be highly expressed in several types of tumors, which may play a vital role in carcinogenesis. However, there is no study investigating the relationship among Legumain expression, clinicopathologic, biological variables and patient prognosis in gastric carcinoma.
In this study, a tissue microarray (TMA) containing 282 samples of primary gastric cancer was assessed for Legumain expression by immunohistochemistry. The TMA included 98 lymph node metastasis samples. The protein expression levels of Legumain were evaluated by Western blot analysis.
Cytoplasmic immunoreactivity of Legumain was over-expressed in gastric cancer compared with paired normal gastric mucosa. Increased Legumain levels were significantly correlated with clinical stage, presence of distant metastasis. Legumain was significantly over-expressed in primary gastric cancer with metastasis than without metastasis. Patients with Legumain-positive localized tumors had lower 5-year overall survival (OS) than those with Legumain-negative tumors. Multivariate survival analysis showed that Legumain was an independent prognostic marker for OS (HR 1.459, 95% CI 1.251-1.703, P = 0.007).
Legumain expression could serve as a prognostic biomarker in patients at risk of developing metastasis or recurrence with gastric carcinoma.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Angiogenesis</subject><subject>Biomarkers</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Cell growth</subject><subject>Committees</subject><subject>Cysteine Endopeptidases - genetics</subject><subject>Cysteine Endopeptidases - metabolism</subject><subject>Development and progression</subject><subject>Endopeptidase</subject><subject>Endopeptidases</subject><subject>Extracellular matrix</subject><subject>Female</subject><subject>Gastric cancer</subject><subject>Gastric mucosa</subject><subject>Gastric Mucosa - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genetic aspects</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kinases</subject><subject>Legumain</subject><subject>Lymph nodes</subject><subject>Lymphatic Metastasis</subject><subject>Lymphatic system</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>Oncology</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Prognosis</subject><subject>Prostate</subject><subject>Stomach cancer</subject><subject>Stomach Neoplasms - diagnosis</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><subject>Surgery</subject><subject>Survival</subject><subject>Survival Analysis</subject><subject>Tumors</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEUhgdRbK3-A9EBQfBi13xOJjeFUqouFApavQ1nM8lslpnJmmS0_nuz7rTsgIIkkK_nvDm8vEXxEqMlpgK_3_oxDNAtd34wS4QERRI9Kk6xpGRREUQfH-1PimcxbhHitK6qp8UJYYjQGtPT4vbqbhdMjM4PpbdlZ9qxBzeU2odgOkgmlj9d2pS74NvBRxdLGJqyNwlinvmY2Tbvg9OlhqDd4Ht4Xjyx0EXzYlrPiq8frm4vPy2ubz6uLi-uF7qSJC0wNFpb0kgG1lqsQUsmpeUWrS1CkFvkRFewFlo0NSO8agSAqAWnpMbZA3pWvD7o7jof1WRIVJgRyTCuhMzE6kA0HrZqF1wP4Zfy4NSfCx9aBSE53RllMGiOuBb1GpgADDybyvSaAZUV5zZrnU-_jeveNNoMKUA3E52_DG6jWv9DUcElQ_t230wCwX8fTUz_aHmiWshducH6LKZ7F7W6YKJmjCCEMrX8C5VHY3qncySsy_ezgnezgswkc5daGGNUqy-f_5-9-TZn3x6xGwNd2kTfjSknKs5BdgB18DEGYx-cw0jtE33vhtonWk2JzmWvjl1_KLqPMP0NvDvxhg</recordid><startdate>20130902</startdate><enddate>20130902</enddate><creator>Guo, Pengtao</creator><creator>Zhu, Zhi</creator><creator>Sun, Zhe</creator><creator>Wang, Zhenning</creator><creator>Zheng, Xinyu</creator><creator>Xu, Huimian</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130902</creationdate><title>Expression of legumain correlates with prognosis and metastasis in gastric carcinoma</title><author>Guo, Pengtao ; Zhu, Zhi ; Sun, Zhe ; Wang, Zhenning ; Zheng, Xinyu ; Xu, Huimian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-1adccf2d94afff1cac9499f5f0bf00a02352c6ab7c7d84256d7aa787532811373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Angiogenesis</topic><topic>Biomarkers</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Carcinogenesis</topic><topic>Carcinogens</topic><topic>Cell growth</topic><topic>Committees</topic><topic>Cysteine Endopeptidases - genetics</topic><topic>Cysteine Endopeptidases - metabolism</topic><topic>Development and progression</topic><topic>Endopeptidase</topic><topic>Endopeptidases</topic><topic>Extracellular matrix</topic><topic>Female</topic><topic>Gastric cancer</topic><topic>Gastric mucosa</topic><topic>Gastric Mucosa - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genetic aspects</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kinases</topic><topic>Legumain</topic><topic>Lymph nodes</topic><topic>Lymphatic Metastasis</topic><topic>Lymphatic system</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>Oncology</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Prognosis</topic><topic>Prostate</topic><topic>Stomach cancer</topic><topic>Stomach Neoplasms - diagnosis</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><topic>Surgery</topic><topic>Survival</topic><topic>Survival Analysis</topic><topic>Tumors</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Pengtao</creatorcontrib><creatorcontrib>Zhu, Zhi</creatorcontrib><creatorcontrib>Sun, Zhe</creatorcontrib><creatorcontrib>Wang, Zhenning</creatorcontrib><creatorcontrib>Zheng, Xinyu</creatorcontrib><creatorcontrib>Xu, Huimian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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However, there is no study investigating the relationship among Legumain expression, clinicopathologic, biological variables and patient prognosis in gastric carcinoma.
In this study, a tissue microarray (TMA) containing 282 samples of primary gastric cancer was assessed for Legumain expression by immunohistochemistry. The TMA included 98 lymph node metastasis samples. The protein expression levels of Legumain were evaluated by Western blot analysis.
Cytoplasmic immunoreactivity of Legumain was over-expressed in gastric cancer compared with paired normal gastric mucosa. Increased Legumain levels were significantly correlated with clinical stage, presence of distant metastasis. Legumain was significantly over-expressed in primary gastric cancer with metastasis than without metastasis. Patients with Legumain-positive localized tumors had lower 5-year overall survival (OS) than those with Legumain-negative tumors. Multivariate survival analysis showed that Legumain was an independent prognostic marker for OS (HR 1.459, 95% CI 1.251-1.703, P = 0.007).
Legumain expression could serve as a prognostic biomarker in patients at risk of developing metastasis or recurrence with gastric carcinoma.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24023813</pmid><doi>10.1371/journal.pone.0073090</doi><tpages>e73090</tpages><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Aged Aged, 80 and over Angiogenesis Biomarkers Breast cancer Cancer Carcinogenesis Carcinogens Cell growth Committees Cysteine Endopeptidases - genetics Cysteine Endopeptidases - metabolism Development and progression Endopeptidase Endopeptidases Extracellular matrix Female Gastric cancer Gastric mucosa Gastric Mucosa - metabolism Gene expression Gene Expression Regulation, Neoplastic Genetic aspects Humans Immunohistochemistry Kinases Legumain Lymph nodes Lymphatic Metastasis Lymphatic system Male Medical prognosis Metastases Metastasis Middle Aged Oncology Patients Physiological aspects Prognosis Prostate Stomach cancer Stomach Neoplasms - diagnosis Stomach Neoplasms - genetics Stomach Neoplasms - metabolism Stomach Neoplasms - pathology Surgery Survival Survival Analysis Tumors Young Adult |
| title | Expression of legumain correlates with prognosis and metastasis in gastric carcinoma |
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