Increase in sialylation and branching in the mouse serum N-glycome correlates with inflammation and ovarian tumour progression
Ovarian cancer is the most lethal gynaecological cancer and is often diagnosed in late stage, often as the result of the unavailability of sufficiently sensitive biomarkers for early detection, tumour progression and tumour-associated inflammation. Glycosylation is the most common posttranslational...
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| creator | Saldova, Radka Piccard, Helene Pérez-Garay, Marta Harvey, David J Struwe, Weston B Galligan, Marie C Berghmans, Nele Madden, Stephen F Peracaula, Rosa Opdenakker, Ghislain Rudd, Pauline M |
| description | Ovarian cancer is the most lethal gynaecological cancer and is often diagnosed in late stage, often as the result of the unavailability of sufficiently sensitive biomarkers for early detection, tumour progression and tumour-associated inflammation. Glycosylation is the most common posttranslational modification of proteins; it is altered in cancer and therefore is a potential source of biomarkers. We investigated the quantitative and qualitative effects of anti-inflammatory (acetylsalicylic acid) and pro-inflammatory (thioglycolate and chlorite-oxidized oxyamylose) drugs on glycosylation in mouse cancer serum. A significant increase in sialylation and branching of glycans in mice treated with an inflammation-inducing compound was observed. Moreover, the increases in sialylation correlated with increased tumour sizes. Increases in sialylation and branching were consistent with increased expression of sialyltransferases and the branching enzyme MGAT5. Because the sialyltransferases are highly conserved among species, the described changes in the ovarian cancer mouse model are relevant to humans and serum N-glycome analysis for monitoring disease treatment and progression might be a useful biomarker. |
| doi_str_mv | 10.1371/journal.pone.0071159 |
| format | Article |
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Glycosylation is the most common posttranslational modification of proteins; it is altered in cancer and therefore is a potential source of biomarkers. We investigated the quantitative and qualitative effects of anti-inflammatory (acetylsalicylic acid) and pro-inflammatory (thioglycolate and chlorite-oxidized oxyamylose) drugs on glycosylation in mouse cancer serum. A significant increase in sialylation and branching of glycans in mice treated with an inflammation-inducing compound was observed. Moreover, the increases in sialylation correlated with increased tumour sizes. Increases in sialylation and branching were consistent with increased expression of sialyltransferases and the branching enzyme MGAT5. Because the sialyltransferases are highly conserved among species, the described changes in the ovarian cancer mouse model are relevant to humans and serum N-glycome analysis for monitoring disease treatment and progression might be a useful biomarker.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0071159</identifier><identifier>PMID: 24023608</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetylsalicylic acid ; Amylose - administration & dosage ; Amylose - analogs & derivatives ; Amylose - pharmacology ; Animals ; Biology ; Biomarkers ; Cancer ; Cancer therapies ; Carbohydrates ; Cell Line, Tumor ; Chemokines ; Chromatography ; Chromatography, High Pressure Liquid ; Diabetes ; Disease Models, Animal ; Disease Progression ; Drugs ; Female ; Glycoproteins - blood ; Glycoproteins - chemistry ; Glycosylation ; Gynecology ; Humans ; Inflammation ; Inflammation - blood ; Inflammation - complications ; Inflammation - pathology ; Laboratories ; Mass spectrometry ; Medical research ; Medical treatment ; Medicine ; Mice ; Molecular Weight ; N-Acetylneuraminic Acid - metabolism ; Neuraminic Acids - metabolism ; Ovarian cancer ; Ovarian carcinoma ; Ovarian Neoplasms - blood ; Ovarian Neoplasms - complications ; Ovarian Neoplasms - immunology ; Ovarian Neoplasms - pathology ; Polysaccharides ; Proteins ; Proteomics ; Scientific imaging ; Sialyltransferases - metabolism ; Studies ; Thioglycolates - administration & dosage ; Thioglycolates - pharmacology ; Tumor Burden - drug effects ; Tumors</subject><ispartof>PloS one, 2013-08, Vol.8 (8), p.e71159-e71159</ispartof><rights>2013 Fahey (Saldova) et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Glycosylation is the most common posttranslational modification of proteins; it is altered in cancer and therefore is a potential source of biomarkers. We investigated the quantitative and qualitative effects of anti-inflammatory (acetylsalicylic acid) and pro-inflammatory (thioglycolate and chlorite-oxidized oxyamylose) drugs on glycosylation in mouse cancer serum. A significant increase in sialylation and branching of glycans in mice treated with an inflammation-inducing compound was observed. Moreover, the increases in sialylation correlated with increased tumour sizes. Increases in sialylation and branching were consistent with increased expression of sialyltransferases and the branching enzyme MGAT5. Because the sialyltransferases are highly conserved among species, the described changes in the ovarian cancer mouse model are relevant to humans and serum N-glycome analysis for monitoring disease treatment and progression might be a useful biomarker.</description><subject>Acetylsalicylic acid</subject><subject>Amylose - administration & dosage</subject><subject>Amylose - analogs & derivatives</subject><subject>Amylose - pharmacology</subject><subject>Animals</subject><subject>Biology</subject><subject>Biomarkers</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Carbohydrates</subject><subject>Cell Line, Tumor</subject><subject>Chemokines</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Diabetes</subject><subject>Disease Models, Animal</subject><subject>Disease Progression</subject><subject>Drugs</subject><subject>Female</subject><subject>Glycoproteins - blood</subject><subject>Glycoproteins - chemistry</subject><subject>Glycosylation</subject><subject>Gynecology</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Inflammation - blood</subject><subject>Inflammation - complications</subject><subject>Inflammation - pathology</subject><subject>Laboratories</subject><subject>Mass spectrometry</subject><subject>Medical research</subject><subject>Medical treatment</subject><subject>Medicine</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>N-Acetylneuraminic Acid - metabolism</subject><subject>Neuraminic Acids - metabolism</subject><subject>Ovarian cancer</subject><subject>Ovarian carcinoma</subject><subject>Ovarian Neoplasms - blood</subject><subject>Ovarian Neoplasms - complications</subject><subject>Ovarian Neoplasms - immunology</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Polysaccharides</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Scientific imaging</subject><subject>Sialyltransferases - metabolism</subject><subject>Studies</subject><subject>Thioglycolates - administration & dosage</subject><subject>Thioglycolates - pharmacology</subject><subject>Tumor Burden - 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Glycosylation is the most common posttranslational modification of proteins; it is altered in cancer and therefore is a potential source of biomarkers. We investigated the quantitative and qualitative effects of anti-inflammatory (acetylsalicylic acid) and pro-inflammatory (thioglycolate and chlorite-oxidized oxyamylose) drugs on glycosylation in mouse cancer serum. A significant increase in sialylation and branching of glycans in mice treated with an inflammation-inducing compound was observed. Moreover, the increases in sialylation correlated with increased tumour sizes. Increases in sialylation and branching were consistent with increased expression of sialyltransferases and the branching enzyme MGAT5. Because the sialyltransferases are highly conserved among species, the described changes in the ovarian cancer mouse model are relevant to humans and serum N-glycome analysis for monitoring disease treatment and progression might be a useful biomarker.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24023608</pmid><doi>10.1371/journal.pone.0071159</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-08, Vol.8 (8), p.e71159-e71159 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acetylsalicylic acid Amylose - administration & dosage Amylose - analogs & derivatives Amylose - pharmacology Animals Biology Biomarkers Cancer Cancer therapies Carbohydrates Cell Line, Tumor Chemokines Chromatography Chromatography, High Pressure Liquid Diabetes Disease Models, Animal Disease Progression Drugs Female Glycoproteins - blood Glycoproteins - chemistry Glycosylation Gynecology Humans Inflammation Inflammation - blood Inflammation - complications Inflammation - pathology Laboratories Mass spectrometry Medical research Medical treatment Medicine Mice Molecular Weight N-Acetylneuraminic Acid - metabolism Neuraminic Acids - metabolism Ovarian cancer Ovarian carcinoma Ovarian Neoplasms - blood Ovarian Neoplasms - complications Ovarian Neoplasms - immunology Ovarian Neoplasms - pathology Polysaccharides Proteins Proteomics Scientific imaging Sialyltransferases - metabolism Studies Thioglycolates - administration & dosage Thioglycolates - pharmacology Tumor Burden - drug effects Tumors |
| title | Increase in sialylation and branching in the mouse serum N-glycome correlates with inflammation and ovarian tumour progression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T16%3A12%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Increase%20in%20sialylation%20and%20branching%20in%20the%20mouse%20serum%20N-glycome%20correlates%20with%20inflammation%20and%20ovarian%20tumour%20progression&rft.jtitle=PloS%20one&rft.au=Saldova,%20Radka&rft.date=2013-08-30&rft.volume=8&rft.issue=8&rft.spage=e71159&rft.epage=e71159&rft.pages=e71159-e71159&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0071159&rft_dat=%3Cproquest_plos_%3E3059398611%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1428934845&rft_id=info:pmid/24023608&rft_doaj_id=oai_doaj_org_article_eb35c5ff15784526a2bde178641a4006&rfr_iscdi=true |