Antioxidant capacity of the leaf extract obtained from Arrabidaea chica cultivated in Southern Brazil
Arrabidaea chica leaf extract has been used by people as an anti-inflammatory and astringent agent as well as a remedy for intestinal colic, diarrhea, leucorrhea, anemia, and leukemia. A. chica is known to be a good producer of phenolics. Therefore, in the present study, we investigated its antioxid...
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| Veröffentlicht in: | PloS one 2013-08, Vol.8 (8), p.e72733-e72733 |
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| creator | Siraichi, Jackeline Tiemy Guinoza Felipe, Daniele Fernanda Brambilla, Lara Zampar Serra Gatto, Melissa Junqueira Terra, Vânia Aparecida Cecchini, Alessandra Lourenco Cortez, Lucia Elaine Ranieri Rodrigues-Filho, Edson Cortez, Diógenes Aparício Garcia |
| description | Arrabidaea chica leaf extract has been used by people as an anti-inflammatory and astringent agent as well as a remedy for intestinal colic, diarrhea, leucorrhea, anemia, and leukemia. A. chica is known to be a good producer of phenolics. Therefore, in the present study, we investigated its antioxidant activity. The phenolic composition of A. chica leaves was studied by liquid chromatography coupled to diode array detection (LC-DAD) and liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), and isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein, luteolin, and apigenin were identified. The extract from leaves of A. chica was tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, β-carotene bleaching test, and total reactive antioxidant potential (TRAP) method. The crude extract quenched DPPH free radicals in a dose-dependent manner, and the IC50 of the extract was 13.51 µg/mL. The β-carotene bleaching test showed that the addition of the A. chica extract in different concentrations (200 and 500 µg/mL) prevented the bleaching of β-carotene at different degrees (51.2% ±3.38% and 94% ±4.61%, respectively). The TRAP test showed dose-dependent correlation between the increasing concentrations of A. chica extract (0.1, 0.5, and 1.0 µg/mL) and the TRAP values obtained by trolox (hydro-soluble vitamin E) 0.4738±0.0466, 1.981±0.1603, and 6.877±1.445 µM, respectively. The 2 main flavonoids, scutellarein and apigenin, were separated, and their antioxidant activity was found to be the same as that of the plant extract. These 2 flavonoids were quantified in the plant extract by using a validated HPLC-UV method. The results of these tests showed that the extract of A. chica had a significant antioxidant activity, which could be attributed to the presence of the mixture of flavonoids in the plant extract, with the main contribution of scutellarein and apigenin. |
| doi_str_mv | 10.1371/journal.pone.0072733 |
| format | Article |
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A. chica is known to be a good producer of phenolics. Therefore, in the present study, we investigated its antioxidant activity. The phenolic composition of A. chica leaves was studied by liquid chromatography coupled to diode array detection (LC-DAD) and liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), and isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein, luteolin, and apigenin were identified. The extract from leaves of A. chica was tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, β-carotene bleaching test, and total reactive antioxidant potential (TRAP) method. The crude extract quenched DPPH free radicals in a dose-dependent manner, and the IC50 of the extract was 13.51 µg/mL. The β-carotene bleaching test showed that the addition of the A. chica extract in different concentrations (200 and 500 µg/mL) prevented the bleaching of β-carotene at different degrees (51.2% ±3.38% and 94% ±4.61%, respectively). The TRAP test showed dose-dependent correlation between the increasing concentrations of A. chica extract (0.1, 0.5, and 1.0 µg/mL) and the TRAP values obtained by trolox (hydro-soluble vitamin E) 0.4738±0.0466, 1.981±0.1603, and 6.877±1.445 µM, respectively. The 2 main flavonoids, scutellarein and apigenin, were separated, and their antioxidant activity was found to be the same as that of the plant extract. These 2 flavonoids were quantified in the plant extract by using a validated HPLC-UV method. The results of these tests showed that the extract of A. chica had a significant antioxidant activity, which could be attributed to the presence of the mixture of flavonoids in the plant extract, with the main contribution of scutellarein and apigenin.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0072733</identifier><identifier>PMID: 24009700</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anemia ; Antioxidants ; Antioxidants - analysis ; Antioxidants - chemistry ; Antioxidants - pharmacology ; Arrabidaea chica ; Biology ; Bleaching ; Brazil ; Chemistry ; Chromatography ; Chromatography, High Pressure Liquid ; Colic ; Diarrhea ; Ethanol ; Flavonoids ; Food ; Free radicals ; Herbal medicine ; High performance liquid chromatography ; Inflammation ; Intestine ; Ionization ; Leaves ; Leukemia ; Liquid chromatography ; Mass Spectrometry ; Mass spectroscopy ; Metabolites ; Methods ; NMR ; Nuclear magnetic resonance ; Nuclear Magnetic Resonance, Biomolecular ; Pharmaceutical sciences ; Phenolic compounds ; Phenols ; Plant extracts ; Plant Extracts - analysis ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Quenching ; Reproducibility of Results ; Sensitivity and Specificity ; Studies ; Test procedures ; Tocopherol ; Tracheophyta - chemistry ; Vitamin E ; β-Carotene</subject><ispartof>PloS one, 2013-08, Vol.8 (8), p.e72733-e72733</ispartof><rights>2013 Siraichi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Siraichi et al 2013 Siraichi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-4b7e218f601dc349442e3224e179c335d60c58385f3216176e123468252c28223</citedby><cites>FETCH-LOGICAL-c526t-4b7e218f601dc349442e3224e179c335d60c58385f3216176e123468252c28223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757019/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757019/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24009700$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Harder, Tilmann</contributor><creatorcontrib>Siraichi, Jackeline Tiemy Guinoza</creatorcontrib><creatorcontrib>Felipe, Daniele Fernanda</creatorcontrib><creatorcontrib>Brambilla, Lara Zampar Serra</creatorcontrib><creatorcontrib>Gatto, Melissa Junqueira</creatorcontrib><creatorcontrib>Terra, Vânia Aparecida</creatorcontrib><creatorcontrib>Cecchini, Alessandra Lourenco</creatorcontrib><creatorcontrib>Cortez, Lucia Elaine Ranieri</creatorcontrib><creatorcontrib>Rodrigues-Filho, Edson</creatorcontrib><creatorcontrib>Cortez, Diógenes Aparício Garcia</creatorcontrib><title>Antioxidant capacity of the leaf extract obtained from Arrabidaea chica cultivated in Southern Brazil</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Arrabidaea chica leaf extract has been used by people as an anti-inflammatory and astringent agent as well as a remedy for intestinal colic, diarrhea, leucorrhea, anemia, and leukemia. A. chica is known to be a good producer of phenolics. Therefore, in the present study, we investigated its antioxidant activity. The phenolic composition of A. chica leaves was studied by liquid chromatography coupled to diode array detection (LC-DAD) and liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), and isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein, luteolin, and apigenin were identified. The extract from leaves of A. chica was tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, β-carotene bleaching test, and total reactive antioxidant potential (TRAP) method. The crude extract quenched DPPH free radicals in a dose-dependent manner, and the IC50 of the extract was 13.51 µg/mL. The β-carotene bleaching test showed that the addition of the A. chica extract in different concentrations (200 and 500 µg/mL) prevented the bleaching of β-carotene at different degrees (51.2% ±3.38% and 94% ±4.61%, respectively). The TRAP test showed dose-dependent correlation between the increasing concentrations of A. chica extract (0.1, 0.5, and 1.0 µg/mL) and the TRAP values obtained by trolox (hydro-soluble vitamin E) 0.4738±0.0466, 1.981±0.1603, and 6.877±1.445 µM, respectively. The 2 main flavonoids, scutellarein and apigenin, were separated, and their antioxidant activity was found to be the same as that of the plant extract. These 2 flavonoids were quantified in the plant extract by using a validated HPLC-UV method. The results of these tests showed that the extract of A. chica had a significant antioxidant activity, which could be attributed to the presence of the mixture of flavonoids in the plant extract, with the main contribution of scutellarein and apigenin.</description><subject>Anemia</subject><subject>Antioxidants</subject><subject>Antioxidants - analysis</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>Arrabidaea chica</subject><subject>Biology</subject><subject>Bleaching</subject><subject>Brazil</subject><subject>Chemistry</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Colic</subject><subject>Diarrhea</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Food</subject><subject>Free radicals</subject><subject>Herbal medicine</subject><subject>High performance liquid chromatography</subject><subject>Inflammation</subject><subject>Intestine</subject><subject>Ionization</subject><subject>Leaves</subject><subject>Leukemia</subject><subject>Liquid chromatography</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Methods</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>Pharmaceutical sciences</subject><subject>Phenolic compounds</subject><subject>Phenols</subject><subject>Plant extracts</subject><subject>Plant Extracts - analysis</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Quenching</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Studies</subject><subject>Test procedures</subject><subject>Tocopherol</subject><subject>Tracheophyta - chemistry</subject><subject>Vitamin E</subject><subject>β-Carotene</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptkl1vFCEUhonR2Lr6D4ySeOPNrnzNADcm28aPJk28UK8Jw5zpsmFhZZim9dfLutOmNd4Aged9OQdehF5TsqJc0g_bNOVow2qfIqwIkUxy_gSdUs3ZsmWEP32wPkEvxnFLSMNV2z5HJ0wQoiUhpwjWsfh043sbC3Z2b50vtzgNuGwAB7ADhpuSrSs4dcX6CD0ectrhdc62qyqw2G28q-MUir-2pQI-4u9pqgY54rNsf_vwEj0bbBjh1Twv0M_Pn36cf11efvtycb6-XLqGtWUpOgmMqqEltHdcaCEYcMYEUKkd503fEtcorpqBM9pS2QJlXLSKNcwxxRhfoLdH331Io5lfaDRUMKWIrHAlLo5En-zW7LPf2XxrkvXm70bKV8bm4l0AQ-SgG3BUVKlgQugOQOqeC6jVam2r18f5tqnbQe8g1pcKj0wfn0S_MVfp2nDZSFI_Z4HezwY5_ZpgLGbnRwch2AhpOtTNiWoqfOjs3T_o_7sTR8rlNI4ZhvtiKDGH1NypzCE1Zk5Nlb152Mi96C4m_A_UO76t</recordid><startdate>20130829</startdate><enddate>20130829</enddate><creator>Siraichi, Jackeline Tiemy Guinoza</creator><creator>Felipe, Daniele Fernanda</creator><creator>Brambilla, Lara Zampar Serra</creator><creator>Gatto, Melissa Junqueira</creator><creator>Terra, Vânia Aparecida</creator><creator>Cecchini, Alessandra Lourenco</creator><creator>Cortez, Lucia Elaine Ranieri</creator><creator>Rodrigues-Filho, Edson</creator><creator>Cortez, Diógenes Aparício Garcia</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130829</creationdate><title>Antioxidant capacity of the leaf extract obtained from Arrabidaea chica cultivated in Southern Brazil</title><author>Siraichi, Jackeline Tiemy Guinoza ; Felipe, Daniele Fernanda ; Brambilla, Lara Zampar Serra ; Gatto, Melissa Junqueira ; Terra, Vânia Aparecida ; Cecchini, Alessandra Lourenco ; Cortez, Lucia Elaine Ranieri ; Rodrigues-Filho, Edson ; Cortez, Diógenes Aparício Garcia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-4b7e218f601dc349442e3224e179c335d60c58385f3216176e123468252c28223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anemia</topic><topic>Antioxidants</topic><topic>Antioxidants - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siraichi, Jackeline Tiemy Guinoza</au><au>Felipe, Daniele Fernanda</au><au>Brambilla, Lara Zampar Serra</au><au>Gatto, Melissa Junqueira</au><au>Terra, Vânia Aparecida</au><au>Cecchini, Alessandra Lourenco</au><au>Cortez, Lucia Elaine Ranieri</au><au>Rodrigues-Filho, Edson</au><au>Cortez, Diógenes Aparício Garcia</au><au>Harder, Tilmann</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant capacity of the leaf extract obtained from Arrabidaea chica cultivated in Southern Brazil</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-08-29</date><risdate>2013</risdate><volume>8</volume><issue>8</issue><spage>e72733</spage><epage>e72733</epage><pages>e72733-e72733</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Arrabidaea chica leaf extract has been used by people as an anti-inflammatory and astringent agent as well as a remedy for intestinal colic, diarrhea, leucorrhea, anemia, and leukemia. A. chica is known to be a good producer of phenolics. Therefore, in the present study, we investigated its antioxidant activity. The phenolic composition of A. chica leaves was studied by liquid chromatography coupled to diode array detection (LC-DAD) and liquid chromatography coupled to electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), and isoscutellarein, 6-hydroxyluteolin, hispidulin, scutellarein, luteolin, and apigenin were identified. The extract from leaves of A. chica was tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, β-carotene bleaching test, and total reactive antioxidant potential (TRAP) method. The crude extract quenched DPPH free radicals in a dose-dependent manner, and the IC50 of the extract was 13.51 µg/mL. The β-carotene bleaching test showed that the addition of the A. chica extract in different concentrations (200 and 500 µg/mL) prevented the bleaching of β-carotene at different degrees (51.2% ±3.38% and 94% ±4.61%, respectively). The TRAP test showed dose-dependent correlation between the increasing concentrations of A. chica extract (0.1, 0.5, and 1.0 µg/mL) and the TRAP values obtained by trolox (hydro-soluble vitamin E) 0.4738±0.0466, 1.981±0.1603, and 6.877±1.445 µM, respectively. The 2 main flavonoids, scutellarein and apigenin, were separated, and their antioxidant activity was found to be the same as that of the plant extract. These 2 flavonoids were quantified in the plant extract by using a validated HPLC-UV method. The results of these tests showed that the extract of A. chica had a significant antioxidant activity, which could be attributed to the presence of the mixture of flavonoids in the plant extract, with the main contribution of scutellarein and apigenin.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24009700</pmid><doi>10.1371/journal.pone.0072733</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-08, Vol.8 (8), p.e72733-e72733 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1428807123 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Anemia Antioxidants Antioxidants - analysis Antioxidants - chemistry Antioxidants - pharmacology Arrabidaea chica Biology Bleaching Brazil Chemistry Chromatography Chromatography, High Pressure Liquid Colic Diarrhea Ethanol Flavonoids Food Free radicals Herbal medicine High performance liquid chromatography Inflammation Intestine Ionization Leaves Leukemia Liquid chromatography Mass Spectrometry Mass spectroscopy Metabolites Methods NMR Nuclear magnetic resonance Nuclear Magnetic Resonance, Biomolecular Pharmaceutical sciences Phenolic compounds Phenols Plant extracts Plant Extracts - analysis Plant Extracts - chemistry Plant Extracts - pharmacology Plant Leaves - chemistry Quenching Reproducibility of Results Sensitivity and Specificity Studies Test procedures Tocopherol Tracheophyta - chemistry Vitamin E β-Carotene |
| title | Antioxidant capacity of the leaf extract obtained from Arrabidaea chica cultivated in Southern Brazil |
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