Newcastle disease virus fusion protein is the major contributor to protective immunity of genotype-matched vaccine
Virulent strains of Newcastle disease virus (NDV) can cause devastating disease in chickens worldwide. Although the current vaccines are substantially effective, they do not completely prevent infection, virus shedding and disease. To produce genotype-matched vaccines, a full-genome reverse genetics...
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| creator | Kim, Shin-Hee Wanasen, Nanchaya Paldurai, Anandan Xiao, Sa Collins, Peter L Samal, Siba K |
| description | Virulent strains of Newcastle disease virus (NDV) can cause devastating disease in chickens worldwide. Although the current vaccines are substantially effective, they do not completely prevent infection, virus shedding and disease. To produce genotype-matched vaccines, a full-genome reverse genetics system has been used to generate a recombinant virus in which the F protein cleavage site has been changed to that of avirulent vaccine virus. In the other strategy, the vaccines have been generated by replacing the F and HN genes of a commercial vaccine strain with those from a genotype-matched virus. However, the protective efficacy of a chimeric virus vaccine has not been directly compared with that of a full-genome virus vaccine developed by reverse genetics. Therefore, in this study, we evaluated the protective efficacy of genotype VII matched chimeric vaccines by generating three recombinant viruses based on avirulent LaSota (genotype II) strain in which the open reading frames (ORFs) encoding the F and HN proteins were replaced, individually or together, with those of the circulating and highly virulent Indonesian NDV strain Ban/010. The cleavage site of the Ban/010 F protein was mutated to the avirulent motif found in strain LaSota. In vitro growth characteristics and a pathogenicity test indicated that all three chimeric viruses retained the highly attenuated phenotype of the parental viruses. Immunization of chickens with chimeric and full-length genome VII vaccines followed by challenge with virulent Ban/010 or Texas GB (genotype II) virus demonstrated protection against clinical disease and death. However, only those chickens immunized with chimeric rLaSota expressing the F or F plus HN proteins of the Indonesian strain were efficiently protected against shedding of Ban/010 virus. Our findings showed that genotype-matched vaccines can provide protection to chickens by efficiently preventing spread of virus, primarily due to the F protein. |
| doi_str_mv | 10.1371/journal.pone.0074022 |
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Although the current vaccines are substantially effective, they do not completely prevent infection, virus shedding and disease. To produce genotype-matched vaccines, a full-genome reverse genetics system has been used to generate a recombinant virus in which the F protein cleavage site has been changed to that of avirulent vaccine virus. In the other strategy, the vaccines have been generated by replacing the F and HN genes of a commercial vaccine strain with those from a genotype-matched virus. However, the protective efficacy of a chimeric virus vaccine has not been directly compared with that of a full-genome virus vaccine developed by reverse genetics. Therefore, in this study, we evaluated the protective efficacy of genotype VII matched chimeric vaccines by generating three recombinant viruses based on avirulent LaSota (genotype II) strain in which the open reading frames (ORFs) encoding the F and HN proteins were replaced, individually or together, with those of the circulating and highly virulent Indonesian NDV strain Ban/010. The cleavage site of the Ban/010 F protein was mutated to the avirulent motif found in strain LaSota. In vitro growth characteristics and a pathogenicity test indicated that all three chimeric viruses retained the highly attenuated phenotype of the parental viruses. Immunization of chickens with chimeric and full-length genome VII vaccines followed by challenge with virulent Ban/010 or Texas GB (genotype II) virus demonstrated protection against clinical disease and death. However, only those chickens immunized with chimeric rLaSota expressing the F or F plus HN proteins of the Indonesian strain were efficiently protected against shedding of Ban/010 virus. Our findings showed that genotype-matched vaccines can provide protection to chickens by efficiently preventing spread of virus, primarily due to the F protein.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0074022</identifier><identifier>PMID: 24015313</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal vaccines ; Animals ; Cell Line, Tumor ; Chick Embryo ; Chickens ; Cleavage ; F protein ; Fusion protein ; Genetics ; Genomes ; Humans ; Immunity ; Immunity (Disease) ; Immunization ; Mutation ; Newcastle disease ; Newcastle Disease - genetics ; Newcastle Disease - immunology ; Newcastle Disease - prevention & control ; Newcastle Disease - transmission ; Newcastle disease virus - genetics ; Newcastle disease virus - immunology ; Open reading frames ; Pathogenicity ; Pathogens ; Poultry ; Proteins ; Shedding ; Vaccines ; Veterinary colleges ; Viral Fusion Proteins - genetics ; Viral Fusion Proteins - immunology ; Viral Vaccines - genetics ; Viral Vaccines - immunology ; Viral Vaccines - pharmacology ; Viruses</subject><ispartof>PloS one, 2013-08, Vol.8 (8), p.e74022-e74022</ispartof><rights>2013. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-aeac38e494b16f93d52dd8a8e25a65659f4c8d77fac456dd47ecab28f74fcd8a3</citedby><cites>FETCH-LOGICAL-c526t-aeac38e494b16f93d52dd8a8e25a65659f4c8d77fac456dd47ecab28f74fcd8a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3755997/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3755997/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24015313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Shin-Hee</creatorcontrib><creatorcontrib>Wanasen, Nanchaya</creatorcontrib><creatorcontrib>Paldurai, Anandan</creatorcontrib><creatorcontrib>Xiao, Sa</creatorcontrib><creatorcontrib>Collins, Peter L</creatorcontrib><creatorcontrib>Samal, Siba K</creatorcontrib><title>Newcastle disease virus fusion protein is the major contributor to protective immunity of genotype-matched vaccine</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Virulent strains of Newcastle disease virus (NDV) can cause devastating disease in chickens worldwide. Although the current vaccines are substantially effective, they do not completely prevent infection, virus shedding and disease. To produce genotype-matched vaccines, a full-genome reverse genetics system has been used to generate a recombinant virus in which the F protein cleavage site has been changed to that of avirulent vaccine virus. In the other strategy, the vaccines have been generated by replacing the F and HN genes of a commercial vaccine strain with those from a genotype-matched virus. However, the protective efficacy of a chimeric virus vaccine has not been directly compared with that of a full-genome virus vaccine developed by reverse genetics. Therefore, in this study, we evaluated the protective efficacy of genotype VII matched chimeric vaccines by generating three recombinant viruses based on avirulent LaSota (genotype II) strain in which the open reading frames (ORFs) encoding the F and HN proteins were replaced, individually or together, with those of the circulating and highly virulent Indonesian NDV strain Ban/010. The cleavage site of the Ban/010 F protein was mutated to the avirulent motif found in strain LaSota. In vitro growth characteristics and a pathogenicity test indicated that all three chimeric viruses retained the highly attenuated phenotype of the parental viruses. Immunization of chickens with chimeric and full-length genome VII vaccines followed by challenge with virulent Ban/010 or Texas GB (genotype II) virus demonstrated protection against clinical disease and death. However, only those chickens immunized with chimeric rLaSota expressing the F or F plus HN proteins of the Indonesian strain were efficiently protected against shedding of Ban/010 virus. Our findings showed that genotype-matched vaccines can provide protection to chickens by efficiently preventing spread of virus, primarily due to the F protein.</description><subject>Animal vaccines</subject><subject>Animals</subject><subject>Cell Line, Tumor</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Cleavage</subject><subject>F protein</subject><subject>Fusion protein</subject><subject>Genetics</subject><subject>Genomes</subject><subject>Humans</subject><subject>Immunity</subject><subject>Immunity (Disease)</subject><subject>Immunization</subject><subject>Mutation</subject><subject>Newcastle disease</subject><subject>Newcastle Disease - genetics</subject><subject>Newcastle Disease - immunology</subject><subject>Newcastle Disease - prevention & control</subject><subject>Newcastle Disease - transmission</subject><subject>Newcastle disease virus - genetics</subject><subject>Newcastle disease virus - immunology</subject><subject>Open reading frames</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Poultry</subject><subject>Proteins</subject><subject>Shedding</subject><subject>Vaccines</subject><subject>Veterinary colleges</subject><subject>Viral Fusion Proteins - 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genetics</topic><topic>Newcastle Disease - immunology</topic><topic>Newcastle Disease - prevention & control</topic><topic>Newcastle Disease - transmission</topic><topic>Newcastle disease virus - genetics</topic><topic>Newcastle disease virus - immunology</topic><topic>Open reading frames</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Poultry</topic><topic>Proteins</topic><topic>Shedding</topic><topic>Vaccines</topic><topic>Veterinary colleges</topic><topic>Viral Fusion Proteins - genetics</topic><topic>Viral Fusion Proteins - immunology</topic><topic>Viral Vaccines - genetics</topic><topic>Viral Vaccines - immunology</topic><topic>Viral Vaccines - pharmacology</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Shin-Hee</creatorcontrib><creatorcontrib>Wanasen, Nanchaya</creatorcontrib><creatorcontrib>Paldurai, Anandan</creatorcontrib><creatorcontrib>Xiao, Sa</creatorcontrib><creatorcontrib>Collins, Peter L</creatorcontrib><creatorcontrib>Samal, Siba K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Shin-Hee</au><au>Wanasen, Nanchaya</au><au>Paldurai, Anandan</au><au>Xiao, Sa</au><au>Collins, Peter L</au><au>Samal, Siba K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Newcastle disease virus fusion protein is the major contributor to protective immunity of genotype-matched vaccine</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-08-28</date><risdate>2013</risdate><volume>8</volume><issue>8</issue><spage>e74022</spage><epage>e74022</epage><pages>e74022-e74022</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Virulent strains of Newcastle disease virus (NDV) can cause devastating disease in chickens worldwide. Although the current vaccines are substantially effective, they do not completely prevent infection, virus shedding and disease. To produce genotype-matched vaccines, a full-genome reverse genetics system has been used to generate a recombinant virus in which the F protein cleavage site has been changed to that of avirulent vaccine virus. In the other strategy, the vaccines have been generated by replacing the F and HN genes of a commercial vaccine strain with those from a genotype-matched virus. However, the protective efficacy of a chimeric virus vaccine has not been directly compared with that of a full-genome virus vaccine developed by reverse genetics. Therefore, in this study, we evaluated the protective efficacy of genotype VII matched chimeric vaccines by generating three recombinant viruses based on avirulent LaSota (genotype II) strain in which the open reading frames (ORFs) encoding the F and HN proteins were replaced, individually or together, with those of the circulating and highly virulent Indonesian NDV strain Ban/010. The cleavage site of the Ban/010 F protein was mutated to the avirulent motif found in strain LaSota. In vitro growth characteristics and a pathogenicity test indicated that all three chimeric viruses retained the highly attenuated phenotype of the parental viruses. Immunization of chickens with chimeric and full-length genome VII vaccines followed by challenge with virulent Ban/010 or Texas GB (genotype II) virus demonstrated protection against clinical disease and death. However, only those chickens immunized with chimeric rLaSota expressing the F or F plus HN proteins of the Indonesian strain were efficiently protected against shedding of Ban/010 virus. Our findings showed that genotype-matched vaccines can provide protection to chickens by efficiently preventing spread of virus, primarily due to the F protein.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24015313</pmid><doi>10.1371/journal.pone.0074022</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animal vaccines Animals Cell Line, Tumor Chick Embryo Chickens Cleavage F protein Fusion protein Genetics Genomes Humans Immunity Immunity (Disease) Immunization Mutation Newcastle disease Newcastle Disease - genetics Newcastle Disease - immunology Newcastle Disease - prevention & control Newcastle Disease - transmission Newcastle disease virus - genetics Newcastle disease virus - immunology Open reading frames Pathogenicity Pathogens Poultry Proteins Shedding Vaccines Veterinary colleges Viral Fusion Proteins - genetics Viral Fusion Proteins - immunology Viral Vaccines - genetics Viral Vaccines - immunology Viral Vaccines - pharmacology Viruses |
| title | Newcastle disease virus fusion protein is the major contributor to protective immunity of genotype-matched vaccine |
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