Track-a-worm, an open-source system for quantitative assessment of C. elegans locomotory and bending behavior
A major challenge of neuroscience is to understand the circuit and gene bases of behavior. C. elegans is commonly used as a model system to investigate how various gene products function at specific tissue, cellular, and synaptic foci to produce complicated locomotory and bending behavior. The inves...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-07, Vol.8 (7), p.e69653-e69653 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e69653 |
|---|---|
| container_issue | 7 |
| container_start_page | e69653 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Wang, Sijie Jason Wang, Zhao-Wen |
| description | A major challenge of neuroscience is to understand the circuit and gene bases of behavior. C. elegans is commonly used as a model system to investigate how various gene products function at specific tissue, cellular, and synaptic foci to produce complicated locomotory and bending behavior. The investigation generally requires quantitative behavioral analyses using an automated single-worm tracker, which constantly records and analyzes the position and body shape of a freely moving worm at a high magnification. Many single-worm trackers have been developed to meet lab-specific needs, but none has been widely implemented for various reasons, such as hardware difficult to assemble, and software lacking sufficient functionality, having closed source code, or using a programming language that is not broadly accessible. The lack of a versatile system convenient for wide implementation makes data comparisons difficult and compels other labs to develop new worm trackers. Here we describe Track-A-Worm, a system rich in functionality, open in source code, and easy to use. The system includes plug-and-play hardware (a stereomicroscope, a digital camera and a motorized stage), custom software written to run with Matlab in Windows 7, and a detailed user manual. Grayscale images are automatically converted to binary images followed by head identification and placement of 13 markers along a deduced spline. The software can extract and quantify a variety of parameters, including distance traveled, average speed, distance/time/speed of forward and backward locomotion, frequency and amplitude of dominant bends, overall bending activities measured as root mean square, and sum of all bends. It also plots worm travel path, bend trace, and bend frequency spectrum. All functionality is performed through graphical user interfaces and data is exported to clearly-annotated and documented Excel files. These features make Track-A-Worm a good candidate for implementation in other labs. |
| doi_str_mv | 10.1371/journal.pone.0069653 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1428143606</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_164fc5841b1446e58c52ca35167842f2</doaj_id><sourcerecordid>3054536671</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-90df4e831630844dfbd9a7588e97ce66df0b307b981109491d738e0533962ff13</originalsourceid><addsrcrecordid>eNptkktv1DAUhSMEoqXwDxBYYsOCDH7FsTdIaMSjUiU2ZW05zvU0g2NP7WTQ_Hs8TFq1iNW17HM_n3t1quo1wSvCWvJxG-cUjF_tYoAVxkKJhj2pzolitBYUs6cPzmfVi5y3GDdMCvG8OqNMUdoKdV6N18nYX7Wpf8c0fkAmoLiDUOcCt4DyIU8wIhcTup1NmIbJTMMekMkZch4hTCg6tF4h8LAxISMfbRzjFNOhoHrUQeiHsCn1xuyHmF5Wz5zxGV4t9aL6-fXL9fp7ffXj2-X681VtGyqmWuHecZCMCIYl573remXaRkpQrQUheoc7httOSUKw4or0LZNQpmNKUOcIu6jenrg7H7NeNpU14VQSzgQWRXF5UvTRbPUuDaNJBx3NoP9exLTRJk2D9aCJ4M42kpOOcC6gkcWkNawhopWcOlpYn5bf5m6E3pa1JOMfQR-_hOFGb-Jes5ZyxVgBvF8AKd7OkCc9DtmC9yZAnI--iRRcKN4U6bt_pP-fjp9UNsWcE7h7MwTrY3ruuvQxPXpJT2l783CQ-6a7uLA_ZHXCsw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1428143606</pqid></control><display><type>article</type><title>Track-a-worm, an open-source system for quantitative assessment of C. elegans locomotory and bending behavior</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Wang, Sijie Jason ; Wang, Zhao-Wen</creator><contributor>Gilestro, Giorgio F.</contributor><creatorcontrib>Wang, Sijie Jason ; Wang, Zhao-Wen ; Gilestro, Giorgio F.</creatorcontrib><description>A major challenge of neuroscience is to understand the circuit and gene bases of behavior. C. elegans is commonly used as a model system to investigate how various gene products function at specific tissue, cellular, and synaptic foci to produce complicated locomotory and bending behavior. The investigation generally requires quantitative behavioral analyses using an automated single-worm tracker, which constantly records and analyzes the position and body shape of a freely moving worm at a high magnification. Many single-worm trackers have been developed to meet lab-specific needs, but none has been widely implemented for various reasons, such as hardware difficult to assemble, and software lacking sufficient functionality, having closed source code, or using a programming language that is not broadly accessible. The lack of a versatile system convenient for wide implementation makes data comparisons difficult and compels other labs to develop new worm trackers. Here we describe Track-A-Worm, a system rich in functionality, open in source code, and easy to use. The system includes plug-and-play hardware (a stereomicroscope, a digital camera and a motorized stage), custom software written to run with Matlab in Windows 7, and a detailed user manual. Grayscale images are automatically converted to binary images followed by head identification and placement of 13 markers along a deduced spline. The software can extract and quantify a variety of parameters, including distance traveled, average speed, distance/time/speed of forward and backward locomotion, frequency and amplitude of dominant bends, overall bending activities measured as root mean square, and sum of all bends. It also plots worm travel path, bend trace, and bend frequency spectrum. All functionality is performed through graphical user interfaces and data is exported to clearly-annotated and documented Excel files. These features make Track-A-Worm a good candidate for implementation in other labs.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0069653</identifier><identifier>PMID: 23922769</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Automation ; Behavior, Animal - physiology ; Bends ; Biology ; Caenorhabditis elegans ; Caenorhabditis elegans - physiology ; Calibration ; Computer programs ; Computer Science ; Digital cameras ; Engineering ; Frequency spectrum ; Graphical user interface ; Hardware ; Imaging, Three-Dimensional ; Interfaces ; Localization ; Locomotion ; Locomotion - physiology ; Nematodes ; Nervous system ; Neurons ; Operating systems ; Programming languages ; Software ; Source code ; User interfaces ; Worms</subject><ispartof>PloS one, 2013-07, Vol.8 (7), p.e69653-e69653</ispartof><rights>2013 Wang, Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Wang, Wang 2013 Wang, Wang</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-90df4e831630844dfbd9a7588e97ce66df0b307b981109491d738e0533962ff13</citedby><cites>FETCH-LOGICAL-c526t-90df4e831630844dfbd9a7588e97ce66df0b307b981109491d738e0533962ff13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724933/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724933/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23849,27907,27908,53774,53776,79351,79352</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23922769$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gilestro, Giorgio F.</contributor><creatorcontrib>Wang, Sijie Jason</creatorcontrib><creatorcontrib>Wang, Zhao-Wen</creatorcontrib><title>Track-a-worm, an open-source system for quantitative assessment of C. elegans locomotory and bending behavior</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A major challenge of neuroscience is to understand the circuit and gene bases of behavior. C. elegans is commonly used as a model system to investigate how various gene products function at specific tissue, cellular, and synaptic foci to produce complicated locomotory and bending behavior. The investigation generally requires quantitative behavioral analyses using an automated single-worm tracker, which constantly records and analyzes the position and body shape of a freely moving worm at a high magnification. Many single-worm trackers have been developed to meet lab-specific needs, but none has been widely implemented for various reasons, such as hardware difficult to assemble, and software lacking sufficient functionality, having closed source code, or using a programming language that is not broadly accessible. The lack of a versatile system convenient for wide implementation makes data comparisons difficult and compels other labs to develop new worm trackers. Here we describe Track-A-Worm, a system rich in functionality, open in source code, and easy to use. The system includes plug-and-play hardware (a stereomicroscope, a digital camera and a motorized stage), custom software written to run with Matlab in Windows 7, and a detailed user manual. Grayscale images are automatically converted to binary images followed by head identification and placement of 13 markers along a deduced spline. The software can extract and quantify a variety of parameters, including distance traveled, average speed, distance/time/speed of forward and backward locomotion, frequency and amplitude of dominant bends, overall bending activities measured as root mean square, and sum of all bends. It also plots worm travel path, bend trace, and bend frequency spectrum. All functionality is performed through graphical user interfaces and data is exported to clearly-annotated and documented Excel files. These features make Track-A-Worm a good candidate for implementation in other labs.</description><subject>Animals</subject><subject>Automation</subject><subject>Behavior, Animal - physiology</subject><subject>Bends</subject><subject>Biology</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - physiology</subject><subject>Calibration</subject><subject>Computer programs</subject><subject>Computer Science</subject><subject>Digital cameras</subject><subject>Engineering</subject><subject>Frequency spectrum</subject><subject>Graphical user interface</subject><subject>Hardware</subject><subject>Imaging, Three-Dimensional</subject><subject>Interfaces</subject><subject>Localization</subject><subject>Locomotion</subject><subject>Locomotion - physiology</subject><subject>Nematodes</subject><subject>Nervous system</subject><subject>Neurons</subject><subject>Operating systems</subject><subject>Programming languages</subject><subject>Software</subject><subject>Source code</subject><subject>User interfaces</subject><subject>Worms</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptkktv1DAUhSMEoqXwDxBYYsOCDH7FsTdIaMSjUiU2ZW05zvU0g2NP7WTQ_Hs8TFq1iNW17HM_n3t1quo1wSvCWvJxG-cUjF_tYoAVxkKJhj2pzolitBYUs6cPzmfVi5y3GDdMCvG8OqNMUdoKdV6N18nYX7Wpf8c0fkAmoLiDUOcCt4DyIU8wIhcTup1NmIbJTMMekMkZch4hTCg6tF4h8LAxISMfbRzjFNOhoHrUQeiHsCn1xuyHmF5Wz5zxGV4t9aL6-fXL9fp7ffXj2-X681VtGyqmWuHecZCMCIYl573remXaRkpQrQUheoc7httOSUKw4or0LZNQpmNKUOcIu6jenrg7H7NeNpU14VQSzgQWRXF5UvTRbPUuDaNJBx3NoP9exLTRJk2D9aCJ4M42kpOOcC6gkcWkNawhopWcOlpYn5bf5m6E3pa1JOMfQR-_hOFGb-Jes5ZyxVgBvF8AKd7OkCc9DtmC9yZAnI--iRRcKN4U6bt_pP-fjp9UNsWcE7h7MwTrY3ruuvQxPXpJT2l783CQ-6a7uLA_ZHXCsw</recordid><startdate>20130726</startdate><enddate>20130726</enddate><creator>Wang, Sijie Jason</creator><creator>Wang, Zhao-Wen</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130726</creationdate><title>Track-a-worm, an open-source system for quantitative assessment of C. elegans locomotory and bending behavior</title><author>Wang, Sijie Jason ; Wang, Zhao-Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-90df4e831630844dfbd9a7588e97ce66df0b307b981109491d738e0533962ff13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Automation</topic><topic>Behavior, Animal - physiology</topic><topic>Bends</topic><topic>Biology</topic><topic>Caenorhabditis elegans</topic><topic>Caenorhabditis elegans - physiology</topic><topic>Calibration</topic><topic>Computer programs</topic><topic>Computer Science</topic><topic>Digital cameras</topic><topic>Engineering</topic><topic>Frequency spectrum</topic><topic>Graphical user interface</topic><topic>Hardware</topic><topic>Imaging, Three-Dimensional</topic><topic>Interfaces</topic><topic>Localization</topic><topic>Locomotion</topic><topic>Locomotion - physiology</topic><topic>Nematodes</topic><topic>Nervous system</topic><topic>Neurons</topic><topic>Operating systems</topic><topic>Programming languages</topic><topic>Software</topic><topic>Source code</topic><topic>User interfaces</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Sijie Jason</creatorcontrib><creatorcontrib>Wang, Zhao-Wen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Sijie Jason</au><au>Wang, Zhao-Wen</au><au>Gilestro, Giorgio F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Track-a-worm, an open-source system for quantitative assessment of C. elegans locomotory and bending behavior</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-07-26</date><risdate>2013</risdate><volume>8</volume><issue>7</issue><spage>e69653</spage><epage>e69653</epage><pages>e69653-e69653</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>A major challenge of neuroscience is to understand the circuit and gene bases of behavior. C. elegans is commonly used as a model system to investigate how various gene products function at specific tissue, cellular, and synaptic foci to produce complicated locomotory and bending behavior. The investigation generally requires quantitative behavioral analyses using an automated single-worm tracker, which constantly records and analyzes the position and body shape of a freely moving worm at a high magnification. Many single-worm trackers have been developed to meet lab-specific needs, but none has been widely implemented for various reasons, such as hardware difficult to assemble, and software lacking sufficient functionality, having closed source code, or using a programming language that is not broadly accessible. The lack of a versatile system convenient for wide implementation makes data comparisons difficult and compels other labs to develop new worm trackers. Here we describe Track-A-Worm, a system rich in functionality, open in source code, and easy to use. The system includes plug-and-play hardware (a stereomicroscope, a digital camera and a motorized stage), custom software written to run with Matlab in Windows 7, and a detailed user manual. Grayscale images are automatically converted to binary images followed by head identification and placement of 13 markers along a deduced spline. The software can extract and quantify a variety of parameters, including distance traveled, average speed, distance/time/speed of forward and backward locomotion, frequency and amplitude of dominant bends, overall bending activities measured as root mean square, and sum of all bends. It also plots worm travel path, bend trace, and bend frequency spectrum. All functionality is performed through graphical user interfaces and data is exported to clearly-annotated and documented Excel files. These features make Track-A-Worm a good candidate for implementation in other labs.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23922769</pmid><doi>10.1371/journal.pone.0069653</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-07, Vol.8 (7), p.e69653-e69653 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1428143606 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Automation Behavior, Animal - physiology Bends Biology Caenorhabditis elegans Caenorhabditis elegans - physiology Calibration Computer programs Computer Science Digital cameras Engineering Frequency spectrum Graphical user interface Hardware Imaging, Three-Dimensional Interfaces Localization Locomotion Locomotion - physiology Nematodes Nervous system Neurons Operating systems Programming languages Software Source code User interfaces Worms |
| title | Track-a-worm, an open-source system for quantitative assessment of C. elegans locomotory and bending behavior |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T18%3A28%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Track-a-worm,%20an%20open-source%20system%20for%20quantitative%20assessment%20of%20C.%20elegans%20locomotory%20and%20bending%20behavior&rft.jtitle=PloS%20one&rft.au=Wang,%20Sijie%20Jason&rft.date=2013-07-26&rft.volume=8&rft.issue=7&rft.spage=e69653&rft.epage=e69653&rft.pages=e69653-e69653&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0069653&rft_dat=%3Cproquest_plos_%3E3054536671%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1428143606&rft_id=info:pmid/23922769&rft_doaj_id=oai_doaj_org_article_164fc5841b1446e58c52ca35167842f2&rfr_iscdi=true |