Probiotic properties of Lactobacillus strains isolated from Tibetan kefir grains
The objective of this study was to evaluate the functional properties of lactic acid bacteria (LAB) isolated from Tibetan kefir grains. Three Lactobacillus isolates identified as Lactobacillus acidophilus LA15, Lactobacillus plantarum B23 and Lactobacillus kefiri D17 that showed resistance to acid a...
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| Veröffentlicht in: | PloS one 2013-07, Vol.8 (7), p.e69868-e69868 |
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| creator | Zheng, Yongchen Lu, Yingli Wang, Jinfeng Yang, Longfei Pan, Chenyu Huang, Ying |
| description | The objective of this study was to evaluate the functional properties of lactic acid bacteria (LAB) isolated from Tibetan kefir grains. Three Lactobacillus isolates identified as Lactobacillus acidophilus LA15, Lactobacillus plantarum B23 and Lactobacillus kefiri D17 that showed resistance to acid and bile salts were selected for further evaluation of their probiotic properties. The 3 selected strains expressed high in vitro adherence to Caco-2 cells. They were sensitive to gentamicin, erythromycin and chloramphenicol and resistant to vancomycin with MIC values of 26 µg/ml. All 3 strains showed potential bile salt hydrolase (BSH) activity, cholesterol assimilation and cholesterol co-precipitation ability. Additionally, the potential effect of these strains on plasma cholesterol levels was evaluated in Sprague-Dawley (SD) rats. Rats in 4 treatment groups were fed the following experimental diets for 4 weeks: a high-cholesterol diet, a high-cholesterol diet plus LA15, a high-cholesterol diet plus B23 or a high-cholesterol diet plus D17. The total cholesterol, triglyceride and low-density lipoprotein cholesterol levels in the serum were significantly (P |
| doi_str_mv | 10.1371/journal.pone.0069868 |
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Three Lactobacillus isolates identified as Lactobacillus acidophilus LA15, Lactobacillus plantarum B23 and Lactobacillus kefiri D17 that showed resistance to acid and bile salts were selected for further evaluation of their probiotic properties. The 3 selected strains expressed high in vitro adherence to Caco-2 cells. They were sensitive to gentamicin, erythromycin and chloramphenicol and resistant to vancomycin with MIC values of 26 µg/ml. All 3 strains showed potential bile salt hydrolase (BSH) activity, cholesterol assimilation and cholesterol co-precipitation ability. Additionally, the potential effect of these strains on plasma cholesterol levels was evaluated in Sprague-Dawley (SD) rats. Rats in 4 treatment groups were fed the following experimental diets for 4 weeks: a high-cholesterol diet, a high-cholesterol diet plus LA15, a high-cholesterol diet plus B23 or a high-cholesterol diet plus D17. The total cholesterol, triglyceride and low-density lipoprotein cholesterol levels in the serum were significantly (P<0.05) decreased in the LAB-treated rats compared with rats fed a high-cholesterol diet without LAB supplementation. The high-density lipoprotein cholesterol levels in groups B23 and D17 were significantly (P<0.05) higher than those in the control and LA15 groups. Additionally, both fecal cholesterol and bile acid levels were significantly (P<0.05) increased after LAB administration. Fecal lactobacilli counts were significantly (P<0.05) higher in the LAB treatment groups than in the control groups. Furthermore, the 3 strains were detected in the rat small intestine, colon and feces during the feeding trial. The bacteria levels remained high even after the LAB administration had been stopped for 2 weeks. These results suggest that these strains may be used in the future as probiotic starter cultures for manufacturing novel fermented foods.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0069868</identifier><identifier>PMID: 23894554</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acid resistance ; Acids ; Analysis ; Animals ; Anti-Bacterial Agents - pharmacology ; Antibiotics ; Bacteria ; Bacterial Adhesion ; Bile ; Bile acids ; Bile Acids and Salts - metabolism ; Bile salts ; Biology ; Body Weight - drug effects ; Caco-2 Cells ; Chloramphenicol ; Cholesterol ; Cholesterol - blood ; Cholesterol - metabolism ; Colon ; Cultured Milk Products - microbiology ; Dietary supplements ; Drug resistance ; Drug Resistance, Bacterial ; Eating - drug effects ; Edible Grain - microbiology ; Erythromycin ; Feces - microbiology ; Fermented food ; Gentamicin ; Grains ; High cholesterol diet ; Humans ; Hydrogen-Ion Concentration ; Hydrolase ; Hypolipidemic Agents - metabolism ; Hypolipidemic Agents - pharmacology ; Intestines - microbiology ; Kefir ; Laboratories ; Lactic acid ; Lactic acid bacteria ; Lactobacilli ; Lactobacillus ; Lactobacillus - drug effects ; Lactobacillus - isolation & purification ; Lactobacillus - metabolism ; Lactobacillus - physiology ; Lactobacillus acidophilus ; Lactobacillus plantarum ; Liver - drug effects ; Liver - metabolism ; Low density lipoproteins ; Male ; Milk ; Minimum inhibitory concentration ; Precipitation (Meteorology) ; Probiotics ; Probiotics - metabolism ; Probiotics - pharmacology ; Rats ; Rats, Sprague-Dawley ; Ribosomal DNA ; Rodents ; Salt ; Salts ; Small intestine ; Species Specificity ; Starter cultures ; Strains (organisms) ; Tibet ; Vancomycin ; Yogurt</subject><ispartof>PloS one, 2013-07, Vol.8 (7), p.e69868-e69868</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Zheng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Zheng et al 2013 Zheng et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6738-d6d6c18774fd35647d98747dee905046a8551d7c3451cd8f92604791ad9f466a3</citedby><cites>FETCH-LOGICAL-c6738-d6d6c18774fd35647d98747dee905046a8551d7c3451cd8f92604791ad9f466a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718794/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718794/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23894554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lobaccaro, Jean-Marc A.</contributor><creatorcontrib>Zheng, Yongchen</creatorcontrib><creatorcontrib>Lu, Yingli</creatorcontrib><creatorcontrib>Wang, Jinfeng</creatorcontrib><creatorcontrib>Yang, Longfei</creatorcontrib><creatorcontrib>Pan, Chenyu</creatorcontrib><creatorcontrib>Huang, Ying</creatorcontrib><title>Probiotic properties of Lactobacillus strains isolated from Tibetan kefir grains</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The objective of this study was to evaluate the functional properties of lactic acid bacteria (LAB) isolated from Tibetan kefir grains. Three Lactobacillus isolates identified as Lactobacillus acidophilus LA15, Lactobacillus plantarum B23 and Lactobacillus kefiri D17 that showed resistance to acid and bile salts were selected for further evaluation of their probiotic properties. The 3 selected strains expressed high in vitro adherence to Caco-2 cells. They were sensitive to gentamicin, erythromycin and chloramphenicol and resistant to vancomycin with MIC values of 26 µg/ml. All 3 strains showed potential bile salt hydrolase (BSH) activity, cholesterol assimilation and cholesterol co-precipitation ability. Additionally, the potential effect of these strains on plasma cholesterol levels was evaluated in Sprague-Dawley (SD) rats. Rats in 4 treatment groups were fed the following experimental diets for 4 weeks: a high-cholesterol diet, a high-cholesterol diet plus LA15, a high-cholesterol diet plus B23 or a high-cholesterol diet plus D17. The total cholesterol, triglyceride and low-density lipoprotein cholesterol levels in the serum were significantly (P<0.05) decreased in the LAB-treated rats compared with rats fed a high-cholesterol diet without LAB supplementation. The high-density lipoprotein cholesterol levels in groups B23 and D17 were significantly (P<0.05) higher than those in the control and LA15 groups. Additionally, both fecal cholesterol and bile acid levels were significantly (P<0.05) increased after LAB administration. Fecal lactobacilli counts were significantly (P<0.05) higher in the LAB treatment groups than in the control groups. Furthermore, the 3 strains were detected in the rat small intestine, colon and feces during the feeding trial. The bacteria levels remained high even after the LAB administration had been stopped for 2 weeks. These results suggest that these strains may be used in the future as probiotic starter cultures for manufacturing novel fermented foods.</description><subject>Acid resistance</subject><subject>Acids</subject><subject>Analysis</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial Adhesion</subject><subject>Bile</subject><subject>Bile acids</subject><subject>Bile Acids and Salts - metabolism</subject><subject>Bile salts</subject><subject>Biology</subject><subject>Body Weight - drug effects</subject><subject>Caco-2 Cells</subject><subject>Chloramphenicol</subject><subject>Cholesterol</subject><subject>Cholesterol - blood</subject><subject>Cholesterol - metabolism</subject><subject>Colon</subject><subject>Cultured Milk Products - microbiology</subject><subject>Dietary supplements</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial</subject><subject>Eating - drug effects</subject><subject>Edible Grain - microbiology</subject><subject>Erythromycin</subject><subject>Feces - microbiology</subject><subject>Fermented food</subject><subject>Gentamicin</subject><subject>Grains</subject><subject>High cholesterol diet</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolase</subject><subject>Hypolipidemic Agents - metabolism</subject><subject>Hypolipidemic Agents - pharmacology</subject><subject>Intestines - microbiology</subject><subject>Kefir</subject><subject>Laboratories</subject><subject>Lactic acid</subject><subject>Lactic acid bacteria</subject><subject>Lactobacilli</subject><subject>Lactobacillus</subject><subject>Lactobacillus - drug effects</subject><subject>Lactobacillus - isolation & purification</subject><subject>Lactobacillus - metabolism</subject><subject>Lactobacillus - physiology</subject><subject>Lactobacillus acidophilus</subject><subject>Lactobacillus plantarum</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Low density lipoproteins</subject><subject>Male</subject><subject>Milk</subject><subject>Minimum inhibitory concentration</subject><subject>Precipitation (Meteorology)</subject><subject>Probiotics</subject><subject>Probiotics - metabolism</subject><subject>Probiotics - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Ribosomal DNA</subject><subject>Rodents</subject><subject>Salt</subject><subject>Salts</subject><subject>Small intestine</subject><subject>Species Specificity</subject><subject>Starter cultures</subject><subject>Strains (organisms)</subject><subject>Tibet</subject><subject>Vancomycin</subject><subject>Yogurt</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLgujFjEnzfSMsix8DA7vo6m1I8zGTMdOMSSv6783sdJep7IUU2pA-5z15T96qeg7BHCIG323ikDoV5rvY2TkAVHDKH1SnUKBmRhuAHh6tT6onOW8AIIhT-rg6aRAXmBB8Wl1dpdj62Htd71Lc2dR7m-vo6qXSfWyV9iEMuc59Ur7Ltc8xqN6a2qW4ra99a3vV1T-s86le3SBPq0dOhWyfjd-z6tvHD9cXn2fLy0-Li_PlTFOG-MxQQzXkjGFnEKGYGcFZeVsrAAGYKk4INEwjTKA23ImGAswEVEY4TKlCZ9XLg-4uxCzHYWQJccMQA8VfIRYHwkS1kbvktyr9kVF5ebMR00qqYlcHKylnjWgBY9w0uMGtskUCOd0IoynRpmi9H7sN7dYabbsykDARnf7p_Fqu4i9ZboozgYvAm1EgxZ-Dzb3c-qxtCKqzcdifG5LikHNS0Ff_oPe7G6mVKgZ852Lpq_ei8hwzDikDEBVqfg9VHmO3XpfkOF_2JwVvJwWF6e3vfqWGnOXi65f_Zy-_T9nXR-zaqtCvS5aG3scuT0F8AHWKOSfr7oYMgdwH_3Yach98OQa_lL04vqC7otuko7-x7fyG</recordid><startdate>20130722</startdate><enddate>20130722</enddate><creator>Zheng, Yongchen</creator><creator>Lu, Yingli</creator><creator>Wang, Jinfeng</creator><creator>Yang, Longfei</creator><creator>Pan, Chenyu</creator><creator>Huang, Ying</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130722</creationdate><title>Probiotic properties of Lactobacillus strains isolated from Tibetan kefir grains</title><author>Zheng, Yongchen ; Lu, Yingli ; Wang, Jinfeng ; Yang, Longfei ; Pan, Chenyu ; Huang, Ying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6738-d6d6c18774fd35647d98747dee905046a8551d7c3451cd8f92604791ad9f466a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acid resistance</topic><topic>Acids</topic><topic>Analysis</topic><topic>Animals</topic><topic>Anti-Bacterial Agents - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zheng, Yongchen</au><au>Lu, Yingli</au><au>Wang, Jinfeng</au><au>Yang, Longfei</au><au>Pan, Chenyu</au><au>Huang, Ying</au><au>Lobaccaro, Jean-Marc A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Probiotic properties of Lactobacillus strains isolated from Tibetan kefir grains</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-07-22</date><risdate>2013</risdate><volume>8</volume><issue>7</issue><spage>e69868</spage><epage>e69868</epage><pages>e69868-e69868</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The objective of this study was to evaluate the functional properties of lactic acid bacteria (LAB) isolated from Tibetan kefir grains. Three Lactobacillus isolates identified as Lactobacillus acidophilus LA15, Lactobacillus plantarum B23 and Lactobacillus kefiri D17 that showed resistance to acid and bile salts were selected for further evaluation of their probiotic properties. The 3 selected strains expressed high in vitro adherence to Caco-2 cells. They were sensitive to gentamicin, erythromycin and chloramphenicol and resistant to vancomycin with MIC values of 26 µg/ml. All 3 strains showed potential bile salt hydrolase (BSH) activity, cholesterol assimilation and cholesterol co-precipitation ability. Additionally, the potential effect of these strains on plasma cholesterol levels was evaluated in Sprague-Dawley (SD) rats. Rats in 4 treatment groups were fed the following experimental diets for 4 weeks: a high-cholesterol diet, a high-cholesterol diet plus LA15, a high-cholesterol diet plus B23 or a high-cholesterol diet plus D17. The total cholesterol, triglyceride and low-density lipoprotein cholesterol levels in the serum were significantly (P<0.05) decreased in the LAB-treated rats compared with rats fed a high-cholesterol diet without LAB supplementation. The high-density lipoprotein cholesterol levels in groups B23 and D17 were significantly (P<0.05) higher than those in the control and LA15 groups. Additionally, both fecal cholesterol and bile acid levels were significantly (P<0.05) increased after LAB administration. Fecal lactobacilli counts were significantly (P<0.05) higher in the LAB treatment groups than in the control groups. Furthermore, the 3 strains were detected in the rat small intestine, colon and feces during the feeding trial. The bacteria levels remained high even after the LAB administration had been stopped for 2 weeks. These results suggest that these strains may be used in the future as probiotic starter cultures for manufacturing novel fermented foods.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23894554</pmid><doi>10.1371/journal.pone.0069868</doi><tpages>e69868</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-07, Vol.8 (7), p.e69868-e69868 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1427370389 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acid resistance Acids Analysis Animals Anti-Bacterial Agents - pharmacology Antibiotics Bacteria Bacterial Adhesion Bile Bile acids Bile Acids and Salts - metabolism Bile salts Biology Body Weight - drug effects Caco-2 Cells Chloramphenicol Cholesterol Cholesterol - blood Cholesterol - metabolism Colon Cultured Milk Products - microbiology Dietary supplements Drug resistance Drug Resistance, Bacterial Eating - drug effects Edible Grain - microbiology Erythromycin Feces - microbiology Fermented food Gentamicin Grains High cholesterol diet Humans Hydrogen-Ion Concentration Hydrolase Hypolipidemic Agents - metabolism Hypolipidemic Agents - pharmacology Intestines - microbiology Kefir Laboratories Lactic acid Lactic acid bacteria Lactobacilli Lactobacillus Lactobacillus - drug effects Lactobacillus - isolation & purification Lactobacillus - metabolism Lactobacillus - physiology Lactobacillus acidophilus Lactobacillus plantarum Liver - drug effects Liver - metabolism Low density lipoproteins Male Milk Minimum inhibitory concentration Precipitation (Meteorology) Probiotics Probiotics - metabolism Probiotics - pharmacology Rats Rats, Sprague-Dawley Ribosomal DNA Rodents Salt Salts Small intestine Species Specificity Starter cultures Strains (organisms) Tibet Vancomycin Yogurt |
| title | Probiotic properties of Lactobacillus strains isolated from Tibetan kefir grains |
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