Assessing the fecal microbiota: an optimized ion torrent 16S rRNA gene-based analysis protocol

Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of nove...

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Veröffentlicht in:	PloS one 2013-07, Vol.8 (7), p.e68739-e68739
Hauptverfasser:	Milani, Christian, Hevia, Arancha, Foroni, Elena, Duranti, Sabrina, Turroni, Francesca, Lugli, Gabriele Andrea, Sanchez, Borja, Martín, Rebeca, Gueimonde, Miguel, van Sinderen, Douwe, Margolles, Abelardo, Ventura, Marco
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animals Bacteria Biodiversity Bioinformatics Biological samples Biology Deoxyribonucleic acid DNA DNA sequencing Fecal microflora Feces Feces - microbiology Female Gastrointestinal diseases Gene sequencing Genes Health care Humans Intestinal microflora Laboratories Life Sciences Microbiota Microbiota (Symbiotic organisms) Microorganisms Nucleotide sequencing Phylogenetics Primers Rats RNA RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA - methods
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