Antiviral responses by Swine primary bronchoepithelial cells are limited compared to human bronchoepithelial cells following influenza virus infection
Swine generate reassortant influenza viruses because they can be simultaneously infected with avian and human influenza; however, the features that restrict influenza reassortment in swine and human hosts are not fully understood. Type I and III interferons (IFNs) act as the first line of defense ag...
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| creator | Hauser, Mary J Dlugolenski, Daniel Culhane, Marie R Wentworth, David E Tompkins, S Mark Tripp, Ralph A |
| description | Swine generate reassortant influenza viruses because they can be simultaneously infected with avian and human influenza; however, the features that restrict influenza reassortment in swine and human hosts are not fully understood. Type I and III interferons (IFNs) act as the first line of defense against influenza virus infection of respiratory epithelium. To determine if human and swine have different capacities to mount an antiviral response the expression of IFN and IFN-stimulated genes (ISG) in normal human bronchial epithelial (NHBE) cells and normal swine bronchial epithelial (NSBE) cells was evaluated following infection with human (H3N2), swine (H1N1), and avian (H5N3, H5N2, H5N1) influenza A viruses. Expression of IFNλ and ISGs were substantially higher in NHBE cells compared to NSBE cells following H5 avian influenza virus infection compared to human or swine influenza virus infection. This effect was associated with reduced H5 avian influenza virus replication in human cells at late times post infection. Further, RIG-I expression was lower in NSBE cells compared to NHBE cells suggesting reduced virus sensing. Together, these studies identify key differences in the antiviral response between human and swine respiratory epithelium alluding to differences that may govern influenza reassortment. |
| doi_str_mv | 10.1371/journal.pone.0070251 |
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Type I and III interferons (IFNs) act as the first line of defense against influenza virus infection of respiratory epithelium. To determine if human and swine have different capacities to mount an antiviral response the expression of IFN and IFN-stimulated genes (ISG) in normal human bronchial epithelial (NHBE) cells and normal swine bronchial epithelial (NSBE) cells was evaluated following infection with human (H3N2), swine (H1N1), and avian (H5N3, H5N2, H5N1) influenza A viruses. Expression of IFNλ and ISGs were substantially higher in NHBE cells compared to NSBE cells following H5 avian influenza virus infection compared to human or swine influenza virus infection. This effect was associated with reduced H5 avian influenza virus replication in human cells at late times post infection. Further, RIG-I expression was lower in NSBE cells compared to NHBE cells suggesting reduced virus sensing. 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Type I and III interferons (IFNs) act as the first line of defense against influenza virus infection of respiratory epithelium. To determine if human and swine have different capacities to mount an antiviral response the expression of IFN and IFN-stimulated genes (ISG) in normal human bronchial epithelial (NHBE) cells and normal swine bronchial epithelial (NSBE) cells was evaluated following infection with human (H3N2), swine (H1N1), and avian (H5N3, H5N2, H5N1) influenza A viruses. Expression of IFNλ and ISGs were substantially higher in NHBE cells compared to NSBE cells following H5 avian influenza virus infection compared to human or swine influenza virus infection. This effect was associated with reduced H5 avian influenza virus replication in human cells at late times post infection. Further, RIG-I expression was lower in NSBE cells compared to NHBE cells suggesting reduced virus sensing. Together, these studies identify key differences in the antiviral response between human and swine respiratory epithelium alluding to differences that may govern influenza reassortment.</description><subject>Acids</subject><subject>Adolescent</subject><subject>Animals</subject><subject>Antiviral drugs</subject><subject>Avian flu</subject><subject>Avian influenza</subject><subject>Avian influenza viruses</subject><subject>Bronchi - immunology</subject><subject>Bronchi - virology</subject><subject>Cells (Biology)</subject><subject>Cells, Cultured</subject><subject>Cytokines</subject><subject>Epithelial Cells - immunology</subject><subject>Epithelial Cells - virology</subject><subject>Epithelium</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genomes</subject><subject>Human behavior</subject><subject>Humans</subject><subject>Immunity, Cellular - physiology</subject><subject>Infection</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza, Human - 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Type I and III interferons (IFNs) act as the first line of defense against influenza virus infection of respiratory epithelium. To determine if human and swine have different capacities to mount an antiviral response the expression of IFN and IFN-stimulated genes (ISG) in normal human bronchial epithelial (NHBE) cells and normal swine bronchial epithelial (NSBE) cells was evaluated following infection with human (H3N2), swine (H1N1), and avian (H5N3, H5N2, H5N1) influenza A viruses. Expression of IFNλ and ISGs were substantially higher in NHBE cells compared to NSBE cells following H5 avian influenza virus infection compared to human or swine influenza virus infection. This effect was associated with reduced H5 avian influenza virus replication in human cells at late times post infection. Further, RIG-I expression was lower in NSBE cells compared to NHBE cells suggesting reduced virus sensing. Together, these studies identify key differences in the antiviral response between human and swine respiratory epithelium alluding to differences that may govern influenza reassortment.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23875024</pmid><doi>10.1371/journal.pone.0070251</doi><tpages>e70251</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Adolescent Animals Antiviral drugs Avian flu Avian influenza Avian influenza viruses Bronchi - immunology Bronchi - virology Cells (Biology) Cells, Cultured Cytokines Epithelial Cells - immunology Epithelial Cells - virology Epithelium Gene expression Genes Genomes Human behavior Humans Immunity, Cellular - physiology Infection Infections Infectious diseases Influenza Influenza A Influenza, Human - immunology Interferon Livestock Male Orthomyxoviridae - immunology Orthomyxoviridae Infections - immunology Pandemics Primary Cell Culture Proteins Respiratory Mucosa - immunology Respiratory tract Swine Swine Diseases - immunology Swine flu Swine influenza Veterinary colleges Veterinary medicine Viral infections Virus diseases Virus replication Viruses |
| title | Antiviral responses by Swine primary bronchoepithelial cells are limited compared to human bronchoepithelial cells following influenza virus infection |
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