The dynamics of connexin expression, degradation and localisation are regulated by gonadotropins during the early stages of in vitro maturation of swine oocytes
Gap junctional communication (GJC) plays a primordial role in oocyte maturation and meiotic resumption in mammals by directing the transfer of numerous molecules between cumulus cells and the oocyte. Gap junctions are made of connexins (Cx), proteins that regulate GJC in numerous ways. Understanding...
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| description | Gap junctional communication (GJC) plays a primordial role in oocyte maturation and meiotic resumption in mammals by directing the transfer of numerous molecules between cumulus cells and the oocyte. Gap junctions are made of connexins (Cx), proteins that regulate GJC in numerous ways. Understanding the dynamic regulation of connexin arrangements during in vitro maturation (IVM) could provide a powerful tool for controlling meiotic resumption and consequently in vitro development of fully competent oocytes. However, physiological events happening during the early hours of IVM may still be elucidated. The present study reports the dynamic regulation of connexin expression, degradation and localization during this stage. Cx43, Cx45 and Cx60 were identified as the main connexins expressed in swine COC. Cx43 and Cx45 transcripts were judged too static to be a regulator of GJC, while Cx43 protein expression was highly responsive to gonadotropins, suggesting that it might be the principal regulator of GJC. In addition, the degradation of Cx43 expressed after 4.5 h of IVM in response to equine chorionic gonadotropin appeared to involve the proteasomal complex. Cx43 localisation appeared to be associated with GJC. Taken together, these results show for the first time that gonadotropins regulate Cx43 protein expression, degradation and localisation in porcine COC during the first several hours of IVM. Regulation of Cx43 may in turn, via GJC, participate in the development of fully competent oocytes. |
| doi_str_mv | 10.1371/journal.pone.0068456 |
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Gap junctions are made of connexins (Cx), proteins that regulate GJC in numerous ways. Understanding the dynamic regulation of connexin arrangements during in vitro maturation (IVM) could provide a powerful tool for controlling meiotic resumption and consequently in vitro development of fully competent oocytes. However, physiological events happening during the early hours of IVM may still be elucidated. The present study reports the dynamic regulation of connexin expression, degradation and localization during this stage. Cx43, Cx45 and Cx60 were identified as the main connexins expressed in swine COC. Cx43 and Cx45 transcripts were judged too static to be a regulator of GJC, while Cx43 protein expression was highly responsive to gonadotropins, suggesting that it might be the principal regulator of GJC. In addition, the degradation of Cx43 expressed after 4.5 h of IVM in response to equine chorionic gonadotropin appeared to involve the proteasomal complex. Cx43 localisation appeared to be associated with GJC. Taken together, these results show for the first time that gonadotropins regulate Cx43 protein expression, degradation and localisation in porcine COC during the first several hours of IVM. Regulation of Cx43 may in turn, via GJC, participate in the development of fully competent oocytes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0068456</identifier><identifier>PMID: 23861906</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Apoptosis ; Biology ; Cancer ; Cell Communication - drug effects ; Cell cycle ; Cells, Cultured ; Chorionic gonadotropin ; Communication ; Connexin 43 ; Connexin 43 - genetics ; Connexin 43 - metabolism ; Connexin 45 ; Connexins ; Connexins - genetics ; Connexins - metabolism ; Cumulus Cells - cytology ; Cumulus Cells - drug effects ; Cumulus Cells - metabolism ; Degradation ; Female ; Gap junctions ; Gap Junctions - drug effects ; Gap Junctions - metabolism ; Gene expression ; Gene Expression Regulation, Developmental ; Gonadotropins ; Gonadotropins, Equine - metabolism ; Gonadotropins, Equine - pharmacology ; Horses ; Livestock ; Localization ; Mammals ; Maturation ; Medical research ; Medicine ; Meiosis ; Oocytes ; Oocytes - cytology ; Oocytes - drug effects ; Oocytes - metabolism ; Oogenesis - drug effects ; Oogenesis - genetics ; Pituitary (anterior) ; Polymerase chain reaction ; Proteasomes ; Protein Stability - drug effects ; Proteins ; Proteolysis - drug effects ; Recovery (Medical) ; Rodents ; Signal Transduction ; Swine</subject><ispartof>PloS one, 2013-07, Vol.8 (7), p.e68456</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Santiquet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Santiquet et al 2013 Santiquet et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-7e58cc232f5c57670e27108880fd8d1e5b4e0ca5503cb9338dc5506a86c918d83</citedby><cites>FETCH-LOGICAL-c692t-7e58cc232f5c57670e27108880fd8d1e5b4e0ca5503cb9338dc5506a86c918d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701662/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3701662/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23861906$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yan, Wei</contributor><creatorcontrib>Santiquet, Nicolas</creatorcontrib><creatorcontrib>Robert, Claude</creatorcontrib><creatorcontrib>Richard, François J</creatorcontrib><title>The dynamics of connexin expression, degradation and localisation are regulated by gonadotropins during the early stages of in vitro maturation of swine oocytes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Gap junctional communication (GJC) plays a primordial role in oocyte maturation and meiotic resumption in mammals by directing the transfer of numerous molecules between cumulus cells and the oocyte. 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Cx43 localisation appeared to be associated with GJC. Taken together, these results show for the first time that gonadotropins regulate Cx43 protein expression, degradation and localisation in porcine COC during the first several hours of IVM. Regulation of Cx43 may in turn, via GJC, participate in the development of fully competent oocytes.</description><subject>Analysis</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biology</subject><subject>Cancer</subject><subject>Cell Communication - drug effects</subject><subject>Cell cycle</subject><subject>Cells, Cultured</subject><subject>Chorionic gonadotropin</subject><subject>Communication</subject><subject>Connexin 43</subject><subject>Connexin 43 - genetics</subject><subject>Connexin 43 - metabolism</subject><subject>Connexin 45</subject><subject>Connexins</subject><subject>Connexins - genetics</subject><subject>Connexins - metabolism</subject><subject>Cumulus Cells - cytology</subject><subject>Cumulus Cells - drug effects</subject><subject>Cumulus Cells - metabolism</subject><subject>Degradation</subject><subject>Female</subject><subject>Gap junctions</subject><subject>Gap Junctions - drug effects</subject><subject>Gap Junctions - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gonadotropins</subject><subject>Gonadotropins, Equine - 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drug effects</topic><topic>Cell cycle</topic><topic>Cells, Cultured</topic><topic>Chorionic gonadotropin</topic><topic>Communication</topic><topic>Connexin 43</topic><topic>Connexin 43 - genetics</topic><topic>Connexin 43 - metabolism</topic><topic>Connexin 45</topic><topic>Connexins</topic><topic>Connexins - genetics</topic><topic>Connexins - metabolism</topic><topic>Cumulus Cells - cytology</topic><topic>Cumulus Cells - drug effects</topic><topic>Cumulus Cells - metabolism</topic><topic>Degradation</topic><topic>Female</topic><topic>Gap junctions</topic><topic>Gap Junctions - drug effects</topic><topic>Gap Junctions - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gonadotropins</topic><topic>Gonadotropins, Equine - metabolism</topic><topic>Gonadotropins, Equine - pharmacology</topic><topic>Horses</topic><topic>Livestock</topic><topic>Localization</topic><topic>Mammals</topic><topic>Maturation</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Meiosis</topic><topic>Oocytes</topic><topic>Oocytes - 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Gap junctions are made of connexins (Cx), proteins that regulate GJC in numerous ways. Understanding the dynamic regulation of connexin arrangements during in vitro maturation (IVM) could provide a powerful tool for controlling meiotic resumption and consequently in vitro development of fully competent oocytes. However, physiological events happening during the early hours of IVM may still be elucidated. The present study reports the dynamic regulation of connexin expression, degradation and localization during this stage. Cx43, Cx45 and Cx60 were identified as the main connexins expressed in swine COC. Cx43 and Cx45 transcripts were judged too static to be a regulator of GJC, while Cx43 protein expression was highly responsive to gonadotropins, suggesting that it might be the principal regulator of GJC. In addition, the degradation of Cx43 expressed after 4.5 h of IVM in response to equine chorionic gonadotropin appeared to involve the proteasomal complex. Cx43 localisation appeared to be associated with GJC. Taken together, these results show for the first time that gonadotropins regulate Cx43 protein expression, degradation and localisation in porcine COC during the first several hours of IVM. Regulation of Cx43 may in turn, via GJC, participate in the development of fully competent oocytes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23861906</pmid><doi>10.1371/journal.pone.0068456</doi><tpages>e68456</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-07, Vol.8 (7), p.e68456 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Apoptosis Biology Cancer Cell Communication - drug effects Cell cycle Cells, Cultured Chorionic gonadotropin Communication Connexin 43 Connexin 43 - genetics Connexin 43 - metabolism Connexin 45 Connexins Connexins - genetics Connexins - metabolism Cumulus Cells - cytology Cumulus Cells - drug effects Cumulus Cells - metabolism Degradation Female Gap junctions Gap Junctions - drug effects Gap Junctions - metabolism Gene expression Gene Expression Regulation, Developmental Gonadotropins Gonadotropins, Equine - metabolism Gonadotropins, Equine - pharmacology Horses Livestock Localization Mammals Maturation Medical research Medicine Meiosis Oocytes Oocytes - cytology Oocytes - drug effects Oocytes - metabolism Oogenesis - drug effects Oogenesis - genetics Pituitary (anterior) Polymerase chain reaction Proteasomes Protein Stability - drug effects Proteins Proteolysis - drug effects Recovery (Medical) Rodents Signal Transduction Swine |
| title | The dynamics of connexin expression, degradation and localisation are regulated by gonadotropins during the early stages of in vitro maturation of swine oocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T04%3A15%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20dynamics%20of%20connexin%20expression,%20degradation%20and%20localisation%20are%20regulated%20by%20gonadotropins%20during%20the%20early%20stages%20of%20in%20vitro%20maturation%20of%20swine%20oocytes&rft.jtitle=PloS%20one&rft.au=Santiquet,%20Nicolas&rft.date=2013-07-04&rft.volume=8&rft.issue=7&rft.spage=e68456&rft.pages=e68456-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0068456&rft_dat=%3Cgale_plos_%3EA478428867%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1394489219&rft_id=info:pmid/23861906&rft_galeid=A478428867&rft_doaj_id=oai_doaj_org_article_23abf2b9854f4827901ddb3c09001ea7&rfr_iscdi=true |