Hesperetin alleviates the inhibitory effects of high glucose on the osteoblastic differentiation of periodontal ligament stem cells
Hesperetin (3',5,7-trihydroxy-4-methoxyflavanone) is a metabolite of hesperidin (hesperetin-7-O-rutinoside), which belongs to the flavanone subgroup and is found mainly in citrus fruits. Hesperetin has been reported to be an effective osteoinductive compound in various in vivo and in vitro mode...
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| description | Hesperetin (3',5,7-trihydroxy-4-methoxyflavanone) is a metabolite of hesperidin (hesperetin-7-O-rutinoside), which belongs to the flavanone subgroup and is found mainly in citrus fruits. Hesperetin has been reported to be an effective osteoinductive compound in various in vivo and in vitro models. However, how hesperetin effects osteogenic differentiation is not fully understood. In this study, we investigated the capacity of hesperetin to stimulate the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and to relieve the anti-osteogenic effect of high glucose. Osteogenesis of PDLSCs was assessed by measurement of alkaline phosphatase (ALP) activity, and evaluation of the mRNA expression of ALP, runt-related gene 2 (Runx2), osterix (OSX), and FRA1 as osteogenic transcription factors, as well as assessment of protein expression of osteopontin (OPN) and collagen type IA (COLIA). When PDLSCs were exposed to a high concentration (30 mM) of glucose, osteogenic activity decreased compared to control cells. Hesperetin significantly increased ALP activity at doses of 1, 10, and 100 µM. Pretreatment of cells with hesperetin alleviated the high-glucose-induced suppression of the osteogenic activity of PDLSCs. Hesperetin scavenged intracellular reactive oxygen species (ROS) produced under high glucose condition. Furthermore, hesperetin increased the activity of the PI3K/Akt and β-catenin pathways. Consistent with this, blockage of Akt or β-catenin diminished the protective effect of hesperetin against high glucose-inhibited osteogenic differentiation. Collectively, our results suggest that hesperetin alleviates the high glucose-mediated suppression of osteogenic differentiation in PDLSCs by regulating ROS levels and the PI3K/Akt and β-catenin signaling pathways. |
| doi_str_mv | 10.1371/journal.pone.0067504 |
| format | Article |
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Hesperetin has been reported to be an effective osteoinductive compound in various in vivo and in vitro models. However, how hesperetin effects osteogenic differentiation is not fully understood. In this study, we investigated the capacity of hesperetin to stimulate the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and to relieve the anti-osteogenic effect of high glucose. Osteogenesis of PDLSCs was assessed by measurement of alkaline phosphatase (ALP) activity, and evaluation of the mRNA expression of ALP, runt-related gene 2 (Runx2), osterix (OSX), and FRA1 as osteogenic transcription factors, as well as assessment of protein expression of osteopontin (OPN) and collagen type IA (COLIA). When PDLSCs were exposed to a high concentration (30 mM) of glucose, osteogenic activity decreased compared to control cells. Hesperetin significantly increased ALP activity at doses of 1, 10, and 100 µM. Pretreatment of cells with hesperetin alleviated the high-glucose-induced suppression of the osteogenic activity of PDLSCs. Hesperetin scavenged intracellular reactive oxygen species (ROS) produced under high glucose condition. Furthermore, hesperetin increased the activity of the PI3K/Akt and β-catenin pathways. Consistent with this, blockage of Akt or β-catenin diminished the protective effect of hesperetin against high glucose-inhibited osteogenic differentiation. Collectively, our results suggest that hesperetin alleviates the high glucose-mediated suppression of osteogenic differentiation in PDLSCs by regulating ROS levels and the PI3K/Akt and β-catenin signaling pathways.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0067504</identifier><identifier>PMID: 23840726</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Alkaline phosphatase ; Alkaline Phosphatase - genetics ; Alkaline Phosphatase - metabolism ; beta Catenin - genetics ; beta Catenin - metabolism ; Biocompatibility ; Biology ; Biomedical engineering ; Biomedical materials ; Blockage ; Cbfa-1 protein ; Cell Differentiation - drug effects ; Cell Differentiation - genetics ; Cells, Cultured ; Citrus fruits ; Collagen ; Dentistry ; Diabetes ; Differentiation ; Flavonoids ; Fra1 protein ; Fruits ; Gene expression ; Gene Expression - drug effects ; Gene Expression - genetics ; Glucose ; Glucose - metabolism ; Hesperetin ; Hesperidin ; Hesperidin - pharmacology ; Humans ; Kinases ; Ligaments ; Metabolites ; Osteoblastogenesis ; Osteoblasts ; Osteoblasts - drug effects ; Osteoblasts - metabolism ; Osteogenesis ; Osteogenesis - drug effects ; Osteogenesis - genetics ; Osteopontin ; Oxidative stress ; Oxygen ; Pathways ; Periodontal ligament ; Periodontal Ligament - drug effects ; Periodontal Ligament - metabolism ; Phosphatase ; Phosphatidylinositol 3-Kinases - genetics ; Phosphatidylinositol 3-Kinases - metabolism ; Pretreatment ; Proteins ; Proto-Oncogene Proteins c-akt - genetics ; Proto-Oncogene Proteins c-akt - metabolism ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Signal Transduction - drug effects ; Signal Transduction - genetics ; Signaling ; Stem cells ; Stem Cells - drug effects ; Stem Cells - metabolism ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; β-Catenin</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e67504</ispartof><rights>2013 Kim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Kim et al 2013 Kim et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-f70c515325d6797004561534d4b28f190cc967b10da8daf74c0fa52f802ac343</citedby><cites>FETCH-LOGICAL-c592t-f70c515325d6797004561534d4b28f190cc967b10da8daf74c0fa52f802ac343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696082/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3696082/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23840726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, So Yeon</creatorcontrib><creatorcontrib>Lee, Jin-Yong</creatorcontrib><creatorcontrib>Park, Yong-Duk</creatorcontrib><creatorcontrib>Kang, Kyung Lhi</creatorcontrib><creatorcontrib>Lee, Jeong-Chae</creatorcontrib><creatorcontrib>Heo, Jung Sun</creatorcontrib><title>Hesperetin alleviates the inhibitory effects of high glucose on the osteoblastic differentiation of periodontal ligament stem cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Hesperetin (3',5,7-trihydroxy-4-methoxyflavanone) is a metabolite of hesperidin (hesperetin-7-O-rutinoside), which belongs to the flavanone subgroup and is found mainly in citrus fruits. Hesperetin has been reported to be an effective osteoinductive compound in various in vivo and in vitro models. However, how hesperetin effects osteogenic differentiation is not fully understood. In this study, we investigated the capacity of hesperetin to stimulate the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and to relieve the anti-osteogenic effect of high glucose. Osteogenesis of PDLSCs was assessed by measurement of alkaline phosphatase (ALP) activity, and evaluation of the mRNA expression of ALP, runt-related gene 2 (Runx2), osterix (OSX), and FRA1 as osteogenic transcription factors, as well as assessment of protein expression of osteopontin (OPN) and collagen type IA (COLIA). When PDLSCs were exposed to a high concentration (30 mM) of glucose, osteogenic activity decreased compared to control cells. Hesperetin significantly increased ALP activity at doses of 1, 10, and 100 µM. Pretreatment of cells with hesperetin alleviated the high-glucose-induced suppression of the osteogenic activity of PDLSCs. Hesperetin scavenged intracellular reactive oxygen species (ROS) produced under high glucose condition. Furthermore, hesperetin increased the activity of the PI3K/Akt and β-catenin pathways. Consistent with this, blockage of Akt or β-catenin diminished the protective effect of hesperetin against high glucose-inhibited osteogenic differentiation. Collectively, our results suggest that hesperetin alleviates the high glucose-mediated suppression of osteogenic differentiation in PDLSCs by regulating ROS levels and the PI3K/Akt and β-catenin signaling pathways.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Alkaline phosphatase</subject><subject>Alkaline Phosphatase - genetics</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>beta Catenin - genetics</subject><subject>beta Catenin - metabolism</subject><subject>Biocompatibility</subject><subject>Biology</subject><subject>Biomedical engineering</subject><subject>Biomedical materials</subject><subject>Blockage</subject><subject>Cbfa-1 protein</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - genetics</subject><subject>Cells, Cultured</subject><subject>Citrus fruits</subject><subject>Collagen</subject><subject>Dentistry</subject><subject>Diabetes</subject><subject>Differentiation</subject><subject>Flavonoids</subject><subject>Fra1 protein</subject><subject>Fruits</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression - genetics</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Hesperetin</subject><subject>Hesperidin</subject><subject>Hesperidin - pharmacology</subject><subject>Humans</subject><subject>Kinases</subject><subject>Ligaments</subject><subject>Metabolites</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - metabolism</subject><subject>Osteogenesis</subject><subject>Osteogenesis - drug effects</subject><subject>Osteogenesis - genetics</subject><subject>Osteopontin</subject><subject>Oxidative stress</subject><subject>Oxygen</subject><subject>Pathways</subject><subject>Periodontal ligament</subject><subject>Periodontal Ligament - drug effects</subject><subject>Periodontal Ligament - metabolism</subject><subject>Phosphatase</subject><subject>Phosphatidylinositol 3-Kinases - genetics</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Pretreatment</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-akt - genetics</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - genetics</subject><subject>Signaling</subject><subject>Stem cells</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>β-Catenin</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNp1ksluFDEQhlsIRELgDRBY4jxDeWm7-4KEIrJIkbjkbrnd5R6PPO3B9kTKmRfHyXSi5MDJS33_X4uqaT5TWFOu6PdtPKTZhPU-zrgGkKoF8aY5pT1nK8mAv31xP2k-5LwFaHkn5fvmhPFOgGLytPl7hXmPCYufiQkB77wpmEnZIPHzxg--xHRP0Dm0JZPoyMZPGzKFg40ZSZwfyZgLxiGYXLwlo69wwrlUJ1-BqqkJfBzjXEwgwU9mV6OkanbEYgj5Y_POmZDx03KeNbcXv27Pr1Y3vy-vz3_erGzbs7JyCmxLW87aUapeAYhW1qcYxcA6R3uwtpdqoDCabjROCQvOtMx1wIzlgp81X4-2-xCzXsaXdR0m4y0oAZW4PhJjNFu9T35n0r2OxuvHj5gmbVLtMaCWcjR0pNYpI0Sn-MDAMeeEQseRGlq9fizZDsMOR1tbTia8Mn0dmf1GT_FOc9lL6Fg1-LYYpPjngLn8p2RxpGyKOSd0zxkoPHD0SaUf9kQve1JlX15W9yx6Wgz-D1iTvyk</recordid><startdate>20130628</startdate><enddate>20130628</enddate><creator>Kim, So Yeon</creator><creator>Lee, Jin-Yong</creator><creator>Park, Yong-Duk</creator><creator>Kang, Kyung Lhi</creator><creator>Lee, Jeong-Chae</creator><creator>Heo, Jung Sun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130628</creationdate><title>Hesperetin alleviates the inhibitory effects of high glucose on the osteoblastic differentiation of periodontal ligament stem cells</title><author>Kim, So Yeon ; Lee, Jin-Yong ; Park, Yong-Duk ; Kang, Kyung Lhi ; Lee, Jeong-Chae ; Heo, Jung Sun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-f70c515325d6797004561534d4b28f190cc967b10da8daf74c0fa52f802ac343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Alkaline phosphatase</topic><topic>Alkaline Phosphatase - genetics</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>beta Catenin - genetics</topic><topic>beta Catenin - metabolism</topic><topic>Biocompatibility</topic><topic>Biology</topic><topic>Biomedical engineering</topic><topic>Biomedical materials</topic><topic>Blockage</topic><topic>Cbfa-1 protein</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - genetics</topic><topic>Cells, Cultured</topic><topic>Citrus fruits</topic><topic>Collagen</topic><topic>Dentistry</topic><topic>Diabetes</topic><topic>Differentiation</topic><topic>Flavonoids</topic><topic>Fra1 protein</topic><topic>Fruits</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>Gene Expression - genetics</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>Hesperetin</topic><topic>Hesperidin</topic><topic>Hesperidin - pharmacology</topic><topic>Humans</topic><topic>Kinases</topic><topic>Ligaments</topic><topic>Metabolites</topic><topic>Osteoblastogenesis</topic><topic>Osteoblasts</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - metabolism</topic><topic>Osteogenesis</topic><topic>Osteogenesis - drug effects</topic><topic>Osteogenesis - genetics</topic><topic>Osteopontin</topic><topic>Oxidative stress</topic><topic>Oxygen</topic><topic>Pathways</topic><topic>Periodontal ligament</topic><topic>Periodontal Ligament - drug effects</topic><topic>Periodontal Ligament - metabolism</topic><topic>Phosphatase</topic><topic>Phosphatidylinositol 3-Kinases - genetics</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Pretreatment</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-akt - genetics</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - genetics</topic><topic>Signaling</topic><topic>Stem cells</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>β-Catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, So Yeon</creatorcontrib><creatorcontrib>Lee, Jin-Yong</creatorcontrib><creatorcontrib>Park, Yong-Duk</creatorcontrib><creatorcontrib>Kang, Kyung Lhi</creatorcontrib><creatorcontrib>Lee, Jeong-Chae</creatorcontrib><creatorcontrib>Heo, Jung Sun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Hesperetin has been reported to be an effective osteoinductive compound in various in vivo and in vitro models. However, how hesperetin effects osteogenic differentiation is not fully understood. In this study, we investigated the capacity of hesperetin to stimulate the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and to relieve the anti-osteogenic effect of high glucose. Osteogenesis of PDLSCs was assessed by measurement of alkaline phosphatase (ALP) activity, and evaluation of the mRNA expression of ALP, runt-related gene 2 (Runx2), osterix (OSX), and FRA1 as osteogenic transcription factors, as well as assessment of protein expression of osteopontin (OPN) and collagen type IA (COLIA). When PDLSCs were exposed to a high concentration (30 mM) of glucose, osteogenic activity decreased compared to control cells. Hesperetin significantly increased ALP activity at doses of 1, 10, and 100 µM. Pretreatment of cells with hesperetin alleviated the high-glucose-induced suppression of the osteogenic activity of PDLSCs. Hesperetin scavenged intracellular reactive oxygen species (ROS) produced under high glucose condition. Furthermore, hesperetin increased the activity of the PI3K/Akt and β-catenin pathways. Consistent with this, blockage of Akt or β-catenin diminished the protective effect of hesperetin against high glucose-inhibited osteogenic differentiation. Collectively, our results suggest that hesperetin alleviates the high glucose-mediated suppression of osteogenic differentiation in PDLSCs by regulating ROS levels and the PI3K/Akt and β-catenin signaling pathways.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23840726</pmid><doi>10.1371/journal.pone.0067504</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2013-06, Vol.8 (6), p.e67504 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1372350740 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Alkaline phosphatase Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism beta Catenin - genetics beta Catenin - metabolism Biocompatibility Biology Biomedical engineering Biomedical materials Blockage Cbfa-1 protein Cell Differentiation - drug effects Cell Differentiation - genetics Cells, Cultured Citrus fruits Collagen Dentistry Diabetes Differentiation Flavonoids Fra1 protein Fruits Gene expression Gene Expression - drug effects Gene Expression - genetics Glucose Glucose - metabolism Hesperetin Hesperidin Hesperidin - pharmacology Humans Kinases Ligaments Metabolites Osteoblastogenesis Osteoblasts Osteoblasts - drug effects Osteoblasts - metabolism Osteogenesis Osteogenesis - drug effects Osteogenesis - genetics Osteopontin Oxidative stress Oxygen Pathways Periodontal ligament Periodontal Ligament - drug effects Periodontal Ligament - metabolism Phosphatase Phosphatidylinositol 3-Kinases - genetics Phosphatidylinositol 3-Kinases - metabolism Pretreatment Proteins Proto-Oncogene Proteins c-akt - genetics Proto-Oncogene Proteins c-akt - metabolism Reactive oxygen species Reactive Oxygen Species - metabolism Signal Transduction - drug effects Signal Transduction - genetics Signaling Stem cells Stem Cells - drug effects Stem Cells - metabolism Transcription factors Transcription Factors - genetics Transcription Factors - metabolism β-Catenin |
| title | Hesperetin alleviates the inhibitory effects of high glucose on the osteoblastic differentiation of periodontal ligament stem cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T10%3A51%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hesperetin%20alleviates%20the%20inhibitory%20effects%20of%20high%20glucose%20on%20the%20osteoblastic%20differentiation%20of%20periodontal%20ligament%20stem%20cells&rft.jtitle=PloS%20one&rft.au=Kim,%20So%20Yeon&rft.date=2013-06-28&rft.volume=8&rft.issue=6&rft.spage=e67504&rft.pages=e67504-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0067504&rft_dat=%3Cproquest_plos_%3E3007995731%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1372350740&rft_id=info:pmid/23840726&rft_doaj_id=oai_doaj_org_article_66da1d1cf7a44873b20f2ff47ef3e1a1&rfr_iscdi=true |