Application of D-Crustacean Hyperglycemic Hormone Induces Peptidases Transcription and Suppresses Glycolysis-Related Transcripts in the Hepatopancreas of the Crayfish Pontastacus leptodactylus - Results of a Transcriptomic Study
The crustacean Hyperglycemic Hormone (cHH) is a neuropeptide present in many decapods. Two different chiral isomers are simultaneously present in Astacid crayfish and their specific biological functions are still poorly understood. The present study is aimed at better understanding the potentially d...
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| description | The crustacean Hyperglycemic Hormone (cHH) is a neuropeptide present in many decapods. Two different chiral isomers are simultaneously present in Astacid crayfish and their specific biological functions are still poorly understood. The present study is aimed at better understanding the potentially different effect of each of the isomers on the hepatopancreatic gene expression profile in the crayfish Pontastacus leptodactylus, in the context of short term hyperglycemia. Hence, two different chemically synthesized cHH enantiomers, containing either L- or D-Phe(3), were injected to the circulation of intermolt females following removal of their X organ-Sinus gland complex. The effects triggered by the injection of the two alternate isomers were detected after one hour through measurement of circulating glucose levels. Triggered changes of the transcriptome expression profile in the hepatopancreas were analyzed by RNA-seq. A whole transcriptome shotgun sequence assembly provided the assumedly complete transcriptome of P. leptodactylus hepatopancreas, followed by RNA-seq analysis of changes in the expression level of many genes caused by the application of each of the hormone isomers. Circulating glucose levels were much higher in response to the D-isoform than to the L-isoform injection, one hour from injection. Similarly, the RNA-seq analysis confirmed a stronger effect on gene expression following the administration of D-cHH, while just limited alterations were caused by the L-isomer. These findings demonstrated a more prominent short term effect of the D-cHH on the transcription profile and shed light on the effect of the D-isomer on specific functional gene groups. Another contribution of the study is the construction of a de novo assembly of the hepatopancreas transcriptome, consisting of 39,935 contigs, that dramatically increases the molecular information available for this species and for crustaceans in general, providing an efficient tool for studying gene expression patterns in this organ. |
| doi_str_mv | 10.1371/journal.pone.0065176 |
| format | Article |
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Two different chiral isomers are simultaneously present in Astacid crayfish and their specific biological functions are still poorly understood. The present study is aimed at better understanding the potentially different effect of each of the isomers on the hepatopancreatic gene expression profile in the crayfish Pontastacus leptodactylus, in the context of short term hyperglycemia. Hence, two different chemically synthesized cHH enantiomers, containing either L- or D-Phe(3), were injected to the circulation of intermolt females following removal of their X organ-Sinus gland complex. The effects triggered by the injection of the two alternate isomers were detected after one hour through measurement of circulating glucose levels. Triggered changes of the transcriptome expression profile in the hepatopancreas were analyzed by RNA-seq. A whole transcriptome shotgun sequence assembly provided the assumedly complete transcriptome of P. leptodactylus hepatopancreas, followed by RNA-seq analysis of changes in the expression level of many genes caused by the application of each of the hormone isomers. Circulating glucose levels were much higher in response to the D-isoform than to the L-isoform injection, one hour from injection. Similarly, the RNA-seq analysis confirmed a stronger effect on gene expression following the administration of D-cHH, while just limited alterations were caused by the L-isomer. These findings demonstrated a more prominent short term effect of the D-cHH on the transcription profile and shed light on the effect of the D-isomer on specific functional gene groups. Another contribution of the study is the construction of a de novo assembly of the hepatopancreas transcriptome, consisting of 39,935 contigs, that dramatically increases the molecular information available for this species and for crustaceans in general, providing an efficient tool for studying gene expression patterns in this organ.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0065176</identifier><identifier>PMID: 23840318</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Arthropod Proteins - pharmacology ; Assembly ; Astacoidea - genetics ; Bioinformatics ; Biology ; Crayfish ; Crustaceans ; Decapoda ; Enantiomers ; Female ; Females ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Enzymologic - drug effects ; Genes ; Genetic engineering ; Genomes ; Genomics ; Glucose ; Glucose metabolism ; Glycolysis ; Glycolysis - drug effects ; Glycolysis - genetics ; Hepatopancreas ; Hepatopancreas - drug effects ; Hepatopancreas - enzymology ; Hepatopancreas - metabolism ; Hormones ; Hyperglycemia ; Injection ; Invertebrate Hormones - pharmacology ; Isomers ; Marsupenaeus japonicus ; Metabolic Networks and Pathways - drug effects ; Metabolic Networks and Pathways - genetics ; Metabolism ; Nerve Tissue Proteins - pharmacology ; Neuropeptides ; Nucleotide sequence ; Ontology ; Orconectes limosus ; Peptidases ; Peptide Hydrolases - genetics ; Peptides ; Periclimenaeus leptodactylus ; Physiology ; Procambarus clarkii ; Proteases ; Proteins ; Ribonucleic acid ; RNA ; Sinus ; Sinus gland ; Transcription ; Transcription (Genetics) ; Transcription, Genetic - drug effects ; Transcriptome - drug effects</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e65176</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Manfrin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Manfrin et al 2013 Manfrin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-bc6cbdb0f22a9a4a31533700864b577258a21c2f098b1a2e81bb61ac45f9c84e3</citedby><cites>FETCH-LOGICAL-c692t-bc6cbdb0f22a9a4a31533700864b577258a21c2f098b1a2e81bb61ac45f9c84e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686806/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3686806/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23840318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Buratti, Emanuele</contributor><creatorcontrib>Manfrin, Chiara</creatorcontrib><creatorcontrib>Tom, Moshe</creatorcontrib><creatorcontrib>De Moro, Gianluca</creatorcontrib><creatorcontrib>Gerdol, Marco</creatorcontrib><creatorcontrib>Guarnaccia, Corrado</creatorcontrib><creatorcontrib>Mosco, Alessandro</creatorcontrib><creatorcontrib>Pallavicini, Alberto</creatorcontrib><creatorcontrib>Giulianini, Piero Giulio</creatorcontrib><title>Application of D-Crustacean Hyperglycemic Hormone Induces Peptidases Transcription and Suppresses Glycolysis-Related Transcripts in the Hepatopancreas of the Crayfish Pontastacus leptodactylus - Results of a Transcriptomic Study</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The crustacean Hyperglycemic Hormone (cHH) is a neuropeptide present in many decapods. Two different chiral isomers are simultaneously present in Astacid crayfish and their specific biological functions are still poorly understood. The present study is aimed at better understanding the potentially different effect of each of the isomers on the hepatopancreatic gene expression profile in the crayfish Pontastacus leptodactylus, in the context of short term hyperglycemia. Hence, two different chemically synthesized cHH enantiomers, containing either L- or D-Phe(3), were injected to the circulation of intermolt females following removal of their X organ-Sinus gland complex. The effects triggered by the injection of the two alternate isomers were detected after one hour through measurement of circulating glucose levels. Triggered changes of the transcriptome expression profile in the hepatopancreas were analyzed by RNA-seq. A whole transcriptome shotgun sequence assembly provided the assumedly complete transcriptome of P. leptodactylus hepatopancreas, followed by RNA-seq analysis of changes in the expression level of many genes caused by the application of each of the hormone isomers. Circulating glucose levels were much higher in response to the D-isoform than to the L-isoform injection, one hour from injection. Similarly, the RNA-seq analysis confirmed a stronger effect on gene expression following the administration of D-cHH, while just limited alterations were caused by the L-isomer. These findings demonstrated a more prominent short term effect of the D-cHH on the transcription profile and shed light on the effect of the D-isomer on specific functional gene groups. Another contribution of the study is the construction of a de novo assembly of the hepatopancreas transcriptome, consisting of 39,935 contigs, that dramatically increases the molecular information available for this species and for crustaceans in general, providing an efficient tool for studying gene expression patterns in this organ.</description><subject>Animals</subject><subject>Arthropod Proteins - pharmacology</subject><subject>Assembly</subject><subject>Astacoidea - genetics</subject><subject>Bioinformatics</subject><subject>Biology</subject><subject>Crayfish</subject><subject>Crustaceans</subject><subject>Decapoda</subject><subject>Enantiomers</subject><subject>Female</subject><subject>Females</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Glucose</subject><subject>Glucose metabolism</subject><subject>Glycolysis</subject><subject>Glycolysis - drug effects</subject><subject>Glycolysis - genetics</subject><subject>Hepatopancreas</subject><subject>Hepatopancreas - drug effects</subject><subject>Hepatopancreas - enzymology</subject><subject>Hepatopancreas - metabolism</subject><subject>Hormones</subject><subject>Hyperglycemia</subject><subject>Injection</subject><subject>Invertebrate Hormones - pharmacology</subject><subject>Isomers</subject><subject>Marsupenaeus japonicus</subject><subject>Metabolic Networks and Pathways - drug effects</subject><subject>Metabolic Networks and Pathways - genetics</subject><subject>Metabolism</subject><subject>Nerve Tissue Proteins - pharmacology</subject><subject>Neuropeptides</subject><subject>Nucleotide sequence</subject><subject>Ontology</subject><subject>Orconectes limosus</subject><subject>Peptidases</subject><subject>Peptide Hydrolases - genetics</subject><subject>Peptides</subject><subject>Periclimenaeus leptodactylus</subject><subject>Physiology</subject><subject>Procambarus clarkii</subject><subject>Proteases</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Sinus</subject><subject>Sinus gland</subject><subject>Transcription</subject><subject>Transcription (Genetics)</subject><subject>Transcription, Genetic - 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Results of a Transcriptomic Study</title><author>Manfrin, Chiara ; Tom, Moshe ; De Moro, Gianluca ; Gerdol, Marco ; Guarnaccia, Corrado ; Mosco, Alessandro ; Pallavicini, Alberto ; Giulianini, Piero Giulio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-bc6cbdb0f22a9a4a31533700864b577258a21c2f098b1a2e81bb61ac45f9c84e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Arthropod Proteins - pharmacology</topic><topic>Assembly</topic><topic>Astacoidea - genetics</topic><topic>Bioinformatics</topic><topic>Biology</topic><topic>Crayfish</topic><topic>Crustaceans</topic><topic>Decapoda</topic><topic>Enantiomers</topic><topic>Female</topic><topic>Females</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Glucose</topic><topic>Glucose metabolism</topic><topic>Glycolysis</topic><topic>Glycolysis - 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Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manfrin, Chiara</au><au>Tom, Moshe</au><au>De Moro, Gianluca</au><au>Gerdol, Marco</au><au>Guarnaccia, Corrado</au><au>Mosco, Alessandro</au><au>Pallavicini, Alberto</au><au>Giulianini, Piero Giulio</au><au>Buratti, Emanuele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of D-Crustacean Hyperglycemic Hormone Induces Peptidases Transcription and Suppresses Glycolysis-Related Transcripts in the Hepatopancreas of the Crayfish Pontastacus leptodactylus - Results of a Transcriptomic Study</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-06-19</date><risdate>2013</risdate><volume>8</volume><issue>6</issue><spage>e65176</spage><pages>e65176-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The crustacean Hyperglycemic Hormone (cHH) is a neuropeptide present in many decapods. Two different chiral isomers are simultaneously present in Astacid crayfish and their specific biological functions are still poorly understood. The present study is aimed at better understanding the potentially different effect of each of the isomers on the hepatopancreatic gene expression profile in the crayfish Pontastacus leptodactylus, in the context of short term hyperglycemia. Hence, two different chemically synthesized cHH enantiomers, containing either L- or D-Phe(3), were injected to the circulation of intermolt females following removal of their X organ-Sinus gland complex. The effects triggered by the injection of the two alternate isomers were detected after one hour through measurement of circulating glucose levels. Triggered changes of the transcriptome expression profile in the hepatopancreas were analyzed by RNA-seq. A whole transcriptome shotgun sequence assembly provided the assumedly complete transcriptome of P. leptodactylus hepatopancreas, followed by RNA-seq analysis of changes in the expression level of many genes caused by the application of each of the hormone isomers. Circulating glucose levels were much higher in response to the D-isoform than to the L-isoform injection, one hour from injection. Similarly, the RNA-seq analysis confirmed a stronger effect on gene expression following the administration of D-cHH, while just limited alterations were caused by the L-isomer. These findings demonstrated a more prominent short term effect of the D-cHH on the transcription profile and shed light on the effect of the D-isomer on specific functional gene groups. Another contribution of the study is the construction of a de novo assembly of the hepatopancreas transcriptome, consisting of 39,935 contigs, that dramatically increases the molecular information available for this species and for crustaceans in general, providing an efficient tool for studying gene expression patterns in this organ.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23840318</pmid><doi>10.1371/journal.pone.0065176</doi><tpages>e65176</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-06, Vol.8 (6), p.e65176 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1371458520 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Arthropod Proteins - pharmacology Assembly Astacoidea - genetics Bioinformatics Biology Crayfish Crustaceans Decapoda Enantiomers Female Females Gene expression Gene Expression Profiling Gene Expression Regulation, Enzymologic - drug effects Genes Genetic engineering Genomes Genomics Glucose Glucose metabolism Glycolysis Glycolysis - drug effects Glycolysis - genetics Hepatopancreas Hepatopancreas - drug effects Hepatopancreas - enzymology Hepatopancreas - metabolism Hormones Hyperglycemia Injection Invertebrate Hormones - pharmacology Isomers Marsupenaeus japonicus Metabolic Networks and Pathways - drug effects Metabolic Networks and Pathways - genetics Metabolism Nerve Tissue Proteins - pharmacology Neuropeptides Nucleotide sequence Ontology Orconectes limosus Peptidases Peptide Hydrolases - genetics Peptides Periclimenaeus leptodactylus Physiology Procambarus clarkii Proteases Proteins Ribonucleic acid RNA Sinus Sinus gland Transcription Transcription (Genetics) Transcription, Genetic - drug effects Transcriptome - drug effects |
| title | Application of D-Crustacean Hyperglycemic Hormone Induces Peptidases Transcription and Suppresses Glycolysis-Related Transcripts in the Hepatopancreas of the Crayfish Pontastacus leptodactylus - Results of a Transcriptomic Study |
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