Characteristics and stimulation potential with BMP-2 and BMP-7 of tenocyte-like cells isolated from the rotator cuff of female donors
Tendon bone healing of the rotator cuff is often associated with non-healing or recurrent defects, which seems to be influenced by the patient's age and sex. The present study aims to examine cellular biological characteristics of tenocyte-like cells that may contribute to this impaired rotator...
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| Veröffentlicht in: | PloS one 2013-06, Vol.8 (6), p.e67209-e67209 |
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| creator | Klatte-Schulz, Franka Pauly, Stephan Scheibel, Markus Greiner, Stefan Gerhardt, Christian Hartwig, Jelka Schmidmaier, Gerhard Wildemann, Britt |
| description | Tendon bone healing of the rotator cuff is often associated with non-healing or recurrent defects, which seems to be influenced by the patient's age and sex. The present study aims to examine cellular biological characteristics of tenocyte-like cells that may contribute to this impaired rotator cuff healing. Moreover, a therapeutic approach using growth factors could possibly stimulate tendon bone healing. Therefore, our second aim was to identify patient groups who would particularly benefit from growth factor stimulation. Tenocyte-like cells isolated from supraspinatus tendons of female donors younger and older than 65 years of age were characterized with respect to different cellular biological parameters, such as cell density, cell count, marker expression, collagen-I protein synthesis, and stem cell potential. Furthermore, cells of the donor groups were stimulated with BMP-2 and BMP-7 (200 and 1000 ng/ml) in 3D-culture and analyzed for cell count, marker expression and collagen-I protein synthesis. Female donors older than 65 years of age showed significantly decreased cell count and collagen-I protein synthesis compared to cells from donors younger than 65 years. Cellular biological parameters including cell count, collagen-I and -III expression, and collagen-I protein synthesis of cells from both donor groups were stimulated with BMP-2 and BMP-7. The cells from donors older than 65 years revealed a decreased stimulation potential for cell count compared to the younger group. Cells from female donors older than 65 years of age showed inferior cellular biological characteristics. This may be one reason for a weaker healing potential observed in older female patients and should be taken into consideration for tendon bone healing of the rotator cuff. |
| doi_str_mv | 10.1371/journal.pone.0067209 |
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The present study aims to examine cellular biological characteristics of tenocyte-like cells that may contribute to this impaired rotator cuff healing. Moreover, a therapeutic approach using growth factors could possibly stimulate tendon bone healing. Therefore, our second aim was to identify patient groups who would particularly benefit from growth factor stimulation. Tenocyte-like cells isolated from supraspinatus tendons of female donors younger and older than 65 years of age were characterized with respect to different cellular biological parameters, such as cell density, cell count, marker expression, collagen-I protein synthesis, and stem cell potential. Furthermore, cells of the donor groups were stimulated with BMP-2 and BMP-7 (200 and 1000 ng/ml) in 3D-culture and analyzed for cell count, marker expression and collagen-I protein synthesis. Female donors older than 65 years of age showed significantly decreased cell count and collagen-I protein synthesis compared to cells from donors younger than 65 years. Cellular biological parameters including cell count, collagen-I and -III expression, and collagen-I protein synthesis of cells from both donor groups were stimulated with BMP-2 and BMP-7. The cells from donors older than 65 years revealed a decreased stimulation potential for cell count compared to the younger group. Cells from female donors older than 65 years of age showed inferior cellular biological characteristics. This may be one reason for a weaker healing potential observed in older female patients and should be taken into consideration for tendon bone healing of the rotator cuff.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0067209</identifier><identifier>PMID: 23825642</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age ; Aged ; Animals ; Biology ; Bone healing ; Bone marrow ; Bone morphogenetic protein 2 ; Bone Morphogenetic Protein 2 - pharmacology ; Bone morphogenetic protein 7 ; Bone Morphogenetic Protein 7 - pharmacology ; Cell culture ; Cell density ; Cell Differentiation - drug effects ; Cell Line ; Cell Separation ; Collagen ; Defects ; Female ; Gene expression ; Growth factors ; Healing ; Health aspects ; Humans ; Medical research ; Medicine ; Menstruation ; Mice ; Middle Aged ; Patients ; Protein biosynthesis ; Protein synthesis ; Proteins ; Rotator Cuff - cytology ; Shoulder ; Stem cells ; Stimulation ; Studies ; Surgery ; Tendons ; Tendons - cytology ; Tendons - drug effects ; Women</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e67209-e67209</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Klatte-Schulz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Klatte-Schulz et al 2013 Klatte-Schulz et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-43601fd9d636db473c3f25a4b2f6f914c05ca850865ad9d9c505915694849a453</citedby><cites>FETCH-LOGICAL-c692t-43601fd9d636db473c3f25a4b2f6f914c05ca850865ad9d9c505915694849a453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692432/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3692432/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23825642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klatte-Schulz, Franka</creatorcontrib><creatorcontrib>Pauly, Stephan</creatorcontrib><creatorcontrib>Scheibel, Markus</creatorcontrib><creatorcontrib>Greiner, Stefan</creatorcontrib><creatorcontrib>Gerhardt, Christian</creatorcontrib><creatorcontrib>Hartwig, Jelka</creatorcontrib><creatorcontrib>Schmidmaier, Gerhard</creatorcontrib><creatorcontrib>Wildemann, Britt</creatorcontrib><title>Characteristics and stimulation potential with BMP-2 and BMP-7 of tenocyte-like cells isolated from the rotator cuff of female donors</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Tendon bone healing of the rotator cuff is often associated with non-healing or recurrent defects, which seems to be influenced by the patient's age and sex. The present study aims to examine cellular biological characteristics of tenocyte-like cells that may contribute to this impaired rotator cuff healing. Moreover, a therapeutic approach using growth factors could possibly stimulate tendon bone healing. Therefore, our second aim was to identify patient groups who would particularly benefit from growth factor stimulation. Tenocyte-like cells isolated from supraspinatus tendons of female donors younger and older than 65 years of age were characterized with respect to different cellular biological parameters, such as cell density, cell count, marker expression, collagen-I protein synthesis, and stem cell potential. Furthermore, cells of the donor groups were stimulated with BMP-2 and BMP-7 (200 and 1000 ng/ml) in 3D-culture and analyzed for cell count, marker expression and collagen-I protein synthesis. Female donors older than 65 years of age showed significantly decreased cell count and collagen-I protein synthesis compared to cells from donors younger than 65 years. Cellular biological parameters including cell count, collagen-I and -III expression, and collagen-I protein synthesis of cells from both donor groups were stimulated with BMP-2 and BMP-7. The cells from donors older than 65 years revealed a decreased stimulation potential for cell count compared to the younger group. Cells from female donors older than 65 years of age showed inferior cellular biological characteristics. This may be one reason for a weaker healing potential observed in older female patients and should be taken into consideration for tendon bone healing of the rotator cuff.</description><subject>Age</subject><subject>Aged</subject><subject>Animals</subject><subject>Biology</subject><subject>Bone healing</subject><subject>Bone marrow</subject><subject>Bone morphogenetic protein 2</subject><subject>Bone Morphogenetic Protein 2 - pharmacology</subject><subject>Bone morphogenetic protein 7</subject><subject>Bone Morphogenetic Protein 7 - pharmacology</subject><subject>Cell culture</subject><subject>Cell density</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Cell Separation</subject><subject>Collagen</subject><subject>Defects</subject><subject>Female</subject><subject>Gene expression</subject><subject>Growth factors</subject><subject>Healing</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Menstruation</subject><subject>Mice</subject><subject>Middle Aged</subject><subject>Patients</subject><subject>Protein biosynthesis</subject><subject>Protein synthesis</subject><subject>Proteins</subject><subject>Rotator Cuff - cytology</subject><subject>Shoulder</subject><subject>Stem cells</subject><subject>Stimulation</subject><subject>Studies</subject><subject>Surgery</subject><subject>Tendons</subject><subject>Tendons - cytology</subject><subject>Tendons - drug effects</subject><subject>Women</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk8tu1DAUhiMEoqXwBggsISFYzOBbnHhTqYy4jFRUxG1rOY49cUniwXaAPgDvjTOTVhPUBcoiR_b3_875c5xljxFcIlKgV5du8L1sl1vX6yWErMCQ38mOESd4wTAkdw_qo-xBCJcQ5qRk7H52hEmJc0bxcfZn1UgvVdTehmhVALKvQaq6oZXRuh5sXdR9tLIFv2xswOsPHxd4B41VAZwBad-pq6gXrf2ugdJtG4ANLul1DYx3HYiNBt5FGZ0HajBmVBndyVaD2vXOh4fZPSPboB9N75Ps69s3X1bvF-cX79ars_OFYhzHBSUMIlPzmhFWV7QgihicS1phwwxHVMFcyTKHJctlorjKYc5RzjgtKZc0JyfZ073vtnVBTAkGMeZJUyaQJmK9J2onL8XW2076K-GkFbsF5zdC-hRUqwXUBSoqhCqsDOV1WVWGq0phWCtEy5Ikr9PptKHqdK1Sjl62M9P5Tm8bsXE_BUndUoKTwYvJwLsfgw5RdDaMActeu2H8bl5SQlJjCX32D3p7dxO1SdkL2xuXzlWjqTijRVlizGGRqOUtVHpq3VmVxs3YtD4TvJwJEhP177iRQwhi_fnT_7MX3-bs8wO20bKNTRqsYRzMMAfpHlTeheC1uQkZwV3_12mI8baI6bYk2ZPDH3Qjur4e5C8Rpg86</recordid><startdate>20130625</startdate><enddate>20130625</enddate><creator>Klatte-Schulz, Franka</creator><creator>Pauly, Stephan</creator><creator>Scheibel, Markus</creator><creator>Greiner, Stefan</creator><creator>Gerhardt, Christian</creator><creator>Hartwig, Jelka</creator><creator>Schmidmaier, Gerhard</creator><creator>Wildemann, Britt</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130625</creationdate><title>Characteristics and stimulation potential with BMP-2 and BMP-7 of tenocyte-like cells isolated from the rotator cuff of female donors</title><author>Klatte-Schulz, Franka ; Pauly, Stephan ; Scheibel, Markus ; Greiner, Stefan ; Gerhardt, Christian ; Hartwig, Jelka ; Schmidmaier, Gerhard ; Wildemann, Britt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-43601fd9d636db473c3f25a4b2f6f914c05ca850865ad9d9c505915694849a453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Age</topic><topic>Aged</topic><topic>Animals</topic><topic>Biology</topic><topic>Bone healing</topic><topic>Bone marrow</topic><topic>Bone morphogenetic protein 2</topic><topic>Bone Morphogenetic Protein 2 - 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The present study aims to examine cellular biological characteristics of tenocyte-like cells that may contribute to this impaired rotator cuff healing. Moreover, a therapeutic approach using growth factors could possibly stimulate tendon bone healing. Therefore, our second aim was to identify patient groups who would particularly benefit from growth factor stimulation. Tenocyte-like cells isolated from supraspinatus tendons of female donors younger and older than 65 years of age were characterized with respect to different cellular biological parameters, such as cell density, cell count, marker expression, collagen-I protein synthesis, and stem cell potential. Furthermore, cells of the donor groups were stimulated with BMP-2 and BMP-7 (200 and 1000 ng/ml) in 3D-culture and analyzed for cell count, marker expression and collagen-I protein synthesis. Female donors older than 65 years of age showed significantly decreased cell count and collagen-I protein synthesis compared to cells from donors younger than 65 years. Cellular biological parameters including cell count, collagen-I and -III expression, and collagen-I protein synthesis of cells from both donor groups were stimulated with BMP-2 and BMP-7. The cells from donors older than 65 years revealed a decreased stimulation potential for cell count compared to the younger group. Cells from female donors older than 65 years of age showed inferior cellular biological characteristics. This may be one reason for a weaker healing potential observed in older female patients and should be taken into consideration for tendon bone healing of the rotator cuff.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23825642</pmid><doi>10.1371/journal.pone.0067209</doi><tpages>e67209</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-06, Vol.8 (6), p.e67209-e67209 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1371423804 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Age Aged Animals Biology Bone healing Bone marrow Bone morphogenetic protein 2 Bone Morphogenetic Protein 2 - pharmacology Bone morphogenetic protein 7 Bone Morphogenetic Protein 7 - pharmacology Cell culture Cell density Cell Differentiation - drug effects Cell Line Cell Separation Collagen Defects Female Gene expression Growth factors Healing Health aspects Humans Medical research Medicine Menstruation Mice Middle Aged Patients Protein biosynthesis Protein synthesis Proteins Rotator Cuff - cytology Shoulder Stem cells Stimulation Studies Surgery Tendons Tendons - cytology Tendons - drug effects Women |
| title | Characteristics and stimulation potential with BMP-2 and BMP-7 of tenocyte-like cells isolated from the rotator cuff of female donors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T23%3A57%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characteristics%20and%20stimulation%20potential%20with%20BMP-2%20and%20BMP-7%20of%20tenocyte-like%20cells%20isolated%20from%20the%20rotator%20cuff%20of%20female%20donors&rft.jtitle=PloS%20one&rft.au=Klatte-Schulz,%20Franka&rft.date=2013-06-25&rft.volume=8&rft.issue=6&rft.spage=e67209&rft.epage=e67209&rft.pages=e67209-e67209&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0067209&rft_dat=%3Cgale_plos_%3EA478822907%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1371423804&rft_id=info:pmid/23825642&rft_galeid=A478822907&rft_doaj_id=oai_doaj_org_article_0e717b11b2cf49d8bbf9cbc20dc14883&rfr_iscdi=true |