'Cand. Actinochlamydia clariae' gen. nov., sp. nov., a unique intracellular bacterium causing epitheliocystis in catfish (Clarias gariepinus) in Uganda
Epitheliocystis, caused by bacteria infecting gill epithelial cells in fish, is common among a large range of fish species in both fresh- and seawater. The aquaculture industry considers epitheliocystis an important problem. It affects the welfare of the fish and the resulting gill disease may lead...
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| creator | Steigen, Andreas Nylund, Are Karlsbakk, Egil Akoll, Peter Fiksdal, Ingrid U Nylund, Stian Odong, Robinson Plarre, Heidrun Semyalo, Ronald Skår, Cecilie Watanabe, Kuninori |
| description | Epitheliocystis, caused by bacteria infecting gill epithelial cells in fish, is common among a large range of fish species in both fresh- and seawater. The aquaculture industry considers epitheliocystis an important problem. It affects the welfare of the fish and the resulting gill disease may lead to mortalities. In a culture facility in Kampala, Uganda, juveniles of the African sharptooth catfish (Clarias gariepinus) was observed swimming in the surface, sometimes belly up, showing signs of respiratory problems. Histological examination of gill tissues from this fish revealed large amounts of epitheliocysts, and also presence of a few Ichthyobodo sp. and Trichodina sp.
Sequencing of the epitheliocystis bacterium 16S rRNA gene shows 86.3% similarity with Candidatus Piscichlamydia salmonis causing epitheliocystis in Atlantic salmon (Salmo salar). Transmission electron microscopy showed that the morphology of the developmental stages of the bacterium is similar to that of members of the family Chlamydiaceae. The similarity of the bacterium rRNA gene sequences compared with other chlamydia-like bacteria ranged between 80.5% and 86.3%. Inclusions containing this new bacterium have tubules/channels (termed actinae) that are radiating from the inclusion membrane and opening on the cell surface or in neighbouring cells.
Radiation of tubules/channels (actinae) from the inclusion membrane has never been described in any of the other members of Chlamydiales. It seems to be a completely new character and an apomorphy. We propose the name Candidatus Actinochlamydia clariae gen. nov., sp. nov. (Actinochlamydiaceae fam. nov., order Chlamydiales, phylum Chlamydiae) for this new agent causing epitheliocystis in African sharptooth catfish. |
| doi_str_mv | 10.1371/journal.pone.0066840 |
| format | Article |
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Sequencing of the epitheliocystis bacterium 16S rRNA gene shows 86.3% similarity with Candidatus Piscichlamydia salmonis causing epitheliocystis in Atlantic salmon (Salmo salar). Transmission electron microscopy showed that the morphology of the developmental stages of the bacterium is similar to that of members of the family Chlamydiaceae. The similarity of the bacterium rRNA gene sequences compared with other chlamydia-like bacteria ranged between 80.5% and 86.3%. Inclusions containing this new bacterium have tubules/channels (termed actinae) that are radiating from the inclusion membrane and opening on the cell surface or in neighbouring cells.
Radiation of tubules/channels (actinae) from the inclusion membrane has never been described in any of the other members of Chlamydiales. It seems to be a completely new character and an apomorphy. We propose the name Candidatus Actinochlamydia clariae gen. nov., sp. nov. (Actinochlamydiaceae fam. nov., order Chlamydiales, phylum Chlamydiae) for this new agent causing epitheliocystis in African sharptooth catfish.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0066840</identifier><identifier>PMID: 23826156</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Animal tissues ; Animals ; Aquaculture ; Bacteria ; Bacterial infections ; Bacterial Infections - microbiology ; Biology ; Candidatus Piscichlamydia salmonis ; Catfish ; Catfishes - microbiology ; Cell culture ; Cell surface ; Channels ; Chemical analysis ; Chlamydia ; Chlamydia psittaci ; Chlamydia trachomatis ; Chlamydiae ; Chlamydiales - physiology ; Chlamydiales - ultrastructure ; Clarias gariepinus ; Cysts ; Deoxyribonucleic acid ; Developmental stages ; DNA ; Electron microscopy ; Epithelial cells ; Epithelium - microbiology ; Epithelium - pathology ; Epithelium - ultrastructure ; Fish ; Fish Diseases - microbiology ; Fish Diseases - pathology ; Gene sequencing ; Gills - microbiology ; Gills - pathology ; Inclusion Bodies - pathology ; Inclusion Bodies - ultrastructure ; Intracellular Space - microbiology ; Medicine ; Microscopy ; Molecular Sequence Data ; Morphology ; New species ; Phylogeny ; Proteins ; Radiation ; Real-Time Polymerase Chain Reaction ; Respiratory function ; rRNA 16S ; Salmo salar ; Salmon ; Seawater ; Sexually transmitted diseases ; Similarity ; STD ; Swimming ; Transmission electron microscopy ; Tubules ; Uganda ; Veterinary Science ; Water analysis ; Zoology</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e66840</ispartof><rights>2013 Steigen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Steigen et al 2013 Steigen et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-8255416f30465baca8c11c05ec8e6ab8928f0e46dfa560fc1abb25e47e5fa8fb3</citedby><cites>FETCH-LOGICAL-c526t-8255416f30465baca8c11c05ec8e6ab8928f0e46dfa560fc1abb25e47e5fa8fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691252/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3691252/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23826156$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Horn, Matthias</contributor><creatorcontrib>Steigen, Andreas</creatorcontrib><creatorcontrib>Nylund, Are</creatorcontrib><creatorcontrib>Karlsbakk, Egil</creatorcontrib><creatorcontrib>Akoll, Peter</creatorcontrib><creatorcontrib>Fiksdal, Ingrid U</creatorcontrib><creatorcontrib>Nylund, Stian</creatorcontrib><creatorcontrib>Odong, Robinson</creatorcontrib><creatorcontrib>Plarre, Heidrun</creatorcontrib><creatorcontrib>Semyalo, Ronald</creatorcontrib><creatorcontrib>Skår, Cecilie</creatorcontrib><creatorcontrib>Watanabe, Kuninori</creatorcontrib><title>'Cand. Actinochlamydia clariae' gen. nov., sp. nov., a unique intracellular bacterium causing epitheliocystis in catfish (Clarias gariepinus) in Uganda</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Epitheliocystis, caused by bacteria infecting gill epithelial cells in fish, is common among a large range of fish species in both fresh- and seawater. The aquaculture industry considers epitheliocystis an important problem. It affects the welfare of the fish and the resulting gill disease may lead to mortalities. In a culture facility in Kampala, Uganda, juveniles of the African sharptooth catfish (Clarias gariepinus) was observed swimming in the surface, sometimes belly up, showing signs of respiratory problems. Histological examination of gill tissues from this fish revealed large amounts of epitheliocysts, and also presence of a few Ichthyobodo sp. and Trichodina sp.
Sequencing of the epitheliocystis bacterium 16S rRNA gene shows 86.3% similarity with Candidatus Piscichlamydia salmonis causing epitheliocystis in Atlantic salmon (Salmo salar). Transmission electron microscopy showed that the morphology of the developmental stages of the bacterium is similar to that of members of the family Chlamydiaceae. The similarity of the bacterium rRNA gene sequences compared with other chlamydia-like bacteria ranged between 80.5% and 86.3%. Inclusions containing this new bacterium have tubules/channels (termed actinae) that are radiating from the inclusion membrane and opening on the cell surface or in neighbouring cells.
Radiation of tubules/channels (actinae) from the inclusion membrane has never been described in any of the other members of Chlamydiales. It seems to be a completely new character and an apomorphy. We propose the name Candidatus Actinochlamydia clariae gen. nov., sp. nov. (Actinochlamydiaceae fam. nov., order Chlamydiales, phylum Chlamydiae) for this new agent causing epitheliocystis in African sharptooth catfish.</description><subject>Agriculture</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Aquaculture</subject><subject>Bacteria</subject><subject>Bacterial infections</subject><subject>Bacterial Infections - microbiology</subject><subject>Biology</subject><subject>Candidatus Piscichlamydia salmonis</subject><subject>Catfish</subject><subject>Catfishes - microbiology</subject><subject>Cell culture</subject><subject>Cell surface</subject><subject>Channels</subject><subject>Chemical analysis</subject><subject>Chlamydia</subject><subject>Chlamydia psittaci</subject><subject>Chlamydia trachomatis</subject><subject>Chlamydiae</subject><subject>Chlamydiales - physiology</subject><subject>Chlamydiales - ultrastructure</subject><subject>Clarias gariepinus</subject><subject>Cysts</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental stages</subject><subject>DNA</subject><subject>Electron microscopy</subject><subject>Epithelial cells</subject><subject>Epithelium - microbiology</subject><subject>Epithelium - pathology</subject><subject>Epithelium - ultrastructure</subject><subject>Fish</subject><subject>Fish Diseases - microbiology</subject><subject>Fish Diseases - pathology</subject><subject>Gene sequencing</subject><subject>Gills - microbiology</subject><subject>Gills - pathology</subject><subject>Inclusion Bodies - pathology</subject><subject>Inclusion Bodies - ultrastructure</subject><subject>Intracellular Space - microbiology</subject><subject>Medicine</subject><subject>Microscopy</subject><subject>Molecular Sequence Data</subject><subject>Morphology</subject><subject>New species</subject><subject>Phylogeny</subject><subject>Proteins</subject><subject>Radiation</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Respiratory function</subject><subject>rRNA 16S</subject><subject>Salmo salar</subject><subject>Salmon</subject><subject>Seawater</subject><subject>Sexually transmitted diseases</subject><subject>Similarity</subject><subject>STD</subject><subject>Swimming</subject><subject>Transmission electron microscopy</subject><subject>Tubules</subject><subject>Uganda</subject><subject>Veterinary Science</subject><subject>Water analysis</subject><subject>Zoology</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNp1kt1qGzEQhZfS0qRp36C0gl6khXqrf2tvCsH0JxDoTXMtZrXSWmYtudJuwE_S160cr0Ny0SsNmm_OGQ5TVW8Jrglbki-bOKUAQ72LwdYYS6k4fladk4bRhaSYPX9Un1Wvct5gLJiS8mV1Rpmikgh5Xv29XEHoanRlRh-iWQ-w3XcekBkgebCXqLehRiHe1Z9R3p0qQFPwfyaLfBgTGDsMU-FRC2a0yU9bZGDKPvTI7vy4toOPZp9HnwtfWqPzeY0-ru4tMurLU7gw5U-H_m1fFoLX1QsHQ7Zv5veiuv3-7ffq5-Lm14_r1dXNwggqx4WiQnAiHcNcimIPyhBisLBGWQmtaqhy2HLZORASO0OgbamwfGmFA-VadlG9P-ruhpj1nGnWJWHcYLLkqhDXR6KLsNG75LeQ9jqC1_cfMfUa0ujNYLXDTSdbaRwzDQdBWs6kUcpQKvmycU3R-jq7Te3WdsYe4hueiD7tBL_WfbzTTDaECloEPswCKZb88_iflfmRMinmnKx7cCD4wJHTlD4cj56Pp4y9e7zdw9DpWtg_J6zFBw</recordid><startdate>20130624</startdate><enddate>20130624</enddate><creator>Steigen, Andreas</creator><creator>Nylund, Are</creator><creator>Karlsbakk, Egil</creator><creator>Akoll, Peter</creator><creator>Fiksdal, Ingrid U</creator><creator>Nylund, Stian</creator><creator>Odong, Robinson</creator><creator>Plarre, Heidrun</creator><creator>Semyalo, Ronald</creator><creator>Skår, Cecilie</creator><creator>Watanabe, Kuninori</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130624</creationdate><title>'Cand. 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Peter</au><au>Fiksdal, Ingrid U</au><au>Nylund, Stian</au><au>Odong, Robinson</au><au>Plarre, Heidrun</au><au>Semyalo, Ronald</au><au>Skår, Cecilie</au><au>Watanabe, Kuninori</au><au>Horn, Matthias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>'Cand. Actinochlamydia clariae' gen. nov., sp. nov., a unique intracellular bacterium causing epitheliocystis in catfish (Clarias gariepinus) in Uganda</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-06-24</date><risdate>2013</risdate><volume>8</volume><issue>6</issue><spage>e66840</spage><pages>e66840-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Epitheliocystis, caused by bacteria infecting gill epithelial cells in fish, is common among a large range of fish species in both fresh- and seawater. The aquaculture industry considers epitheliocystis an important problem. It affects the welfare of the fish and the resulting gill disease may lead to mortalities. In a culture facility in Kampala, Uganda, juveniles of the African sharptooth catfish (Clarias gariepinus) was observed swimming in the surface, sometimes belly up, showing signs of respiratory problems. Histological examination of gill tissues from this fish revealed large amounts of epitheliocysts, and also presence of a few Ichthyobodo sp. and Trichodina sp.
Sequencing of the epitheliocystis bacterium 16S rRNA gene shows 86.3% similarity with Candidatus Piscichlamydia salmonis causing epitheliocystis in Atlantic salmon (Salmo salar). Transmission electron microscopy showed that the morphology of the developmental stages of the bacterium is similar to that of members of the family Chlamydiaceae. The similarity of the bacterium rRNA gene sequences compared with other chlamydia-like bacteria ranged between 80.5% and 86.3%. Inclusions containing this new bacterium have tubules/channels (termed actinae) that are radiating from the inclusion membrane and opening on the cell surface or in neighbouring cells.
Radiation of tubules/channels (actinae) from the inclusion membrane has never been described in any of the other members of Chlamydiales. It seems to be a completely new character and an apomorphy. We propose the name Candidatus Actinochlamydia clariae gen. nov., sp. nov. (Actinochlamydiaceae fam. nov., order Chlamydiales, phylum Chlamydiae) for this new agent causing epitheliocystis in African sharptooth catfish.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23826156</pmid><doi>10.1371/journal.pone.0066840</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-06, Vol.8 (6), p.e66840 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1370901748 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agriculture Animal tissues Animals Aquaculture Bacteria Bacterial infections Bacterial Infections - microbiology Biology Candidatus Piscichlamydia salmonis Catfish Catfishes - microbiology Cell culture Cell surface Channels Chemical analysis Chlamydia Chlamydia psittaci Chlamydia trachomatis Chlamydiae Chlamydiales - physiology Chlamydiales - ultrastructure Clarias gariepinus Cysts Deoxyribonucleic acid Developmental stages DNA Electron microscopy Epithelial cells Epithelium - microbiology Epithelium - pathology Epithelium - ultrastructure Fish Fish Diseases - microbiology Fish Diseases - pathology Gene sequencing Gills - microbiology Gills - pathology Inclusion Bodies - pathology Inclusion Bodies - ultrastructure Intracellular Space - microbiology Medicine Microscopy Molecular Sequence Data Morphology New species Phylogeny Proteins Radiation Real-Time Polymerase Chain Reaction Respiratory function rRNA 16S Salmo salar Salmon Seawater Sexually transmitted diseases Similarity STD Swimming Transmission electron microscopy Tubules Uganda Veterinary Science Water analysis Zoology |
| title | 'Cand. Actinochlamydia clariae' gen. nov., sp. nov., a unique intracellular bacterium causing epitheliocystis in catfish (Clarias gariepinus) in Uganda |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T13%3A18%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle='Cand.%20Actinochlamydia%20clariae'%20gen.%20nov.,%20sp.%20nov.,%20a%20unique%20intracellular%20bacterium%20causing%20epitheliocystis%20in%20catfish%20(Clarias%20gariepinus)%20in%20Uganda&rft.jtitle=PloS%20one&rft.au=Steigen,%20Andreas&rft.date=2013-06-24&rft.volume=8&rft.issue=6&rft.spage=e66840&rft.pages=e66840-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0066840&rft_dat=%3Cproquest_plos_%3E3003897771%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1370901748&rft_id=info:pmid/23826156&rft_doaj_id=oai_doaj_org_article_f09d6b6cf3c94a51b436c88c226479f9&rfr_iscdi=true |