Curcumin attenuates acute graft-versus-host disease severity via in vivo regulations on Th1, Th17 and regulatory T cells
In this study we examined the in vivo and in vitro effects and mechanisms of action of curcumin on the development of acute graft-versus-host disease (GVHD) using a murine model. Mixed lymphocyte reactions were used to determine the in vitro effects of curcumin. Treatment with curcumin attenuated al...
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| Veröffentlicht in: | PloS one 2013-06, Vol.8 (6), p.e67171-e67171 |
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| creator | Park, Min-Jung Moon, Su-Jin Lee, Sung-Hee Yang, Eun-Ji Min, Jun-Ki Cho, Seok-Goo Yang, Chul-Woo Park, Sung-Hwan Kim, Ho-Youn Cho, Mi-La |
| description | In this study we examined the in vivo and in vitro effects and mechanisms of action of curcumin on the development of acute graft-versus-host disease (GVHD) using a murine model.
Mixed lymphocyte reactions were used to determine the in vitro effects of curcumin. Treatment with curcumin attenuated alloreactive T cell proliferation and inhibited the production of interferon (IFN)-γ and interleukin (IL)-17. In a murine acute GVHD model, transplantation of curcumin-treated allogeneic splenocytes into irradiated recipient mice significantly reduced the clinical severity scores of acute GVHD manifested in the liver, skin, colon and lung as compared with animals receiving vehicle-treated splenocytes. c-Fos and c-Jun expression levels in the skin and intestine, which are major target organs, were analyzed using immunohistochemical staining. Expression of both proteins was reduced in epithelial tissues of skin and intestine from curcumin-treated GVHD animals. The IFN-γ-expressing CD4(+) splenocytes and IFN-γ-expressing lymph node cells were dramatically decreased in curcumin-treated mice. In contrast, CD4(+)Foxp3(+) splenocytes were increased in the curcumin-treated acute GVHD animals. Flow cytometric analysis revealed that animals transplanted with curcumin-treated allogeneic splenocytes showed increased populations of CD4(+) regulatory T cells (Tregs) as well as CD8(+) Treg cells, compared to animals administered vehicle-treated splenocytes. Curcumin-treated acute GVHD animals could have a change in B cell subpopulations.
In the present study, we investigated the efficacy and mechanism of action of curcumin treatment against acute GVHD. The acute GVHD mice administered with curcumin-treated splenocytes showed significantly reduced severity of acute GVHD. Curcumin exerted in vivo preventive effects on acute GVHD by reciprocal regulation of T helper 1 (Th1) and Treg (both CD4(+) and CD8(+) Treg) cell lineages as well as B cell homeostasis. |
| doi_str_mv | 10.1371/journal.pone.0067171 |
| format | Article |
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Mixed lymphocyte reactions were used to determine the in vitro effects of curcumin. Treatment with curcumin attenuated alloreactive T cell proliferation and inhibited the production of interferon (IFN)-γ and interleukin (IL)-17. In a murine acute GVHD model, transplantation of curcumin-treated allogeneic splenocytes into irradiated recipient mice significantly reduced the clinical severity scores of acute GVHD manifested in the liver, skin, colon and lung as compared with animals receiving vehicle-treated splenocytes. c-Fos and c-Jun expression levels in the skin and intestine, which are major target organs, were analyzed using immunohistochemical staining. Expression of both proteins was reduced in epithelial tissues of skin and intestine from curcumin-treated GVHD animals. The IFN-γ-expressing CD4(+) splenocytes and IFN-γ-expressing lymph node cells were dramatically decreased in curcumin-treated mice. In contrast, CD4(+)Foxp3(+) splenocytes were increased in the curcumin-treated acute GVHD animals. Flow cytometric analysis revealed that animals transplanted with curcumin-treated allogeneic splenocytes showed increased populations of CD4(+) regulatory T cells (Tregs) as well as CD8(+) Treg cells, compared to animals administered vehicle-treated splenocytes. Curcumin-treated acute GVHD animals could have a change in B cell subpopulations.
In the present study, we investigated the efficacy and mechanism of action of curcumin treatment against acute GVHD. The acute GVHD mice administered with curcumin-treated splenocytes showed significantly reduced severity of acute GVHD. Curcumin exerted in vivo preventive effects on acute GVHD by reciprocal regulation of T helper 1 (Th1) and Treg (both CD4(+) and CD8(+) Treg) cell lineages as well as B cell homeostasis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0067171</identifier><identifier>PMID: 23840617</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal models ; Animal tissues ; Animals ; Antigens ; Apoptosis ; B-Lymphocytes - drug effects ; B-Lymphocytes - immunology ; Biological response modifiers ; Biology ; Bone marrow ; Bone Marrow Transplantation ; c-Fos protein ; c-Jun protein ; CD4 antigen ; CD8 antigen ; Cell Proliferation ; Cells, Cultured ; Colon ; Colon - drug effects ; Colon - metabolism ; Colon - pathology ; Comparative analysis ; Curcumin ; Curcumin - pharmacology ; Curcumin - therapeutic use ; Disease ; Flow cytometry ; Fos protein ; Foxp3 protein ; Gene expression ; Graft vs Host Disease - drug therapy ; Graft vs Host Disease - immunology ; Graft-versus-host reaction ; Helper cells ; Homeostasis ; Immunohistochemistry ; Immunologic Factors - pharmacology ; Immunologic Factors - therapeutic use ; Immunology ; Immunoregulation ; In vivo methods and tests ; Interferon ; Interferon-gamma - metabolism ; Interleukin-17 - metabolism ; Interleukins ; Internal medicine ; Intestine ; Liver ; Lungs ; Lymph nodes ; Lymphocyte Culture Test, Mixed ; Lymphocytes ; Lymphocytes B ; Lymphocytes T ; Lymphoma ; Medicine ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Organs ; Proteins ; Rheumatism ; Rheumatology ; Science ; Skin ; Skin - drug effects ; Skin - metabolism ; Skin - pathology ; Spleen - immunology ; Spleen - pathology ; Splenocytes ; Stem cells ; Subpopulations ; T cells ; T-Lymphocytes, Regulatory - drug effects ; T-Lymphocytes, Regulatory - immunology ; Th1 Cells - drug effects ; Th1 Cells - immunology ; Th17 Cells - drug effects ; Th17 Cells - immunology ; Transcription Factor AP-1 - antagonists & inhibitors ; Transcription Factor AP-1 - metabolism ; Transcription factors ; Transplantation ; Transplants & implants ; Tumor necrosis factor-TNF ; γ-Interferon</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e67171-e67171</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Park et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Park et al 2013 Park et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-fc1383fea81fe0c323b5b00bd05a0e6cb3886b81991e484ea6aa8275c368b99b3</citedby><cites>FETCH-LOGICAL-c692t-fc1383fea81fe0c323b5b00bd05a0e6cb3886b81991e484ea6aa8275c368b99b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688629/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688629/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23840617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Min-Jung</creatorcontrib><creatorcontrib>Moon, Su-Jin</creatorcontrib><creatorcontrib>Lee, Sung-Hee</creatorcontrib><creatorcontrib>Yang, Eun-Ji</creatorcontrib><creatorcontrib>Min, Jun-Ki</creatorcontrib><creatorcontrib>Cho, Seok-Goo</creatorcontrib><creatorcontrib>Yang, Chul-Woo</creatorcontrib><creatorcontrib>Park, Sung-Hwan</creatorcontrib><creatorcontrib>Kim, Ho-Youn</creatorcontrib><creatorcontrib>Cho, Mi-La</creatorcontrib><title>Curcumin attenuates acute graft-versus-host disease severity via in vivo regulations on Th1, Th17 and regulatory T cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In this study we examined the in vivo and in vitro effects and mechanisms of action of curcumin on the development of acute graft-versus-host disease (GVHD) using a murine model.
Mixed lymphocyte reactions were used to determine the in vitro effects of curcumin. Treatment with curcumin attenuated alloreactive T cell proliferation and inhibited the production of interferon (IFN)-γ and interleukin (IL)-17. In a murine acute GVHD model, transplantation of curcumin-treated allogeneic splenocytes into irradiated recipient mice significantly reduced the clinical severity scores of acute GVHD manifested in the liver, skin, colon and lung as compared with animals receiving vehicle-treated splenocytes. c-Fos and c-Jun expression levels in the skin and intestine, which are major target organs, were analyzed using immunohistochemical staining. Expression of both proteins was reduced in epithelial tissues of skin and intestine from curcumin-treated GVHD animals. The IFN-γ-expressing CD4(+) splenocytes and IFN-γ-expressing lymph node cells were dramatically decreased in curcumin-treated mice. In contrast, CD4(+)Foxp3(+) splenocytes were increased in the curcumin-treated acute GVHD animals. Flow cytometric analysis revealed that animals transplanted with curcumin-treated allogeneic splenocytes showed increased populations of CD4(+) regulatory T cells (Tregs) as well as CD8(+) Treg cells, compared to animals administered vehicle-treated splenocytes. Curcumin-treated acute GVHD animals could have a change in B cell subpopulations.
In the present study, we investigated the efficacy and mechanism of action of curcumin treatment against acute GVHD. The acute GVHD mice administered with curcumin-treated splenocytes showed significantly reduced severity of acute GVHD. Curcumin exerted in vivo preventive effects on acute GVHD by reciprocal regulation of T helper 1 (Th1) and Treg (both CD4(+) and CD8(+) Treg) cell lineages as well as B cell homeostasis.</description><subject>Animal models</subject><subject>Animal tissues</subject><subject>Animals</subject><subject>Antigens</subject><subject>Apoptosis</subject><subject>B-Lymphocytes - drug effects</subject><subject>B-Lymphocytes - immunology</subject><subject>Biological response modifiers</subject><subject>Biology</subject><subject>Bone marrow</subject><subject>Bone Marrow Transplantation</subject><subject>c-Fos protein</subject><subject>c-Jun protein</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Colon</subject><subject>Colon - drug effects</subject><subject>Colon - metabolism</subject><subject>Colon - pathology</subject><subject>Comparative analysis</subject><subject>Curcumin</subject><subject>Curcumin - pharmacology</subject><subject>Curcumin - therapeutic use</subject><subject>Disease</subject><subject>Flow cytometry</subject><subject>Fos protein</subject><subject>Foxp3 protein</subject><subject>Gene expression</subject><subject>Graft vs Host Disease - drug therapy</subject><subject>Graft vs Host Disease - immunology</subject><subject>Graft-versus-host reaction</subject><subject>Helper cells</subject><subject>Homeostasis</subject><subject>Immunohistochemistry</subject><subject>Immunologic Factors - pharmacology</subject><subject>Immunologic Factors - therapeutic use</subject><subject>Immunology</subject><subject>Immunoregulation</subject><subject>In vivo methods and tests</subject><subject>Interferon</subject><subject>Interferon-gamma - metabolism</subject><subject>Interleukin-17 - metabolism</subject><subject>Interleukins</subject><subject>Internal medicine</subject><subject>Intestine</subject><subject>Liver</subject><subject>Lungs</subject><subject>Lymph nodes</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Lymphocytes</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Lymphoma</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Organs</subject><subject>Proteins</subject><subject>Rheumatism</subject><subject>Rheumatology</subject><subject>Science</subject><subject>Skin</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><subject>Skin - pathology</subject><subject>Spleen - immunology</subject><subject>Spleen - pathology</subject><subject>Splenocytes</subject><subject>Stem cells</subject><subject>Subpopulations</subject><subject>T cells</subject><subject>T-Lymphocytes, Regulatory - drug effects</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>Th1 Cells - drug effects</subject><subject>Th1 Cells - immunology</subject><subject>Th17 Cells - drug effects</subject><subject>Th17 Cells - immunology</subject><subject>Transcription Factor AP-1 - antagonists & inhibitors</subject><subject>Transcription Factor AP-1 - metabolism</subject><subject>Transcription factors</subject><subject>Transplantation</subject><subject>Transplants & implants</subject><subject>Tumor necrosis factor-TNF</subject><subject>γ-Interferon</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk29r2zAQxs3YWLtu32BsgsHYYM4ky7HlN4MS9idQKGzZ3oqzfHZUHCuV5NB8-8mNU-LRF8Ngi9PvHt091kXRa0ZnjOfs843pbQftbGs6nFGa5SxnT6JzVvAkzhLKn56sz6IXzt1QOuciy55HZwkXKc1Yfh7dLXqr-o3uCHiPXQ8eHQHVeySNhdrHO7Sud_HaOE8q7RAcEochqv2e7DSQkLrTO0MsNn0LXpvOEdOR1Zp9Gl45ga46bhq7JyuisG3dy-hZDa3DV-P3Ivr97etq8SO-uv6-XFxexSorEh_XinHBawTBaqSKJ7ycl5SWFZ0DxUyVXIisFKwoGKYiRcgARJLPFc9EWRQlv4jeHnS3rXFyNM3JYCFlItiRB2J5ICoDN3Jr9QbsXhrQ8j5gbCPBeq1alFjRpMAi5cHrFEVaKkjKQqg6qThwSIPWl_G0vtxgpbDzFtqJ6HSn02vZmJ0M5YosKYLAh1HAmtsenZcb7QbDoEPTD3UXxZymeT7U_e4f9PHuRqqB0IDuahPOVYOovEzzYFzC83mgZo9Q4alwo1W4YbUO8UnCx0lCYDze-QZ65-Ty18__Z6__TNn3J-waofVrZ9r-_l5NwfQAKmucs1g_mMzo0D87uiGHAZHjgIS0N6c_6CHpOBH8L355C_0</recordid><startdate>20130620</startdate><enddate>20130620</enddate><creator>Park, Min-Jung</creator><creator>Moon, Su-Jin</creator><creator>Lee, Sung-Hee</creator><creator>Yang, Eun-Ji</creator><creator>Min, Jun-Ki</creator><creator>Cho, Seok-Goo</creator><creator>Yang, Chul-Woo</creator><creator>Park, Sung-Hwan</creator><creator>Kim, Ho-Youn</creator><creator>Cho, Mi-La</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130620</creationdate><title>Curcumin attenuates acute graft-versus-host disease severity via in vivo regulations on Th1, Th17 and regulatory T cells</title><author>Park, Min-Jung ; Moon, Su-Jin ; Lee, Sung-Hee ; Yang, Eun-Ji ; Min, Jun-Ki ; Cho, Seok-Goo ; Yang, Chul-Woo ; Park, Sung-Hwan ; Kim, Ho-Youn ; Cho, Mi-La</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-fc1383fea81fe0c323b5b00bd05a0e6cb3886b81991e484ea6aa8275c368b99b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animal models</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Antigens</topic><topic>Apoptosis</topic><topic>B-Lymphocytes - drug effects</topic><topic>B-Lymphocytes - immunology</topic><topic>Biological response modifiers</topic><topic>Biology</topic><topic>Bone marrow</topic><topic>Bone Marrow Transplantation</topic><topic>c-Fos protein</topic><topic>c-Jun protein</topic><topic>CD4 antigen</topic><topic>CD8 antigen</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Colon</topic><topic>Colon - drug effects</topic><topic>Colon - metabolism</topic><topic>Colon - pathology</topic><topic>Comparative analysis</topic><topic>Curcumin</topic><topic>Curcumin - pharmacology</topic><topic>Curcumin - therapeutic use</topic><topic>Disease</topic><topic>Flow cytometry</topic><topic>Fos protein</topic><topic>Foxp3 protein</topic><topic>Gene expression</topic><topic>Graft vs Host Disease - drug therapy</topic><topic>Graft vs Host Disease - immunology</topic><topic>Graft-versus-host reaction</topic><topic>Helper cells</topic><topic>Homeostasis</topic><topic>Immunohistochemistry</topic><topic>Immunologic Factors - pharmacology</topic><topic>Immunologic Factors - therapeutic use</topic><topic>Immunology</topic><topic>Immunoregulation</topic><topic>In vivo methods and tests</topic><topic>Interferon</topic><topic>Interferon-gamma - metabolism</topic><topic>Interleukin-17 - metabolism</topic><topic>Interleukins</topic><topic>Internal medicine</topic><topic>Intestine</topic><topic>Liver</topic><topic>Lungs</topic><topic>Lymph nodes</topic><topic>Lymphocyte Culture Test, Mixed</topic><topic>Lymphocytes</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Lymphoma</topic><topic>Medicine</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Organs</topic><topic>Proteins</topic><topic>Rheumatism</topic><topic>Rheumatology</topic><topic>Science</topic><topic>Skin</topic><topic>Skin - drug effects</topic><topic>Skin - metabolism</topic><topic>Skin - pathology</topic><topic>Spleen - immunology</topic><topic>Spleen - pathology</topic><topic>Splenocytes</topic><topic>Stem cells</topic><topic>Subpopulations</topic><topic>T cells</topic><topic>T-Lymphocytes, Regulatory - drug effects</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>Th1 Cells - drug effects</topic><topic>Th1 Cells - immunology</topic><topic>Th17 Cells - drug effects</topic><topic>Th17 Cells - immunology</topic><topic>Transcription Factor AP-1 - antagonists & inhibitors</topic><topic>Transcription Factor AP-1 - metabolism</topic><topic>Transcription factors</topic><topic>Transplantation</topic><topic>Transplants & implants</topic><topic>Tumor necrosis factor-TNF</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Min-Jung</creatorcontrib><creatorcontrib>Moon, Su-Jin</creatorcontrib><creatorcontrib>Lee, Sung-Hee</creatorcontrib><creatorcontrib>Yang, Eun-Ji</creatorcontrib><creatorcontrib>Min, Jun-Ki</creatorcontrib><creatorcontrib>Cho, Seok-Goo</creatorcontrib><creatorcontrib>Yang, Chul-Woo</creatorcontrib><creatorcontrib>Park, Sung-Hwan</creatorcontrib><creatorcontrib>Kim, Ho-Youn</creatorcontrib><creatorcontrib>Cho, Mi-La</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Min-Jung</au><au>Moon, Su-Jin</au><au>Lee, Sung-Hee</au><au>Yang, Eun-Ji</au><au>Min, Jun-Ki</au><au>Cho, Seok-Goo</au><au>Yang, Chul-Woo</au><au>Park, Sung-Hwan</au><au>Kim, Ho-Youn</au><au>Cho, Mi-La</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Curcumin attenuates acute graft-versus-host disease severity via in vivo regulations on Th1, Th17 and regulatory T cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-06-20</date><risdate>2013</risdate><volume>8</volume><issue>6</issue><spage>e67171</spage><epage>e67171</epage><pages>e67171-e67171</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In this study we examined the in vivo and in vitro effects and mechanisms of action of curcumin on the development of acute graft-versus-host disease (GVHD) using a murine model.
Mixed lymphocyte reactions were used to determine the in vitro effects of curcumin. Treatment with curcumin attenuated alloreactive T cell proliferation and inhibited the production of interferon (IFN)-γ and interleukin (IL)-17. In a murine acute GVHD model, transplantation of curcumin-treated allogeneic splenocytes into irradiated recipient mice significantly reduced the clinical severity scores of acute GVHD manifested in the liver, skin, colon and lung as compared with animals receiving vehicle-treated splenocytes. c-Fos and c-Jun expression levels in the skin and intestine, which are major target organs, were analyzed using immunohistochemical staining. Expression of both proteins was reduced in epithelial tissues of skin and intestine from curcumin-treated GVHD animals. The IFN-γ-expressing CD4(+) splenocytes and IFN-γ-expressing lymph node cells were dramatically decreased in curcumin-treated mice. In contrast, CD4(+)Foxp3(+) splenocytes were increased in the curcumin-treated acute GVHD animals. Flow cytometric analysis revealed that animals transplanted with curcumin-treated allogeneic splenocytes showed increased populations of CD4(+) regulatory T cells (Tregs) as well as CD8(+) Treg cells, compared to animals administered vehicle-treated splenocytes. Curcumin-treated acute GVHD animals could have a change in B cell subpopulations.
In the present study, we investigated the efficacy and mechanism of action of curcumin treatment against acute GVHD. The acute GVHD mice administered with curcumin-treated splenocytes showed significantly reduced severity of acute GVHD. Curcumin exerted in vivo preventive effects on acute GVHD by reciprocal regulation of T helper 1 (Th1) and Treg (both CD4(+) and CD8(+) Treg) cell lineages as well as B cell homeostasis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23840617</pmid><doi>10.1371/journal.pone.0067171</doi><tpages>e67171</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-06, Vol.8 (6), p.e67171-e67171 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1370183867 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animal models Animal tissues Animals Antigens Apoptosis B-Lymphocytes - drug effects B-Lymphocytes - immunology Biological response modifiers Biology Bone marrow Bone Marrow Transplantation c-Fos protein c-Jun protein CD4 antigen CD8 antigen Cell Proliferation Cells, Cultured Colon Colon - drug effects Colon - metabolism Colon - pathology Comparative analysis Curcumin Curcumin - pharmacology Curcumin - therapeutic use Disease Flow cytometry Fos protein Foxp3 protein Gene expression Graft vs Host Disease - drug therapy Graft vs Host Disease - immunology Graft-versus-host reaction Helper cells Homeostasis Immunohistochemistry Immunologic Factors - pharmacology Immunologic Factors - therapeutic use Immunology Immunoregulation In vivo methods and tests Interferon Interferon-gamma - metabolism Interleukin-17 - metabolism Interleukins Internal medicine Intestine Liver Lungs Lymph nodes Lymphocyte Culture Test, Mixed Lymphocytes Lymphocytes B Lymphocytes T Lymphoma Medicine Mice Mice, Inbred BALB C Mice, Inbred C57BL Organs Proteins Rheumatism Rheumatology Science Skin Skin - drug effects Skin - metabolism Skin - pathology Spleen - immunology Spleen - pathology Splenocytes Stem cells Subpopulations T cells T-Lymphocytes, Regulatory - drug effects T-Lymphocytes, Regulatory - immunology Th1 Cells - drug effects Th1 Cells - immunology Th17 Cells - drug effects Th17 Cells - immunology Transcription Factor AP-1 - antagonists & inhibitors Transcription Factor AP-1 - metabolism Transcription factors Transplantation Transplants & implants Tumor necrosis factor-TNF γ-Interferon |
| title | Curcumin attenuates acute graft-versus-host disease severity via in vivo regulations on Th1, Th17 and regulatory T cells |
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