microRNAs Involved in Regulating Spontaneous Recovery in Embolic Stroke Model
To date, miRNA expression studies on cerebral ischemia in both human and animal models have focused mainly on acute phase of ischemic stroke. In this study, we present the roles played by microRNAs in the spontaneous recovery phases in cerebral ischemia using rodent stroke models. Brain tissues were...
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| description | To date, miRNA expression studies on cerebral ischemia in both human and animal models have focused mainly on acute phase of ischemic stroke. In this study, we present the roles played by microRNAs in the spontaneous recovery phases in cerebral ischemia using rodent stroke models. Brain tissues were harvested at different reperfusion time points ranging from 0-168 hrs after middle cerebral artery occlusion using homologous emboli. MiRNA and mRNA expression profiles were investigated by microarray followed by multiple statistical analysis. Candidate transcripts were also validated by quantitative RT-PCR. Three specific groups of miRNAs were observed among a total of 346 differentially expressed miRNAs. miRNAs, miR-21, -142-3p, -142-5p, and -146a displayed significant upregulation during stroke recovery (48 hrs to 168 hrs) compared with those during acute phases (0 hrs to 24 hrs). On the other hand, an opposite trend was observed in the expression of miR-196a/b/c, -224 and -324-3p. Interestingly, miR-206, -290, -291a-5p and -30c-1*, positively correlated with the infarct sizes, with an initial increase up to 24hrs followed by a gradual decrease from 48 hrs to 168 hrs (R = 0.95). Taken together with the expression levels of corresponding mRNA targets, we have also found that Hedgehog, Notch, Wnt and TGF-β signaling pathways could play significant roles in stroke recovery and especially in neuronal repair. |
| doi_str_mv | 10.1371/journal.pone.0066393 |
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In this study, we present the roles played by microRNAs in the spontaneous recovery phases in cerebral ischemia using rodent stroke models. Brain tissues were harvested at different reperfusion time points ranging from 0-168 hrs after middle cerebral artery occlusion using homologous emboli. MiRNA and mRNA expression profiles were investigated by microarray followed by multiple statistical analysis. Candidate transcripts were also validated by quantitative RT-PCR. Three specific groups of miRNAs were observed among a total of 346 differentially expressed miRNAs. miRNAs, miR-21, -142-3p, -142-5p, and -146a displayed significant upregulation during stroke recovery (48 hrs to 168 hrs) compared with those during acute phases (0 hrs to 24 hrs). On the other hand, an opposite trend was observed in the expression of miR-196a/b/c, -224 and -324-3p. Interestingly, miR-206, -290, -291a-5p and -30c-1*, positively correlated with the infarct sizes, with an initial increase up to 24hrs followed by a gradual decrease from 48 hrs to 168 hrs (R = 0.95). Taken together with the expression levels of corresponding mRNA targets, we have also found that Hedgehog, Notch, Wnt and TGF-β signaling pathways could play significant roles in stroke recovery and especially in neuronal repair.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0066393</identifier><identifier>PMID: 23823624</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal models ; Animals ; Apoptosis ; Biochemistry ; Biology ; Brain ; Cell adhesion & migration ; Cells, Cultured ; Cerebral blood flow ; Communication ; Disease Models, Animal ; Disease Progression ; DNA microarrays ; Embolism - complications ; Gene expression ; Homology ; Ischemia ; Laboratory animals ; Male ; Mathematics ; Medical prognosis ; Medicine ; Mice ; MicroRNA ; MicroRNAs ; MicroRNAs - physiology ; miRNA ; Occlusion ; Polymerase chain reaction ; Rats ; Rats, Wistar ; Real-Time Polymerase Chain Reaction ; Recovery ; Reperfusion ; Rodents ; Signaling ; Spontaneous recovery ; Statistical analysis ; Stroke ; Stroke - etiology ; Stroke - physiopathology ; Tissues ; Transcriptome ; Wnt protein</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e66393-e66393</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Liu et al 2013 Liu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-9063f4c942ea23f3355f56a015e3da457ce4e82c2fae21cb7181c433b3ce31903</citedby><cites>FETCH-LOGICAL-c758t-9063f4c942ea23f3355f56a015e3da457ce4e82c2fae21cb7181c433b3ce31903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688919/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688919/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23823624$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Fu Jia</creatorcontrib><creatorcontrib>Lim, Kai Ying</creatorcontrib><creatorcontrib>Kaur, Prameet</creatorcontrib><creatorcontrib>Sepramaniam, Sugunavathi</creatorcontrib><creatorcontrib>Armugam, Arunmozhiarasi</creatorcontrib><creatorcontrib>Wong, Peter Tsun Hon</creatorcontrib><creatorcontrib>Jeyaseelan, Kandiah</creatorcontrib><title>microRNAs Involved in Regulating Spontaneous Recovery in Embolic Stroke Model</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>To date, miRNA expression studies on cerebral ischemia in both human and animal models have focused mainly on acute phase of ischemic stroke. In this study, we present the roles played by microRNAs in the spontaneous recovery phases in cerebral ischemia using rodent stroke models. Brain tissues were harvested at different reperfusion time points ranging from 0-168 hrs after middle cerebral artery occlusion using homologous emboli. MiRNA and mRNA expression profiles were investigated by microarray followed by multiple statistical analysis. Candidate transcripts were also validated by quantitative RT-PCR. Three specific groups of miRNAs were observed among a total of 346 differentially expressed miRNAs. miRNAs, miR-21, -142-3p, -142-5p, and -146a displayed significant upregulation during stroke recovery (48 hrs to 168 hrs) compared with those during acute phases (0 hrs to 24 hrs). On the other hand, an opposite trend was observed in the expression of miR-196a/b/c, -224 and -324-3p. Interestingly, miR-206, -290, -291a-5p and -30c-1*, positively correlated with the infarct sizes, with an initial increase up to 24hrs followed by a gradual decrease from 48 hrs to 168 hrs (R = 0.95). Taken together with the expression levels of corresponding mRNA targets, we have also found that Hedgehog, Notch, Wnt and TGF-β signaling pathways could play significant roles in stroke recovery and especially in neuronal repair.</description><subject>Animal models</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Biology</subject><subject>Brain</subject><subject>Cell adhesion & migration</subject><subject>Cells, Cultured</subject><subject>Cerebral blood flow</subject><subject>Communication</subject><subject>Disease Models, Animal</subject><subject>Disease Progression</subject><subject>DNA microarrays</subject><subject>Embolism - complications</subject><subject>Gene expression</subject><subject>Homology</subject><subject>Ischemia</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Mathematics</subject><subject>Medical prognosis</subject><subject>Medicine</subject><subject>Mice</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - physiology</subject><subject>miRNA</subject><subject>Occlusion</subject><subject>Polymerase chain reaction</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Recovery</subject><subject>Reperfusion</subject><subject>Rodents</subject><subject>Signaling</subject><subject>Spontaneous recovery</subject><subject>Statistical analysis</subject><subject>Stroke</subject><subject>Stroke - etiology</subject><subject>Stroke - physiopathology</subject><subject>Tissues</subject><subject>Transcriptome</subject><subject>Wnt protein</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNklFv0zAQxyMEYqPwDRBEQkLw0GL7Eid-QaqmAZU2JrXAq-U6l9TFjUucVOzb49BsatAekB9snX_3P93dP4peUjKjkNEPW9c1tbKzvatxRgjnIOBRdE4FsClnBB6fvM-iZ95vCUkh5_xpdMYgZ8BZch5d74xu3PLr3MeL-uDsAYvY1PESq86q1tRVvAoFWlWj63wIa3fA5rZHLndrZ42OV23jfmJ87Qq0z6MnpbIeXwz3JPr-6fLbxZfp1c3nxcX8aqqzNG-ngnAoEy0ShopBCZCmZcoVoSlCoZI005hgzjQrFTKq1xnNqU4A1qARqCAwiV4fdffWeTlMwksKXAARkOaBWByJwqmt3Ddmp5pb6ZSRfwOuqaRqWqMtyiwha52hVkKIRBAUquR5XnJMiYKcQND6OFTr1jssNNZto-xIdPxTm42s3EFC0BFhCZPo3SDQuF8d-lbujNdo7XGskmaCZgmkvO_szT_ow90NVKVCA6YuXaire1E5T7KcsbQHJ9HsASqcAsPWg21KE-KjhPejhMC0-LutVOe9XKyW_8_e_Bizb0_YDSrbbryzXWtc7cdgcgSDJ71vsLwfMiWyd_3dNGTvejm4PqS9Ol3QfdKdzeEPsXD7Ug</recordid><startdate>20130618</startdate><enddate>20130618</enddate><creator>Liu, Fu Jia</creator><creator>Lim, Kai Ying</creator><creator>Kaur, Prameet</creator><creator>Sepramaniam, Sugunavathi</creator><creator>Armugam, Arunmozhiarasi</creator><creator>Wong, Peter Tsun Hon</creator><creator>Jeyaseelan, Kandiah</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130618</creationdate><title>microRNAs Involved in Regulating Spontaneous Recovery in Embolic Stroke Model</title><author>Liu, Fu Jia ; Lim, Kai Ying ; Kaur, Prameet ; Sepramaniam, Sugunavathi ; Armugam, Arunmozhiarasi ; Wong, Peter Tsun Hon ; Jeyaseelan, Kandiah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-9063f4c942ea23f3355f56a015e3da457ce4e82c2fae21cb7181c433b3ce31903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Biochemistry</topic><topic>Biology</topic><topic>Brain</topic><topic>Cell adhesion & migration</topic><topic>Cells, Cultured</topic><topic>Cerebral blood flow</topic><topic>Communication</topic><topic>Disease Models, Animal</topic><topic>Disease Progression</topic><topic>DNA microarrays</topic><topic>Embolism - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Fu Jia</au><au>Lim, Kai Ying</au><au>Kaur, Prameet</au><au>Sepramaniam, Sugunavathi</au><au>Armugam, Arunmozhiarasi</au><au>Wong, Peter Tsun Hon</au><au>Jeyaseelan, Kandiah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>microRNAs Involved in Regulating Spontaneous Recovery in Embolic Stroke Model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-06-18</date><risdate>2013</risdate><volume>8</volume><issue>6</issue><spage>e66393</spage><epage>e66393</epage><pages>e66393-e66393</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>To date, miRNA expression studies on cerebral ischemia in both human and animal models have focused mainly on acute phase of ischemic stroke. In this study, we present the roles played by microRNAs in the spontaneous recovery phases in cerebral ischemia using rodent stroke models. Brain tissues were harvested at different reperfusion time points ranging from 0-168 hrs after middle cerebral artery occlusion using homologous emboli. MiRNA and mRNA expression profiles were investigated by microarray followed by multiple statistical analysis. Candidate transcripts were also validated by quantitative RT-PCR. Three specific groups of miRNAs were observed among a total of 346 differentially expressed miRNAs. miRNAs, miR-21, -142-3p, -142-5p, and -146a displayed significant upregulation during stroke recovery (48 hrs to 168 hrs) compared with those during acute phases (0 hrs to 24 hrs). On the other hand, an opposite trend was observed in the expression of miR-196a/b/c, -224 and -324-3p. Interestingly, miR-206, -290, -291a-5p and -30c-1*, positively correlated with the infarct sizes, with an initial increase up to 24hrs followed by a gradual decrease from 48 hrs to 168 hrs (R = 0.95). Taken together with the expression levels of corresponding mRNA targets, we have also found that Hedgehog, Notch, Wnt and TGF-β signaling pathways could play significant roles in stroke recovery and especially in neuronal repair.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23823624</pmid><doi>10.1371/journal.pone.0066393</doi><tpages>e66393</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animal models Animals Apoptosis Biochemistry Biology Brain Cell adhesion & migration Cells, Cultured Cerebral blood flow Communication Disease Models, Animal Disease Progression DNA microarrays Embolism - complications Gene expression Homology Ischemia Laboratory animals Male Mathematics Medical prognosis Medicine Mice MicroRNA MicroRNAs MicroRNAs - physiology miRNA Occlusion Polymerase chain reaction Rats Rats, Wistar Real-Time Polymerase Chain Reaction Recovery Reperfusion Rodents Signaling Spontaneous recovery Statistical analysis Stroke Stroke - etiology Stroke - physiopathology Tissues Transcriptome Wnt protein |
| title | microRNAs Involved in Regulating Spontaneous Recovery in Embolic Stroke Model |
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