Characterization of hair follicle development in engineered skin substitutes
Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human kera...
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| Veröffentlicht in: | PloS one 2013-06, Vol.8 (6), p.e65664-e65664 |
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| creator | Sriwiriyanont, Penkanok Lynch, Kaari A McFarland, Kevin L Supp, Dorothy M Boyce, Steven T |
| description | Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human keratinocytes from neonatal foreskins and cultured murine dermal papilla cells from adult GFP transgenic mice and grafted orthotopically to full-thickness wounds on athymic mice. Non-cultured dissociated neonatal murine-only skin cells, or cultured human-only skin keratinocytes and fibroblasts without dermal papilla cells served as positive and negative controls respectively. In this study, neonatal murine-only skin substitutes formed external hairs and sebaceous glands, chimeric skin substitutes formed pigmented hairs without sebaceous glands, and human-only skin substitutes formed no follicles or glands. Although chimeric hair cannot erupt readily, removal of upper skin layer exposed keratinized hair shafts at the skin surface. Development of incomplete pilosebaceous units in chimeric hair corresponded with upregulation of hair-related genes, LEF1 and WNT10B, and downregulation of a marker of sebaceous glands, Steroyl-CoA desaturase. Transepidermal water loss was normal in all conditions. This study demonstrated that while sebaceous glands may be involved in hair eruption, they are not required for hair development in engineered skin substitutes. |
| doi_str_mv | 10.1371/journal.pone.0065664 |
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To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human keratinocytes from neonatal foreskins and cultured murine dermal papilla cells from adult GFP transgenic mice and grafted orthotopically to full-thickness wounds on athymic mice. Non-cultured dissociated neonatal murine-only skin cells, or cultured human-only skin keratinocytes and fibroblasts without dermal papilla cells served as positive and negative controls respectively. In this study, neonatal murine-only skin substitutes formed external hairs and sebaceous glands, chimeric skin substitutes formed pigmented hairs without sebaceous glands, and human-only skin substitutes formed no follicles or glands. Although chimeric hair cannot erupt readily, removal of upper skin layer exposed keratinized hair shafts at the skin surface. Development of incomplete pilosebaceous units in chimeric hair corresponded with upregulation of hair-related genes, LEF1 and WNT10B, and downregulation of a marker of sebaceous glands, Steroyl-CoA desaturase. Transepidermal water loss was normal in all conditions. This study demonstrated that while sebaceous glands may be involved in hair eruption, they are not required for hair development in engineered skin substitutes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0065664</identifier><identifier>PMID: 23799033</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Appendages ; Biology ; Cells, Cultured ; Collagen ; Dermatology ; Desaturase ; Excavation ; Feasibility studies ; Fibroblasts ; Follicles ; Genetic engineering ; Glands ; Growth factors ; Hair ; Hair Follicle - growth & development ; Keratinocytes ; Medicine ; Mice ; Mice, Nude ; Neonates ; Real-Time Polymerase Chain Reaction ; Regeneration ; Sebaceous glands ; Sebaceous Glands - physiology ; Skin ; Skin, Artificial ; Stem cells ; Studies ; Substitutes ; Surgery ; Transgenic mice ; Water loss ; Wounds</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e65664-e65664</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Sriwiriyanont et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Sriwiriyanont et al 2013 Sriwiriyanont et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-71041609c76c67db7bd8e731efd56b826f54ef73a8894c137679b1752604d7173</citedby><cites>FETCH-LOGICAL-c692t-71041609c76c67db7bd8e731efd56b826f54ef73a8894c137679b1752604d7173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684595/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3684595/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23799033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sriwiriyanont, Penkanok</creatorcontrib><creatorcontrib>Lynch, Kaari A</creatorcontrib><creatorcontrib>McFarland, Kevin L</creatorcontrib><creatorcontrib>Supp, Dorothy M</creatorcontrib><creatorcontrib>Boyce, Steven T</creatorcontrib><title>Characterization of hair follicle development in engineered skin substitutes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human keratinocytes from neonatal foreskins and cultured murine dermal papilla cells from adult GFP transgenic mice and grafted orthotopically to full-thickness wounds on athymic mice. Non-cultured dissociated neonatal murine-only skin cells, or cultured human-only skin keratinocytes and fibroblasts without dermal papilla cells served as positive and negative controls respectively. In this study, neonatal murine-only skin substitutes formed external hairs and sebaceous glands, chimeric skin substitutes formed pigmented hairs without sebaceous glands, and human-only skin substitutes formed no follicles or glands. Although chimeric hair cannot erupt readily, removal of upper skin layer exposed keratinized hair shafts at the skin surface. Development of incomplete pilosebaceous units in chimeric hair corresponded with upregulation of hair-related genes, LEF1 and WNT10B, and downregulation of a marker of sebaceous glands, Steroyl-CoA desaturase. Transepidermal water loss was normal in all conditions. This study demonstrated that while sebaceous glands may be involved in hair eruption, they are not required for hair development in engineered skin substitutes.</description><subject>Animals</subject><subject>Appendages</subject><subject>Biology</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>Dermatology</subject><subject>Desaturase</subject><subject>Excavation</subject><subject>Feasibility studies</subject><subject>Fibroblasts</subject><subject>Follicles</subject><subject>Genetic engineering</subject><subject>Glands</subject><subject>Growth factors</subject><subject>Hair</subject><subject>Hair Follicle - growth & development</subject><subject>Keratinocytes</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Neonates</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Regeneration</subject><subject>Sebaceous glands</subject><subject>Sebaceous Glands - physiology</subject><subject>Skin</subject><subject>Skin, Artificial</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Substitutes</subject><subject>Surgery</subject><subject>Transgenic mice</subject><subject>Water loss</subject><subject>Wounds</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkkuP0zAUhSMEYoaBf4AgEhKCRYsdJ35skEYVj0qVRuK1tRznunVx42I7I-DX49DOqEGzQFkksb97rs_xLYqnGM0xYfjN1g-hV26-9z3MEaINpfW94hwLUs1ohcj9k--z4lGMW4Qawil9WJxVhAmBCDkvVouNCkonCPa3Stb3pTflRtlQGu-c1Q7KDq7B-f0O-lTavoR-bXuAAF0Zv-f_OLQx2TQkiI-LB0a5CE-O74vi6_t3XxYfZ6urD8vF5WqmqajSjGFUY4qEZlRT1rWs7TgwgsF0DW15RU1Tg2FEcS5qnc1SJlrMmoqiumOYkYvi-UF373yUxyCixIRyyppakEwsD0Tn1Vbug92p8Et6ZeXfBR_WUoU02pNAq5owk1NirOaGC0y4Rly3iGjOtMlab4_dhnYHnc45BOUmotOd3m7k2l_LfJy6EU0WeHUUCP7HADHJnY0anFM9-GE8N6uoaCganb34B73b3ZFaq2zA9sbnvnoUlZc14xURDRrbzu-g8tPBzuo8Ncbm9UnB60lBZhL8TGs1xCiXnz_9P3v1bcq-PGE3oFzaRO-GcdziFKwPoA4-xgDmNmSMxpTwTRpyHHp5HPpc9uz0gm6Lbqac_AFBAfsN</recordid><startdate>20130617</startdate><enddate>20130617</enddate><creator>Sriwiriyanont, Penkanok</creator><creator>Lynch, Kaari A</creator><creator>McFarland, Kevin L</creator><creator>Supp, Dorothy M</creator><creator>Boyce, Steven T</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130617</creationdate><title>Characterization of hair follicle development in engineered skin substitutes</title><author>Sriwiriyanont, Penkanok ; Lynch, Kaari A ; McFarland, Kevin L ; Supp, Dorothy M ; Boyce, Steven T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-71041609c76c67db7bd8e731efd56b826f54ef73a8894c137679b1752604d7173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Appendages</topic><topic>Biology</topic><topic>Cells, Cultured</topic><topic>Collagen</topic><topic>Dermatology</topic><topic>Desaturase</topic><topic>Excavation</topic><topic>Feasibility studies</topic><topic>Fibroblasts</topic><topic>Follicles</topic><topic>Genetic engineering</topic><topic>Glands</topic><topic>Growth factors</topic><topic>Hair</topic><topic>Hair Follicle - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sriwiriyanont, Penkanok</au><au>Lynch, Kaari A</au><au>McFarland, Kevin L</au><au>Supp, Dorothy M</au><au>Boyce, Steven T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of hair follicle development in engineered skin substitutes</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-06-17</date><risdate>2013</risdate><volume>8</volume><issue>6</issue><spage>e65664</spage><epage>e65664</epage><pages>e65664-e65664</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Generation of skin appendages in engineered skin substitutes has been limited by lack of trichogenic potency in cultured postnatal cells. To investigate the feasibility and the limitation of hair regeneration, engineered skin substitutes were prepared with chimeric populations of cultured human keratinocytes from neonatal foreskins and cultured murine dermal papilla cells from adult GFP transgenic mice and grafted orthotopically to full-thickness wounds on athymic mice. Non-cultured dissociated neonatal murine-only skin cells, or cultured human-only skin keratinocytes and fibroblasts without dermal papilla cells served as positive and negative controls respectively. In this study, neonatal murine-only skin substitutes formed external hairs and sebaceous glands, chimeric skin substitutes formed pigmented hairs without sebaceous glands, and human-only skin substitutes formed no follicles or glands. Although chimeric hair cannot erupt readily, removal of upper skin layer exposed keratinized hair shafts at the skin surface. Development of incomplete pilosebaceous units in chimeric hair corresponded with upregulation of hair-related genes, LEF1 and WNT10B, and downregulation of a marker of sebaceous glands, Steroyl-CoA desaturase. Transepidermal water loss was normal in all conditions. This study demonstrated that while sebaceous glands may be involved in hair eruption, they are not required for hair development in engineered skin substitutes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23799033</pmid><doi>10.1371/journal.pone.0065664</doi><tpages>e65664</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Appendages Biology Cells, Cultured Collagen Dermatology Desaturase Excavation Feasibility studies Fibroblasts Follicles Genetic engineering Glands Growth factors Hair Hair Follicle - growth & development Keratinocytes Medicine Mice Mice, Nude Neonates Real-Time Polymerase Chain Reaction Regeneration Sebaceous glands Sebaceous Glands - physiology Skin Skin, Artificial Stem cells Studies Substitutes Surgery Transgenic mice Water loss Wounds |
| title | Characterization of hair follicle development in engineered skin substitutes |
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