A wheat WRKY transcription factor TaWRKY10 confers tolerance to multiple abiotic stresses in transgenic tobacco
WRKY transcription factors are reported to be involved in defense regulation, stress response and plant growth and development. However, the precise role of WRKY transcription factors in abiotic stress tolerance is not completely understood, especially in crops. In this study, we identified and clon...
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| Veröffentlicht in: | PloS one 2013-06, Vol.8 (6), p.e65120 |
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| description | WRKY transcription factors are reported to be involved in defense regulation, stress response and plant growth and development. However, the precise role of WRKY transcription factors in abiotic stress tolerance is not completely understood, especially in crops. In this study, we identified and cloned 10 WRKY genes from genome of wheat (Triticum aestivum L.). TaWRKY10, a gene induced by multiple stresses, was selected for further investigation. TaWRKY10 was upregulated by treatment with polyethylene glycol, NaCl, cold and H2O2. Result of Southern blot indicates that the wheat genome contains three copies of TaWRKY10. The TaWRKY10 protein is localized in the nucleus and functions as a transcriptional activator. Overexpression of TaWRKY10 in tobacco (Nicotiana tabacum L.) resulted in enhanced drought and salt stress tolerance, mainly demonstrated by the transgenic plants exhibiting of increased germination rate, root length, survival rate, and relative water content under these stress conditions. Further investigation showed that transgenic plants also retained higher proline and soluble sugar contents, and lower reactive oxygen species and malonaldehyde contents. Moreover, overexpression of the TaWRKY10 regulated the expression of a series of stress related genes. Taken together, our results indicate that TaWRKY10 functions as a positive factor under drought and salt stresses by regulating the osmotic balance, ROS scavenging and transcription of stress related genes. |
| doi_str_mv | 10.1371/journal.pone.0065120 |
| format | Article |
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However, the precise role of WRKY transcription factors in abiotic stress tolerance is not completely understood, especially in crops. In this study, we identified and cloned 10 WRKY genes from genome of wheat (Triticum aestivum L.). TaWRKY10, a gene induced by multiple stresses, was selected for further investigation. TaWRKY10 was upregulated by treatment with polyethylene glycol, NaCl, cold and H2O2. Result of Southern blot indicates that the wheat genome contains three copies of TaWRKY10. The TaWRKY10 protein is localized in the nucleus and functions as a transcriptional activator. Overexpression of TaWRKY10 in tobacco (Nicotiana tabacum L.) resulted in enhanced drought and salt stress tolerance, mainly demonstrated by the transgenic plants exhibiting of increased germination rate, root length, survival rate, and relative water content under these stress conditions. Further investigation showed that transgenic plants also retained higher proline and soluble sugar contents, and lower reactive oxygen species and malonaldehyde contents. Moreover, overexpression of the TaWRKY10 regulated the expression of a series of stress related genes. Taken together, our results indicate that TaWRKY10 functions as a positive factor under drought and salt stresses by regulating the osmotic balance, ROS scavenging and transcription of stress related genes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0065120</identifier><identifier>PMID: 23762295</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abiotic factors ; Abiotic stress ; Adaptation, Physiological ; Agriculture ; Amino acids ; Animals ; Arabidopsis ; Barley ; Biology ; Biophysics ; Blotting, Southern ; Blotting, Western ; Cloning, Molecular ; Cold ; Cold Temperature ; Cold treatment ; DNA binding proteins ; Drought ; Droughts ; Education ; Gene expression ; Genes ; Genetic engineering ; Genetically modified plants ; Genomes ; Genomics ; Germination ; Germination - physiology ; Glycine max ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Immunoenzyme Techniques ; Immunoglobulin G - immunology ; International cooperation ; Kinases ; Laboratories ; Life sciences ; Malonaldehyde ; Malondialdehyde ; Moisture content ; Nicotiana - genetics ; Nicotiana - growth & development ; Nicotiana - metabolism ; Nuclei ; Oxidants - pharmacology ; Oxygen ; Physiology ; Plant growth ; Plant Proteins - genetics ; Plant Proteins - immunology ; Plant Proteins - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Polyethylene ; Polyethylene glycol ; Proline ; Proteins ; Rabbits ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Salinity tolerance ; Salt Tolerance ; Salts ; Signal transduction ; Sodium chloride ; Sodium Chloride - pharmacology ; Stress ; Stress, Physiological ; Stresses ; Subcellular Fractions ; Sugar ; Survival ; Tobacco ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcriptional Activation ; Transgenic plants ; Trends ; Triticum - genetics ; Triticum - metabolism ; Triticum aestivum ; Water content ; Wheat</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e65120</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Wang et al 2013 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6070-ba68828861080b45869ae5744fb176b2689422d0365254480298faadad3c29803</citedby><cites>FETCH-LOGICAL-c6070-ba68828861080b45869ae5744fb176b2689422d0365254480298faadad3c29803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677898/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3677898/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23762295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Deng, Pengyi</creatorcontrib><creatorcontrib>Chen, Liulin</creatorcontrib><creatorcontrib>Wang, Xiatian</creatorcontrib><creatorcontrib>Ma, Hui</creatorcontrib><creatorcontrib>Hu, Wei</creatorcontrib><creatorcontrib>Yao, Ningcong</creatorcontrib><creatorcontrib>Feng, Ying</creatorcontrib><creatorcontrib>Chai, Ruihong</creatorcontrib><creatorcontrib>Yang, Guangxiao</creatorcontrib><creatorcontrib>He, Guangyuan</creatorcontrib><title>A wheat WRKY transcription factor TaWRKY10 confers tolerance to multiple abiotic stresses in transgenic tobacco</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>WRKY transcription factors are reported to be involved in defense regulation, stress response and plant growth and development. However, the precise role of WRKY transcription factors in abiotic stress tolerance is not completely understood, especially in crops. In this study, we identified and cloned 10 WRKY genes from genome of wheat (Triticum aestivum L.). TaWRKY10, a gene induced by multiple stresses, was selected for further investigation. TaWRKY10 was upregulated by treatment with polyethylene glycol, NaCl, cold and H2O2. Result of Southern blot indicates that the wheat genome contains three copies of TaWRKY10. The TaWRKY10 protein is localized in the nucleus and functions as a transcriptional activator. Overexpression of TaWRKY10 in tobacco (Nicotiana tabacum L.) resulted in enhanced drought and salt stress tolerance, mainly demonstrated by the transgenic plants exhibiting of increased germination rate, root length, survival rate, and relative water content under these stress conditions. Further investigation showed that transgenic plants also retained higher proline and soluble sugar contents, and lower reactive oxygen species and malonaldehyde contents. Moreover, overexpression of the TaWRKY10 regulated the expression of a series of stress related genes. Taken together, our results indicate that TaWRKY10 functions as a positive factor under drought and salt stresses by regulating the osmotic balance, ROS scavenging and transcription of stress related genes.</description><subject>Abiotic factors</subject><subject>Abiotic stress</subject><subject>Adaptation, Physiological</subject><subject>Agriculture</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Arabidopsis</subject><subject>Barley</subject><subject>Biology</subject><subject>Biophysics</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>Cloning, Molecular</subject><subject>Cold</subject><subject>Cold Temperature</subject><subject>Cold treatment</subject><subject>DNA binding proteins</subject><subject>Drought</subject><subject>Droughts</subject><subject>Education</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetically modified plants</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Germination</subject><subject>Germination - physiology</subject><subject>Glycine max</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Immunoenzyme Techniques</subject><subject>Immunoglobulin G - immunology</subject><subject>International cooperation</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Life sciences</subject><subject>Malonaldehyde</subject><subject>Malondialdehyde</subject><subject>Moisture content</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - growth & development</subject><subject>Nicotiana - metabolism</subject><subject>Nuclei</subject><subject>Oxidants - pharmacology</subject><subject>Oxygen</subject><subject>Physiology</subject><subject>Plant growth</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - immunology</subject><subject>Plant Proteins - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Polyethylene</subject><subject>Polyethylene glycol</subject><subject>Proline</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Salinity tolerance</subject><subject>Salt Tolerance</subject><subject>Salts</subject><subject>Signal transduction</subject><subject>Sodium chloride</subject><subject>Sodium Chloride - pharmacology</subject><subject>Stress</subject><subject>Stress, Physiological</subject><subject>Stresses</subject><subject>Subcellular Fractions</subject><subject>Sugar</subject><subject>Survival</subject><subject>Tobacco</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcriptional Activation</subject><subject>Transgenic plants</subject><subject>Trends</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Triticum aestivum</subject><subject>Water content</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkmuL1DAUhoso7rr6D0QDguCHGXNrkn5ZGBYvgwsL66r4KaTp6UyGTjMmqZd_b8bpLlNQkAZ6OHnOm5OTtyieEjwnTJLXGz-E3nTzne9hjrEoCcX3ilNSMToTFLP7R_FJ8SjGDcYlU0I8LE4ok4LSqjwt_AL9WINJ6Mv1h68oBdNHG9wuOd-j1tjkA7ox-z2CkfV9CyGi5DvIoIUcoe3QJbfrAJna-eQsiilAjBCR6w96K-hzOvnaWOsfFw9a00V4Mv7Pik9v39xcvJ9dXr1bXiwuZ1ZgiWe1EUpRpQTBCte8VKIyUErO25pIUVOhKk5pg5koacm5wrRSrTGNaZjNIWZnxfOD7q7zUY-zipowIfKSRGVieSAabzZ6F9zWhF_aG6f_JHxYaRPyhTrQebqMiqZhDTRcVrSyFoTAjPCqLVsJWet8PG2ot9BY6PPNu4nodKd3a73y3zUTUqpq38yLUSD4bwPE9I-WR2plcleub30Ws1sXrV5wqRhnQtFMzf9C5a-BrctvCK3L-UnBq0lBZhL8TCszxKiXH6__n736PGVfHrHZZV1aR98Ne3PFKcgPoA0-xgDt3eQI1nuz305D782uR7PnsmfHU78runU3-w32tviq</recordid><startdate>20130610</startdate><enddate>20130610</enddate><creator>Wang, 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wheat WRKY transcription factor TaWRKY10 confers tolerance to multiple abiotic stresses in transgenic tobacco</title><author>Wang, Chen ; Deng, Pengyi ; Chen, Liulin ; Wang, Xiatian ; Ma, Hui ; Hu, Wei ; Yao, Ningcong ; Feng, Ying ; Chai, Ruihong ; Yang, Guangxiao ; He, Guangyuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6070-ba68828861080b45869ae5744fb176b2689422d0365254480298faadad3c29803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Abiotic factors</topic><topic>Abiotic stress</topic><topic>Adaptation, Physiological</topic><topic>Agriculture</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Arabidopsis</topic><topic>Barley</topic><topic>Biology</topic><topic>Biophysics</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>Cloning, Molecular</topic><topic>Cold</topic><topic>Cold Temperature</topic><topic>Cold treatment</topic><topic>DNA binding proteins</topic><topic>Drought</topic><topic>Droughts</topic><topic>Education</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetically modified plants</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Germination</topic><topic>Germination - physiology</topic><topic>Glycine max</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Immunoenzyme Techniques</topic><topic>Immunoglobulin G - immunology</topic><topic>International cooperation</topic><topic>Kinases</topic><topic>Laboratories</topic><topic>Life sciences</topic><topic>Malonaldehyde</topic><topic>Malondialdehyde</topic><topic>Moisture content</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - growth & development</topic><topic>Nicotiana - metabolism</topic><topic>Nuclei</topic><topic>Oxidants - pharmacology</topic><topic>Oxygen</topic><topic>Physiology</topic><topic>Plant 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Guangyuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A wheat WRKY transcription factor TaWRKY10 confers tolerance to multiple abiotic stresses in transgenic tobacco</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-06-10</date><risdate>2013</risdate><volume>8</volume><issue>6</issue><spage>e65120</spage><pages>e65120-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>WRKY transcription factors are reported to be involved in defense regulation, stress response and plant growth and development. However, the precise role of WRKY transcription factors in abiotic stress tolerance is not completely understood, especially in crops. In this study, we identified and cloned 10 WRKY genes from genome of wheat (Triticum aestivum L.). TaWRKY10, a gene induced by multiple stresses, was selected for further investigation. TaWRKY10 was upregulated by treatment with polyethylene glycol, NaCl, cold and H2O2. Result of Southern blot indicates that the wheat genome contains three copies of TaWRKY10. The TaWRKY10 protein is localized in the nucleus and functions as a transcriptional activator. Overexpression of TaWRKY10 in tobacco (Nicotiana tabacum L.) resulted in enhanced drought and salt stress tolerance, mainly demonstrated by the transgenic plants exhibiting of increased germination rate, root length, survival rate, and relative water content under these stress conditions. Further investigation showed that transgenic plants also retained higher proline and soluble sugar contents, and lower reactive oxygen species and malonaldehyde contents. Moreover, overexpression of the TaWRKY10 regulated the expression of a series of stress related genes. Taken together, our results indicate that TaWRKY10 functions as a positive factor under drought and salt stresses by regulating the osmotic balance, ROS scavenging and transcription of stress related genes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23762295</pmid><doi>10.1371/journal.pone.0065120</doi><tpages>e65120</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-06, Vol.8 (6), p.e65120 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1366366718 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abiotic factors Abiotic stress Adaptation, Physiological Agriculture Amino acids Animals Arabidopsis Barley Biology Biophysics Blotting, Southern Blotting, Western Cloning, Molecular Cold Cold Temperature Cold treatment DNA binding proteins Drought Droughts Education Gene expression Genes Genetic engineering Genetically modified plants Genomes Genomics Germination Germination - physiology Glycine max Hydrogen peroxide Hydrogen Peroxide - pharmacology Immunoenzyme Techniques Immunoglobulin G - immunology International cooperation Kinases Laboratories Life sciences Malonaldehyde Malondialdehyde Moisture content Nicotiana - genetics Nicotiana - growth & development Nicotiana - metabolism Nuclei Oxidants - pharmacology Oxygen Physiology Plant growth Plant Proteins - genetics Plant Proteins - immunology Plant Proteins - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Polyethylene Polyethylene glycol Proline Proteins Rabbits Reactive oxygen species Reactive Oxygen Species - metabolism Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Salinity tolerance Salt Tolerance Salts Signal transduction Sodium chloride Sodium Chloride - pharmacology Stress Stress, Physiological Stresses Subcellular Fractions Sugar Survival Tobacco Transcription factors Transcription Factors - genetics Transcription Factors - metabolism Transcriptional Activation Transgenic plants Trends Triticum - genetics Triticum - metabolism Triticum aestivum Water content Wheat |
| title | A wheat WRKY transcription factor TaWRKY10 confers tolerance to multiple abiotic stresses in transgenic tobacco |
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