Differential response of the cynomolgus macaque gut microbiota to Shigella infection
Little is known about the role of gut microbiota in response to live oral vaccines against enteric pathogens. We examined the effect of immunization with an oral live-attenuated Shigella dysenteriae 1 vaccine and challenge with wild-type S. dysenteriae 1 on the fecal microbiota of cynomolgus macaque...
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| Veröffentlicht in: | PloS one 2013-06, Vol.8 (6), p.e64212-e64212 |
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| creator | Seekatz, Anna M Panda, Aruna Rasko, David A Toapanta, Franklin R Eloe-Fadrosh, Emiley A Khan, Abdul Q Liu, Zhenqiu Shipley, Steven T Detolla, Louis J Sztein, Marcelo B Fraser, Claire M |
| description | Little is known about the role of gut microbiota in response to live oral vaccines against enteric pathogens. We examined the effect of immunization with an oral live-attenuated Shigella dysenteriae 1 vaccine and challenge with wild-type S. dysenteriae 1 on the fecal microbiota of cynomolgus macaques using 16 S rRNA analysis of fecal samples. Multi-dimensional cluster analysis identified different bacterial community types within macaques from geographically distinct locations. The fecal microbiota of Mauritian macaques, observed to be genetically distinct, harbored a high-diversity community and responded differently to Shigella immunization, as well as challenge compared to the microbiota in non-Mauritian macaques. While both macaque populations exhibited anti-Shigella antibody responses, clinical shigellosis was observed only among non-Mauritian macaques. These studies highlight the importance of further investigation into the possible protective role of the microbiota against enteric pathogens and consideration of host genetic backgrounds in conducting vaccine studies. |
| doi_str_mv | 10.1371/journal.pone.0064212 |
| format | Article |
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We examined the effect of immunization with an oral live-attenuated Shigella dysenteriae 1 vaccine and challenge with wild-type S. dysenteriae 1 on the fecal microbiota of cynomolgus macaques using 16 S rRNA analysis of fecal samples. Multi-dimensional cluster analysis identified different bacterial community types within macaques from geographically distinct locations. The fecal microbiota of Mauritian macaques, observed to be genetically distinct, harbored a high-diversity community and responded differently to Shigella immunization, as well as challenge compared to the microbiota in non-Mauritian macaques. While both macaque populations exhibited anti-Shigella antibody responses, clinical shigellosis was observed only among non-Mauritian macaques. These studies highlight the importance of further investigation into the possible protective role of the microbiota against enteric pathogens and consideration of host genetic backgrounds in conducting vaccine studies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0064212</identifier><identifier>PMID: 23755118</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Administration, Oral ; Animals ; Antibodies ; Antibodies, Bacterial - blood ; Bacteria ; Biology ; Cluster analysis ; Communities ; Comparative analysis ; Dimensional analysis ; Dysentery, Bacillary - microbiology ; Dysentery, Bacillary - prevention & control ; Epidemiology ; Fecal microflora ; Feces ; Feces - microbiology ; Female ; Gastrointestinal Tract - microbiology ; Genetic Variation ; Genomes ; Haplotypes ; Health aspects ; Host-Pathogen Interactions ; Immunization ; Immunology ; Infections ; Intestinal microflora ; Macaca fascicularis ; Macaca fascicularis - microbiology ; Male ; Medicine ; Microbiota ; Microbiota (Symbiotic organisms) ; Microbiota - genetics ; Molecular Typing ; Pathogens ; Pathology ; Phylogeny ; Public health ; RNA ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; rRNA ; Shigella ; Shigella dysenteriae - immunology ; Shigella dysenteriae - physiology ; Shigella Vaccines - administration & dosage ; Shigellosis ; Studies ; Vaccination ; Vaccines ; Vaccines, Attenuated - administration & dosage</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e64212-e64212</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013. This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. 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We examined the effect of immunization with an oral live-attenuated Shigella dysenteriae 1 vaccine and challenge with wild-type S. dysenteriae 1 on the fecal microbiota of cynomolgus macaques using 16 S rRNA analysis of fecal samples. Multi-dimensional cluster analysis identified different bacterial community types within macaques from geographically distinct locations. The fecal microbiota of Mauritian macaques, observed to be genetically distinct, harbored a high-diversity community and responded differently to Shigella immunization, as well as challenge compared to the microbiota in non-Mauritian macaques. While both macaque populations exhibited anti-Shigella antibody responses, clinical shigellosis was observed only among non-Mauritian macaques. 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We examined the effect of immunization with an oral live-attenuated Shigella dysenteriae 1 vaccine and challenge with wild-type S. dysenteriae 1 on the fecal microbiota of cynomolgus macaques using 16 S rRNA analysis of fecal samples. Multi-dimensional cluster analysis identified different bacterial community types within macaques from geographically distinct locations. The fecal microbiota of Mauritian macaques, observed to be genetically distinct, harbored a high-diversity community and responded differently to Shigella immunization, as well as challenge compared to the microbiota in non-Mauritian macaques. While both macaque populations exhibited anti-Shigella antibody responses, clinical shigellosis was observed only among non-Mauritian macaques. These studies highlight the importance of further investigation into the possible protective role of the microbiota against enteric pathogens and consideration of host genetic backgrounds in conducting vaccine studies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23755118</pmid><doi>10.1371/journal.pone.0064212</doi><tpages>e64212</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-06, Vol.8 (6), p.e64212-e64212 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1365188026 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Administration, Oral Animals Antibodies Antibodies, Bacterial - blood Bacteria Biology Cluster analysis Communities Comparative analysis Dimensional analysis Dysentery, Bacillary - microbiology Dysentery, Bacillary - prevention & control Epidemiology Fecal microflora Feces Feces - microbiology Female Gastrointestinal Tract - microbiology Genetic Variation Genomes Haplotypes Health aspects Host-Pathogen Interactions Immunization Immunology Infections Intestinal microflora Macaca fascicularis Macaca fascicularis - microbiology Male Medicine Microbiota Microbiota (Symbiotic organisms) Microbiota - genetics Molecular Typing Pathogens Pathology Phylogeny Public health RNA RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics rRNA Shigella Shigella dysenteriae - immunology Shigella dysenteriae - physiology Shigella Vaccines - administration & dosage Shigellosis Studies Vaccination Vaccines Vaccines, Attenuated - administration & dosage |
| title | Differential response of the cynomolgus macaque gut microbiota to Shigella infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T15%3A34%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20response%20of%20the%20cynomolgus%20macaque%20gut%20microbiota%20to%20Shigella%20infection&rft.jtitle=PloS%20one&rft.au=Seekatz,%20Anna%20M&rft.date=2013-06-05&rft.volume=8&rft.issue=6&rft.spage=e64212&rft.epage=e64212&rft.pages=e64212-e64212&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0064212&rft_dat=%3Cgale_plos_%3EA478417216%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1365188026&rft_id=info:pmid/23755118&rft_galeid=A478417216&rft_doaj_id=oai_doaj_org_article_f161a02a8af64477bfa0c961f19a8d5d&rfr_iscdi=true |