tmRNA is essential in Shigella flexneri
Nonstop mRNAs pose a challenge for bacteria, because translation cannot terminate efficiently without a stop codon. The trans-translation pathway resolves nonstop translation complexes by removing the nonstop mRNA, the incomplete protein, and the stalled ribosome. P1 co-transduction experiments demo...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-02, Vol.8 (2), p.e57537-e57537 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e57537 |
|---|---|
| container_issue | 2 |
| container_start_page | e57537 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Ramadoss, Nitya S Zhou, Xin Keiler, Kenneth C |
| description | Nonstop mRNAs pose a challenge for bacteria, because translation cannot terminate efficiently without a stop codon. The trans-translation pathway resolves nonstop translation complexes by removing the nonstop mRNA, the incomplete protein, and the stalled ribosome. P1 co-transduction experiments demonstrated that tmRNA, a key component of the trans-translation pathway, is essential for viability in Shigella flexneri. tmRNA was previously shown to be dispensable in the closely related species Escherichia coli, because E. coli contains a backup system for trans-translation mediated by the alternative release factor ArfA. Genome sequence analysis showed that S. flexneri does not have a gene encoding ArfA. E. coli ArfA could suppress the requirement for tmRNA in S. flexneri, indicating that tmRNA is essential in S. flexneri because there is no functional backup system. These data suggest that resolution of nonstop translation complexes is required for most bacteria. |
| doi_str_mv | 10.1371/journal.pone.0057537 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1351902110</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478882235</galeid><doaj_id>oai_doaj_org_article_2f35ceb8ca664aa4a7ad4b74067270d9</doaj_id><sourcerecordid>A478882235</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-2f4a54d8359dfe7f31598507d2fd6b895a3812f6e9472bbfe0fe3c770ae525ef3</originalsourceid><addsrcrecordid>eNqNkl2L1DAUhoso7rr6D0QLgh8XM-Y77Y0wLH4MLC7sqrchbU86GTLNmLSy_ntTp7tMZS8kFwknz3lPzsmbZc8xWmIq8futH0Kn3XLvO1gixCWn8kF2iktKFoIg-vDofJI9iXGbIFoI8Tg7IZRxTBg6zd70u6uvq9zGHGKErrfa5bbLrze2Bed0bhzcdBDs0-yR0S7Cs2k_y75_-vjt_Mvi4vLz-nx1sahFSfoFMUxz1hSUl40BaSjmZcGRbIhpRFWUXNMCEyOgZJJUlQFkgNZSIg2ccDD0LHt50N07H9XUY1SYclwigjFKxPpANF5v1T7YnQ6_lddW_Q340Codels7UMRQXkNV1FoIpjXTUjeskgwJSSRqyqT1Yao2VDto6jSAoN1MdH7T2Y1q_S9FeYGZkEng7SQQ_M8BYq92Ntbj5Drww_huzCgV6W8S-uof9P7uJqrVqQHbGZ_q1qOoWjFZFAUhlCdqeQ-VVgM7WydDGJvis4R3s4TE9HDTt3qIUa2vr_6fvfwxZ18fsRvQrt9E74be-i7OQXYA6-BjDGDuhoyRGv18Ow01-llNfk5pL44_6C7p1sD0D-k07Y4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1351902110</pqid></control><display><type>article</type><title>tmRNA is essential in Shigella flexneri</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Ramadoss, Nitya S ; Zhou, Xin ; Keiler, Kenneth C</creator><contributor>Herman, Christophe</contributor><creatorcontrib>Ramadoss, Nitya S ; Zhou, Xin ; Keiler, Kenneth C ; Herman, Christophe</creatorcontrib><description>Nonstop mRNAs pose a challenge for bacteria, because translation cannot terminate efficiently without a stop codon. The trans-translation pathway resolves nonstop translation complexes by removing the nonstop mRNA, the incomplete protein, and the stalled ribosome. P1 co-transduction experiments demonstrated that tmRNA, a key component of the trans-translation pathway, is essential for viability in Shigella flexneri. tmRNA was previously shown to be dispensable in the closely related species Escherichia coli, because E. coli contains a backup system for trans-translation mediated by the alternative release factor ArfA. Genome sequence analysis showed that S. flexneri does not have a gene encoding ArfA. E. coli ArfA could suppress the requirement for tmRNA in S. flexneri, indicating that tmRNA is essential in S. flexneri because there is no functional backup system. These data suggest that resolution of nonstop translation complexes is required for most bacteria.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0057537</identifier><identifier>PMID: 23451240</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Bacteria ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Biochemistry ; Biology ; Cloning ; Codon, Terminator ; Codons ; Deoxyribonucleic acid ; DNA ; E coli ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Genes ; Genetic engineering ; Genomes ; Genomics ; Laboratories ; Medicine ; Messenger RNA ; Molecular biology ; mRNA ; Nucleotide sequence ; Polypeptides ; Protein Biosynthesis ; Proteins ; RNA, Bacterial - genetics ; Shigella ; Shigella flexneri - genetics ; Shigella flexneri - metabolism ; Stop codon ; TmRNA ; Translation ; Viability</subject><ispartof>PloS one, 2013-02, Vol.8 (2), p.e57537-e57537</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Ramadoss et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Ramadoss et al 2013 Ramadoss et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-2f4a54d8359dfe7f31598507d2fd6b895a3812f6e9472bbfe0fe3c770ae525ef3</citedby><cites>FETCH-LOGICAL-c692t-2f4a54d8359dfe7f31598507d2fd6b895a3812f6e9472bbfe0fe3c770ae525ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581467/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581467/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23451240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Herman, Christophe</contributor><creatorcontrib>Ramadoss, Nitya S</creatorcontrib><creatorcontrib>Zhou, Xin</creatorcontrib><creatorcontrib>Keiler, Kenneth C</creatorcontrib><title>tmRNA is essential in Shigella flexneri</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Nonstop mRNAs pose a challenge for bacteria, because translation cannot terminate efficiently without a stop codon. The trans-translation pathway resolves nonstop translation complexes by removing the nonstop mRNA, the incomplete protein, and the stalled ribosome. P1 co-transduction experiments demonstrated that tmRNA, a key component of the trans-translation pathway, is essential for viability in Shigella flexneri. tmRNA was previously shown to be dispensable in the closely related species Escherichia coli, because E. coli contains a backup system for trans-translation mediated by the alternative release factor ArfA. Genome sequence analysis showed that S. flexneri does not have a gene encoding ArfA. E. coli ArfA could suppress the requirement for tmRNA in S. flexneri, indicating that tmRNA is essential in S. flexneri because there is no functional backup system. These data suggest that resolution of nonstop translation complexes is required for most bacteria.</description><subject>Bacteria</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - genetics</subject><subject>Biochemistry</subject><subject>Biology</subject><subject>Cloning</subject><subject>Codon, Terminator</subject><subject>Codons</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Laboratories</subject><subject>Medicine</subject><subject>Messenger RNA</subject><subject>Molecular biology</subject><subject>mRNA</subject><subject>Nucleotide sequence</subject><subject>Polypeptides</subject><subject>Protein Biosynthesis</subject><subject>Proteins</subject><subject>RNA, Bacterial - genetics</subject><subject>Shigella</subject><subject>Shigella flexneri - genetics</subject><subject>Shigella flexneri - metabolism</subject><subject>Stop codon</subject><subject>TmRNA</subject><subject>Translation</subject><subject>Viability</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLgh8XM-Y77Y0wLH4MLC7sqrchbU86GTLNmLSy_ntTp7tMZS8kFwknz3lPzsmbZc8xWmIq8futH0Kn3XLvO1gixCWn8kF2iktKFoIg-vDofJI9iXGbIFoI8Tg7IZRxTBg6zd70u6uvq9zGHGKErrfa5bbLrze2Bed0bhzcdBDs0-yR0S7Cs2k_y75_-vjt_Mvi4vLz-nx1sahFSfoFMUxz1hSUl40BaSjmZcGRbIhpRFWUXNMCEyOgZJJUlQFkgNZSIg2ccDD0LHt50N07H9XUY1SYclwigjFKxPpANF5v1T7YnQ6_lddW_Q340Codels7UMRQXkNV1FoIpjXTUjeskgwJSSRqyqT1Yao2VDto6jSAoN1MdH7T2Y1q_S9FeYGZkEng7SQQ_M8BYq92Ntbj5Drww_huzCgV6W8S-uof9P7uJqrVqQHbGZ_q1qOoWjFZFAUhlCdqeQ-VVgM7WydDGJvis4R3s4TE9HDTt3qIUa2vr_6fvfwxZ18fsRvQrt9E74be-i7OQXYA6-BjDGDuhoyRGv18Ow01-llNfk5pL44_6C7p1sD0D-k07Y4</recordid><startdate>20130225</startdate><enddate>20130225</enddate><creator>Ramadoss, Nitya S</creator><creator>Zhou, Xin</creator><creator>Keiler, Kenneth C</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130225</creationdate><title>tmRNA is essential in Shigella flexneri</title><author>Ramadoss, Nitya S ; Zhou, Xin ; Keiler, Kenneth C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-2f4a54d8359dfe7f31598507d2fd6b895a3812f6e9472bbfe0fe3c770ae525ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Bacteria</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Biochemistry</topic><topic>Biology</topic><topic>Cloning</topic><topic>Codon, Terminator</topic><topic>Codons</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Laboratories</topic><topic>Medicine</topic><topic>Messenger RNA</topic><topic>Molecular biology</topic><topic>mRNA</topic><topic>Nucleotide sequence</topic><topic>Polypeptides</topic><topic>Protein Biosynthesis</topic><topic>Proteins</topic><topic>RNA, Bacterial - genetics</topic><topic>Shigella</topic><topic>Shigella flexneri - genetics</topic><topic>Shigella flexneri - metabolism</topic><topic>Stop codon</topic><topic>TmRNA</topic><topic>Translation</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramadoss, Nitya S</creatorcontrib><creatorcontrib>Zhou, Xin</creatorcontrib><creatorcontrib>Keiler, Kenneth C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramadoss, Nitya S</au><au>Zhou, Xin</au><au>Keiler, Kenneth C</au><au>Herman, Christophe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>tmRNA is essential in Shigella flexneri</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-02-25</date><risdate>2013</risdate><volume>8</volume><issue>2</issue><spage>e57537</spage><epage>e57537</epage><pages>e57537-e57537</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Nonstop mRNAs pose a challenge for bacteria, because translation cannot terminate efficiently without a stop codon. The trans-translation pathway resolves nonstop translation complexes by removing the nonstop mRNA, the incomplete protein, and the stalled ribosome. P1 co-transduction experiments demonstrated that tmRNA, a key component of the trans-translation pathway, is essential for viability in Shigella flexneri. tmRNA was previously shown to be dispensable in the closely related species Escherichia coli, because E. coli contains a backup system for trans-translation mediated by the alternative release factor ArfA. Genome sequence analysis showed that S. flexneri does not have a gene encoding ArfA. E. coli ArfA could suppress the requirement for tmRNA in S. flexneri, indicating that tmRNA is essential in S. flexneri because there is no functional backup system. These data suggest that resolution of nonstop translation complexes is required for most bacteria.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23451240</pmid><doi>10.1371/journal.pone.0057537</doi><tpages>e57537</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-02, Vol.8 (2), p.e57537-e57537 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1351902110 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Biochemistry Biology Cloning Codon, Terminator Codons Deoxyribonucleic acid DNA E coli Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Genes Genetic engineering Genomes Genomics Laboratories Medicine Messenger RNA Molecular biology mRNA Nucleotide sequence Polypeptides Protein Biosynthesis Proteins RNA, Bacterial - genetics Shigella Shigella flexneri - genetics Shigella flexneri - metabolism Stop codon TmRNA Translation Viability |
| title | tmRNA is essential in Shigella flexneri |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T13%3A41%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=tmRNA%20is%20essential%20in%20Shigella%20flexneri&rft.jtitle=PloS%20one&rft.au=Ramadoss,%20Nitya%20S&rft.date=2013-02-25&rft.volume=8&rft.issue=2&rft.spage=e57537&rft.epage=e57537&rft.pages=e57537-e57537&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0057537&rft_dat=%3Cgale_plos_%3EA478882235%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1351902110&rft_id=info:pmid/23451240&rft_galeid=A478882235&rft_doaj_id=oai_doaj_org_article_2f35ceb8ca664aa4a7ad4b74067270d9&rfr_iscdi=true |