Ocular mucosal CD11b+ and CD103+ mouse dendritic cells under normal conditions and in allergic immune responses
Steady state dendritic cells (DC) found in non-lymphoid tissue sites under normal physiologic conditions play a pivotal role in triggering T cell responses upon immune provocation. CD11b+ and CD103+ DC have received considerable attention in this regard. However, still unknown is whether such CD11b+...
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| description | Steady state dendritic cells (DC) found in non-lymphoid tissue sites under normal physiologic conditions play a pivotal role in triggering T cell responses upon immune provocation. CD11b+ and CD103+ DC have received considerable attention in this regard. However, still unknown is whether such CD11b+ and CD103+ DC even exist in the ocular mucosa, and if so, what functions they have in shaping immune responses. We herein identified in the ocular mucosa of normal wild-type (WT) and Flt3-/- mice the presence of a CD11b+ DC (i.e., CD11c+ MHCII+ CD11b+ CD103- F4/80+ Sirp-a+). CD103+ DC (i.e. CD11c+ MHCII+ CD11b low CD103+ CD8a+ DEC205+ Langerin+) were also present in WT, but not in Flt3-/- mice. These CD103+ DC expressed high levels of Id2 and Flt3 mRNA; whereas CD11b+ DC expressed high Irf4, Csfr, and Cx3cr1 mRNA. Additionally, the functions of these DC differed in response to allergic immune provocation. This was assessed utilizing a previously validated model, which includes transferring specific populations of exogenous DC into the ocular mucosa of ovalbumin (OVA)/alum-primed mice. Interestingly, in such mice, topical OVA instillation following engraftment of exogenous CD11b+ DC led to dominant allergic T cell responses and clinical signs of ocular allergy relative to those engrafted with CD103+ DC. Thus, although CD11b+ and CD103+ DC are both present in the normal ocular mucosa, the CD11b+ DC subset plays a dominant role in a mouse model of ocular allergy. |
| doi_str_mv | 10.1371/journal.pone.0064193 |
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CD11b+ and CD103+ DC have received considerable attention in this regard. However, still unknown is whether such CD11b+ and CD103+ DC even exist in the ocular mucosa, and if so, what functions they have in shaping immune responses. We herein identified in the ocular mucosa of normal wild-type (WT) and Flt3-/- mice the presence of a CD11b+ DC (i.e., CD11c+ MHCII+ CD11b+ CD103- F4/80+ Sirp-a+). CD103+ DC (i.e. CD11c+ MHCII+ CD11b low CD103+ CD8a+ DEC205+ Langerin+) were also present in WT, but not in Flt3-/- mice. These CD103+ DC expressed high levels of Id2 and Flt3 mRNA; whereas CD11b+ DC expressed high Irf4, Csfr, and Cx3cr1 mRNA. Additionally, the functions of these DC differed in response to allergic immune provocation. This was assessed utilizing a previously validated model, which includes transferring specific populations of exogenous DC into the ocular mucosa of ovalbumin (OVA)/alum-primed mice. Interestingly, in such mice, topical OVA instillation following engraftment of exogenous CD11b+ DC led to dominant allergic T cell responses and clinical signs of ocular allergy relative to those engrafted with CD103+ DC. Thus, although CD11b+ and CD103+ DC are both present in the normal ocular mucosa, the CD11b+ DC subset plays a dominant role in a mouse model of ocular allergy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0064193</identifier><identifier>PMID: 23691170</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adaptive Immunity ; Allergies ; Alum ; Aluminum sulfate ; Analysis ; Animals ; Antigens ; Antigens, CD - metabolism ; Biology ; CD103 antigen ; CD11b antigen ; CD11b Antigen - metabolism ; CD11c antigen ; CX3CR1 protein ; Dendritic cells ; Dendritic Cells - immunology ; Dendritic Cells - metabolism ; Eye - immunology ; Female ; fms-Like Tyrosine Kinase 3 - deficiency ; fms-Like Tyrosine Kinase 3 - genetics ; Gene Knockout Techniques ; Hypersensitivity ; Hypersensitivity - immunology ; Immune response ; Integrin alpha Chains - metabolism ; Interferon regulatory factor 4 ; Liver ; Lymphocytes T ; Lymphoid tissue ; Mathematical models ; Medical schools ; Medicine ; Mice ; Mice, Inbred C57BL ; mRNA ; Mucosa ; Mucous Membrane - immunology ; Ovalbumin ; Phenotype ; Rodents ; Studies ; T cell receptors ; T cells ; Transcription, Genetic</subject><ispartof>PloS one, 2013-05, Vol.8 (5), p.e64193-e64193</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Khandelwal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Khandelwal et al 2013 Khandelwal et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a141815d8c041f1f4ae715f3bb8cdf0e522e87a3939bef2f920ba886401bc71b3</citedby><cites>FETCH-LOGICAL-c692t-a141815d8c041f1f4ae715f3bb8cdf0e522e87a3939bef2f920ba886401bc71b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653857/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653857/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23691170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khandelwal, Payal</creatorcontrib><creatorcontrib>Blanco-Mezquita, Tomas</creatorcontrib><creatorcontrib>Emami, Parisa</creatorcontrib><creatorcontrib>Lee, Hyun Soo</creatorcontrib><creatorcontrib>Reyes, Nancy J</creatorcontrib><creatorcontrib>Mathew, Rose</creatorcontrib><creatorcontrib>Huang, Randy</creatorcontrib><creatorcontrib>Saban, Daniel R</creatorcontrib><title>Ocular mucosal CD11b+ and CD103+ mouse dendritic cells under normal conditions and in allergic immune responses</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Steady state dendritic cells (DC) found in non-lymphoid tissue sites under normal physiologic conditions play a pivotal role in triggering T cell responses upon immune provocation. CD11b+ and CD103+ DC have received considerable attention in this regard. However, still unknown is whether such CD11b+ and CD103+ DC even exist in the ocular mucosa, and if so, what functions they have in shaping immune responses. We herein identified in the ocular mucosa of normal wild-type (WT) and Flt3-/- mice the presence of a CD11b+ DC (i.e., CD11c+ MHCII+ CD11b+ CD103- F4/80+ Sirp-a+). CD103+ DC (i.e. CD11c+ MHCII+ CD11b low CD103+ CD8a+ DEC205+ Langerin+) were also present in WT, but not in Flt3-/- mice. These CD103+ DC expressed high levels of Id2 and Flt3 mRNA; whereas CD11b+ DC expressed high Irf4, Csfr, and Cx3cr1 mRNA. Additionally, the functions of these DC differed in response to allergic immune provocation. This was assessed utilizing a previously validated model, which includes transferring specific populations of exogenous DC into the ocular mucosa of ovalbumin (OVA)/alum-primed mice. Interestingly, in such mice, topical OVA instillation following engraftment of exogenous CD11b+ DC led to dominant allergic T cell responses and clinical signs of ocular allergy relative to those engrafted with CD103+ DC. Thus, although CD11b+ and CD103+ DC are both present in the normal ocular mucosa, the CD11b+ DC subset plays a dominant role in a mouse model of ocular allergy.</description><subject>Adaptive Immunity</subject><subject>Allergies</subject><subject>Alum</subject><subject>Aluminum sulfate</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antigens</subject><subject>Antigens, CD - metabolism</subject><subject>Biology</subject><subject>CD103 antigen</subject><subject>CD11b antigen</subject><subject>CD11b Antigen - metabolism</subject><subject>CD11c antigen</subject><subject>CX3CR1 protein</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - metabolism</subject><subject>Eye - immunology</subject><subject>Female</subject><subject>fms-Like Tyrosine Kinase 3 - deficiency</subject><subject>fms-Like Tyrosine Kinase 3 - genetics</subject><subject>Gene Knockout Techniques</subject><subject>Hypersensitivity</subject><subject>Hypersensitivity - immunology</subject><subject>Immune response</subject><subject>Integrin alpha Chains - metabolism</subject><subject>Interferon regulatory factor 4</subject><subject>Liver</subject><subject>Lymphocytes T</subject><subject>Lymphoid tissue</subject><subject>Mathematical models</subject><subject>Medical schools</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>mRNA</subject><subject>Mucosa</subject><subject>Mucous Membrane - immunology</subject><subject>Ovalbumin</subject><subject>Phenotype</subject><subject>Rodents</subject><subject>Studies</subject><subject>T cell receptors</subject><subject>T cells</subject><subject>Transcription, Genetic</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk2uL1DAUhoso7jr6D0QLgijLjLm0SfpFWMbbwsKAt68hTU5nOqTJbNKK_nvTme4yI_tBCklInvc9OSc9WfYcowWmHL_b-iE4ZRc772CBECtwRR9k52kkc0YQfXi0PsuexLhFqKSCscfZGaGswpij88yv9GBVyLtB-6hsvvyAcX2RK2fGJaIXeeeHCLkBZ0LbtzrXYG3MB2cg5M6HLom0dyadeRf3wtblyloI60S3XTc4yAPEdM8I8Wn2qFE2wrNpnmU_Pn38vvwyv159vlpeXs81q0g_V7jAApdGaFTgBjeFAo7Lhta10KZBUBICgita0aqGhjQVQbUSghUI15rjms6ylwffnfVRTrWKEtMS04JVBUvE1YEwXm3lLrSdCn-kV63cb_iwliqkhC1IkuIYwUpRFrxAVAsjSKUNNowhjjRKXu-naEPdgdHg-qDsienpiWs3cu1_ScrSk5Q8GbyZDIK_GSD2smvjWGnlINV_vDetBCdpmmWv_kHvz26i1iol0LrGp7h6NJWXBReEcyRGr8U9VPoMdG16VWjatH8ieHsiSEwPv_u1GmKUV9--_j-7-nnKvj5iN6Bsv4neDvuf6hQsDqAOPsYAzV2RMZJjX9xWQ459Iae-SLIXxw90J7ptBPoXUuYGOQ</recordid><startdate>20130514</startdate><enddate>20130514</enddate><creator>Khandelwal, Payal</creator><creator>Blanco-Mezquita, Tomas</creator><creator>Emami, Parisa</creator><creator>Lee, Hyun Soo</creator><creator>Reyes, Nancy J</creator><creator>Mathew, Rose</creator><creator>Huang, Randy</creator><creator>Saban, Daniel R</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130514</creationdate><title>Ocular mucosal CD11b+ and CD103+ mouse dendritic cells under normal conditions and in allergic immune responses</title><author>Khandelwal, Payal ; Blanco-Mezquita, Tomas ; Emami, Parisa ; Lee, Hyun Soo ; Reyes, Nancy J ; Mathew, Rose ; Huang, Randy ; Saban, Daniel R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-a141815d8c041f1f4ae715f3bb8cdf0e522e87a3939bef2f920ba886401bc71b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adaptive Immunity</topic><topic>Allergies</topic><topic>Alum</topic><topic>Aluminum sulfate</topic><topic>Analysis</topic><topic>Animals</topic><topic>Antigens</topic><topic>Antigens, CD - 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CD11b+ and CD103+ DC have received considerable attention in this regard. However, still unknown is whether such CD11b+ and CD103+ DC even exist in the ocular mucosa, and if so, what functions they have in shaping immune responses. We herein identified in the ocular mucosa of normal wild-type (WT) and Flt3-/- mice the presence of a CD11b+ DC (i.e., CD11c+ MHCII+ CD11b+ CD103- F4/80+ Sirp-a+). CD103+ DC (i.e. CD11c+ MHCII+ CD11b low CD103+ CD8a+ DEC205+ Langerin+) were also present in WT, but not in Flt3-/- mice. These CD103+ DC expressed high levels of Id2 and Flt3 mRNA; whereas CD11b+ DC expressed high Irf4, Csfr, and Cx3cr1 mRNA. Additionally, the functions of these DC differed in response to allergic immune provocation. This was assessed utilizing a previously validated model, which includes transferring specific populations of exogenous DC into the ocular mucosa of ovalbumin (OVA)/alum-primed mice. Interestingly, in such mice, topical OVA instillation following engraftment of exogenous CD11b+ DC led to dominant allergic T cell responses and clinical signs of ocular allergy relative to those engrafted with CD103+ DC. Thus, although CD11b+ and CD103+ DC are both present in the normal ocular mucosa, the CD11b+ DC subset plays a dominant role in a mouse model of ocular allergy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23691170</pmid><doi>10.1371/journal.pone.0064193</doi><tpages>e64193</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-05, Vol.8 (5), p.e64193-e64193 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| subjects | Adaptive Immunity Allergies Alum Aluminum sulfate Analysis Animals Antigens Antigens, CD - metabolism Biology CD103 antigen CD11b antigen CD11b Antigen - metabolism CD11c antigen CX3CR1 protein Dendritic cells Dendritic Cells - immunology Dendritic Cells - metabolism Eye - immunology Female fms-Like Tyrosine Kinase 3 - deficiency fms-Like Tyrosine Kinase 3 - genetics Gene Knockout Techniques Hypersensitivity Hypersensitivity - immunology Immune response Integrin alpha Chains - metabolism Interferon regulatory factor 4 Liver Lymphocytes T Lymphoid tissue Mathematical models Medical schools Medicine Mice Mice, Inbred C57BL mRNA Mucosa Mucous Membrane - immunology Ovalbumin Phenotype Rodents Studies T cell receptors T cells Transcription, Genetic |
| title | Ocular mucosal CD11b+ and CD103+ mouse dendritic cells under normal conditions and in allergic immune responses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T07%3A45%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ocular%20mucosal%20CD11b+%20and%20CD103+%20mouse%20dendritic%20cells%20under%20normal%20conditions%20and%20in%20allergic%20immune%20responses&rft.jtitle=PloS%20one&rft.au=Khandelwal,%20Payal&rft.date=2013-05-14&rft.volume=8&rft.issue=5&rft.spage=e64193&rft.epage=e64193&rft.pages=e64193-e64193&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0064193&rft_dat=%3Cgale_plos_%3EA478277085%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1351346946&rft_id=info:pmid/23691170&rft_galeid=A478277085&rft_doaj_id=oai_doaj_org_article_2393d8658547403c8d829cd1d66070c0&rfr_iscdi=true |