Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda
Identification of pathogens associated with bovine mastitis is helpful in treatment and management decisions. However, such data from sub-Saharan Africa is scarce. Here we describe the distribution and antimicrobial susceptibility patterns of bacteria from cows with clinical mastitis in Kampala, Uga...
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| Veröffentlicht in: | PloS one 2013-05, Vol.8 (5), p.e63413-e63413 |
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| creator | Kateete, David Patrick Kabugo, Usuf Baluku, Hannington Nyakarahuka, Luke Kyobe, Samuel Okee, Moses Najjuka, Christine Florence Joloba, Moses Lutaakome |
| description | Identification of pathogens associated with bovine mastitis is helpful in treatment and management decisions. However, such data from sub-Saharan Africa is scarce. Here we describe the distribution and antimicrobial susceptibility patterns of bacteria from cows with clinical mastitis in Kampala, Uganda. Due to high concern of zoonotic infections, isolates from milkmen are also described.
Ninety seven milk samples from cows with clinical mastitis and 31 nasal swabs from milkmen were collected (one sample per cow/human). Fifty eight (60%) Gram-positive isolates namely Staphylococci (21), Enterococci (16), Streptococci (13), Lactococci (5), Micrococci (2) and Arcanobacteria (1) were detected in cows; only one grew Staphylococcus aureus. Furthermore, 24 (25%) coliforms namely Escherichia coli (12), Klebsiella oxytoca (5), Proteus vulgaris (2), Serratia (2), Citrobacter (1), Cedecea (1) and Leclercia (1) were identified. From humans, 24 Gram-positive bacteria grew, of which 11 were Staphylococci (35%) including four Staphylococcus aureus. Upon susceptibility testing, methicillin-resistant coagulase-negative staphylococci (CoNS) were prevalent; 57%, 12/21 in cows and 64%, 7/11 in humans. However, methicillin-resistant Staphylococcus aureus was not detected. Furthermore, methicillin and vancomycin resistant CoNS were detected in cows (Staphylococcus hominis, Staphylococcus lugdunensis) and humans (Staphylococcus scuiri). Also, vancomycin and daptomycin resistant Enterococci (Enterococcus faecalis and Enterococcus faecium, respectively) were detected in cows. Coliforms were less resistant with three pan-susceptible isolates. However, multidrug resistant Klebsiella, Proteus, Serratia, Cedecea, and Citrobacter were detected. Lastly, similar species grew from human and bovine samples but on genotyping, the isolates were found to be different. Interestingly, human and bovine Staphylococcus aureus were genetically similar (spa-CC435, spa-type t645 corresponding to ST121) but with different susceptibility patterns.
CoNS, Enterococci, Streptococci, and Escherichia coli are the predominant pathogens associated with clinical bovine-mastitis in Kampala, Uganda. Multidrug resistant bacteria are also prevalent. While similar species occurred in humans and cows, transmission was not detected. |
| doi_str_mv | 10.1371/journal.pone.0063413 |
| format | Article |
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Ninety seven milk samples from cows with clinical mastitis and 31 nasal swabs from milkmen were collected (one sample per cow/human). Fifty eight (60%) Gram-positive isolates namely Staphylococci (21), Enterococci (16), Streptococci (13), Lactococci (5), Micrococci (2) and Arcanobacteria (1) were detected in cows; only one grew Staphylococcus aureus. Furthermore, 24 (25%) coliforms namely Escherichia coli (12), Klebsiella oxytoca (5), Proteus vulgaris (2), Serratia (2), Citrobacter (1), Cedecea (1) and Leclercia (1) were identified. From humans, 24 Gram-positive bacteria grew, of which 11 were Staphylococci (35%) including four Staphylococcus aureus. Upon susceptibility testing, methicillin-resistant coagulase-negative staphylococci (CoNS) were prevalent; 57%, 12/21 in cows and 64%, 7/11 in humans. However, methicillin-resistant Staphylococcus aureus was not detected. Furthermore, methicillin and vancomycin resistant CoNS were detected in cows (Staphylococcus hominis, Staphylococcus lugdunensis) and humans (Staphylococcus scuiri). Also, vancomycin and daptomycin resistant Enterococci (Enterococcus faecalis and Enterococcus faecium, respectively) were detected in cows. Coliforms were less resistant with three pan-susceptible isolates. However, multidrug resistant Klebsiella, Proteus, Serratia, Cedecea, and Citrobacter were detected. Lastly, similar species grew from human and bovine samples but on genotyping, the isolates were found to be different. Interestingly, human and bovine Staphylococcus aureus were genetically similar (spa-CC435, spa-type t645 corresponding to ST121) but with different susceptibility patterns.
CoNS, Enterococci, Streptococci, and Escherichia coli are the predominant pathogens associated with clinical bovine-mastitis in Kampala, Uganda. Multidrug resistant bacteria are also prevalent. While similar species occurred in humans and cows, transmission was not detected.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0063413</identifier><identifier>PMID: 23667611</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animal Technicians ; Animals ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - therapeutic use ; Antibacterial agents ; Antiinfectives and antibacterials ; Antimicrobial agents ; Bacteria ; Bacteria - drug effects ; Bovidae ; Cattle ; Citrobacter ; Coagulase ; Coliforms ; Cow's milk ; Dairy cattle ; Dairy industry ; Daptomycin ; Disease ; Disease transmission ; Drug resistance ; Drug Resistance, Microbial - drug effects ; E coli ; Enterococcus - drug effects ; Enterococcus - isolation & purification ; Epidemiology ; Escherichia coli ; Female ; Genotyping ; Gram-positive bacteria ; Health aspects ; Health sciences ; Humans ; Identification ; Infection ; Klebsiella ; Mammary Glands, Animal - microbiology ; Mammary Glands, Animal - pathology ; Mastitis ; Mastitis, Bovine - drug therapy ; Mastitis, Bovine - epidemiology ; Mastitis, Bovine - microbiology ; Medicine ; Methicillin ; Microbial Sensitivity Tests ; Milk ; Milk - microbiology ; Multidrug resistance ; Pathogens ; Phylogeny ; Prevalence ; Proteus vulgaris ; Random Amplified Polymorphic DNA Technique ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - isolation & purification ; Staphylococcus aureus infections ; Staphylococcus infections ; Streptococcus ; Uganda - epidemiology ; Vancomycin ; Veterinary colleges ; Veterinary medicine ; Veterinary Science ; Zoonoses</subject><ispartof>PloS one, 2013-05, Vol.8 (5), p.e63413-e63413</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Kateete et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Kateete et al 2013 Kateete et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ad87637238f6c04eab0f08ebb332868a480ddf473730c966681c4b942abfcb953</citedby><cites>FETCH-LOGICAL-c692t-ad87637238f6c04eab0f08ebb332868a480ddf473730c966681c4b942abfcb953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646745/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3646745/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23667611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gordon, Stephen V.</contributor><creatorcontrib>Kateete, David Patrick</creatorcontrib><creatorcontrib>Kabugo, Usuf</creatorcontrib><creatorcontrib>Baluku, Hannington</creatorcontrib><creatorcontrib>Nyakarahuka, Luke</creatorcontrib><creatorcontrib>Kyobe, Samuel</creatorcontrib><creatorcontrib>Okee, Moses</creatorcontrib><creatorcontrib>Najjuka, Christine Florence</creatorcontrib><creatorcontrib>Joloba, Moses Lutaakome</creatorcontrib><title>Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Identification of pathogens associated with bovine mastitis is helpful in treatment and management decisions. However, such data from sub-Saharan Africa is scarce. Here we describe the distribution and antimicrobial susceptibility patterns of bacteria from cows with clinical mastitis in Kampala, Uganda. Due to high concern of zoonotic infections, isolates from milkmen are also described.
Ninety seven milk samples from cows with clinical mastitis and 31 nasal swabs from milkmen were collected (one sample per cow/human). Fifty eight (60%) Gram-positive isolates namely Staphylococci (21), Enterococci (16), Streptococci (13), Lactococci (5), Micrococci (2) and Arcanobacteria (1) were detected in cows; only one grew Staphylococcus aureus. Furthermore, 24 (25%) coliforms namely Escherichia coli (12), Klebsiella oxytoca (5), Proteus vulgaris (2), Serratia (2), Citrobacter (1), Cedecea (1) and Leclercia (1) were identified. From humans, 24 Gram-positive bacteria grew, of which 11 were Staphylococci (35%) including four Staphylococcus aureus. Upon susceptibility testing, methicillin-resistant coagulase-negative staphylococci (CoNS) were prevalent; 57%, 12/21 in cows and 64%, 7/11 in humans. However, methicillin-resistant Staphylococcus aureus was not detected. Furthermore, methicillin and vancomycin resistant CoNS were detected in cows (Staphylococcus hominis, Staphylococcus lugdunensis) and humans (Staphylococcus scuiri). Also, vancomycin and daptomycin resistant Enterococci (Enterococcus faecalis and Enterococcus faecium, respectively) were detected in cows. Coliforms were less resistant with three pan-susceptible isolates. However, multidrug resistant Klebsiella, Proteus, Serratia, Cedecea, and Citrobacter were detected. Lastly, similar species grew from human and bovine samples but on genotyping, the isolates were found to be different. Interestingly, human and bovine Staphylococcus aureus were genetically similar (spa-CC435, spa-type t645 corresponding to ST121) but with different susceptibility patterns.
CoNS, Enterococci, Streptococci, and Escherichia coli are the predominant pathogens associated with clinical bovine-mastitis in Kampala, Uganda. Multidrug resistant bacteria are also prevalent. While similar species occurred in humans and cows, transmission was not detected.</description><subject>Animal Technicians</subject><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Antibacterial agents</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Bacteria - drug effects</subject><subject>Bovidae</subject><subject>Cattle</subject><subject>Citrobacter</subject><subject>Coagulase</subject><subject>Coliforms</subject><subject>Cow's milk</subject><subject>Dairy cattle</subject><subject>Dairy industry</subject><subject>Daptomycin</subject><subject>Disease</subject><subject>Disease transmission</subject><subject>Drug resistance</subject><subject>Drug Resistance, Microbial - drug effects</subject><subject>E coli</subject><subject>Enterococcus - drug effects</subject><subject>Enterococcus - isolation & purification</subject><subject>Epidemiology</subject><subject>Escherichia coli</subject><subject>Female</subject><subject>Genotyping</subject><subject>Gram-positive bacteria</subject><subject>Health aspects</subject><subject>Health sciences</subject><subject>Humans</subject><subject>Identification</subject><subject>Infection</subject><subject>Klebsiella</subject><subject>Mammary Glands, Animal - 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microbiology</topic><topic>Mammary Glands, Animal - pathology</topic><topic>Mastitis</topic><topic>Mastitis, Bovine - drug therapy</topic><topic>Mastitis, Bovine - epidemiology</topic><topic>Mastitis, Bovine - microbiology</topic><topic>Medicine</topic><topic>Methicillin</topic><topic>Microbial Sensitivity Tests</topic><topic>Milk</topic><topic>Milk - microbiology</topic><topic>Multidrug resistance</topic><topic>Pathogens</topic><topic>Phylogeny</topic><topic>Prevalence</topic><topic>Proteus vulgaris</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Staphylococcus aureus infections</topic><topic>Staphylococcus infections</topic><topic>Streptococcus</topic><topic>Uganda - epidemiology</topic><topic>Vancomycin</topic><topic>Veterinary colleges</topic><topic>Veterinary medicine</topic><topic>Veterinary Science</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kateete, David Patrick</creatorcontrib><creatorcontrib>Kabugo, Usuf</creatorcontrib><creatorcontrib>Baluku, Hannington</creatorcontrib><creatorcontrib>Nyakarahuka, Luke</creatorcontrib><creatorcontrib>Kyobe, Samuel</creatorcontrib><creatorcontrib>Okee, Moses</creatorcontrib><creatorcontrib>Najjuka, Christine Florence</creatorcontrib><creatorcontrib>Joloba, Moses Lutaakome</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kateete, David Patrick</au><au>Kabugo, Usuf</au><au>Baluku, Hannington</au><au>Nyakarahuka, Luke</au><au>Kyobe, Samuel</au><au>Okee, Moses</au><au>Najjuka, Christine Florence</au><au>Joloba, Moses Lutaakome</au><au>Gordon, Stephen V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-05-07</date><risdate>2013</risdate><volume>8</volume><issue>5</issue><spage>e63413</spage><epage>e63413</epage><pages>e63413-e63413</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Identification of pathogens associated with bovine mastitis is helpful in treatment and management decisions. However, such data from sub-Saharan Africa is scarce. Here we describe the distribution and antimicrobial susceptibility patterns of bacteria from cows with clinical mastitis in Kampala, Uganda. Due to high concern of zoonotic infections, isolates from milkmen are also described.
Ninety seven milk samples from cows with clinical mastitis and 31 nasal swabs from milkmen were collected (one sample per cow/human). Fifty eight (60%) Gram-positive isolates namely Staphylococci (21), Enterococci (16), Streptococci (13), Lactococci (5), Micrococci (2) and Arcanobacteria (1) were detected in cows; only one grew Staphylococcus aureus. Furthermore, 24 (25%) coliforms namely Escherichia coli (12), Klebsiella oxytoca (5), Proteus vulgaris (2), Serratia (2), Citrobacter (1), Cedecea (1) and Leclercia (1) were identified. From humans, 24 Gram-positive bacteria grew, of which 11 were Staphylococci (35%) including four Staphylococcus aureus. Upon susceptibility testing, methicillin-resistant coagulase-negative staphylococci (CoNS) were prevalent; 57%, 12/21 in cows and 64%, 7/11 in humans. However, methicillin-resistant Staphylococcus aureus was not detected. Furthermore, methicillin and vancomycin resistant CoNS were detected in cows (Staphylococcus hominis, Staphylococcus lugdunensis) and humans (Staphylococcus scuiri). Also, vancomycin and daptomycin resistant Enterococci (Enterococcus faecalis and Enterococcus faecium, respectively) were detected in cows. Coliforms were less resistant with three pan-susceptible isolates. However, multidrug resistant Klebsiella, Proteus, Serratia, Cedecea, and Citrobacter were detected. Lastly, similar species grew from human and bovine samples but on genotyping, the isolates were found to be different. Interestingly, human and bovine Staphylococcus aureus were genetically similar (spa-CC435, spa-type t645 corresponding to ST121) but with different susceptibility patterns.
CoNS, Enterococci, Streptococci, and Escherichia coli are the predominant pathogens associated with clinical bovine-mastitis in Kampala, Uganda. Multidrug resistant bacteria are also prevalent. While similar species occurred in humans and cows, transmission was not detected.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23667611</pmid><doi>10.1371/journal.pone.0063413</doi><tpages>e63413</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-05, Vol.8 (5), p.e63413-e63413 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1350968626 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animal Technicians Animals Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Antibacterial agents Antiinfectives and antibacterials Antimicrobial agents Bacteria Bacteria - drug effects Bovidae Cattle Citrobacter Coagulase Coliforms Cow's milk Dairy cattle Dairy industry Daptomycin Disease Disease transmission Drug resistance Drug Resistance, Microbial - drug effects E coli Enterococcus - drug effects Enterococcus - isolation & purification Epidemiology Escherichia coli Female Genotyping Gram-positive bacteria Health aspects Health sciences Humans Identification Infection Klebsiella Mammary Glands, Animal - microbiology Mammary Glands, Animal - pathology Mastitis Mastitis, Bovine - drug therapy Mastitis, Bovine - epidemiology Mastitis, Bovine - microbiology Medicine Methicillin Microbial Sensitivity Tests Milk Milk - microbiology Multidrug resistance Pathogens Phylogeny Prevalence Proteus vulgaris Random Amplified Polymorphic DNA Technique Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - isolation & purification Staphylococcus aureus infections Staphylococcus infections Streptococcus Uganda - epidemiology Vancomycin Veterinary colleges Veterinary medicine Veterinary Science Zoonoses |
| title | Prevalence and antimicrobial susceptibility patterns of bacteria from milkmen and cows with clinical mastitis in and around Kampala, Uganda |
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