Multiple-level regulation of 2,4-diacetylphloroglucinol production by the sigma regulator PsrA in Pseudomonas fluorescens 2P24
Pseudomonas fluorescens 2P24 is a rhizospheric bacterium that aggressively colonizes the plant roots. It produces the antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG), which contributes to the protection of various crop plants against soil borne diseases caused by bacterial and fungal pathogens. The...
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| description | Pseudomonas fluorescens 2P24 is a rhizospheric bacterium that aggressively colonizes the plant roots. It produces the antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG), which contributes to the protection of various crop plants against soil borne diseases caused by bacterial and fungal pathogens. The biosynthesis of 2,4-DAPG is regulated at the transcriptional level in the expression of the phlACBD operon as well as at the posttranscriptional level by the Gac/Rsm signal transduction pathway. However, the detailed mechanism of such regulation is not clear.
In this study, we identified a binding site for the sigma regulator PsrA in the promoter region of the phlA gene. Electrophoretic mobility shift experiments revealed direct and specific binding of PsrA to the phlA promoter region. Consistent with the fact that its binding site locates within the promoter region of phlA, PsrA negatively regulates phlA expression, and its inactivation led to significant increase in 2,4-DAPG production. Interestingly, PsrA also activates the expression of the sigma factor RpoS, which negatively regulates 2,4-DAPG production by inducing the expression of the RNA-binding protein RsmA.
These results suggest that PsrA is an important regulator that modulates 2,4-DAPG biosynthesis at both transcriptional and posttranscriptional levels. |
| doi_str_mv | 10.1371/journal.pone.0050149 |
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In this study, we identified a binding site for the sigma regulator PsrA in the promoter region of the phlA gene. Electrophoretic mobility shift experiments revealed direct and specific binding of PsrA to the phlA promoter region. Consistent with the fact that its binding site locates within the promoter region of phlA, PsrA negatively regulates phlA expression, and its inactivation led to significant increase in 2,4-DAPG production. Interestingly, PsrA also activates the expression of the sigma factor RpoS, which negatively regulates 2,4-DAPG production by inducing the expression of the RNA-binding protein RsmA.
These results suggest that PsrA is an important regulator that modulates 2,4-DAPG biosynthesis at both transcriptional and posttranscriptional levels.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0050149</identifier><identifier>PMID: 23209661</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibiotics ; Bacteria ; Bacterial Proteins - metabolism ; Base Sequence ; beta-Galactosidase - metabolism ; Binding Sites ; Biology ; Biosynthesis ; Cellular signal transduction ; Chromosomes ; Cloning ; Deactivation ; Deoxyribonucleic acid ; DNA ; DNA-Binding Proteins - metabolism ; E coli ; Electrophoretic mobility ; Enzymes ; Gene expression ; Gene Expression Regulation ; Gene Expression Regulation, Bacterial ; Genes, Reporter ; Inactivation ; Kinases ; Metabolites ; Models, Genetic ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Mutation ; Operon ; Pathogens ; PhlA gene ; Phloroglucinol - analogs & derivatives ; Phloroglucinol - pharmacology ; Physiological aspects ; Plant diseases ; Plant pathology ; Plant protection ; Plant roots ; Plasmids ; Post-transcription ; Promoter Regions, Genetic ; Protein binding ; Proteins ; Pseudomonas ; Pseudomonas aeruginosa ; Pseudomonas fluorescens ; Pseudomonas fluorescens - genetics ; Pseudomonas fluorescens - metabolism ; Ribonucleic acid ; RNA ; RNA Processing, Post-Transcriptional ; RNA-binding protein ; Sigma factor ; Sigma Factor - genetics ; Sigma Factor - metabolism ; Signal Transduction ; Soil Microbiology ; Soil-borne diseases ; Transcription Factors - metabolism ; Transcription, Genetic ; Transduction</subject><ispartof>PloS one, 2012-11, Vol.7 (11), p.e50149</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Wu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Wu et al 2012 Wu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-bdc2b092b7d543f1e55293255224e593d96a80b3edeeda475ba78c1853e459f73</citedby><cites>FETCH-LOGICAL-c692t-bdc2b092b7d543f1e55293255224e593d96a80b3edeeda475ba78c1853e459f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510223/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3510223/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23209661$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Aziz, Ramy K.</contributor><creatorcontrib>Wu, Xiaogang</creatorcontrib><creatorcontrib>Liu, Jiucheng</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Zhang, Liqun</creatorcontrib><title>Multiple-level regulation of 2,4-diacetylphloroglucinol production by the sigma regulator PsrA in Pseudomonas fluorescens 2P24</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Pseudomonas fluorescens 2P24 is a rhizospheric bacterium that aggressively colonizes the plant roots. It produces the antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG), which contributes to the protection of various crop plants against soil borne diseases caused by bacterial and fungal pathogens. The biosynthesis of 2,4-DAPG is regulated at the transcriptional level in the expression of the phlACBD operon as well as at the posttranscriptional level by the Gac/Rsm signal transduction pathway. However, the detailed mechanism of such regulation is not clear.
In this study, we identified a binding site for the sigma regulator PsrA in the promoter region of the phlA gene. Electrophoretic mobility shift experiments revealed direct and specific binding of PsrA to the phlA promoter region. Consistent with the fact that its binding site locates within the promoter region of phlA, PsrA negatively regulates phlA expression, and its inactivation led to significant increase in 2,4-DAPG production. Interestingly, PsrA also activates the expression of the sigma factor RpoS, which negatively regulates 2,4-DAPG production by inducing the expression of the RNA-binding protein RsmA.
These results suggest that PsrA is an important regulator that modulates 2,4-DAPG biosynthesis at both transcriptional and posttranscriptional levels.</description><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>beta-Galactosidase - metabolism</subject><subject>Binding Sites</subject><subject>Biology</subject><subject>Biosynthesis</subject><subject>Cellular signal transduction</subject><subject>Chromosomes</subject><subject>Cloning</subject><subject>Deactivation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>E coli</subject><subject>Electrophoretic mobility</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Reporter</subject><subject>Inactivation</subject><subject>Kinases</subject><subject>Metabolites</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Site-Directed</subject><subject>Mutation</subject><subject>Operon</subject><subject>Pathogens</subject><subject>PhlA gene</subject><subject>Phloroglucinol - analogs & derivatives</subject><subject>Phloroglucinol - pharmacology</subject><subject>Physiological aspects</subject><subject>Plant diseases</subject><subject>Plant pathology</subject><subject>Plant protection</subject><subject>Plant roots</subject><subject>Plasmids</subject><subject>Post-transcription</subject><subject>Promoter Regions, Genetic</subject><subject>Protein binding</subject><subject>Proteins</subject><subject>Pseudomonas</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas fluorescens</subject><subject>Pseudomonas fluorescens - genetics</subject><subject>Pseudomonas fluorescens - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Processing, Post-Transcriptional</subject><subject>RNA-binding protein</subject><subject>Sigma factor</subject><subject>Sigma Factor - genetics</subject><subject>Sigma Factor - metabolism</subject><subject>Signal Transduction</subject><subject>Soil Microbiology</subject><subject>Soil-borne diseases</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transduction</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkmuL1DAUhoso7rr6D0QLgiDYMbe2ky_CsHgZWNnF29eQJqedLGnTTdrF-eJvN7PTWaagIIEknDznzTmHN0meY7TAtMTvrt3oO2kXvetggVCOMOMPklPMKckKgujDo_tJ8iSE6wjRZVE8Tk4IJYgXBT5Nfn8Z7WB6C5mFW7Cph2a0cjCuS12dkrcs00YqGLa231jnXWNHZTpn0947Pao7sNqmwwbSYJpWHgScT6-CX6WmiyeM2rWukyGt7eg8BAVdSMkVYU-TR7W0AZ5N51ny4-OH7-efs4vLT-vz1UWmCk6GrNKKVIiTqtQ5ozWGPCext7gTBjmnmhdyiSoKGkBLVuaVLJcKL3MKLOd1Sc-Sl3vd3rogptEFgWmOOMasWEZivSe0k9ei96aVfiucNOIu4HwjpB-MsiAI1zXlTGnghMW6KqJAlaQmspAVqXZa76ffxqoFHbsdvLQz0flLZzaicbeC5hgRQqPAq0nAu5sRwvCPkieqkbEq09UuiqnWBCVWrCwRYqhgkVr8hYpLQ2tUNE9tYnyW8GaWEJkBfg2NHEMQ629f_5-9_DlnXx-xG5B22ARnx52Hwhxke1B5F4KH-n5yGImd9w_TEDvvi8n7Me3F8dTvkw5mp38AkO4Aew</recordid><startdate>20121129</startdate><enddate>20121129</enddate><creator>Wu, Xiaogang</creator><creator>Liu, Jiucheng</creator><creator>Zhang, Wei</creator><creator>Zhang, Liqun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20121129</creationdate><title>Multiple-level regulation of 2,4-diacetylphloroglucinol production by the sigma regulator PsrA in Pseudomonas fluorescens 2P24</title><author>Wu, Xiaogang ; Liu, Jiucheng ; Zhang, Wei ; Zhang, Liqun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-bdc2b092b7d543f1e55293255224e593d96a80b3edeeda475ba78c1853e459f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial Proteins - metabolism</topic><topic>Base Sequence</topic><topic>beta-Galactosidase - metabolism</topic><topic>Binding Sites</topic><topic>Biology</topic><topic>Biosynthesis</topic><topic>Cellular signal transduction</topic><topic>Chromosomes</topic><topic>Cloning</topic><topic>Deactivation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>E coli</topic><topic>Electrophoretic mobility</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Reporter</topic><topic>Inactivation</topic><topic>Kinases</topic><topic>Metabolites</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Site-Directed</topic><topic>Mutation</topic><topic>Operon</topic><topic>Pathogens</topic><topic>PhlA gene</topic><topic>Phloroglucinol - analogs & derivatives</topic><topic>Phloroglucinol - pharmacology</topic><topic>Physiological aspects</topic><topic>Plant diseases</topic><topic>Plant pathology</topic><topic>Plant protection</topic><topic>Plant roots</topic><topic>Plasmids</topic><topic>Post-transcription</topic><topic>Promoter Regions, Genetic</topic><topic>Protein binding</topic><topic>Proteins</topic><topic>Pseudomonas</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas fluorescens</topic><topic>Pseudomonas fluorescens - genetics</topic><topic>Pseudomonas fluorescens - metabolism</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Processing, Post-Transcriptional</topic><topic>RNA-binding protein</topic><topic>Sigma factor</topic><topic>Sigma Factor - genetics</topic><topic>Sigma Factor - metabolism</topic><topic>Signal Transduction</topic><topic>Soil Microbiology</topic><topic>Soil-borne diseases</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Xiaogang</creatorcontrib><creatorcontrib>Liu, Jiucheng</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Zhang, Liqun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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It produces the antibiotic 2,4-diacetylphoroglucinol (2,4-DAPG), which contributes to the protection of various crop plants against soil borne diseases caused by bacterial and fungal pathogens. The biosynthesis of 2,4-DAPG is regulated at the transcriptional level in the expression of the phlACBD operon as well as at the posttranscriptional level by the Gac/Rsm signal transduction pathway. However, the detailed mechanism of such regulation is not clear.
In this study, we identified a binding site for the sigma regulator PsrA in the promoter region of the phlA gene. Electrophoretic mobility shift experiments revealed direct and specific binding of PsrA to the phlA promoter region. Consistent with the fact that its binding site locates within the promoter region of phlA, PsrA negatively regulates phlA expression, and its inactivation led to significant increase in 2,4-DAPG production. Interestingly, PsrA also activates the expression of the sigma factor RpoS, which negatively regulates 2,4-DAPG production by inducing the expression of the RNA-binding protein RsmA.
These results suggest that PsrA is an important regulator that modulates 2,4-DAPG biosynthesis at both transcriptional and posttranscriptional levels.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23209661</pmid><doi>10.1371/journal.pone.0050149</doi><tpages>e50149</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antibiotics Bacteria Bacterial Proteins - metabolism Base Sequence beta-Galactosidase - metabolism Binding Sites Biology Biosynthesis Cellular signal transduction Chromosomes Cloning Deactivation Deoxyribonucleic acid DNA DNA-Binding Proteins - metabolism E coli Electrophoretic mobility Enzymes Gene expression Gene Expression Regulation Gene Expression Regulation, Bacterial Genes, Reporter Inactivation Kinases Metabolites Models, Genetic Molecular Sequence Data Mutagenesis, Site-Directed Mutation Operon Pathogens PhlA gene Phloroglucinol - analogs & derivatives Phloroglucinol - pharmacology Physiological aspects Plant diseases Plant pathology Plant protection Plant roots Plasmids Post-transcription Promoter Regions, Genetic Protein binding Proteins Pseudomonas Pseudomonas aeruginosa Pseudomonas fluorescens Pseudomonas fluorescens - genetics Pseudomonas fluorescens - metabolism Ribonucleic acid RNA RNA Processing, Post-Transcriptional RNA-binding protein Sigma factor Sigma Factor - genetics Sigma Factor - metabolism Signal Transduction Soil Microbiology Soil-borne diseases Transcription Factors - metabolism Transcription, Genetic Transduction |
| title | Multiple-level regulation of 2,4-diacetylphloroglucinol production by the sigma regulator PsrA in Pseudomonas fluorescens 2P24 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T10%3A07%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Multiple-level%20regulation%20of%202,4-diacetylphloroglucinol%20production%20by%20the%20sigma%20regulator%20PsrA%20in%20Pseudomonas%20fluorescens%202P24&rft.jtitle=PloS%20one&rft.au=Wu,%20Xiaogang&rft.date=2012-11-29&rft.volume=7&rft.issue=11&rft.spage=e50149&rft.pages=e50149-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0050149&rft_dat=%3Cgale_plos_%3EA477004064%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1350911468&rft_id=info:pmid/23209661&rft_galeid=A477004064&rft_doaj_id=oai_doaj_org_article_29df394cde9242b0b2cec72f2a6ab2b8&rfr_iscdi=true |