Simulation of between repeat variability in real time PCR reactions

While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process. Besides influences of more obvious factors such as camera noise and pipett...

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Veröffentlicht in:	PloS one 2012-11, Vol.7 (11), p.e47112-e47112
Hauptverfasser:	Lievens, Antoon, Van Aelst, Stefan, Van den Bulcke, Marc, Goetghebeur, Els
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Analysis Analysis of Variance Applied mathematics Bioinformatics Biology Computer science Computer Simulation Consumption Decision analysis Deoxyribonucleic acid DNA Efficiency Enzymes Error analysis Fluorescence Mathematical models Mathematics Methods Models, Statistical Molecular biology Parameter estimation Polymerase chain reaction Process parameters Public health Real time Real-Time Polymerase Chain Reaction - standards Reproducibility Reproducibility of Results Sensitivity and Specificity Side reactions Simulation Soybeans Statistical analysis Statistical models Stochasticity Variation
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