Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes
In adipose cells, adipose triglyceride lipase (ATGL) catalyzes the first step in adipocyte triacylglyceride hydrolysis, thereby regulating both basal and hormone-stimulated lipolysis. However, little is known about the molecular mechanism(s) underlying habitual exercise-induced adaptive modulation o...
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| creator | Ogasawara, Junetsu Sakurai, Takuya Kizaki, Takako Ishibashi, Yoshinaga Izawa, Tetsuya Sumitani, Yoshikazu Ishida, Hitoshi Radak, Zsolt Haga, Shukoh Ohno, Hideki |
| description | In adipose cells, adipose triglyceride lipase (ATGL) catalyzes the first step in adipocyte triacylglyceride hydrolysis, thereby regulating both basal and hormone-stimulated lipolysis. However, little is known about the molecular mechanism(s) underlying habitual exercise-induced adaptive modulation of ATGL in white adipocytes via alteration in transcription regulator and lipolytic cofactors.
Male Wistar rats were randomly divided into 2 groups a sedentary control group (CG) and a habitual exercise group (EG). The EG was subjected to running on a treadmill set at 5 days per week for 9 weeks. The CG was not subjected to running on a treadmill. In the EG, levels of ATGL mRNA and protein were elevated with a significant increase in lipolysis compared with the CG, accompanied by a significant increase in associations of CGI-58 with ATGL protein. Under these conditions, an upregulation of peroxisome proliferation-activated receptorg-2 (PPARg-2) was observed. In the EG, the addition of rosiglitazone further significantly increased the levels of ATGL protein compared with the CG. However, attenuated levels of the ATGL protein in adipocytes were obtained by the addition of insulin, which is known to inhibit the expression of ATGL, in both types of groups. Actually, levels of plasma insulin were significantly reduced in the EG compared with the CG.
These data suggest that elevated levels of ATGL are involved in the exercise-induced enhancement of lipolysis in primary adipocytes. The exact mechanism(s) underlying this phenomenon is associated, at least in part, with upregulated transcriptional activation of PPARg-2. In addition, exercise-induced lower circulation levels of insulin also correlate with habitual exercise-induced higher levels of ATGL in primary adipocytes. |
| doi_str_mv | 10.1371/journal.pone.0040876 |
| format | Article |
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Male Wistar rats were randomly divided into 2 groups a sedentary control group (CG) and a habitual exercise group (EG). The EG was subjected to running on a treadmill set at 5 days per week for 9 weeks. The CG was not subjected to running on a treadmill. In the EG, levels of ATGL mRNA and protein were elevated with a significant increase in lipolysis compared with the CG, accompanied by a significant increase in associations of CGI-58 with ATGL protein. Under these conditions, an upregulation of peroxisome proliferation-activated receptorg-2 (PPARg-2) was observed. In the EG, the addition of rosiglitazone further significantly increased the levels of ATGL protein compared with the CG. However, attenuated levels of the ATGL protein in adipocytes were obtained by the addition of insulin, which is known to inhibit the expression of ATGL, in both types of groups. Actually, levels of plasma insulin were significantly reduced in the EG compared with the CG.
These data suggest that elevated levels of ATGL are involved in the exercise-induced enhancement of lipolysis in primary adipocytes. The exact mechanism(s) underlying this phenomenon is associated, at least in part, with upregulated transcriptional activation of PPARg-2. In addition, exercise-induced lower circulation levels of insulin also correlate with habitual exercise-induced higher levels of ATGL in primary adipocytes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0040876</identifier><identifier>PMID: 22815850</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acyltransferases ; Adipocytes ; Adipocytes - drug effects ; Adipocytes - enzymology ; Animals ; Biology ; Body Weight - drug effects ; Carrier Proteins - metabolism ; Cofactors ; DNA - metabolism ; Epididymis - cytology ; Exercise ; Fatty acids ; Gene Expression Regulation, Enzymologic - drug effects ; Glycerol ; HeLa Cells ; Hormones ; Humans ; Hydrolysis ; Insulin ; Insulin - blood ; Insulin - pharmacology ; Insulin resistance ; Internal medicine ; Kinases ; Laboratory animals ; Lipase ; Lipase - genetics ; Lipase - metabolism ; Lipids ; Lipolysis ; Lipolysis - drug effects ; Male ; Medicine ; Metabolism ; Organ Size - drug effects ; Perilipin-1 ; Peroxisome proliferator-activated receptors ; Phosphoproteins - metabolism ; Physical Conditioning, Animal ; Physical training ; PPAR gamma - genetics ; PPAR gamma - metabolism ; Protein Binding - drug effects ; Proteins ; Rats ; Rats, Wistar ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Rosiglitazone ; Sport science ; Sterol Esterase - metabolism ; Thiazolidinediones - pharmacology ; Transcription activation ; Triglycerides</subject><ispartof>PloS one, 2012-07, Vol.7 (7), p.e40876</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Ogasawara et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Ogasawara et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-547057287b1864d697b8717921abff83739614a194a40851c5754009d24444be3</citedby><cites>FETCH-LOGICAL-c692t-547057287b1864d697b8717921abff83739614a194a40851c5754009d24444be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397928/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3397928/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22815850$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tomé, Daniel</contributor><creatorcontrib>Ogasawara, Junetsu</creatorcontrib><creatorcontrib>Sakurai, Takuya</creatorcontrib><creatorcontrib>Kizaki, Takako</creatorcontrib><creatorcontrib>Ishibashi, Yoshinaga</creatorcontrib><creatorcontrib>Izawa, Tetsuya</creatorcontrib><creatorcontrib>Sumitani, Yoshikazu</creatorcontrib><creatorcontrib>Ishida, Hitoshi</creatorcontrib><creatorcontrib>Radak, Zsolt</creatorcontrib><creatorcontrib>Haga, Shukoh</creatorcontrib><creatorcontrib>Ohno, Hideki</creatorcontrib><title>Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In adipose cells, adipose triglyceride lipase (ATGL) catalyzes the first step in adipocyte triacylglyceride hydrolysis, thereby regulating both basal and hormone-stimulated lipolysis. However, little is known about the molecular mechanism(s) underlying habitual exercise-induced adaptive modulation of ATGL in white adipocytes via alteration in transcription regulator and lipolytic cofactors.
Male Wistar rats were randomly divided into 2 groups a sedentary control group (CG) and a habitual exercise group (EG). The EG was subjected to running on a treadmill set at 5 days per week for 9 weeks. The CG was not subjected to running on a treadmill. In the EG, levels of ATGL mRNA and protein were elevated with a significant increase in lipolysis compared with the CG, accompanied by a significant increase in associations of CGI-58 with ATGL protein. Under these conditions, an upregulation of peroxisome proliferation-activated receptorg-2 (PPARg-2) was observed. In the EG, the addition of rosiglitazone further significantly increased the levels of ATGL protein compared with the CG. However, attenuated levels of the ATGL protein in adipocytes were obtained by the addition of insulin, which is known to inhibit the expression of ATGL, in both types of groups. Actually, levels of plasma insulin were significantly reduced in the EG compared with the CG.
These data suggest that elevated levels of ATGL are involved in the exercise-induced enhancement of lipolysis in primary adipocytes. The exact mechanism(s) underlying this phenomenon is associated, at least in part, with upregulated transcriptional activation of PPARg-2. In addition, exercise-induced lower circulation levels of insulin also correlate with habitual exercise-induced higher levels of ATGL in primary adipocytes.</description><subject>Acyltransferases</subject><subject>Adipocytes</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - enzymology</subject><subject>Animals</subject><subject>Biology</subject><subject>Body Weight - drug effects</subject><subject>Carrier Proteins - metabolism</subject><subject>Cofactors</subject><subject>DNA - metabolism</subject><subject>Epididymis - cytology</subject><subject>Exercise</subject><subject>Fatty acids</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>Glycerol</subject><subject>HeLa Cells</subject><subject>Hormones</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Insulin</subject><subject>Insulin - blood</subject><subject>Insulin - pharmacology</subject><subject>Insulin resistance</subject><subject>Internal medicine</subject><subject>Kinases</subject><subject>Laboratory animals</subject><subject>Lipase</subject><subject>Lipase - genetics</subject><subject>Lipase - metabolism</subject><subject>Lipids</subject><subject>Lipolysis</subject><subject>Lipolysis - drug effects</subject><subject>Male</subject><subject>Medicine</subject><subject>Metabolism</subject><subject>Organ Size - drug effects</subject><subject>Perilipin-1</subject><subject>Peroxisome proliferator-activated receptors</subject><subject>Phosphoproteins - metabolism</subject><subject>Physical Conditioning, Animal</subject><subject>Physical training</subject><subject>PPAR gamma - genetics</subject><subject>PPAR gamma - metabolism</subject><subject>Protein Binding - drug effects</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Rosiglitazone</subject><subject>Sport science</subject><subject>Sterol Esterase - metabolism</subject><subject>Thiazolidinediones - pharmacology</subject><subject>Transcription activation</subject><subject>Triglycerides</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl-L1DAUxYso7jr6DUQLguDDjPnXJn0RhkV3BwYWdPU1ZNLbaZa0GZN03fn2ZpzuMgUFm4eGm985SU5ulr3GaIEpxx9v3eB7ZRc718MCIYYEL59k57iiZF4SRJ-ezM-yFyHcIlRQUZbPszNCBC5Egc6z3ZXZtuBzC3dgQ-6afHlzuc6Vh1yF4LRREer8l4ltDvfgtQkwN3096FSFvlW9hg76eBBas3N2H0zITZ97FXPYmdrU-07ZXNVpUe8jhJfZs0bZAK_G_yz7_uXzzcXVfH19ubpYrue6rEicF4yjghPBN1iUrC4rvhEc84pgtWkaQTmtSswUrphKNy-wLnjBEKpqwtK3ATrL3h59d9YFOYYVJKZMYII5pYlYHYnaqVu586ZTfi-dMvJPwfmtVD4abUHWDaTNlNIggFHVVFoUokFUE46BMJK8Po27DZsOap0i8cpOTKcrvWnl1t1JSqt0KZEM3o0G3v0cIMR_HHmktiqdyvSNS2a6M0HLJeMccc7Sa8-yxV-oNGrojE7t0phUnwg-TASJiXAft2oIQa6-ff1_9vrHlH1_wragbGyDs0M0rg9TkB1B7V0IHprH5DCSh25_SEMeul2O3Z5kb05TfxQ9tDf9DY40-Z4</recordid><startdate>20120716</startdate><enddate>20120716</enddate><creator>Ogasawara, Junetsu</creator><creator>Sakurai, Takuya</creator><creator>Kizaki, Takako</creator><creator>Ishibashi, Yoshinaga</creator><creator>Izawa, Tetsuya</creator><creator>Sumitani, Yoshikazu</creator><creator>Ishida, Hitoshi</creator><creator>Radak, Zsolt</creator><creator>Haga, Shukoh</creator><creator>Ohno, Hideki</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120716</creationdate><title>Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes</title><author>Ogasawara, Junetsu ; Sakurai, Takuya ; Kizaki, Takako ; Ishibashi, Yoshinaga ; Izawa, Tetsuya ; Sumitani, Yoshikazu ; Ishida, Hitoshi ; Radak, Zsolt ; Haga, Shukoh ; Ohno, Hideki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-547057287b1864d697b8717921abff83739614a194a40851c5754009d24444be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acyltransferases</topic><topic>Adipocytes</topic><topic>Adipocytes - 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However, little is known about the molecular mechanism(s) underlying habitual exercise-induced adaptive modulation of ATGL in white adipocytes via alteration in transcription regulator and lipolytic cofactors.
Male Wistar rats were randomly divided into 2 groups a sedentary control group (CG) and a habitual exercise group (EG). The EG was subjected to running on a treadmill set at 5 days per week for 9 weeks. The CG was not subjected to running on a treadmill. In the EG, levels of ATGL mRNA and protein were elevated with a significant increase in lipolysis compared with the CG, accompanied by a significant increase in associations of CGI-58 with ATGL protein. Under these conditions, an upregulation of peroxisome proliferation-activated receptorg-2 (PPARg-2) was observed. In the EG, the addition of rosiglitazone further significantly increased the levels of ATGL protein compared with the CG. However, attenuated levels of the ATGL protein in adipocytes were obtained by the addition of insulin, which is known to inhibit the expression of ATGL, in both types of groups. Actually, levels of plasma insulin were significantly reduced in the EG compared with the CG.
These data suggest that elevated levels of ATGL are involved in the exercise-induced enhancement of lipolysis in primary adipocytes. The exact mechanism(s) underlying this phenomenon is associated, at least in part, with upregulated transcriptional activation of PPARg-2. In addition, exercise-induced lower circulation levels of insulin also correlate with habitual exercise-induced higher levels of ATGL in primary adipocytes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22815850</pmid><doi>10.1371/journal.pone.0040876</doi><tpages>e40876</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1348121733 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acyltransferases Adipocytes Adipocytes - drug effects Adipocytes - enzymology Animals Biology Body Weight - drug effects Carrier Proteins - metabolism Cofactors DNA - metabolism Epididymis - cytology Exercise Fatty acids Gene Expression Regulation, Enzymologic - drug effects Glycerol HeLa Cells Hormones Humans Hydrolysis Insulin Insulin - blood Insulin - pharmacology Insulin resistance Internal medicine Kinases Laboratory animals Lipase Lipase - genetics Lipase - metabolism Lipids Lipolysis Lipolysis - drug effects Male Medicine Metabolism Organ Size - drug effects Perilipin-1 Peroxisome proliferator-activated receptors Phosphoproteins - metabolism Physical Conditioning, Animal Physical training PPAR gamma - genetics PPAR gamma - metabolism Protein Binding - drug effects Proteins Rats Rats, Wistar RNA RNA, Messenger - genetics RNA, Messenger - metabolism Rosiglitazone Sport science Sterol Esterase - metabolism Thiazolidinediones - pharmacology Transcription activation Triglycerides |
| title | Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-11T23%3A54%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Higher%20levels%20of%20ATGL%20are%20associated%20with%20exercise-induced%20enhancement%20of%20lipolysis%20in%20rat%20epididymal%20adipocytes&rft.jtitle=PloS%20one&rft.au=Ogasawara,%20Junetsu&rft.date=2012-07-16&rft.volume=7&rft.issue=7&rft.spage=e40876&rft.pages=e40876-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0040876&rft_dat=%3Cgale_plos_%3EA477077420%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1348121733&rft_id=info:pmid/22815850&rft_galeid=A477077420&rft_doaj_id=oai_doaj_org_article_dfe14aaace8e43af9c858f03c271e242&rfr_iscdi=true |