Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue fever
Little is known about the role of chemokines/chemokines receptors on T cells in natural DENV infection. Patients from DENV-2 and -3- outbreaks were studied prospectively during the acute or convalescent phases. Expression of chemokine receptor and activation markers on lymphocyte subpopulations were...
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| Veröffentlicht in: | PloS one 2012-07, Vol.7 (7), p.e38527-e38527 |
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| creator | de-Oliveira-Pinto, Luzia Maria Marinho, Cíntia Ferreira Povoa, Tiago Fajardo de Azeredo, Elzinandes Leal de Souza, Luiza Assed Barbosa, Luiza Damian Ribeiro Motta-Castro, Ana Rita C Alves, Ada M B Ávila, Carlos André Lins de Souza, Luiz José da Cunha, Rivaldo Venâncio Damasco, Paulo Vieira Paes, Marciano Viana Kubelka, Claire Fernandes |
| description | Little is known about the role of chemokines/chemokines receptors on T cells in natural DENV infection. Patients from DENV-2 and -3- outbreaks were studied prospectively during the acute or convalescent phases. Expression of chemokine receptor and activation markers on lymphocyte subpopulations were determined by flow cytometry analysis, plasma chemokine ligands concentrations were measured by ELISA and quantification of CCL5/RANTES(+) cells in liver tissues from fatal dengue cases was performed by immunochemistry. In the acute DENV-infection, T-helper/T-cytotoxic type-1 cell (Th1/Tc1)-related CCR5 is significantly higher expressed on both CD4 and CD8 T cells. The Th1-related CXCR3 is up-regulated among CD4 T cells and Tc2-related CCR4 is up-regulated among CD8 T cells. In the convalescent phase, all chemokine receptor or chemokine ligand expression tends to reestablish control healthy levels. Increased CCL2/MCP-1 and CCL4/MIP-1β but decreased CCL5/RANTES levels were observed in DENV-patients during acute infection. Moreover, we showed an increased CD107a expression on CCR5 or CXCR3-expressing T cells and higher expression of CD29, CD44(HIGH) and CD127(LOW) markers on CCR4-expressing CD8 T cells in DENV-patients when compared to controls. Finally, liver from dengue fatal patients showed increased number of cells expressing CCL5/RANTES in three out of four cases compared to three death from a non-dengue patient. In conclusion, both Th1-related CCR5 and CXCR3 among CD4 T cells have a potential ability to exert cytotoxicity function. Moreover, Tc1-related CCR5 and Tc2-related CCR4 among CD8 T cells have a potential ability to exert effector function and migration based on cell markers evaluated. The CCR5 expression would be promoting an enhanced T cell recruitment into liver, a hypothesis that is corroborated by the CCL5/RANTES increase detected in hepatic tissue from dengue fatal cases. The balance between protective and pathogenic immune response mediated by chemokines during dengue fever will be discussed. |
| doi_str_mv | 10.1371/journal.pone.0038527 |
| format | Article |
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Patients from DENV-2 and -3- outbreaks were studied prospectively during the acute or convalescent phases. Expression of chemokine receptor and activation markers on lymphocyte subpopulations were determined by flow cytometry analysis, plasma chemokine ligands concentrations were measured by ELISA and quantification of CCL5/RANTES(+) cells in liver tissues from fatal dengue cases was performed by immunochemistry. In the acute DENV-infection, T-helper/T-cytotoxic type-1 cell (Th1/Tc1)-related CCR5 is significantly higher expressed on both CD4 and CD8 T cells. The Th1-related CXCR3 is up-regulated among CD4 T cells and Tc2-related CCR4 is up-regulated among CD8 T cells. In the convalescent phase, all chemokine receptor or chemokine ligand expression tends to reestablish control healthy levels. Increased CCL2/MCP-1 and CCL4/MIP-1β but decreased CCL5/RANTES levels were observed in DENV-patients during acute infection. Moreover, we showed an increased CD107a expression on CCR5 or CXCR3-expressing T cells and higher expression of CD29, CD44(HIGH) and CD127(LOW) markers on CCR4-expressing CD8 T cells in DENV-patients when compared to controls. Finally, liver from dengue fatal patients showed increased number of cells expressing CCL5/RANTES in three out of four cases compared to three death from a non-dengue patient. In conclusion, both Th1-related CCR5 and CXCR3 among CD4 T cells have a potential ability to exert cytotoxicity function. Moreover, Tc1-related CCR5 and Tc2-related CCR4 among CD8 T cells have a potential ability to exert effector function and migration based on cell markers evaluated. The CCR5 expression would be promoting an enhanced T cell recruitment into liver, a hypothesis that is corroborated by the CCL5/RANTES increase detected in hepatic tissue from dengue fatal cases. The balance between protective and pathogenic immune response mediated by chemokines during dengue fever will be discussed.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0038527</identifier><identifier>PMID: 22815692</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Analysis ; Antigens ; Biology ; Biomarkers - blood ; Biomarkers - metabolism ; Blood circulation ; Blood Platelets - metabolism ; Carbon tetrachloride ; Case-Control Studies ; CCL4 protein ; CCR5 protein ; CD29 antigen ; CD4 antigen ; CD4-Positive T-Lymphocytes - metabolism ; CD8 antigen ; CD8-Positive T-Lymphocytes - metabolism ; Cell activation ; Cell adhesion & migration ; Cell migration ; Chemokine receptors ; Chemokines ; Chemokines - blood ; Chemokines - metabolism ; CXCR3 protein ; Cytokines ; Cytometry ; Cytotoxicity ; Dendritic cells ; Dengue ; Dengue - blood ; Dengue - immunology ; Dengue - metabolism ; Dengue fever ; Dermatitis ; Disease ; Disease control ; Enzyme-linked immunosorbent assay ; Fatalities ; Female ; Fever ; Flow cytometry ; Health aspects ; Hepatocytes ; Humans ; Immune response ; Immune system ; Infections ; Inflammation ; Inflammation - blood ; Inflammation - immunology ; Inflammation - metabolism ; Leukocyte migration ; Ligands ; Liver ; Liver - metabolism ; Lymphocytes ; Lymphocytes T ; Male ; Markers ; Medical research ; Medicine ; Monocyte chemoattractant protein 1 ; Outbreaks ; Pathogenesis ; Patients ; RANTES ; Receptors ; Receptors, Chemokine - metabolism ; Recruitment ; Regulation ; Rodents ; Subpopulations ; T cell receptors ; T cells ; Tissues ; Toxicity ; Up-Regulation ; Vector-borne diseases ; Viral diseases</subject><ispartof>PloS one, 2012-07, Vol.7 (7), p.e38527-e38527</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 de-Oliveira-Pinto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>de-Oliveira-Pinto et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d82aac2aab33967d5620528a1c598dcf6801d8ffd0020443fce6aeee863b68cd3</citedby><cites>FETCH-LOGICAL-c692t-d82aac2aab33967d5620528a1c598dcf6801d8ffd0020443fce6aeee863b68cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398008/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398008/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22815692$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de-Oliveira-Pinto, Luzia Maria</creatorcontrib><creatorcontrib>Marinho, Cíntia Ferreira</creatorcontrib><creatorcontrib>Povoa, Tiago Fajardo</creatorcontrib><creatorcontrib>de Azeredo, Elzinandes Leal</creatorcontrib><creatorcontrib>de Souza, Luiza Assed</creatorcontrib><creatorcontrib>Barbosa, Luiza Damian Ribeiro</creatorcontrib><creatorcontrib>Motta-Castro, Ana Rita C</creatorcontrib><creatorcontrib>Alves, Ada M B</creatorcontrib><creatorcontrib>Ávila, Carlos André Lins</creatorcontrib><creatorcontrib>de Souza, Luiz José</creatorcontrib><creatorcontrib>da Cunha, Rivaldo Venâncio</creatorcontrib><creatorcontrib>Damasco, Paulo Vieira</creatorcontrib><creatorcontrib>Paes, Marciano Viana</creatorcontrib><creatorcontrib>Kubelka, Claire Fernandes</creatorcontrib><title>Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue fever</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Little is known about the role of chemokines/chemokines receptors on T cells in natural DENV infection. Patients from DENV-2 and -3- outbreaks were studied prospectively during the acute or convalescent phases. Expression of chemokine receptor and activation markers on lymphocyte subpopulations were determined by flow cytometry analysis, plasma chemokine ligands concentrations were measured by ELISA and quantification of CCL5/RANTES(+) cells in liver tissues from fatal dengue cases was performed by immunochemistry. In the acute DENV-infection, T-helper/T-cytotoxic type-1 cell (Th1/Tc1)-related CCR5 is significantly higher expressed on both CD4 and CD8 T cells. The Th1-related CXCR3 is up-regulated among CD4 T cells and Tc2-related CCR4 is up-regulated among CD8 T cells. In the convalescent phase, all chemokine receptor or chemokine ligand expression tends to reestablish control healthy levels. Increased CCL2/MCP-1 and CCL4/MIP-1β but decreased CCL5/RANTES levels were observed in DENV-patients during acute infection. Moreover, we showed an increased CD107a expression on CCR5 or CXCR3-expressing T cells and higher expression of CD29, CD44(HIGH) and CD127(LOW) markers on CCR4-expressing CD8 T cells in DENV-patients when compared to controls. Finally, liver from dengue fatal patients showed increased number of cells expressing CCL5/RANTES in three out of four cases compared to three death from a non-dengue patient. In conclusion, both Th1-related CCR5 and CXCR3 among CD4 T cells have a potential ability to exert cytotoxicity function. Moreover, Tc1-related CCR5 and Tc2-related CCR4 among CD8 T cells have a potential ability to exert effector function and migration based on cell markers evaluated. The CCR5 expression would be promoting an enhanced T cell recruitment into liver, a hypothesis that is corroborated by the CCL5/RANTES increase detected in hepatic tissue from dengue fatal cases. The balance between protective and pathogenic immune response mediated by chemokines during dengue fever will be discussed.</description><subject>Adult</subject><subject>Analysis</subject><subject>Antigens</subject><subject>Biology</subject><subject>Biomarkers - blood</subject><subject>Biomarkers - metabolism</subject><subject>Blood circulation</subject><subject>Blood Platelets - metabolism</subject><subject>Carbon tetrachloride</subject><subject>Case-Control Studies</subject><subject>CCL4 protein</subject><subject>CCR5 protein</subject><subject>CD29 antigen</subject><subject>CD4 antigen</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>CD8 antigen</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Cell activation</subject><subject>Cell adhesion & migration</subject><subject>Cell migration</subject><subject>Chemokine receptors</subject><subject>Chemokines</subject><subject>Chemokines - blood</subject><subject>Chemokines - metabolism</subject><subject>CXCR3 protein</subject><subject>Cytokines</subject><subject>Cytometry</subject><subject>Cytotoxicity</subject><subject>Dendritic cells</subject><subject>Dengue</subject><subject>Dengue - blood</subject><subject>Dengue - immunology</subject><subject>Dengue - metabolism</subject><subject>Dengue fever</subject><subject>Dermatitis</subject><subject>Disease</subject><subject>Disease control</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Fatalities</subject><subject>Female</subject><subject>Fever</subject><subject>Flow cytometry</subject><subject>Health aspects</subject><subject>Hepatocytes</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Inflammation - blood</subject><subject>Inflammation - immunology</subject><subject>Inflammation - metabolism</subject><subject>Leukocyte migration</subject><subject>Ligands</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Markers</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Monocyte chemoattractant protein 1</subject><subject>Outbreaks</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>RANTES</subject><subject>Receptors</subject><subject>Receptors, Chemokine - metabolism</subject><subject>Recruitment</subject><subject>Regulation</subject><subject>Rodents</subject><subject>Subpopulations</subject><subject>T cell receptors</subject><subject>T cells</subject><subject>Tissues</subject><subject>Toxicity</subject><subject>Up-Regulation</subject><subject>Vector-borne diseases</subject><subject>Viral diseases</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1trFDEUxwdRbK1-A9GAIPqwazKZS_IilOJloVCo1deQSU5ms2Ym22Sm2A_g9zZ7ad2RPkgICSe_8z85JzlZ9pLgOaE1-bDyY-ilm699D3OMKSvz-lF2TDjNZ1WO6eOD_VH2LMYVxiVlVfU0O8pzRsqK58fZ70toRycH63vkDbK9cbLr5ODDLVJL6PxP2wMKoGCdbBElrHHea3SFFDgXkYzRKysH0GjwaFgCUjaorWTfohsIcYzI2bT5qxdTHKShb0dABtLR8-yJkS7Ci_16kn3__Onq7Ovs_OLL4uz0fKbSZYeZZrmUKs2GUl7Vuky5lTmTRJWcaWUqholmxmiMc1wU1CioJACwijYVU5qeZK93umvno9hXMApCC0ZIwQueiMWO0F6uxDrYToZb4aUVW4MPrZBhsMqB4HVTa0JJLikUDTcc8wZzUylOjOGUJa2P-2hj04FW0A9Buono9KS3S9H6G5GyYxhvBN7tBYK_HiEOorNxU3bZgx_TvXFe07osWZ3QN_-gD2e3p1qZEkiP7VNctREVp0Vd47qmW635A1QaGjqr0nczNtknDu8nDokZ4NfQyjFGsfh2-f_sxY8p-_aAXYJ0wzJ6N25-a5yCxQ5UwccYwNwXmWCx6Za7aohNt4h9tyS3V4cPdO901x70D-rLEjc</recordid><startdate>20120716</startdate><enddate>20120716</enddate><creator>de-Oliveira-Pinto, Luzia Maria</creator><creator>Marinho, Cíntia Ferreira</creator><creator>Povoa, Tiago Fajardo</creator><creator>de Azeredo, Elzinandes Leal</creator><creator>de Souza, Luiza Assed</creator><creator>Barbosa, Luiza Damian Ribeiro</creator><creator>Motta-Castro, Ana Rita C</creator><creator>Alves, Ada M B</creator><creator>Ávila, Carlos André Lins</creator><creator>de Souza, Luiz José</creator><creator>da Cunha, Rivaldo Venâncio</creator><creator>Damasco, Paulo Vieira</creator><creator>Paes, Marciano Viana</creator><creator>Kubelka, Claire Fernandes</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120716</creationdate><title>Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue fever</title><author>de-Oliveira-Pinto, Luzia Maria ; Marinho, Cíntia Ferreira ; Povoa, Tiago Fajardo ; de Azeredo, Elzinandes Leal ; de Souza, Luiza Assed ; Barbosa, Luiza Damian Ribeiro ; Motta-Castro, Ana Rita C ; Alves, Ada M B ; Ávila, Carlos André Lins ; de Souza, Luiz José ; da Cunha, Rivaldo Venâncio ; Damasco, Paulo Vieira ; Paes, Marciano Viana ; Kubelka, Claire Fernandes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-d82aac2aab33967d5620528a1c598dcf6801d8ffd0020443fce6aeee863b68cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Analysis</topic><topic>Antigens</topic><topic>Biology</topic><topic>Biomarkers - blood</topic><topic>Biomarkers - metabolism</topic><topic>Blood circulation</topic><topic>Blood Platelets - metabolism</topic><topic>Carbon tetrachloride</topic><topic>Case-Control Studies</topic><topic>CCL4 protein</topic><topic>CCR5 protein</topic><topic>CD29 antigen</topic><topic>CD4 antigen</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>CD8 antigen</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Cell activation</topic><topic>Cell adhesion & migration</topic><topic>Cell migration</topic><topic>Chemokine receptors</topic><topic>Chemokines</topic><topic>Chemokines - blood</topic><topic>Chemokines - metabolism</topic><topic>CXCR3 protein</topic><topic>Cytokines</topic><topic>Cytometry</topic><topic>Cytotoxicity</topic><topic>Dendritic cells</topic><topic>Dengue</topic><topic>Dengue - blood</topic><topic>Dengue - immunology</topic><topic>Dengue - metabolism</topic><topic>Dengue fever</topic><topic>Dermatitis</topic><topic>Disease</topic><topic>Disease control</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Fatalities</topic><topic>Female</topic><topic>Fever</topic><topic>Flow cytometry</topic><topic>Health aspects</topic><topic>Hepatocytes</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Inflammation - blood</topic><topic>Inflammation - immunology</topic><topic>Inflammation - metabolism</topic><topic>Leukocyte migration</topic><topic>Ligands</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Markers</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Monocyte chemoattractant protein 1</topic><topic>Outbreaks</topic><topic>Pathogenesis</topic><topic>Patients</topic><topic>RANTES</topic><topic>Receptors</topic><topic>Receptors, Chemokine - metabolism</topic><topic>Recruitment</topic><topic>Regulation</topic><topic>Rodents</topic><topic>Subpopulations</topic><topic>T cell receptors</topic><topic>T cells</topic><topic>Tissues</topic><topic>Toxicity</topic><topic>Up-Regulation</topic><topic>Vector-borne diseases</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de-Oliveira-Pinto, Luzia Maria</creatorcontrib><creatorcontrib>Marinho, Cíntia Ferreira</creatorcontrib><creatorcontrib>Povoa, Tiago Fajardo</creatorcontrib><creatorcontrib>de Azeredo, Elzinandes Leal</creatorcontrib><creatorcontrib>de Souza, Luiza Assed</creatorcontrib><creatorcontrib>Barbosa, Luiza Damian Ribeiro</creatorcontrib><creatorcontrib>Motta-Castro, Ana Rita C</creatorcontrib><creatorcontrib>Alves, Ada M B</creatorcontrib><creatorcontrib>Ávila, Carlos André Lins</creatorcontrib><creatorcontrib>de Souza, Luiz José</creatorcontrib><creatorcontrib>da Cunha, Rivaldo Venâncio</creatorcontrib><creatorcontrib>Damasco, Paulo Vieira</creatorcontrib><creatorcontrib>Paes, Marciano Viana</creatorcontrib><creatorcontrib>Kubelka, Claire Fernandes</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de-Oliveira-Pinto, Luzia Maria</au><au>Marinho, Cíntia Ferreira</au><au>Povoa, Tiago Fajardo</au><au>de Azeredo, Elzinandes Leal</au><au>de Souza, Luiza Assed</au><au>Barbosa, Luiza Damian Ribeiro</au><au>Motta-Castro, Ana Rita C</au><au>Alves, Ada M B</au><au>Ávila, Carlos André Lins</au><au>de Souza, Luiz José</au><au>da Cunha, Rivaldo Venâncio</au><au>Damasco, Paulo Vieira</au><au>Paes, Marciano Viana</au><au>Kubelka, Claire Fernandes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue fever</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-07-16</date><risdate>2012</risdate><volume>7</volume><issue>7</issue><spage>e38527</spage><epage>e38527</epage><pages>e38527-e38527</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Little is known about the role of chemokines/chemokines receptors on T cells in natural DENV infection. Patients from DENV-2 and -3- outbreaks were studied prospectively during the acute or convalescent phases. Expression of chemokine receptor and activation markers on lymphocyte subpopulations were determined by flow cytometry analysis, plasma chemokine ligands concentrations were measured by ELISA and quantification of CCL5/RANTES(+) cells in liver tissues from fatal dengue cases was performed by immunochemistry. In the acute DENV-infection, T-helper/T-cytotoxic type-1 cell (Th1/Tc1)-related CCR5 is significantly higher expressed on both CD4 and CD8 T cells. The Th1-related CXCR3 is up-regulated among CD4 T cells and Tc2-related CCR4 is up-regulated among CD8 T cells. In the convalescent phase, all chemokine receptor or chemokine ligand expression tends to reestablish control healthy levels. Increased CCL2/MCP-1 and CCL4/MIP-1β but decreased CCL5/RANTES levels were observed in DENV-patients during acute infection. Moreover, we showed an increased CD107a expression on CCR5 or CXCR3-expressing T cells and higher expression of CD29, CD44(HIGH) and CD127(LOW) markers on CCR4-expressing CD8 T cells in DENV-patients when compared to controls. Finally, liver from dengue fatal patients showed increased number of cells expressing CCL5/RANTES in three out of four cases compared to three death from a non-dengue patient. In conclusion, both Th1-related CCR5 and CXCR3 among CD4 T cells have a potential ability to exert cytotoxicity function. Moreover, Tc1-related CCR5 and Tc2-related CCR4 among CD8 T cells have a potential ability to exert effector function and migration based on cell markers evaluated. The CCR5 expression would be promoting an enhanced T cell recruitment into liver, a hypothesis that is corroborated by the CCL5/RANTES increase detected in hepatic tissue from dengue fatal cases. The balance between protective and pathogenic immune response mediated by chemokines during dengue fever will be discussed.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22815692</pmid><doi>10.1371/journal.pone.0038527</doi><tpages>e38527</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-07, Vol.7 (7), p.e38527-e38527 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1348114949 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Analysis Antigens Biology Biomarkers - blood Biomarkers - metabolism Blood circulation Blood Platelets - metabolism Carbon tetrachloride Case-Control Studies CCL4 protein CCR5 protein CD29 antigen CD4 antigen CD4-Positive T-Lymphocytes - metabolism CD8 antigen CD8-Positive T-Lymphocytes - metabolism Cell activation Cell adhesion & migration Cell migration Chemokine receptors Chemokines Chemokines - blood Chemokines - metabolism CXCR3 protein Cytokines Cytometry Cytotoxicity Dendritic cells Dengue Dengue - blood Dengue - immunology Dengue - metabolism Dengue fever Dermatitis Disease Disease control Enzyme-linked immunosorbent assay Fatalities Female Fever Flow cytometry Health aspects Hepatocytes Humans Immune response Immune system Infections Inflammation Inflammation - blood Inflammation - immunology Inflammation - metabolism Leukocyte migration Ligands Liver Liver - metabolism Lymphocytes Lymphocytes T Male Markers Medical research Medicine Monocyte chemoattractant protein 1 Outbreaks Pathogenesis Patients RANTES Receptors Receptors, Chemokine - metabolism Recruitment Regulation Rodents Subpopulations T cell receptors T cells Tissues Toxicity Up-Regulation Vector-borne diseases Viral diseases |
| title | Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue fever |
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