Potential of olfactory ensheathing cells from different sources for spinal cord repair
Spinal cord injury (SCI) induces a permanent disability in patients. To this day no curative treatment can be proposed to restore lost functions. Therefore, extensive experimental studies have been conducted to induce recovery after SCI. One of the most promising therapies is based on the use of olf...
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| Veröffentlicht in: | PloS one 2013-04, Vol.8 (4), p.e62860-e62860 |
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| creator | Mayeur, Anne Duclos, Célia Honoré, Axel Gauberti, Maxime Drouot, Laurent do Rego, Jean-Claude Bon-Mardion, Nicolas Jean, Laetitia Vérin, Eric Emery, Evelyne Lemarchant, Sighild Vivien, Denis Boyer, Olivier Marie, Jean-Paul Guérout, Nicolas |
| description | Spinal cord injury (SCI) induces a permanent disability in patients. To this day no curative treatment can be proposed to restore lost functions. Therefore, extensive experimental studies have been conducted to induce recovery after SCI. One of the most promising therapies is based on the use of olfactory ensheathing cells (OECs). OECs can be obtained from either the olfactory bulbs (OB-OECs) or from olfactory mucosa (OM-OECs), involving a less invasive approach for autotransplantation. However the vast majority of experimental transplantations have been focusing on OB-OECs although the OM represents a more accessible source of OECs. Importantly, the ability of OM-OECs in comparison to OB-OECs to induce spinal cord recovery in the same lesion paradigm has never been described. We here present data using a multiparametric approach, based on electrophysiological, behavioral, histological and magnetic resonance imaging experiments on the repair potential of OB-OECs and OM-OECs from either primary or purified cultures after a severe model of SCI. Our data demonstrate that transplantation of OECs obtained from OB or OM induces electrophysiological and functional recovery, reduces astrocyte reactivity and glial scar formation and improves axonal regrowth. We also show that the purification step is essential for OM-OECs while not required for OB-OECs. Altogether, our study strongly indicates that transplantation of OECs from OM represents the best benefit/risk ratio according to the safety of access of OM and the results induced by transplantations of OM-OECs. Indeed, purified OM-OECs in addition to induce recovery can integrate and survive up to 60 days into the spinal cord. Therefore, our results provide strong support for these cells as a viable therapy for SCI. |
| doi_str_mv | 10.1371/journal.pone.0062860 |
| format | Article |
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To this day no curative treatment can be proposed to restore lost functions. Therefore, extensive experimental studies have been conducted to induce recovery after SCI. One of the most promising therapies is based on the use of olfactory ensheathing cells (OECs). OECs can be obtained from either the olfactory bulbs (OB-OECs) or from olfactory mucosa (OM-OECs), involving a less invasive approach for autotransplantation. However the vast majority of experimental transplantations have been focusing on OB-OECs although the OM represents a more accessible source of OECs. Importantly, the ability of OM-OECs in comparison to OB-OECs to induce spinal cord recovery in the same lesion paradigm has never been described. We here present data using a multiparametric approach, based on electrophysiological, behavioral, histological and magnetic resonance imaging experiments on the repair potential of OB-OECs and OM-OECs from either primary or purified cultures after a severe model of SCI. Our data demonstrate that transplantation of OECs obtained from OB or OM induces electrophysiological and functional recovery, reduces astrocyte reactivity and glial scar formation and improves axonal regrowth. We also show that the purification step is essential for OM-OECs while not required for OB-OECs. Altogether, our study strongly indicates that transplantation of OECs from OM represents the best benefit/risk ratio according to the safety of access of OM and the results induced by transplantations of OM-OECs. Indeed, purified OM-OECs in addition to induce recovery can integrate and survive up to 60 days into the spinal cord. Therefore, our results provide strong support for these cells as a viable therapy for SCI.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0062860</identifier><identifier>PMID: 23638158</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biology ; Biomedical research ; Care and treatment ; Cell cycle ; Cell Tracking ; Cell Transplantation ; Disease Models, Animal ; Fibroblasts ; Gene Expression ; Genes, Reporter ; Lameness, Animal ; Magnetic resonance ; Magnetic Resonance Imaging ; Male ; Medicine ; Motor Activity ; Mucosa ; Neurons ; Olfactory bulb ; Olfactory Bulb - cytology ; Olfactory cortex ; Olfactory ensheathing cells ; Olfactory epithelium ; Olfactory Mucosa - cytology ; Otolaryngology ; Physiological aspects ; Purification ; Rats ; Recovery ; Recovery of function ; Regrowth ; Repair ; Spinal cord ; Spinal Cord - pathology ; Spinal Cord - physiopathology ; Spinal cord injuries ; Spinal Cord Injuries - diagnosis ; Spinal Cord Injuries - physiopathology ; Spinal Cord Injuries - therapy ; Spinal cord injury ; Spinal Cord Regeneration ; Stem cells ; Surgery ; Synaptic Potentials ; Transgenes ; Transplantation ; Transplants & implants</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e62860-e62860</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Mayeur et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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To this day no curative treatment can be proposed to restore lost functions. Therefore, extensive experimental studies have been conducted to induce recovery after SCI. One of the most promising therapies is based on the use of olfactory ensheathing cells (OECs). OECs can be obtained from either the olfactory bulbs (OB-OECs) or from olfactory mucosa (OM-OECs), involving a less invasive approach for autotransplantation. However the vast majority of experimental transplantations have been focusing on OB-OECs although the OM represents a more accessible source of OECs. Importantly, the ability of OM-OECs in comparison to OB-OECs to induce spinal cord recovery in the same lesion paradigm has never been described. We here present data using a multiparametric approach, based on electrophysiological, behavioral, histological and magnetic resonance imaging experiments on the repair potential of OB-OECs and OM-OECs from either primary or purified cultures after a severe model of SCI. 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pathology</subject><subject>Spinal Cord - physiopathology</subject><subject>Spinal cord injuries</subject><subject>Spinal Cord Injuries - diagnosis</subject><subject>Spinal Cord Injuries - physiopathology</subject><subject>Spinal Cord Injuries - therapy</subject><subject>Spinal cord injury</subject><subject>Spinal Cord Regeneration</subject><subject>Stem cells</subject><subject>Surgery</subject><subject>Synaptic Potentials</subject><subject>Transgenes</subject><subject>Transplantation</subject><subject>Transplants & implants</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkttr2zAUxs3YWNts_8HYDIOyPSTTzbL9Mihll0ChY5e-Cl0dBcVKJXms__2UxC3x6MPwg83R73zn6PNXFK8gWEBcww9rP4Seu8XW93oBAEUNBU-KU9hiNKcI4KdH3yfFWYxrACrcUPq8OEGY4gZWzWlx880n3SfLXelN6Z3hMvlwV-o-rjRPK9t3pdTOxdIEvymVNUaH3FDGPF7qXPahjFubNymlD6oMestteFE8M9xF_XJ8z4pfnz_9vPw6v7r-sry8uJpL2qI054YgxCuCBFW4rhFQuMW1EJhWSBpQEQ0117gxmgNSKS1aJRQwFEEupKoNnhVvDrpb5yMbLYkMYkKrFgNEM7E8EMrzNdsGu-Hhjnlu2b7gQ8d4SFY6zYggAIBGtA1siYFKIFhjCjDipm5aI7LWx3HaIDZayexD4G4iOj3p7Yp1_jfLdpOakCzwbhQI_nbQMbGNjTt7ea_9sN-7IW0L8-6z4u0_6OO3G6mO5wvY3vg8V-5E2QWpG4Aw3lOLR6j8KL2xMufH2FyfNLyfNGQm6T-p40OMbPnj-_-z1zdT9vyIzQFzaRW9G5L1fZyC5ADK4GMM2jyYDAHbxf_eDbaLPxvjn9teH_-gh6b7vOO_noL_7g</recordid><startdate>20130424</startdate><enddate>20130424</enddate><creator>Mayeur, Anne</creator><creator>Duclos, Célia</creator><creator>Honoré, Axel</creator><creator>Gauberti, Maxime</creator><creator>Drouot, Laurent</creator><creator>do Rego, Jean-Claude</creator><creator>Bon-Mardion, Nicolas</creator><creator>Jean, Laetitia</creator><creator>Vérin, Eric</creator><creator>Emery, Evelyne</creator><creator>Lemarchant, Sighild</creator><creator>Vivien, Denis</creator><creator>Boyer, Olivier</creator><creator>Marie, Jean-Paul</creator><creator>Guérout, Nicolas</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130424</creationdate><title>Potential of olfactory ensheathing cells from different sources for spinal cord repair</title><author>Mayeur, Anne ; Duclos, Célia ; Honoré, Axel ; Gauberti, Maxime ; Drouot, Laurent ; do Rego, Jean-Claude ; Bon-Mardion, Nicolas ; Jean, Laetitia ; Vérin, Eric ; Emery, Evelyne ; Lemarchant, Sighild ; Vivien, Denis ; Boyer, Olivier ; Marie, Jean-Paul ; Guérout, Nicolas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-af422a542b6d37720d3937bb3652cf054e1eae38fea045deb9dbd0f621abcd7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biology</topic><topic>Biomedical research</topic><topic>Care and treatment</topic><topic>Cell cycle</topic><topic>Cell Tracking</topic><topic>Cell Transplantation</topic><topic>Disease Models, Animal</topic><topic>Fibroblasts</topic><topic>Gene Expression</topic><topic>Genes, Reporter</topic><topic>Lameness, Animal</topic><topic>Magnetic resonance</topic><topic>Magnetic Resonance Imaging</topic><topic>Male</topic><topic>Medicine</topic><topic>Motor Activity</topic><topic>Mucosa</topic><topic>Neurons</topic><topic>Olfactory bulb</topic><topic>Olfactory Bulb - 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To this day no curative treatment can be proposed to restore lost functions. Therefore, extensive experimental studies have been conducted to induce recovery after SCI. One of the most promising therapies is based on the use of olfactory ensheathing cells (OECs). OECs can be obtained from either the olfactory bulbs (OB-OECs) or from olfactory mucosa (OM-OECs), involving a less invasive approach for autotransplantation. However the vast majority of experimental transplantations have been focusing on OB-OECs although the OM represents a more accessible source of OECs. Importantly, the ability of OM-OECs in comparison to OB-OECs to induce spinal cord recovery in the same lesion paradigm has never been described. We here present data using a multiparametric approach, based on electrophysiological, behavioral, histological and magnetic resonance imaging experiments on the repair potential of OB-OECs and OM-OECs from either primary or purified cultures after a severe model of SCI. Our data demonstrate that transplantation of OECs obtained from OB or OM induces electrophysiological and functional recovery, reduces astrocyte reactivity and glial scar formation and improves axonal regrowth. We also show that the purification step is essential for OM-OECs while not required for OB-OECs. Altogether, our study strongly indicates that transplantation of OECs from OM represents the best benefit/risk ratio according to the safety of access of OM and the results induced by transplantations of OM-OECs. Indeed, purified OM-OECs in addition to induce recovery can integrate and survive up to 60 days into the spinal cord. Therefore, our results provide strong support for these cells as a viable therapy for SCI.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23638158</pmid><doi>10.1371/journal.pone.0062860</doi><tpages>e62860</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_1346593026 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animals Biology Biomedical research Care and treatment Cell cycle Cell Tracking Cell Transplantation Disease Models, Animal Fibroblasts Gene Expression Genes, Reporter Lameness, Animal Magnetic resonance Magnetic Resonance Imaging Male Medicine Motor Activity Mucosa Neurons Olfactory bulb Olfactory Bulb - cytology Olfactory cortex Olfactory ensheathing cells Olfactory epithelium Olfactory Mucosa - cytology Otolaryngology Physiological aspects Purification Rats Recovery Recovery of function Regrowth Repair Spinal cord Spinal Cord - pathology Spinal Cord - physiopathology Spinal cord injuries Spinal Cord Injuries - diagnosis Spinal Cord Injuries - physiopathology Spinal Cord Injuries - therapy Spinal cord injury Spinal Cord Regeneration Stem cells Surgery Synaptic Potentials Transgenes Transplantation Transplants & implants |
| title | Potential of olfactory ensheathing cells from different sources for spinal cord repair |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T19%3A35%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Potential%20of%20olfactory%20ensheathing%20cells%20from%20different%20sources%20for%20spinal%20cord%20repair&rft.jtitle=PloS%20one&rft.au=Mayeur,%20Anne&rft.date=2013-04-24&rft.volume=8&rft.issue=4&rft.spage=e62860&rft.epage=e62860&rft.pages=e62860-e62860&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0062860&rft_dat=%3Cgale_plos_%3EA478023326%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1346593026&rft_id=info:pmid/23638158&rft_galeid=A478023326&rft_doaj_id=oai_doaj_org_article_4b40008b98194f1db21736032af789fb&rfr_iscdi=true |