Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica
Ubiquitin, an 8.5 kDa protein associated with the proteasome degradation pathway has been recently identified as differentially expressed in segment of cord caudal to site of injury in developing spinal cord. Here we describe ubiquitin expression and cellular distribution in spinal cord up to postna...
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| Veröffentlicht in: | PloS one 2013-04, Vol.8 (4), p.e62120-e62120 |
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| creator | Noor, Natassya M Møllgård, Kjeld Wheaton, Benjamin J Steer, David L Truettner, Jessie S Dziegielewska, Katarzyna M Dietrich, W Dalton Smith, A Ian Saunders, Norman R |
| description | Ubiquitin, an 8.5 kDa protein associated with the proteasome degradation pathway has been recently identified as differentially expressed in segment of cord caudal to site of injury in developing spinal cord. Here we describe ubiquitin expression and cellular distribution in spinal cord up to postnatal day P35 in control opossums (Monodelphis domestica) and in response to complete spinal transection (T10) at P7, when axonal growth through site of injury occurs, and P28 when this is no longer possible. Cords were collected 1 or 7 days after injury, with age-matched controls and segments rostral to lesion were studied. Following spinal injury ubiquitin levels (western blotting) appeared reduced compared to controls especially one day after injury at P28. In contrast, after injury mRNA expression (qRT-PCR) was slightly increased at P7 but decreased at P28. Changes in isoelectric point of separated ubiquitin indicated possible post-translational modifications. Cellular distribution demonstrated a developmental shift between earliest (P8) and latest (P35) ages examined, from a predominantly cytoplasmic immunoreactivity to a nuclear expression; staining level and shift to nuclear staining was more pronounced following injury, except 7 days after transection at P28. After injury at P7 immunostaining increased in neurons and additionally in oligodendrocytes at P28. Mass spectrometry showed two ubiquitin bands; the heavier was identified as a fusion product, likely to be an ubiquitin precursor. Apparent changes in ubiquitin expression and cellular distribution in development and response to spinal injury suggest an intricate regulatory system that modulates these responses which, when better understood, may lead to potential therapeutic targets. |
| doi_str_mv | 10.1371/journal.pone.0062120 |
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Here we describe ubiquitin expression and cellular distribution in spinal cord up to postnatal day P35 in control opossums (Monodelphis domestica) and in response to complete spinal transection (T10) at P7, when axonal growth through site of injury occurs, and P28 when this is no longer possible. Cords were collected 1 or 7 days after injury, with age-matched controls and segments rostral to lesion were studied. Following spinal injury ubiquitin levels (western blotting) appeared reduced compared to controls especially one day after injury at P28. In contrast, after injury mRNA expression (qRT-PCR) was slightly increased at P7 but decreased at P28. Changes in isoelectric point of separated ubiquitin indicated possible post-translational modifications. Cellular distribution demonstrated a developmental shift between earliest (P8) and latest (P35) ages examined, from a predominantly cytoplasmic immunoreactivity to a nuclear expression; staining level and shift to nuclear staining was more pronounced following injury, except 7 days after transection at P28. After injury at P7 immunostaining increased in neurons and additionally in oligodendrocytes at P28. Mass spectrometry showed two ubiquitin bands; the heavier was identified as a fusion product, likely to be an ubiquitin precursor. Apparent changes in ubiquitin expression and cellular distribution in development and response to spinal injury suggest an intricate regulatory system that modulates these responses which, when better understood, may lead to potential therapeutic targets.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0062120</identifier><identifier>PMID: 23626776</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Age ; Animals ; Animals, Newborn ; Biochemistry ; Biology ; Ethics ; Gene Expression ; Genomes ; Glycoproteins ; Immunohistochemistry ; Immunoreactivity ; Injury prevention ; Mass spectrometry ; Mass spectroscopy ; Molecular biology ; Monodelphis ; Monodelphis - metabolism ; Monodelphis domestica ; Oligodendrocytes ; Paralysis ; Pharmacology ; Post-translation ; Proteasomes ; Protein expression ; Protein Transport ; Proteins ; Proteome ; Proteomics ; RNA ; Rodents ; Spinal cord injuries ; Spinal Cord Injuries - genetics ; Spinal Cord Injuries - metabolism ; Staining ; Studies ; Trauma ; Ubiquitin ; Ubiquitin - genetics ; Ubiquitin - metabolism ; Western blotting</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e62120-e62120</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Noor et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Apparent changes in ubiquitin expression and cellular distribution in development and response to spinal injury suggest an intricate regulatory system that modulates these responses which, when better understood, may lead to potential therapeutic targets.</description><subject>Age</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biochemistry</subject><subject>Biology</subject><subject>Ethics</subject><subject>Gene Expression</subject><subject>Genomes</subject><subject>Glycoproteins</subject><subject>Immunohistochemistry</subject><subject>Immunoreactivity</subject><subject>Injury prevention</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Molecular biology</subject><subject>Monodelphis</subject><subject>Monodelphis - metabolism</subject><subject>Monodelphis domestica</subject><subject>Oligodendrocytes</subject><subject>Paralysis</subject><subject>Pharmacology</subject><subject>Post-translation</subject><subject>Proteasomes</subject><subject>Protein expression</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>Proteome</subject><subject>Proteomics</subject><subject>RNA</subject><subject>Rodents</subject><subject>Spinal cord injuries</subject><subject>Spinal Cord Injuries - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Noor, Natassya M</au><au>Møllgård, Kjeld</au><au>Wheaton, Benjamin J</au><au>Steer, David L</au><au>Truettner, Jessie S</au><au>Dziegielewska, Katarzyna M</au><au>Dietrich, W Dalton</au><au>Smith, A Ian</au><au>Saunders, Norman R</au><au>Di Giovanni, Simone</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-04-23</date><risdate>2013</risdate><volume>8</volume><issue>4</issue><spage>e62120</spage><epage>e62120</epage><pages>e62120-e62120</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Ubiquitin, an 8.5 kDa protein associated with the proteasome degradation pathway has been recently identified as differentially expressed in segment of cord caudal to site of injury in developing spinal cord. Here we describe ubiquitin expression and cellular distribution in spinal cord up to postnatal day P35 in control opossums (Monodelphis domestica) and in response to complete spinal transection (T10) at P7, when axonal growth through site of injury occurs, and P28 when this is no longer possible. Cords were collected 1 or 7 days after injury, with age-matched controls and segments rostral to lesion were studied. Following spinal injury ubiquitin levels (western blotting) appeared reduced compared to controls especially one day after injury at P28. In contrast, after injury mRNA expression (qRT-PCR) was slightly increased at P7 but decreased at P28. Changes in isoelectric point of separated ubiquitin indicated possible post-translational modifications. Cellular distribution demonstrated a developmental shift between earliest (P8) and latest (P35) ages examined, from a predominantly cytoplasmic immunoreactivity to a nuclear expression; staining level and shift to nuclear staining was more pronounced following injury, except 7 days after transection at P28. After injury at P7 immunostaining increased in neurons and additionally in oligodendrocytes at P28. Mass spectrometry showed two ubiquitin bands; the heavier was identified as a fusion product, likely to be an ubiquitin precursor. Apparent changes in ubiquitin expression and cellular distribution in development and response to spinal injury suggest an intricate regulatory system that modulates these responses which, when better understood, may lead to potential therapeutic targets.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23626776</pmid><doi>10.1371/journal.pone.0062120</doi><tpages>e62120</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2013-04, Vol.8 (4), p.e62120-e62120 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Age Animals Animals, Newborn Biochemistry Biology Ethics Gene Expression Genomes Glycoproteins Immunohistochemistry Immunoreactivity Injury prevention Mass spectrometry Mass spectroscopy Molecular biology Monodelphis Monodelphis - metabolism Monodelphis domestica Oligodendrocytes Paralysis Pharmacology Post-translation Proteasomes Protein expression Protein Transport Proteins Proteome Proteomics RNA Rodents Spinal cord injuries Spinal Cord Injuries - genetics Spinal Cord Injuries - metabolism Staining Studies Trauma Ubiquitin Ubiquitin - genetics Ubiquitin - metabolism Western blotting |
| title | Expression and cellular distribution of ubiquitin in response to injury in the developing spinal cord of Monodelphis domestica |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T19%3A39%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20and%20cellular%20distribution%20of%20ubiquitin%20in%20response%20to%20injury%20in%20the%20developing%20spinal%20cord%20of%20Monodelphis%20domestica&rft.jtitle=PloS%20one&rft.au=Noor,%20Natassya%20M&rft.date=2013-04-23&rft.volume=8&rft.issue=4&rft.spage=e62120&rft.epage=e62120&rft.pages=e62120-e62120&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0062120&rft_dat=%3Cgale_plos_%3EA478152406%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1346589856&rft_id=info:pmid/23626776&rft_galeid=A478152406&rft_doaj_id=oai_doaj_org_article_7108b20080604f5d873452fc9f76638f&rfr_iscdi=true |