Phenotypic and genotypic characteristics of Neisseria meningitidis disease-causing strains in Argentina, 2010
Phenotypic and genotypic characterization of 133 isolates of Neisseria meningitidis obtained from meningococcal disease cases in Argentina during 2010 were performed by the National Reference Laboratory as part of a project coordinated by the PAHO within the SIREVA II network. Serogroup, serotype, s...
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| description | Phenotypic and genotypic characterization of 133 isolates of Neisseria meningitidis obtained from meningococcal disease cases in Argentina during 2010 were performed by the National Reference Laboratory as part of a project coordinated by the PAHO within the SIREVA II network. Serogroup, serotype, serosubtype and MLST characterization were performed. Minimum Inhibitory Concentration to penicillin, ampicillin, ceftriaxone, rifampin, chloramphenicol, tetracycline and ciprofloxacin were determined and interpreted according to CLSI guidelines. Almost 49% of isolates were W135, and two serotype:serosubtype combinations, W135:2a:P1.5,2:ST-11 and W135:2a:P1.2:ST-11 accounted for 78% of all W135 isolates. Serogroup B accounted for 42.1% of isolates, and was both phenotypically and genotypically diverse. Serogroup C isolates represented 5.3% of the dataset, and one isolate belonging to the ST-198 complex was non-groupable. Isolates belonged mainly to the ST-11 complex (48%) and to a lesser extent to the ST-865 (18%), ST-32 (9,8%) and the ST-35 complexes (9%). Intermediate resistance to penicillin and ampicillin was detected in 35.4% and 33.1% of isolates respectively. Two W135:2a:P1.5,2:ST-11:ST-11 isolates presented resistance to ciprofloxacin associated with a mutation in the QRDR of gyrA gene Thr91-Ile. These data show serogroup W135 was the first cause of disease in Argentina in 2010, and was strongly associated with the W135:2a:P1.5,2:ST-11 epidemic clone. Serogroup B was the second cause of disease and isolates belonging to this serogroup were phenotypically and genotypically diverse. The presence of isolates with intermediate resistance to penicillin and the presence of fluorquinolone-resistant isolates highlight the necessity and importance of maintaining and strengthening National Surveillance Programs. |
| doi_str_mv | 10.1371/journal.pone.0058065 |
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Serogroup, serotype, serosubtype and MLST characterization were performed. Minimum Inhibitory Concentration to penicillin, ampicillin, ceftriaxone, rifampin, chloramphenicol, tetracycline and ciprofloxacin were determined and interpreted according to CLSI guidelines. Almost 49% of isolates were W135, and two serotype:serosubtype combinations, W135:2a:P1.5,2:ST-11 and W135:2a:P1.2:ST-11 accounted for 78% of all W135 isolates. Serogroup B accounted for 42.1% of isolates, and was both phenotypically and genotypically diverse. Serogroup C isolates represented 5.3% of the dataset, and one isolate belonging to the ST-198 complex was non-groupable. Isolates belonged mainly to the ST-11 complex (48%) and to a lesser extent to the ST-865 (18%), ST-32 (9,8%) and the ST-35 complexes (9%). Intermediate resistance to penicillin and ampicillin was detected in 35.4% and 33.1% of isolates respectively. Two W135:2a:P1.5,2:ST-11:ST-11 isolates presented resistance to ciprofloxacin associated with a mutation in the QRDR of gyrA gene Thr91-Ile. These data show serogroup W135 was the first cause of disease in Argentina in 2010, and was strongly associated with the W135:2a:P1.5,2:ST-11 epidemic clone. Serogroup B was the second cause of disease and isolates belonging to this serogroup were phenotypically and genotypically diverse. The presence of isolates with intermediate resistance to penicillin and the presence of fluorquinolone-resistant isolates highlight the necessity and importance of maintaining and strengthening National Surveillance Programs.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0058065</identifier><identifier>PMID: 23483970</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Ampicillin ; Anti-Infective Agents - pharmacology ; Antibiotics ; Antimicrobial agents ; Argentina ; Bacteriology ; Causes of ; Ceftriaxone ; Chloramphenicol ; Ciprofloxacin ; Ciprofloxacin - pharmacology ; DNA topoisomerase ; Drug resistance ; Drug Resistance, Bacterial - drug effects ; Drug Resistance, Bacterial - genetics ; Epidemics ; Genetic aspects ; Genotype ; Humans ; Infectious diseases ; Laboratories ; Medicine ; Meningitis ; Meningitis, Meningococcal - microbiology ; Meningococcal disease ; Microbial Sensitivity Tests ; Minimum inhibitory concentration ; Mutation ; Neisseria ; Neisseria meningitidis ; Neisseria meningitidis - classification ; Neisseria meningitidis - drug effects ; Neisseria meningitidis - genetics ; Neisseria meningitidis - isolation & purification ; Penicillin ; Phenotype ; Rifampin ; Surveillance ; Working groups</subject><ispartof>PloS one, 2013-03, Vol.8 (3), p.e58065-e58065</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Sorhouet-Pereira et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Sorhouet-Pereira et al 2013 Sorhouet-Pereira et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-61746c1508619ef4e9493a4062d054a2797b1d8bd668fa18b9711056079cfa7c3</citedby><cites>FETCH-LOGICAL-c692t-61746c1508619ef4e9493a4062d054a2797b1d8bd668fa18b9711056079cfa7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587574/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587574/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23483970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sorhouet-Pereira, Cecilia</creatorcontrib><creatorcontrib>Efron, Adriana</creatorcontrib><creatorcontrib>Gagetti, Paula</creatorcontrib><creatorcontrib>Faccone, Diego</creatorcontrib><creatorcontrib>Regueira, Mabel</creatorcontrib><creatorcontrib>Corso, Alejandra</creatorcontrib><creatorcontrib>Gabastou, Jean-Marc</creatorcontrib><creatorcontrib>Ibarz-Pavón, Ana Belén</creatorcontrib><creatorcontrib>Argentinean SIREVA II Working Group</creatorcontrib><title>Phenotypic and genotypic characteristics of Neisseria meningitidis disease-causing strains in Argentina, 2010</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Phenotypic and genotypic characterization of 133 isolates of Neisseria meningitidis obtained from meningococcal disease cases in Argentina during 2010 were performed by the National Reference Laboratory as part of a project coordinated by the PAHO within the SIREVA II network. 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Two W135:2a:P1.5,2:ST-11:ST-11 isolates presented resistance to ciprofloxacin associated with a mutation in the QRDR of gyrA gene Thr91-Ile. These data show serogroup W135 was the first cause of disease in Argentina in 2010, and was strongly associated with the W135:2a:P1.5,2:ST-11 epidemic clone. Serogroup B was the second cause of disease and isolates belonging to this serogroup were phenotypically and genotypically diverse. The presence of isolates with intermediate resistance to penicillin and the presence of fluorquinolone-resistant isolates highlight the necessity and importance of maintaining and strengthening National Surveillance Programs.</description><subject>Ampicillin</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Argentina</subject><subject>Bacteriology</subject><subject>Causes of</subject><subject>Ceftriaxone</subject><subject>Chloramphenicol</subject><subject>Ciprofloxacin</subject><subject>Ciprofloxacin - pharmacology</subject><subject>DNA topoisomerase</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial - drug effects</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>Epidemics</subject><subject>Genetic aspects</subject><subject>Genotype</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Laboratories</subject><subject>Medicine</subject><subject>Meningitis</subject><subject>Meningitis, Meningococcal - microbiology</subject><subject>Meningococcal disease</subject><subject>Microbial Sensitivity Tests</subject><subject>Minimum inhibitory concentration</subject><subject>Mutation</subject><subject>Neisseria</subject><subject>Neisseria meningitidis</subject><subject>Neisseria meningitidis - classification</subject><subject>Neisseria meningitidis - drug effects</subject><subject>Neisseria meningitidis - genetics</subject><subject>Neisseria meningitidis - isolation & purification</subject><subject>Penicillin</subject><subject>Phenotype</subject><subject>Rifampin</subject><subject>Surveillance</subject><subject>Working groups</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk2uL1DAUhoso7rr6D0QLgig4Y9KkuXwRhsXLwOKKt6_hNE07WdpkNmnF_fdmnO4wlf0gJbQ9ec6bnDc5WfYUoyUmHL-98mNw0C233pklQqVArLyXnWJJigUrELl_9H2SPYrxKkFEMPYwOykIFURydJr1XzbG-eFma3UOrs7bw5_eQAA9mGDjYHXMfZN_NjbGFIC8N8661g62tjFPw0A0Cw1jTNE8DgGsi7l1-SokwcE6eJMXCKPH2YMGumieTO-z7MeH99_PPy0uLj-uz1cXC81kMSwY5pRpXCLBsDQNNZJKAhSxokYlhYJLXuFaVDVjogEsKskxRiVDXOoGuCZn2fO97rbzUU1ORYUJZaXgTJSJWO-J2sOV2gbbQ7hRHqz6G_ChVRBS3Z1RoCsKZTKs4YYS3UhRVaysJaqlbCpJkta7abWx6k2tU8UBupnofMbZjWr9L0XSZkpOk8CrSSD469HEQfU2atN14Iwfd_tOhiCOix364h_07uomqoVUgHWNT-vqnahaUS5oITjCiVreQaWnNr3V6Vo1NsVnCa9nCYkZzO-hTece1frb1_9nL3_O2ZdH7MZAN2yi78bBehfnIN2DOvgYg2kOJmOkdl1x64badYWauiKlPTs-oEPSbRuQP-PGBzo</recordid><startdate>20130304</startdate><enddate>20130304</enddate><creator>Sorhouet-Pereira, Cecilia</creator><creator>Efron, Adriana</creator><creator>Gagetti, Paula</creator><creator>Faccone, Diego</creator><creator>Regueira, Mabel</creator><creator>Corso, Alejandra</creator><creator>Gabastou, Jean-Marc</creator><creator>Ibarz-Pavón, Ana Belén</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130304</creationdate><title>Phenotypic and genotypic characteristics of Neisseria meningitidis disease-causing strains in Argentina, 2010</title><author>Sorhouet-Pereira, Cecilia ; Efron, Adriana ; Gagetti, Paula ; Faccone, Diego ; Regueira, Mabel ; Corso, Alejandra ; Gabastou, Jean-Marc ; Ibarz-Pavón, Ana Belén</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-61746c1508619ef4e9493a4062d054a2797b1d8bd668fa18b9711056079cfa7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Ampicillin</topic><topic>Anti-Infective Agents - 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Serogroup, serotype, serosubtype and MLST characterization were performed. Minimum Inhibitory Concentration to penicillin, ampicillin, ceftriaxone, rifampin, chloramphenicol, tetracycline and ciprofloxacin were determined and interpreted according to CLSI guidelines. Almost 49% of isolates were W135, and two serotype:serosubtype combinations, W135:2a:P1.5,2:ST-11 and W135:2a:P1.2:ST-11 accounted for 78% of all W135 isolates. Serogroup B accounted for 42.1% of isolates, and was both phenotypically and genotypically diverse. Serogroup C isolates represented 5.3% of the dataset, and one isolate belonging to the ST-198 complex was non-groupable. Isolates belonged mainly to the ST-11 complex (48%) and to a lesser extent to the ST-865 (18%), ST-32 (9,8%) and the ST-35 complexes (9%). Intermediate resistance to penicillin and ampicillin was detected in 35.4% and 33.1% of isolates respectively. Two W135:2a:P1.5,2:ST-11:ST-11 isolates presented resistance to ciprofloxacin associated with a mutation in the QRDR of gyrA gene Thr91-Ile. These data show serogroup W135 was the first cause of disease in Argentina in 2010, and was strongly associated with the W135:2a:P1.5,2:ST-11 epidemic clone. Serogroup B was the second cause of disease and isolates belonging to this serogroup were phenotypically and genotypically diverse. The presence of isolates with intermediate resistance to penicillin and the presence of fluorquinolone-resistant isolates highlight the necessity and importance of maintaining and strengthening National Surveillance Programs.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23483970</pmid><doi>10.1371/journal.pone.0058065</doi><tpages>e58065</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_1346587685 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Ampicillin Anti-Infective Agents - pharmacology Antibiotics Antimicrobial agents Argentina Bacteriology Causes of Ceftriaxone Chloramphenicol Ciprofloxacin Ciprofloxacin - pharmacology DNA topoisomerase Drug resistance Drug Resistance, Bacterial - drug effects Drug Resistance, Bacterial - genetics Epidemics Genetic aspects Genotype Humans Infectious diseases Laboratories Medicine Meningitis Meningitis, Meningococcal - microbiology Meningococcal disease Microbial Sensitivity Tests Minimum inhibitory concentration Mutation Neisseria Neisseria meningitidis Neisseria meningitidis - classification Neisseria meningitidis - drug effects Neisseria meningitidis - genetics Neisseria meningitidis - isolation & purification Penicillin Phenotype Rifampin Surveillance Working groups |
| title | Phenotypic and genotypic characteristics of Neisseria meningitidis disease-causing strains in Argentina, 2010 |
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