Differential expression of CRH, UCN, CRHR1 and CRHR2 in eutopic and ectopic endometrium of women with endometriosis
Endometriosis is considered as a benign aseptic inflammatory disease, characterised by the presence of ectopic endometrium-like tissue. Its symptoms (mostly pain and infertility) are reported as constant stressors. Corticotropin releasing hormone (CRH) and urocortin (UCN) are neuropeptides, strongly...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-04, Vol.8 (4), p.e62313-e62313 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e62313 |
|---|---|
| container_issue | 4 |
| container_start_page | e62313 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Vergetaki, Aikaterini Jeschke, Udo Vrekoussis, Thomas Taliouri, Eirini Sabatini, Luca Papakonstanti, Evangelia A Makrigiannakis, Antonis |
| description | Endometriosis is considered as a benign aseptic inflammatory disease, characterised by the presence of ectopic endometrium-like tissue. Its symptoms (mostly pain and infertility) are reported as constant stressors. Corticotropin releasing hormone (CRH) and urocortin (UCN) are neuropeptides, strongly related to stress and inflammation. The effects of CRH and UCN are mediated through CRHR1 and CRHR2 receptors which are implicated in several reproductive functions acting as inflammatory components. However, the involvement of these molecules to endometriosis remains unknown. The aim of this study was to examine the expression of CRHR1 and CRHR2 in endometriotic sites and to compare the expression of CRHR1 and CRHR2 in eutopic endometrium of endometriotic women to that of healthy women. We further compared the expression of CRH, UCN, CRHR1 and CRHR2 in ectopic endometrium to that in eutopic endometrium of women with endometriosis. Endometrial biopsy specimens were taken from healthy women (10 patients) and endometrial and endometriotic biopsy specimens were taken from women with endometriosis (16 patients). Τhe expression of CRH, UCN, CRHR1, and CRHR2 was tested via RT-PCR, immunohistochemistry and Western blotting. This study shows for the first time that CRH and UCN receptor subtypes CRHR1β and CRHR2α are expressed in endometriotic sites and that they are more strongly expressed (p |
| doi_str_mv | 10.1371/journal.pone.0062313 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1346157368</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478023318</galeid><doaj_id>oai_doaj_org_article_ab28bfabaea64504a5fe952891914b35</doaj_id><sourcerecordid>A478023318</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-ef50c03e6c483135f910aaca89464d72a7cf21772d69923d83d35b82827a06aa3</originalsourceid><addsrcrecordid>eNqNk21v0zAQxyMEYmPwDRBEQkIgrcUPieO8QZrKwypNTCqMt9bFsVtXSZzZCRvfHqfNRoP2AvmFL-ff_Z2780XRS4zmmGb4w9b2roFq3tpGzRFihGL6KDrGOSUzRhB9fGAfRc-83yKUUs7Y0-iIUEY5oulx5D8ZrZVTTWegitVt65T3xjax1fFidX4aXy2-nQ7WCsfQlDuLxKaJVd_Z1sidU8m9rZrS1qpzpq-H-Jvw0cQ3ptv8PbHe-OfREw2VVy_G_SS6-vL5x-J8dnH5dbk4u5hJlpNupnSKJKKKyYSH3FKdYwQggecJS8qMQCY1wVlGSpbnhJacljQtOOEkA8QA6En0eq_bVtaLsV5eYJownGaU8UAs90RpYStaZ2pwv4UFI3YO69YCXGdkpQQUhBcaClDAkhQlkGqVp4TnOMdJQdOg9XG8rS9qVcpQUgfVRHR60piNWNtfIvQiycgg8G4UcPa6V74TtfFSVRU0yva7_-ZJnoe-BfTNP-jD2Y3UGkICptE23CsHUXGWZBwRSvFAzR-gwipVbWR4XNoE_yTg_SQgMJ267dbQey-W31f_z17-nLJvD9iNgqrbeFv1XXiOfgome1A6671T-r7IGIlhNu6qIYbZEONshLBXhw26D7obBvoHSoAHbA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1346157368</pqid></control><display><type>article</type><title>Differential expression of CRH, UCN, CRHR1 and CRHR2 in eutopic and ectopic endometrium of women with endometriosis</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Vergetaki, Aikaterini ; Jeschke, Udo ; Vrekoussis, Thomas ; Taliouri, Eirini ; Sabatini, Luca ; Papakonstanti, Evangelia A ; Makrigiannakis, Antonis</creator><contributor>Critchley, Hilary OD</contributor><creatorcontrib>Vergetaki, Aikaterini ; Jeschke, Udo ; Vrekoussis, Thomas ; Taliouri, Eirini ; Sabatini, Luca ; Papakonstanti, Evangelia A ; Makrigiannakis, Antonis ; Critchley, Hilary OD</creatorcontrib><description>Endometriosis is considered as a benign aseptic inflammatory disease, characterised by the presence of ectopic endometrium-like tissue. Its symptoms (mostly pain and infertility) are reported as constant stressors. Corticotropin releasing hormone (CRH) and urocortin (UCN) are neuropeptides, strongly related to stress and inflammation. The effects of CRH and UCN are mediated through CRHR1 and CRHR2 receptors which are implicated in several reproductive functions acting as inflammatory components. However, the involvement of these molecules to endometriosis remains unknown. The aim of this study was to examine the expression of CRHR1 and CRHR2 in endometriotic sites and to compare the expression of CRHR1 and CRHR2 in eutopic endometrium of endometriotic women to that of healthy women. We further compared the expression of CRH, UCN, CRHR1 and CRHR2 in ectopic endometrium to that in eutopic endometrium of women with endometriosis. Endometrial biopsy specimens were taken from healthy women (10 patients) and endometrial and endometriotic biopsy specimens were taken from women with endometriosis (16 patients). Τhe expression of CRH, UCN, CRHR1, and CRHR2 was tested via RT-PCR, immunohistochemistry and Western blotting. This study shows for the first time that CRH and UCN receptor subtypes CRHR1β and CRHR2α are expressed in endometriotic sites and that they are more strongly expressed (p<0.01) in eutopic endometrium of women with endometriosis compared to healthy women endometrium at the mRNA and protein level. CRH, UCN, CRHR1 and CRHR2 mRNA were also more highly expressed in ectopic rather than eutopic endometrium (CRH, UCN, CRHR2α: p<0.01, CRHR1β: p<0.05) and protein (CRH and UCN: p<0.05, CRHR1 and CRHR2: p<0.01) in women with endometriosis. These data indicate that CRH and UCN might play an immunoregulatory role in endometriotic sites by affecting reproductive functions such as decidualization and implantation of women with endometriosis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0062313</identifier><identifier>PMID: 23638035</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Biology ; Biopsy ; Case-Control Studies ; Corticotropin-releasing hormone ; Corticotropin-Releasing Hormone - genetics ; Corticotropin-Releasing Hormone - metabolism ; Cytokines ; Endocrinology ; Endometriosis ; Endometriosis - genetics ; Endometriosis - metabolism ; Endometriosis - pathology ; Endometrium ; Endometrium - metabolism ; Female ; Gene expression ; Gene Expression Regulation ; Genetic aspects ; Gynecology ; Humans ; Immunohistochemistry ; Immunoregulation ; Implantation ; Infertility ; Medical schools ; Medicine ; Menstruation ; mRNA ; Neurohormones ; Neuropeptides ; Obstetrics ; Pain ; Pathogenesis ; Patients ; Physiological aspects ; Polymerase chain reaction ; Receptors ; Receptors, Corticotropin-Releasing Hormone - genetics ; Receptors, Corticotropin-Releasing Hormone - metabolism ; Risk factors ; Rodents ; Urocortin ; Urocortins - genetics ; Urocortins - metabolism ; Western blotting ; Womens health</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e62313-e62313</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Vergetaki et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Vergetaki et al 2013 Vergetaki et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-ef50c03e6c483135f910aaca89464d72a7cf21772d69923d83d35b82827a06aa3</citedby><cites>FETCH-LOGICAL-c692t-ef50c03e6c483135f910aaca89464d72a7cf21772d69923d83d35b82827a06aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634725/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634725/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23638035$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Critchley, Hilary OD</contributor><creatorcontrib>Vergetaki, Aikaterini</creatorcontrib><creatorcontrib>Jeschke, Udo</creatorcontrib><creatorcontrib>Vrekoussis, Thomas</creatorcontrib><creatorcontrib>Taliouri, Eirini</creatorcontrib><creatorcontrib>Sabatini, Luca</creatorcontrib><creatorcontrib>Papakonstanti, Evangelia A</creatorcontrib><creatorcontrib>Makrigiannakis, Antonis</creatorcontrib><title>Differential expression of CRH, UCN, CRHR1 and CRHR2 in eutopic and ectopic endometrium of women with endometriosis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Endometriosis is considered as a benign aseptic inflammatory disease, characterised by the presence of ectopic endometrium-like tissue. Its symptoms (mostly pain and infertility) are reported as constant stressors. Corticotropin releasing hormone (CRH) and urocortin (UCN) are neuropeptides, strongly related to stress and inflammation. The effects of CRH and UCN are mediated through CRHR1 and CRHR2 receptors which are implicated in several reproductive functions acting as inflammatory components. However, the involvement of these molecules to endometriosis remains unknown. The aim of this study was to examine the expression of CRHR1 and CRHR2 in endometriotic sites and to compare the expression of CRHR1 and CRHR2 in eutopic endometrium of endometriotic women to that of healthy women. We further compared the expression of CRH, UCN, CRHR1 and CRHR2 in ectopic endometrium to that in eutopic endometrium of women with endometriosis. Endometrial biopsy specimens were taken from healthy women (10 patients) and endometrial and endometriotic biopsy specimens were taken from women with endometriosis (16 patients). Τhe expression of CRH, UCN, CRHR1, and CRHR2 was tested via RT-PCR, immunohistochemistry and Western blotting. This study shows for the first time that CRH and UCN receptor subtypes CRHR1β and CRHR2α are expressed in endometriotic sites and that they are more strongly expressed (p<0.01) in eutopic endometrium of women with endometriosis compared to healthy women endometrium at the mRNA and protein level. CRH, UCN, CRHR1 and CRHR2 mRNA were also more highly expressed in ectopic rather than eutopic endometrium (CRH, UCN, CRHR2α: p<0.01, CRHR1β: p<0.05) and protein (CRH and UCN: p<0.05, CRHR1 and CRHR2: p<0.01) in women with endometriosis. These data indicate that CRH and UCN might play an immunoregulatory role in endometriotic sites by affecting reproductive functions such as decidualization and implantation of women with endometriosis.</description><subject>Analysis</subject><subject>Biology</subject><subject>Biopsy</subject><subject>Case-Control Studies</subject><subject>Corticotropin-releasing hormone</subject><subject>Corticotropin-Releasing Hormone - genetics</subject><subject>Corticotropin-Releasing Hormone - metabolism</subject><subject>Cytokines</subject><subject>Endocrinology</subject><subject>Endometriosis</subject><subject>Endometriosis - genetics</subject><subject>Endometriosis - metabolism</subject><subject>Endometriosis - pathology</subject><subject>Endometrium</subject><subject>Endometrium - metabolism</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genetic aspects</subject><subject>Gynecology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunoregulation</subject><subject>Implantation</subject><subject>Infertility</subject><subject>Medical schools</subject><subject>Medicine</subject><subject>Menstruation</subject><subject>mRNA</subject><subject>Neurohormones</subject><subject>Neuropeptides</subject><subject>Obstetrics</subject><subject>Pain</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Polymerase chain reaction</subject><subject>Receptors</subject><subject>Receptors, Corticotropin-Releasing Hormone - genetics</subject><subject>Receptors, Corticotropin-Releasing Hormone - metabolism</subject><subject>Risk factors</subject><subject>Rodents</subject><subject>Urocortin</subject><subject>Urocortins - genetics</subject><subject>Urocortins - metabolism</subject><subject>Western blotting</subject><subject>Womens health</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk21v0zAQxyMEYmPwDRBEQkIgrcUPieO8QZrKwypNTCqMt9bFsVtXSZzZCRvfHqfNRoP2AvmFL-ff_Z2780XRS4zmmGb4w9b2roFq3tpGzRFihGL6KDrGOSUzRhB9fGAfRc-83yKUUs7Y0-iIUEY5oulx5D8ZrZVTTWegitVt65T3xjax1fFidX4aXy2-nQ7WCsfQlDuLxKaJVd_Z1sidU8m9rZrS1qpzpq-H-Jvw0cQ3ptv8PbHe-OfREw2VVy_G_SS6-vL5x-J8dnH5dbk4u5hJlpNupnSKJKKKyYSH3FKdYwQggecJS8qMQCY1wVlGSpbnhJacljQtOOEkA8QA6En0eq_bVtaLsV5eYJownGaU8UAs90RpYStaZ2pwv4UFI3YO69YCXGdkpQQUhBcaClDAkhQlkGqVp4TnOMdJQdOg9XG8rS9qVcpQUgfVRHR60piNWNtfIvQiycgg8G4UcPa6V74TtfFSVRU0yva7_-ZJnoe-BfTNP-jD2Y3UGkICptE23CsHUXGWZBwRSvFAzR-gwipVbWR4XNoE_yTg_SQgMJ267dbQey-W31f_z17-nLJvD9iNgqrbeFv1XXiOfgome1A6671T-r7IGIlhNu6qIYbZEONshLBXhw26D7obBvoHSoAHbA</recordid><startdate>20130424</startdate><enddate>20130424</enddate><creator>Vergetaki, Aikaterini</creator><creator>Jeschke, Udo</creator><creator>Vrekoussis, Thomas</creator><creator>Taliouri, Eirini</creator><creator>Sabatini, Luca</creator><creator>Papakonstanti, Evangelia A</creator><creator>Makrigiannakis, Antonis</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130424</creationdate><title>Differential expression of CRH, UCN, CRHR1 and CRHR2 in eutopic and ectopic endometrium of women with endometriosis</title><author>Vergetaki, Aikaterini ; Jeschke, Udo ; Vrekoussis, Thomas ; Taliouri, Eirini ; Sabatini, Luca ; Papakonstanti, Evangelia A ; Makrigiannakis, Antonis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-ef50c03e6c483135f910aaca89464d72a7cf21772d69923d83d35b82827a06aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analysis</topic><topic>Biology</topic><topic>Biopsy</topic><topic>Case-Control Studies</topic><topic>Corticotropin-releasing hormone</topic><topic>Corticotropin-Releasing Hormone - genetics</topic><topic>Corticotropin-Releasing Hormone - metabolism</topic><topic>Cytokines</topic><topic>Endocrinology</topic><topic>Endometriosis</topic><topic>Endometriosis - genetics</topic><topic>Endometriosis - metabolism</topic><topic>Endometriosis - pathology</topic><topic>Endometrium</topic><topic>Endometrium - metabolism</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genetic aspects</topic><topic>Gynecology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunoregulation</topic><topic>Implantation</topic><topic>Infertility</topic><topic>Medical schools</topic><topic>Medicine</topic><topic>Menstruation</topic><topic>mRNA</topic><topic>Neurohormones</topic><topic>Neuropeptides</topic><topic>Obstetrics</topic><topic>Pain</topic><topic>Pathogenesis</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Polymerase chain reaction</topic><topic>Receptors</topic><topic>Receptors, Corticotropin-Releasing Hormone - genetics</topic><topic>Receptors, Corticotropin-Releasing Hormone - metabolism</topic><topic>Risk factors</topic><topic>Rodents</topic><topic>Urocortin</topic><topic>Urocortins - genetics</topic><topic>Urocortins - metabolism</topic><topic>Western blotting</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vergetaki, Aikaterini</creatorcontrib><creatorcontrib>Jeschke, Udo</creatorcontrib><creatorcontrib>Vrekoussis, Thomas</creatorcontrib><creatorcontrib>Taliouri, Eirini</creatorcontrib><creatorcontrib>Sabatini, Luca</creatorcontrib><creatorcontrib>Papakonstanti, Evangelia A</creatorcontrib><creatorcontrib>Makrigiannakis, Antonis</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vergetaki, Aikaterini</au><au>Jeschke, Udo</au><au>Vrekoussis, Thomas</au><au>Taliouri, Eirini</au><au>Sabatini, Luca</au><au>Papakonstanti, Evangelia A</au><au>Makrigiannakis, Antonis</au><au>Critchley, Hilary OD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of CRH, UCN, CRHR1 and CRHR2 in eutopic and ectopic endometrium of women with endometriosis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-04-24</date><risdate>2013</risdate><volume>8</volume><issue>4</issue><spage>e62313</spage><epage>e62313</epage><pages>e62313-e62313</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Endometriosis is considered as a benign aseptic inflammatory disease, characterised by the presence of ectopic endometrium-like tissue. Its symptoms (mostly pain and infertility) are reported as constant stressors. Corticotropin releasing hormone (CRH) and urocortin (UCN) are neuropeptides, strongly related to stress and inflammation. The effects of CRH and UCN are mediated through CRHR1 and CRHR2 receptors which are implicated in several reproductive functions acting as inflammatory components. However, the involvement of these molecules to endometriosis remains unknown. The aim of this study was to examine the expression of CRHR1 and CRHR2 in endometriotic sites and to compare the expression of CRHR1 and CRHR2 in eutopic endometrium of endometriotic women to that of healthy women. We further compared the expression of CRH, UCN, CRHR1 and CRHR2 in ectopic endometrium to that in eutopic endometrium of women with endometriosis. Endometrial biopsy specimens were taken from healthy women (10 patients) and endometrial and endometriotic biopsy specimens were taken from women with endometriosis (16 patients). Τhe expression of CRH, UCN, CRHR1, and CRHR2 was tested via RT-PCR, immunohistochemistry and Western blotting. This study shows for the first time that CRH and UCN receptor subtypes CRHR1β and CRHR2α are expressed in endometriotic sites and that they are more strongly expressed (p<0.01) in eutopic endometrium of women with endometriosis compared to healthy women endometrium at the mRNA and protein level. CRH, UCN, CRHR1 and CRHR2 mRNA were also more highly expressed in ectopic rather than eutopic endometrium (CRH, UCN, CRHR2α: p<0.01, CRHR1β: p<0.05) and protein (CRH and UCN: p<0.05, CRHR1 and CRHR2: p<0.01) in women with endometriosis. These data indicate that CRH and UCN might play an immunoregulatory role in endometriotic sites by affecting reproductive functions such as decidualization and implantation of women with endometriosis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23638035</pmid><doi>10.1371/journal.pone.0062313</doi><tpages>e62313</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-04, Vol.8 (4), p.e62313-e62313 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1346157368 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Analysis Biology Biopsy Case-Control Studies Corticotropin-releasing hormone Corticotropin-Releasing Hormone - genetics Corticotropin-Releasing Hormone - metabolism Cytokines Endocrinology Endometriosis Endometriosis - genetics Endometriosis - metabolism Endometriosis - pathology Endometrium Endometrium - metabolism Female Gene expression Gene Expression Regulation Genetic aspects Gynecology Humans Immunohistochemistry Immunoregulation Implantation Infertility Medical schools Medicine Menstruation mRNA Neurohormones Neuropeptides Obstetrics Pain Pathogenesis Patients Physiological aspects Polymerase chain reaction Receptors Receptors, Corticotropin-Releasing Hormone - genetics Receptors, Corticotropin-Releasing Hormone - metabolism Risk factors Rodents Urocortin Urocortins - genetics Urocortins - metabolism Western blotting Womens health |
| title | Differential expression of CRH, UCN, CRHR1 and CRHR2 in eutopic and ectopic endometrium of women with endometriosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T18%3A38%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20expression%20of%20CRH,%20UCN,%20CRHR1%20and%20CRHR2%20in%20eutopic%20and%20ectopic%20endometrium%20of%20women%20with%20endometriosis&rft.jtitle=PloS%20one&rft.au=Vergetaki,%20Aikaterini&rft.date=2013-04-24&rft.volume=8&rft.issue=4&rft.spage=e62313&rft.epage=e62313&rft.pages=e62313-e62313&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0062313&rft_dat=%3Cgale_plos_%3EA478023318%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1346157368&rft_id=info:pmid/23638035&rft_galeid=A478023318&rft_doaj_id=oai_doaj_org_article_ab28bfabaea64504a5fe952891914b35&rfr_iscdi=true |