Expression of ethanol-induced behavioral sensitization is associated with alteration of chromatin remodeling in mice
Ethanol-induced behavioral sensitization (EIBS) is proposed to play a role in early and recurring steps of addiction. EIBS does not occur uniformly in all animals even from the same inbred strain. Since recent data demonstrate that epigenetic mechanisms are likely to be involved in the development a...
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| description | Ethanol-induced behavioral sensitization (EIBS) is proposed to play a role in early and recurring steps of addiction. EIBS does not occur uniformly in all animals even from the same inbred strain. Since recent data demonstrate that epigenetic mechanisms are likely to be involved in the development and the persistence of ethanol-related behaviors, we explored the involvement of epigenetic mechanisms in ethanol response after EIBS development.
DBA/2J mice were i.p. injected with saline or ethanol (2 g/kg) once a day for 10 consecutive days. At day 17, ethanol-treated mice were split in resistant and sensitized groups. Brains were then removed 30 min after a saline or 2 g/kg ethanol challenge to assess i) gene expression using PCR array targeting 84 epigenetic-related genes and ii) histone deacetylases (HDAC), histone acetylases (HAT) and DNA methyltransferases (DNMT) activities as well as H4K12 acetylation.
Acute ethanol administration decreased dnmt1, esco2 and rps6ka5 genes expression. These genes were similarly altered in sensitized but not in resistant mice after an ethanol challenge, suggesting that resistant mice were tolerant to the transcriptional outcomes of an ethanol challenge. Whereas global HAT or DNMT activity was not affected, global HDAC activity was reduced after an acute ethanol injection. HDAC inhibition occurred in all ethanol-treated mice but with a lesser extent in sensitized animals. As a consequence, H4 acetylation was specifically potentiated in the core of the Nac proportionally to the striatal HDAC activity decrease.
The present study highlights that the contrasted behavioral response to an ethanol challenge between resistant and sensitized mice may be mediated by epigenetic mechanisms occurring specifically in the striatum. Here we show that vulnerability to ethanol dependence and relapse could be, at least in part, due to individual variability in acute ethanol-induced epigenetic response. |
| doi_str_mv | 10.1371/journal.pone.0047527 |
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DBA/2J mice were i.p. injected with saline or ethanol (2 g/kg) once a day for 10 consecutive days. At day 17, ethanol-treated mice were split in resistant and sensitized groups. Brains were then removed 30 min after a saline or 2 g/kg ethanol challenge to assess i) gene expression using PCR array targeting 84 epigenetic-related genes and ii) histone deacetylases (HDAC), histone acetylases (HAT) and DNA methyltransferases (DNMT) activities as well as H4K12 acetylation.
Acute ethanol administration decreased dnmt1, esco2 and rps6ka5 genes expression. These genes were similarly altered in sensitized but not in resistant mice after an ethanol challenge, suggesting that resistant mice were tolerant to the transcriptional outcomes of an ethanol challenge. Whereas global HAT or DNMT activity was not affected, global HDAC activity was reduced after an acute ethanol injection. HDAC inhibition occurred in all ethanol-treated mice but with a lesser extent in sensitized animals. As a consequence, H4 acetylation was specifically potentiated in the core of the Nac proportionally to the striatal HDAC activity decrease.
The present study highlights that the contrasted behavioral response to an ethanol challenge between resistant and sensitized mice may be mediated by epigenetic mechanisms occurring specifically in the striatum. Here we show that vulnerability to ethanol dependence and relapse could be, at least in part, due to individual variability in acute ethanol-induced epigenetic response.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0047527</identifier><identifier>PMID: 23110077</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetylation ; Acetylation - drug effects ; Addictions ; Alcoholism ; Animals ; Behavior, Animal - drug effects ; Biology ; Botia ; Brain research ; Chemistry ; Chromatin ; Chromatin Assembly and Disassembly - drug effects ; Chromatin Assembly and Disassembly - genetics ; Chromatin remodeling ; Cocaine ; Deoxyribonucleic acid ; DNA ; DNMT1 protein ; Dopamine ; Drug dependence ; Epigenetic inheritance ; Epigenetics ; Ethanol ; Ethanol - metabolism ; Ethanol - toxicity ; Female ; Gene expression ; Genes ; Genetically modified mice ; Health aspects ; Histone Acetyltransferases - genetics ; Histone deacetylase ; Histone Deacetylases - genetics ; Histones - drug effects ; Histones - metabolism ; Inbreeding ; Kinases ; Life Sciences ; Medicine ; Methamphetamine ; Methyltransferases ; Mice ; Neostriatum ; Neurons and Cognition ; Polymerase Chain Reaction ; Saline solutions ; Transcription</subject><ispartof>PloS one, 2012-10, Vol.7 (10), p.e47527</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Botia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2012 Botia et al 2012 Botia et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c729t-8ad5163af1e913c0ba68e6505f0eb2ef49d504719d087fe3be04415ab82185e43</citedby><orcidid>0000-0002-9788-0918 ; 0000-0002-7773-5836</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478273/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3478273/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23110077$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-00759738$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Choi, Doo-Sup</contributor><creatorcontrib>Botia, Béatrice</creatorcontrib><creatorcontrib>Legastelois, Rémi</creatorcontrib><creatorcontrib>Alaux-Cantin, Stéphanie</creatorcontrib><creatorcontrib>Naassila, Mickaël</creatorcontrib><title>Expression of ethanol-induced behavioral sensitization is associated with alteration of chromatin remodeling in mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Ethanol-induced behavioral sensitization (EIBS) is proposed to play a role in early and recurring steps of addiction. EIBS does not occur uniformly in all animals even from the same inbred strain. Since recent data demonstrate that epigenetic mechanisms are likely to be involved in the development and the persistence of ethanol-related behaviors, we explored the involvement of epigenetic mechanisms in ethanol response after EIBS development.
DBA/2J mice were i.p. injected with saline or ethanol (2 g/kg) once a day for 10 consecutive days. At day 17, ethanol-treated mice were split in resistant and sensitized groups. Brains were then removed 30 min after a saline or 2 g/kg ethanol challenge to assess i) gene expression using PCR array targeting 84 epigenetic-related genes and ii) histone deacetylases (HDAC), histone acetylases (HAT) and DNA methyltransferases (DNMT) activities as well as H4K12 acetylation.
Acute ethanol administration decreased dnmt1, esco2 and rps6ka5 genes expression. These genes were similarly altered in sensitized but not in resistant mice after an ethanol challenge, suggesting that resistant mice were tolerant to the transcriptional outcomes of an ethanol challenge. Whereas global HAT or DNMT activity was not affected, global HDAC activity was reduced after an acute ethanol injection. HDAC inhibition occurred in all ethanol-treated mice but with a lesser extent in sensitized animals. As a consequence, H4 acetylation was specifically potentiated in the core of the Nac proportionally to the striatal HDAC activity decrease.
The present study highlights that the contrasted behavioral response to an ethanol challenge between resistant and sensitized mice may be mediated by epigenetic mechanisms occurring specifically in the striatum. 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EIBS does not occur uniformly in all animals even from the same inbred strain. Since recent data demonstrate that epigenetic mechanisms are likely to be involved in the development and the persistence of ethanol-related behaviors, we explored the involvement of epigenetic mechanisms in ethanol response after EIBS development.
DBA/2J mice were i.p. injected with saline or ethanol (2 g/kg) once a day for 10 consecutive days. At day 17, ethanol-treated mice were split in resistant and sensitized groups. Brains were then removed 30 min after a saline or 2 g/kg ethanol challenge to assess i) gene expression using PCR array targeting 84 epigenetic-related genes and ii) histone deacetylases (HDAC), histone acetylases (HAT) and DNA methyltransferases (DNMT) activities as well as H4K12 acetylation.
Acute ethanol administration decreased dnmt1, esco2 and rps6ka5 genes expression. These genes were similarly altered in sensitized but not in resistant mice after an ethanol challenge, suggesting that resistant mice were tolerant to the transcriptional outcomes of an ethanol challenge. Whereas global HAT or DNMT activity was not affected, global HDAC activity was reduced after an acute ethanol injection. HDAC inhibition occurred in all ethanol-treated mice but with a lesser extent in sensitized animals. As a consequence, H4 acetylation was specifically potentiated in the core of the Nac proportionally to the striatal HDAC activity decrease.
The present study highlights that the contrasted behavioral response to an ethanol challenge between resistant and sensitized mice may be mediated by epigenetic mechanisms occurring specifically in the striatum. Here we show that vulnerability to ethanol dependence and relapse could be, at least in part, due to individual variability in acute ethanol-induced epigenetic response.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23110077</pmid><doi>10.1371/journal.pone.0047527</doi><tpages>e47527</tpages><orcidid>https://orcid.org/0000-0002-9788-0918</orcidid><orcidid>https://orcid.org/0000-0002-7773-5836</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acetylation Acetylation - drug effects Addictions Alcoholism Animals Behavior, Animal - drug effects Biology Botia Brain research Chemistry Chromatin Chromatin Assembly and Disassembly - drug effects Chromatin Assembly and Disassembly - genetics Chromatin remodeling Cocaine Deoxyribonucleic acid DNA DNMT1 protein Dopamine Drug dependence Epigenetic inheritance Epigenetics Ethanol Ethanol - metabolism Ethanol - toxicity Female Gene expression Genes Genetically modified mice Health aspects Histone Acetyltransferases - genetics Histone deacetylase Histone Deacetylases - genetics Histones - drug effects Histones - metabolism Inbreeding Kinases Life Sciences Medicine Methamphetamine Methyltransferases Mice Neostriatum Neurons and Cognition Polymerase Chain Reaction Saline solutions Transcription |
| title | Expression of ethanol-induced behavioral sensitization is associated with alteration of chromatin remodeling in mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T22%3A28%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20ethanol-induced%20behavioral%20sensitization%20is%20associated%20with%20alteration%20of%20chromatin%20remodeling%20in%20mice&rft.jtitle=PloS%20one&rft.au=Botia,%20B%C3%A9atrice&rft.date=2012-10-22&rft.volume=7&rft.issue=10&rft.spage=e47527&rft.pages=e47527-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0047527&rft_dat=%3Cgale_plos_%3EA498251471%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1345548515&rft_id=info:pmid/23110077&rft_galeid=A498251471&rft_doaj_id=oai_doaj_org_article_a13c9e6ae0bd4ceca83455d80cd202e2&rfr_iscdi=true |