Label-free enrichment of adrenal cortical progenitor cells using inertial microfluidics
Passive and label-free isolation of viable target cells based on intrinsic biophysical cellular properties would allow for cost savings in applications where molecular biomarkers are known as well as potentially enable the separation of cells with little-to-no known molecular biomarkers. We have dem...
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| Veröffentlicht in: | PloS one 2012-10, Vol.7 (10), p.e46550-e46550 |
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| creator | Hur, Soojung Claire Brinckerhoff, Tatiana Z Walthers, Christopher M Dunn, James C Y Di Carlo, Dino |
| description | Passive and label-free isolation of viable target cells based on intrinsic biophysical cellular properties would allow for cost savings in applications where molecular biomarkers are known as well as potentially enable the separation of cells with little-to-no known molecular biomarkers. We have demonstrated the purification of adrenal cortical progenitor cells from digestions of murine adrenal glands utilizing hydrodynamic inertial lift forces that single cells and multicellular clusters differentially experience as they flow through a microchannel. Fluorescence staining, along with gene expression measurements, confirmed that populations of cells collected in different outlets were distinct from one another. Furthermore, primary murine cells processed through the device remained highly viable and could be cultured for 10 days in vitro. The proposed target cell isolation technique can provide a practical means to collect significant quantities of viable intact cells required to translate stem cell biology to regenerative medicine in a simple label-free manner. |
| doi_str_mv | 10.1371/journal.pone.0046550 |
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We have demonstrated the purification of adrenal cortical progenitor cells from digestions of murine adrenal glands utilizing hydrodynamic inertial lift forces that single cells and multicellular clusters differentially experience as they flow through a microchannel. Fluorescence staining, along with gene expression measurements, confirmed that populations of cells collected in different outlets were distinct from one another. Furthermore, primary murine cells processed through the device remained highly viable and could be cultured for 10 days in vitro. The proposed target cell isolation technique can provide a practical means to collect significant quantities of viable intact cells required to translate stem cell biology to regenerative medicine in a simple label-free manner.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0046550</identifier><identifier>PMID: 23056341</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adrenal Cortex - cytology ; Adrenal Cortex - metabolism ; Adrenal glands ; Animals ; Bioengineering ; Biology ; Biomarkers ; Biomarkers - metabolism ; Cells (biology) ; Cortex ; Engineering ; Female ; Fluorescence ; Gene expression ; Hormones ; Mentha piperita ; Mice ; Mice, Inbred C57BL ; Microfluidics ; Purification ; Regenerative medicine ; Stem cells ; Stem Cells - cytology ; Stem Cells - metabolism ; Technology application</subject><ispartof>PloS one, 2012-10, Vol.7 (10), p.e46550-e46550</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Hur et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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We have demonstrated the purification of adrenal cortical progenitor cells from digestions of murine adrenal glands utilizing hydrodynamic inertial lift forces that single cells and multicellular clusters differentially experience as they flow through a microchannel. Fluorescence staining, along with gene expression measurements, confirmed that populations of cells collected in different outlets were distinct from one another. Furthermore, primary murine cells processed through the device remained highly viable and could be cultured for 10 days in vitro. 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We have demonstrated the purification of adrenal cortical progenitor cells from digestions of murine adrenal glands utilizing hydrodynamic inertial lift forces that single cells and multicellular clusters differentially experience as they flow through a microchannel. Fluorescence staining, along with gene expression measurements, confirmed that populations of cells collected in different outlets were distinct from one another. Furthermore, primary murine cells processed through the device remained highly viable and could be cultured for 10 days in vitro. The proposed target cell isolation technique can provide a practical means to collect significant quantities of viable intact cells required to translate stem cell biology to regenerative medicine in a simple label-free manner.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23056341</pmid><doi>10.1371/journal.pone.0046550</doi><tpages>e46550</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adrenal Cortex - cytology Adrenal Cortex - metabolism Adrenal glands Animals Bioengineering Biology Biomarkers Biomarkers - metabolism Cells (biology) Cortex Engineering Female Fluorescence Gene expression Hormones Mentha piperita Mice Mice, Inbred C57BL Microfluidics Purification Regenerative medicine Stem cells Stem Cells - cytology Stem Cells - metabolism Technology application |
| title | Label-free enrichment of adrenal cortical progenitor cells using inertial microfluidics |
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