MicroRNAome comparison between intramuscular and subcutaneous vascular stem cell adipogenesis
As an important factor affecting meat quality, intramuscular fat (IMF) content is a topic of worldwide concern. Emerging evidences indicate that microRNAs play important roles in adipocyte differentiation. However, miRNAome has neither been studied during porcine intramuscular preadipocyte different...
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| creator | Guo, Yunxue Mo, Delin Zhang, Yue Zhang, Yun Cong, Peiqing Xiao, Shuqi He, Zuyong Liu, Xiaohong Chen, Yaosheng |
| description | As an important factor affecting meat quality, intramuscular fat (IMF) content is a topic of worldwide concern. Emerging evidences indicate that microRNAs play important roles in adipocyte differentiation. However, miRNAome has neither been studied during porcine intramuscular preadipocyte differentiation, nor compared with subcutaneous preadipocytes. The objectives of this study were to identify porcine miRNAs involved in adipogenesis in primary preadipocytes, and to determine whether intramuscular and subcutaneous adipocytes differ in the expression and regulation of miRNAs.
miRNAomes in primary intramuscular and subcutaneous adipocytes during differentiation were first sequenced using the Solexa deep sequencing method. The sequences and relative expression levels of 224 known (98.2% in miRbase 18.0) and 280 potential porcine miRNAs were identified. Fifty-four of them changed in similar pattern between intramuscular vascular stem cells (IVSC) and subcutaneous vascular stem cells (SVSC) differentiation, such as miR-210, miR-10b and miR-99a. Expression levels of 10 miRNAs were reversely up-or down-regulated between IVSC and SVSC differentiation, 19 were up-or down-regulated only during IVSC differentiation and 55 only during SVSC differentiation. Additionally, 30 miRNAs showed fat-depot specific expression pattern (24 in cells of intramuscular origin and 6 in cells of subcutaneous origin). These adipogenesis-related miRNAs mainly functioned by targeting similar pathways in adipogenesis, obesity and syndrome.
Comparison of miRNAomes in IVSC and SVSC during differentiation revealed that many different miRNAs are involved in adipogenesis, and they regulate SVSC and IVSC differentiation through similar pathways. These miRNAs may serve as biomarkers or targets for enhancing IMF content, and uncovering their function in IMF development will be of great value in the near future. |
| doi_str_mv | 10.1371/journal.pone.0045410 |
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miRNAomes in primary intramuscular and subcutaneous adipocytes during differentiation were first sequenced using the Solexa deep sequencing method. The sequences and relative expression levels of 224 known (98.2% in miRbase 18.0) and 280 potential porcine miRNAs were identified. Fifty-four of them changed in similar pattern between intramuscular vascular stem cells (IVSC) and subcutaneous vascular stem cells (SVSC) differentiation, such as miR-210, miR-10b and miR-99a. Expression levels of 10 miRNAs were reversely up-or down-regulated between IVSC and SVSC differentiation, 19 were up-or down-regulated only during IVSC differentiation and 55 only during SVSC differentiation. Additionally, 30 miRNAs showed fat-depot specific expression pattern (24 in cells of intramuscular origin and 6 in cells of subcutaneous origin). These adipogenesis-related miRNAs mainly functioned by targeting similar pathways in adipogenesis, obesity and syndrome.
Comparison of miRNAomes in IVSC and SVSC during differentiation revealed that many different miRNAs are involved in adipogenesis, and they regulate SVSC and IVSC differentiation through similar pathways. These miRNAs may serve as biomarkers or targets for enhancing IMF content, and uncovering their function in IMF development will be of great value in the near future.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0045410</identifier><identifier>PMID: 23028990</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipocytes ; Adipogenesis ; Adipogenesis - genetics ; Adipogenesis - physiology ; Agriculture ; Animals ; Biological markers ; Biology ; Biomarkers ; Biosynthesis ; Blotting, Western ; Cattle ; Cell Differentiation - physiology ; Cells, Cultured ; Diabetes ; Differentiation ; Health aspects ; Hogs ; Insulin ; Laboratories ; Life sciences ; Lipids ; Meat ; Medicine ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Muscles - cytology ; Musculoskeletal system ; Obesity ; Physiological aspects ; Polymerase Chain Reaction ; Preadipocytes ; Proteins ; Stem cell transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - metabolism ; Subcutaneous Tissue ; Swine ; Triglycerides</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e45410-e45410</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Guo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Guo et al 2012 Guo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d8de1a52b5540a5cebee23590924da98c73d50a2cda6a43f5be443047ade4f23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447870/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447870/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23028990$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Watson, Michael</contributor><creatorcontrib>Guo, Yunxue</creatorcontrib><creatorcontrib>Mo, Delin</creatorcontrib><creatorcontrib>Zhang, Yue</creatorcontrib><creatorcontrib>Zhang, Yun</creatorcontrib><creatorcontrib>Cong, Peiqing</creatorcontrib><creatorcontrib>Xiao, Shuqi</creatorcontrib><creatorcontrib>He, Zuyong</creatorcontrib><creatorcontrib>Liu, Xiaohong</creatorcontrib><creatorcontrib>Chen, Yaosheng</creatorcontrib><title>MicroRNAome comparison between intramuscular and subcutaneous vascular stem cell adipogenesis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>As an important factor affecting meat quality, intramuscular fat (IMF) content is a topic of worldwide concern. Emerging evidences indicate that microRNAs play important roles in adipocyte differentiation. However, miRNAome has neither been studied during porcine intramuscular preadipocyte differentiation, nor compared with subcutaneous preadipocytes. The objectives of this study were to identify porcine miRNAs involved in adipogenesis in primary preadipocytes, and to determine whether intramuscular and subcutaneous adipocytes differ in the expression and regulation of miRNAs.
miRNAomes in primary intramuscular and subcutaneous adipocytes during differentiation were first sequenced using the Solexa deep sequencing method. The sequences and relative expression levels of 224 known (98.2% in miRbase 18.0) and 280 potential porcine miRNAs were identified. Fifty-four of them changed in similar pattern between intramuscular vascular stem cells (IVSC) and subcutaneous vascular stem cells (SVSC) differentiation, such as miR-210, miR-10b and miR-99a. Expression levels of 10 miRNAs were reversely up-or down-regulated between IVSC and SVSC differentiation, 19 were up-or down-regulated only during IVSC differentiation and 55 only during SVSC differentiation. Additionally, 30 miRNAs showed fat-depot specific expression pattern (24 in cells of intramuscular origin and 6 in cells of subcutaneous origin). These adipogenesis-related miRNAs mainly functioned by targeting similar pathways in adipogenesis, obesity and syndrome.
Comparison of miRNAomes in IVSC and SVSC during differentiation revealed that many different miRNAs are involved in adipogenesis, and they regulate SVSC and IVSC differentiation through similar pathways. These miRNAs may serve as biomarkers or targets for enhancing IMF content, and uncovering their function in IMF development will be of great value in the near future.</description><subject>Adipocytes</subject><subject>Adipogenesis</subject><subject>Adipogenesis - genetics</subject><subject>Adipogenesis - physiology</subject><subject>Agriculture</subject><subject>Animals</subject><subject>Biological markers</subject><subject>Biology</subject><subject>Biomarkers</subject><subject>Biosynthesis</subject><subject>Blotting, Western</subject><subject>Cattle</subject><subject>Cell Differentiation - physiology</subject><subject>Cells, Cultured</subject><subject>Diabetes</subject><subject>Differentiation</subject><subject>Health aspects</subject><subject>Hogs</subject><subject>Insulin</subject><subject>Laboratories</subject><subject>Life sciences</subject><subject>Lipids</subject><subject>Meat</subject><subject>Medicine</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - 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Emerging evidences indicate that microRNAs play important roles in adipocyte differentiation. However, miRNAome has neither been studied during porcine intramuscular preadipocyte differentiation, nor compared with subcutaneous preadipocytes. The objectives of this study were to identify porcine miRNAs involved in adipogenesis in primary preadipocytes, and to determine whether intramuscular and subcutaneous adipocytes differ in the expression and regulation of miRNAs.
miRNAomes in primary intramuscular and subcutaneous adipocytes during differentiation were first sequenced using the Solexa deep sequencing method. The sequences and relative expression levels of 224 known (98.2% in miRbase 18.0) and 280 potential porcine miRNAs were identified. Fifty-four of them changed in similar pattern between intramuscular vascular stem cells (IVSC) and subcutaneous vascular stem cells (SVSC) differentiation, such as miR-210, miR-10b and miR-99a. Expression levels of 10 miRNAs were reversely up-or down-regulated between IVSC and SVSC differentiation, 19 were up-or down-regulated only during IVSC differentiation and 55 only during SVSC differentiation. Additionally, 30 miRNAs showed fat-depot specific expression pattern (24 in cells of intramuscular origin and 6 in cells of subcutaneous origin). These adipogenesis-related miRNAs mainly functioned by targeting similar pathways in adipogenesis, obesity and syndrome.
Comparison of miRNAomes in IVSC and SVSC during differentiation revealed that many different miRNAs are involved in adipogenesis, and they regulate SVSC and IVSC differentiation through similar pathways. These miRNAs may serve as biomarkers or targets for enhancing IMF content, and uncovering their function in IMF development will be of great value in the near future.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23028990</pmid><doi>10.1371/journal.pone.0045410</doi><tpages>e45410</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipocytes Adipogenesis Adipogenesis - genetics Adipogenesis - physiology Agriculture Animals Biological markers Biology Biomarkers Biosynthesis Blotting, Western Cattle Cell Differentiation - physiology Cells, Cultured Diabetes Differentiation Health aspects Hogs Insulin Laboratories Life sciences Lipids Meat Medicine MicroRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Muscles - cytology Musculoskeletal system Obesity Physiological aspects Polymerase Chain Reaction Preadipocytes Proteins Stem cell transplantation Stem cells Stem Cells - cytology Stem Cells - metabolism Subcutaneous Tissue Swine Triglycerides |
| title | MicroRNAome comparison between intramuscular and subcutaneous vascular stem cell adipogenesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T11%3A20%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MicroRNAome%20comparison%20between%20intramuscular%20and%20subcutaneous%20vascular%20stem%20cell%20adipogenesis&rft.jtitle=PloS%20one&rft.au=Guo,%20Yunxue&rft.date=2012-09-20&rft.volume=7&rft.issue=9&rft.spage=e45410&rft.epage=e45410&rft.pages=e45410-e45410&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0045410&rft_dat=%3Cgale_plos_%3EA498259095%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1344598767&rft_id=info:pmid/23028990&rft_galeid=A498259095&rft_doaj_id=oai_doaj_org_article_138ceb63f9ad4bc0aaf74afcad9393ad&rfr_iscdi=true |