Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent
The biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumula...
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| description | The biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumulation ranging from 49.7 to 68.9% on cell dry weight (CDW) basis were observed when fatty acids ranging from octanoic acid (C(8:0)) to oleic acid (C(18:1)) were used as sole carbon and energy source. Molecular weight of the polymer was found to be ranging from 55.7 to 77.7 kDa. Depending on the type of fatty acid used, the (1)H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability, having a thermal degradation temperature (T(d)) of 264.6 to 318.8 (± 0.2) (o)C, melting temperature (T(m)) of 43. (± 0.2) (o)C, glass transition temperature (T(g)) of -1.0 (± 0.2) (o)C and apparent melting enthalpy of fusion (ΔH(f)) of 100.9 (± 0.1) J g(-1). |
| doi_str_mv | 10.1371/journal.pone.0045214 |
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The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumulation ranging from 49.7 to 68.9% on cell dry weight (CDW) basis were observed when fatty acids ranging from octanoic acid (C(8:0)) to oleic acid (C(18:1)) were used as sole carbon and energy source. Molecular weight of the polymer was found to be ranging from 55.7 to 77.7 kDa. Depending on the type of fatty acid used, the (1)H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability, having a thermal degradation temperature (T(d)) of 264.6 to 318.8 (± 0.2) (o)C, melting temperature (T(m)) of 43. (± 0.2) (o)C, glass transition temperature (T(g)) of -1.0 (± 0.2) (o)C and apparent melting enthalpy of fusion (ΔH(f)) of 100.9 (± 0.1) J g(-1).</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0045214</identifier><identifier>PMID: 23028854</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Arecaceae - microbiology ; Bacteria ; Biodegradation ; Biology ; Biomass ; Biosynthesis ; Carbon ; Carbon - metabolism ; Chemical analysis ; Deoxyribonucleic acid ; DNA ; Effluents ; Enthalpy ; Fatty acids ; Fatty Acids - analysis ; Fourier transforms ; Gas Chromatography-Mass Spectrometry ; Glass transition temperature ; Magnetic Resonance Spectroscopy ; Melting ; Microorganisms ; Molecular Conformation ; Molecular Weight ; Monomers ; NMR ; Nuclear magnetic resonance ; Octanoic acid ; Oleic acid ; Palm Oil ; Phase Transition ; Plant Oils - chemistry ; Polyhydroxyalkanoates ; Polyhydroxyalkanoates - biosynthesis ; Polyhydroxyalkanoates - isolation & purification ; Polymerization ; Polymers ; Pseudomonas ; Pseudomonas putida ; Pseudomonas putida - metabolism ; Science ; Temperature ; Temperature effects ; Thermal degradation ; Thermal stability ; Thermodynamics ; Transition temperatures ; Vegetable oils</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e45214-e45214</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Gumel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumulation ranging from 49.7 to 68.9% on cell dry weight (CDW) basis were observed when fatty acids ranging from octanoic acid (C(8:0)) to oleic acid (C(18:1)) were used as sole carbon and energy source. Molecular weight of the polymer was found to be ranging from 55.7 to 77.7 kDa. Depending on the type of fatty acid used, the (1)H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability, having a thermal degradation temperature (T(d)) of 264.6 to 318.8 (± 0.2) (o)C, melting temperature (T(m)) of 43. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gumel, Ahmad Mohammed</au><au>Annuar, Mohamad Suffian Mohamad</au><au>Heidelberg, Thorsten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-09-20</date><risdate>2012</risdate><volume>7</volume><issue>9</issue><spage>e45214</spage><epage>e45214</epage><pages>e45214-e45214</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The biosynthesis and characterization of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. The biosynthesis of mcl-PHA in this newly isolated microorganism follows a growth-associated trend. Mcl-PHA accumulation ranging from 49.7 to 68.9% on cell dry weight (CDW) basis were observed when fatty acids ranging from octanoic acid (C(8:0)) to oleic acid (C(18:1)) were used as sole carbon and energy source. Molecular weight of the polymer was found to be ranging from 55.7 to 77.7 kDa. Depending on the type of fatty acid used, the (1)H NMR and GCMSMS analyses of the chiral polymer showed a composition of even and odd carbon atom chain with monomer length of C4 to C14 with C8 and C10 as the principal monomers. No unsaturated monomer was detected. Thermo-chemical analyses showed the accumulated PHA to be semi-crystalline polymer with good thermal stability, having a thermal degradation temperature (T(d)) of 264.6 to 318.8 (± 0.2) (o)C, melting temperature (T(m)) of 43. (± 0.2) (o)C, glass transition temperature (T(g)) of -1.0 (± 0.2) (o)C and apparent melting enthalpy of fusion (ΔH(f)) of 100.9 (± 0.1) J g(-1).</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23028854</pmid><doi>10.1371/journal.pone.0045214</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Arecaceae - microbiology Bacteria Biodegradation Biology Biomass Biosynthesis Carbon Carbon - metabolism Chemical analysis Deoxyribonucleic acid DNA Effluents Enthalpy Fatty acids Fatty Acids - analysis Fourier transforms Gas Chromatography-Mass Spectrometry Glass transition temperature Magnetic Resonance Spectroscopy Melting Microorganisms Molecular Conformation Molecular Weight Monomers NMR Nuclear magnetic resonance Octanoic acid Oleic acid Palm Oil Phase Transition Plant Oils - chemistry Polyhydroxyalkanoates Polyhydroxyalkanoates - biosynthesis Polyhydroxyalkanoates - isolation & purification Polymerization Polymers Pseudomonas Pseudomonas putida Pseudomonas putida - metabolism Science Temperature Temperature effects Thermal degradation Thermal stability Thermodynamics Transition temperatures Vegetable oils |
| title | Biosynthesis and characterization of polyhydroxyalkanoates copolymers produced by Pseudomonas putida Bet001 isolated from palm oil mill effluent |
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