Isolation and characterization of multipotential mesenchymal cells from the mouse synovium
The human synovium contains mesenchymal stem cells (MSCs), which are multipotential non-hematopoietic progenitor cells that can differentiate into a variety of mesenchymal lineages and they may therefore be a candidate cell source for tissue repair. However, the molecular mechanisms by which this ca...
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| Veröffentlicht in: | PloS one 2012-09, Vol.7 (9), p.e45517-e45517 |
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| creator | Futami, Ippei Ishijima, Muneaki Kaneko, Haruka Tsuji, Kunikazu Ichikawa-Tomikawa, Naoki Sadatsuki, Ryo Muneta, Takeshi Arikawa-Hirasawa, Eri Sekiya, Ichiro Kaneko, Kazuo |
| description | The human synovium contains mesenchymal stem cells (MSCs), which are multipotential non-hematopoietic progenitor cells that can differentiate into a variety of mesenchymal lineages and they may therefore be a candidate cell source for tissue repair. However, the molecular mechanisms by which this can occur are still largely unknown. Mouse primary cell culture enables us to investigate the molecular mechanisms underlying various phenomena because it allows for relatively easy gene manipulation, which is indispensable for the molecular analysis. However, mouse synovial mesenchymal cells (SMCs) have not been established, although rabbit, cow, and rat SMCs are available, in addition to human MSCs. The aim of this study was to establish methods to harvest the synovium and to isolate and culture primary SMCs from mice. As the mouse SMCs were not able to be harvested and isolated using the same protocol for human, rat and rabbit SMCs, the protocol for humans was modified for SMCs from the Balb/c mouse knee joint. The mouse SMCs obtained showed superior proliferative potential, growth kinetics and colony formation compared to cells derived from muscle and bone marrow. They expressed PDGFRá and Sca-1 detected by flow cytometry, and showed an osteogenic, adipogenic and chondrogenic potential similar or superior to the cells derived from muscle and bone marrow by demonstrating in vitro osteogenesis, adipogenesis and chondrogenesis. In conclusion, we established a primary mouse synovial cell culture method. The cells derived from the mouse synovium demonstrated both the ability to proliferate and multipotentiality similar or superior to the cells derived from muscle and bone marrow. |
| doi_str_mv | 10.1371/journal.pone.0045517 |
| format | Article |
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However, the molecular mechanisms by which this can occur are still largely unknown. Mouse primary cell culture enables us to investigate the molecular mechanisms underlying various phenomena because it allows for relatively easy gene manipulation, which is indispensable for the molecular analysis. However, mouse synovial mesenchymal cells (SMCs) have not been established, although rabbit, cow, and rat SMCs are available, in addition to human MSCs. The aim of this study was to establish methods to harvest the synovium and to isolate and culture primary SMCs from mice. As the mouse SMCs were not able to be harvested and isolated using the same protocol for human, rat and rabbit SMCs, the protocol for humans was modified for SMCs from the Balb/c mouse knee joint. The mouse SMCs obtained showed superior proliferative potential, growth kinetics and colony formation compared to cells derived from muscle and bone marrow. They expressed PDGFRá and Sca-1 detected by flow cytometry, and showed an osteogenic, adipogenic and chondrogenic potential similar or superior to the cells derived from muscle and bone marrow by demonstrating in vitro osteogenesis, adipogenesis and chondrogenesis. In conclusion, we established a primary mouse synovial cell culture method. The cells derived from the mouse synovium demonstrated both the ability to proliferate and multipotentiality similar or superior to the cells derived from muscle and bone marrow.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0045517</identifier><identifier>PMID: 23029067</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipogenesis ; Adipogenesis - physiology ; Animals ; Biocompatibility ; Biology ; Biomedical materials ; Bone diseases ; Bone marrow ; Cell culture ; Cell Differentiation - physiology ; Cell Separation - methods ; Cells (biology) ; Chondrogenesis ; Chondrogenesis - physiology ; Cytometry ; Female ; Flow cytometry ; Ganglia ; Gene manipulation ; Genetic aspects ; Growth kinetics ; Hematopoietic stem cells ; Immunophenotyping ; Joint surgery ; Kinetics ; Knee ; Knee Joint - anatomy & histology ; Knee Joint - cytology ; Medicine ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Mesenchyme ; Mice ; Mice, Inbred BALB C ; Molecular modelling ; Muscles ; Musculoskeletal system ; Osteogenesis ; Osteogenesis - physiology ; Physiological aspects ; Primary Cell Culture ; Stem cell transplantation ; Stem cells ; Synovial Membrane - cytology ; Synovium ; Tissue engineering ; Transplants & implants ; University graduates</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e45517-e45517</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Futami et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Futami et al 2012 Futami et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-cec392c021cccb77a5c164cce56db8c4a4a6bc241252504b1e68fff7abefd23c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445493/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445493/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23029067$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Covas, Dimas Tadeu</contributor><creatorcontrib>Futami, Ippei</creatorcontrib><creatorcontrib>Ishijima, Muneaki</creatorcontrib><creatorcontrib>Kaneko, Haruka</creatorcontrib><creatorcontrib>Tsuji, Kunikazu</creatorcontrib><creatorcontrib>Ichikawa-Tomikawa, Naoki</creatorcontrib><creatorcontrib>Sadatsuki, Ryo</creatorcontrib><creatorcontrib>Muneta, Takeshi</creatorcontrib><creatorcontrib>Arikawa-Hirasawa, Eri</creatorcontrib><creatorcontrib>Sekiya, Ichiro</creatorcontrib><creatorcontrib>Kaneko, Kazuo</creatorcontrib><title>Isolation and characterization of multipotential mesenchymal cells from the mouse synovium</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The human synovium contains mesenchymal stem cells (MSCs), which are multipotential non-hematopoietic progenitor cells that can differentiate into a variety of mesenchymal lineages and they may therefore be a candidate cell source for tissue repair. 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They expressed PDGFRá and Sca-1 detected by flow cytometry, and showed an osteogenic, adipogenic and chondrogenic potential similar or superior to the cells derived from muscle and bone marrow by demonstrating in vitro osteogenesis, adipogenesis and chondrogenesis. In conclusion, we established a primary mouse synovial cell culture method. 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anatomy & histology</subject><subject>Knee Joint - cytology</subject><subject>Medicine</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchyme</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular modelling</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Osteogenesis</subject><subject>Osteogenesis - physiology</subject><subject>Physiological aspects</subject><subject>Primary Cell Culture</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Synovial Membrane - cytology</subject><subject>Synovium</subject><subject>Tissue engineering</subject><subject>Transplants & implants</subject><subject>University graduates</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlOeyMsix8DCwt-XXgT0jSZyZA2s0m6OP56U6e7TGUvJBcNp895T_LmnKJ4DsESYg7fbf0QeumWO9_rJQCEUsgfFKewxmjBEMAPj_YnxZMYtwBQXDH2uDhBGKAaMH5a_FxF72Syvi9l35ZqI4NUSQf7-xD0puwGl-zOJ90nK13Z6ah7tdl3ea-0c7E0wXdl2uiy80PUZdz3_sYO3dPikZEu6mfT96z4_vHDt4vPi8urT6uL88uF4rRKC6UVrpECCCqlGs4lVZARpTRlbVMpIolkjUIEIoooIA3UrDLGcNlo0yKs8Fnx8qC7cz6KyZYoICaEgppgnonVgWi93IpdsJ0Me-GlFX8DPqyFDMkqpwXkDWc1M7I1kLQcVhgyQyWnDEhV07Ha-6na0HS6VdmVIN1MdP6ntxux9jdiPA6pcRZ4MwkEfz3omERn42ik7HX2T0BQIQLyGWhGX_2D3n-7iVrLfAHbG5_rqlFUnJO6QgxhVGdqeQ-VV6s7q3IPGZvjs4S3s4TMJP0rreUQo1h9_fL_7NWPOfv6iN1o6dIm9-Awtlucg-QAquBjDNrcmQyBGEfg1g0xjoCYRiCnvTh-oLuk257HfwD3_gMY</recordid><startdate>20120918</startdate><enddate>20120918</enddate><creator>Futami, Ippei</creator><creator>Ishijima, Muneaki</creator><creator>Kaneko, Haruka</creator><creator>Tsuji, Kunikazu</creator><creator>Ichikawa-Tomikawa, Naoki</creator><creator>Sadatsuki, Ryo</creator><creator>Muneta, Takeshi</creator><creator>Arikawa-Hirasawa, Eri</creator><creator>Sekiya, Ichiro</creator><creator>Kaneko, Kazuo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120918</creationdate><title>Isolation and characterization of multipotential mesenchymal cells from the mouse synovium</title><author>Futami, Ippei ; Ishijima, Muneaki ; Kaneko, Haruka ; Tsuji, Kunikazu ; Ichikawa-Tomikawa, Naoki ; Sadatsuki, Ryo ; Muneta, Takeshi ; Arikawa-Hirasawa, Eri ; Sekiya, Ichiro ; Kaneko, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-cec392c021cccb77a5c164cce56db8c4a4a6bc241252504b1e68fff7abefd23c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adipogenesis</topic><topic>Adipogenesis - 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However, the molecular mechanisms by which this can occur are still largely unknown. Mouse primary cell culture enables us to investigate the molecular mechanisms underlying various phenomena because it allows for relatively easy gene manipulation, which is indispensable for the molecular analysis. However, mouse synovial mesenchymal cells (SMCs) have not been established, although rabbit, cow, and rat SMCs are available, in addition to human MSCs. The aim of this study was to establish methods to harvest the synovium and to isolate and culture primary SMCs from mice. As the mouse SMCs were not able to be harvested and isolated using the same protocol for human, rat and rabbit SMCs, the protocol for humans was modified for SMCs from the Balb/c mouse knee joint. The mouse SMCs obtained showed superior proliferative potential, growth kinetics and colony formation compared to cells derived from muscle and bone marrow. They expressed PDGFRá and Sca-1 detected by flow cytometry, and showed an osteogenic, adipogenic and chondrogenic potential similar or superior to the cells derived from muscle and bone marrow by demonstrating in vitro osteogenesis, adipogenesis and chondrogenesis. In conclusion, we established a primary mouse synovial cell culture method. The cells derived from the mouse synovium demonstrated both the ability to proliferate and multipotentiality similar or superior to the cells derived from muscle and bone marrow.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23029067</pmid><doi>10.1371/journal.pone.0045517</doi><tpages>e45517</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-09, Vol.7 (9), p.e45517-e45517 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adipogenesis Adipogenesis - physiology Animals Biocompatibility Biology Biomedical materials Bone diseases Bone marrow Cell culture Cell Differentiation - physiology Cell Separation - methods Cells (biology) Chondrogenesis Chondrogenesis - physiology Cytometry Female Flow cytometry Ganglia Gene manipulation Genetic aspects Growth kinetics Hematopoietic stem cells Immunophenotyping Joint surgery Kinetics Knee Knee Joint - anatomy & histology Knee Joint - cytology Medicine Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Mesenchyme Mice Mice, Inbred BALB C Molecular modelling Muscles Musculoskeletal system Osteogenesis Osteogenesis - physiology Physiological aspects Primary Cell Culture Stem cell transplantation Stem cells Synovial Membrane - cytology Synovium Tissue engineering Transplants & implants University graduates |
| title | Isolation and characterization of multipotential mesenchymal cells from the mouse synovium |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T23%3A39%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20characterization%20of%20multipotential%20mesenchymal%20cells%20from%20the%20mouse%20synovium&rft.jtitle=PloS%20one&rft.au=Futami,%20Ippei&rft.date=2012-09-18&rft.volume=7&rft.issue=9&rft.spage=e45517&rft.epage=e45517&rft.pages=e45517-e45517&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0045517&rft_dat=%3Cgale_plos_%3EA498262329%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1344509437&rft_id=info:pmid/23029067&rft_galeid=A498262329&rft_doaj_id=oai_doaj_org_article_17b7696fadf14d718316f5a7560ac95c&rfr_iscdi=true |