Overexpression of AtDREB1A causes a severe dwarf phenotype by decreasing endogenous gibberellin levels in soybean [Glycine max (L.) Merr]
Gibberellic acids (GAs) are plant hormones that play fundamental roles in plant growth and developmental processes. Previous studies have demonstrated that three key enzymes of GA20ox, GA3ox, and GA2ox are involved in GA biosynthesis. In this study, the Arabidopsis DREB1A gene driven by the CaMV 35S...
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| creator | Suo, Haicui Ma, Qibin Ye, Kaixin Yang, Cunyi Tang, Yujuan Hao, Juan Zhang, Zhanyuan J Chen, Mingluan Feng, Yuqi Nian, Hai |
| description | Gibberellic acids (GAs) are plant hormones that play fundamental roles in plant growth and developmental processes. Previous studies have demonstrated that three key enzymes of GA20ox, GA3ox, and GA2ox are involved in GA biosynthesis. In this study, the Arabidopsis DREB1A gene driven by the CaMV 35S promoter was introduced into soybean plants by Agrobacterium- mediated transformation. The results showed that the transgenic soybean plants exhibited a typical phenotype of GA-deficient mutants, such as severe dwarfism, small and dark-green leaves, and late flowering compared to those of the non-transgenic plants. The dwarfism phenotype was rescued by the application of exogenous GA(3) once a week for three weeks with the concentrations of 144 µM or three times in one week with the concentrations of 60 µM. Quantitative RT-PCR analysis revealed that the transcription levels of the GA synthase genes were higher in the transgenic soybean plants than those in controls, whereas GA-deactivated genes except GmGA2ox4 showed lower levels of expression. The transcript level of GmGA2ox4 encoding the only deactivation enzyme using C(20)-GAs as the substrates in soybean was dramatically enhanced in transgenic plants compared to that of wide type. Furthermore, the contents of endogenous bioactive GAs were significantly decreased in transgenic plants than those of wide type. The results suggested that AtDREB1A could cause dwarfism mediated by GA biosynthesis pathway in soybean. |
| doi_str_mv | 10.1371/journal.pone.0045568 |
| format | Article |
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Merr]</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>Public Library of Science (PLoS)</source><creator>Suo, Haicui ; Ma, Qibin ; Ye, Kaixin ; Yang, Cunyi ; Tang, Yujuan ; Hao, Juan ; Zhang, Zhanyuan J ; Chen, Mingluan ; Feng, Yuqi ; Nian, Hai</creator><contributor>Blazquez, Miguel A.</contributor><creatorcontrib>Suo, Haicui ; Ma, Qibin ; Ye, Kaixin ; Yang, Cunyi ; Tang, Yujuan ; Hao, Juan ; Zhang, Zhanyuan J ; Chen, Mingluan ; Feng, Yuqi ; Nian, Hai ; Blazquez, Miguel A.</creatorcontrib><description>Gibberellic acids (GAs) are plant hormones that play fundamental roles in plant growth and developmental processes. Previous studies have demonstrated that three key enzymes of GA20ox, GA3ox, and GA2ox are involved in GA biosynthesis. In this study, the Arabidopsis DREB1A gene driven by the CaMV 35S promoter was introduced into soybean plants by Agrobacterium- mediated transformation. The results showed that the transgenic soybean plants exhibited a typical phenotype of GA-deficient mutants, such as severe dwarfism, small and dark-green leaves, and late flowering compared to those of the non-transgenic plants. The dwarfism phenotype was rescued by the application of exogenous GA(3) once a week for three weeks with the concentrations of 144 µM or three times in one week with the concentrations of 60 µM. Quantitative RT-PCR analysis revealed that the transcription levels of the GA synthase genes were higher in the transgenic soybean plants than those in controls, whereas GA-deactivated genes except GmGA2ox4 showed lower levels of expression. The transcript level of GmGA2ox4 encoding the only deactivation enzyme using C(20)-GAs as the substrates in soybean was dramatically enhanced in transgenic plants compared to that of wide type. Furthermore, the contents of endogenous bioactive GAs were significantly decreased in transgenic plants than those of wide type. The results suggested that AtDREB1A could cause dwarfism mediated by GA biosynthesis pathway in soybean.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0045568</identifier><identifier>PMID: 23029105</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Analysis ; Arabidopsis ; Arabidopsis Proteins - genetics ; Arabidopsis thaliana ; Biology ; Biosynthesis ; Cloning ; Deactivation ; Deficient mutant ; Dwarfism ; Dwarfism - genetics ; Flowering ; Gene Expression ; Genes ; Genetic aspects ; Genetic transformation ; Gibberellins - metabolism ; Gibberellins - pharmacology ; Glycine max ; Glycine max - anatomy & histology ; Glycine max - drug effects ; Glycine max - genetics ; Glycine max - metabolism ; Hormones ; Laboratories ; Leaves ; Mutants ; Oryza ; Phenotype ; Physiological aspects ; Plant growth ; Plant hormones ; Plant Leaves - anatomy & histology ; Plant Leaves - drug effects ; Plants (botany) ; Plants, Genetically Modified ; Polymerase chain reaction ; Proteins ; Salinity ; Seeds - anatomy & histology ; Seeds - drug effects ; Soybeans ; Substrates ; Tobacco ; Transcription ; Transcription factors ; Transcription Factors - genetics ; Transformation ; Transgenic plants</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e45568-e45568</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Suo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Suo et al 2012 Suo et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c659t-a8cc0328d168e4c5299ed70233df02f97ce08c7b7d2a05a579d44742d21385c03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445508/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445508/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23029105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Blazquez, Miguel A.</contributor><creatorcontrib>Suo, Haicui</creatorcontrib><creatorcontrib>Ma, Qibin</creatorcontrib><creatorcontrib>Ye, Kaixin</creatorcontrib><creatorcontrib>Yang, Cunyi</creatorcontrib><creatorcontrib>Tang, Yujuan</creatorcontrib><creatorcontrib>Hao, Juan</creatorcontrib><creatorcontrib>Zhang, Zhanyuan J</creatorcontrib><creatorcontrib>Chen, Mingluan</creatorcontrib><creatorcontrib>Feng, Yuqi</creatorcontrib><creatorcontrib>Nian, Hai</creatorcontrib><title>Overexpression of AtDREB1A causes a severe dwarf phenotype by decreasing endogenous gibberellin levels in soybean [Glycine max (L.) Merr]</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Gibberellic acids (GAs) are plant hormones that play fundamental roles in plant growth and developmental processes. Previous studies have demonstrated that three key enzymes of GA20ox, GA3ox, and GA2ox are involved in GA biosynthesis. In this study, the Arabidopsis DREB1A gene driven by the CaMV 35S promoter was introduced into soybean plants by Agrobacterium- mediated transformation. The results showed that the transgenic soybean plants exhibited a typical phenotype of GA-deficient mutants, such as severe dwarfism, small and dark-green leaves, and late flowering compared to those of the non-transgenic plants. The dwarfism phenotype was rescued by the application of exogenous GA(3) once a week for three weeks with the concentrations of 144 µM or three times in one week with the concentrations of 60 µM. Quantitative RT-PCR analysis revealed that the transcription levels of the GA synthase genes were higher in the transgenic soybean plants than those in controls, whereas GA-deactivated genes except GmGA2ox4 showed lower levels of expression. The transcript level of GmGA2ox4 encoding the only deactivation enzyme using C(20)-GAs as the substrates in soybean was dramatically enhanced in transgenic plants compared to that of wide type. Furthermore, the contents of endogenous bioactive GAs were significantly decreased in transgenic plants than those of wide type. The results suggested that AtDREB1A could cause dwarfism mediated by GA biosynthesis pathway in soybean.</description><subject>Agriculture</subject><subject>Analysis</subject><subject>Arabidopsis</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Biology</subject><subject>Biosynthesis</subject><subject>Cloning</subject><subject>Deactivation</subject><subject>Deficient mutant</subject><subject>Dwarfism</subject><subject>Dwarfism - genetics</subject><subject>Flowering</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genetic transformation</subject><subject>Gibberellins - metabolism</subject><subject>Gibberellins - pharmacology</subject><subject>Glycine max</subject><subject>Glycine max - anatomy & histology</subject><subject>Glycine max - drug effects</subject><subject>Glycine max - genetics</subject><subject>Glycine max - metabolism</subject><subject>Hormones</subject><subject>Laboratories</subject><subject>Leaves</subject><subject>Mutants</subject><subject>Oryza</subject><subject>Phenotype</subject><subject>Physiological aspects</subject><subject>Plant growth</subject><subject>Plant hormones</subject><subject>Plant Leaves - anatomy & histology</subject><subject>Plant Leaves - drug effects</subject><subject>Plants (botany)</subject><subject>Plants, Genetically Modified</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Salinity</subject><subject>Seeds - anatomy & histology</subject><subject>Seeds - drug effects</subject><subject>Soybeans</subject><subject>Substrates</subject><subject>Tobacco</subject><subject>Transcription</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transformation</subject><subject>Transgenic plants</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUl1rFDEUHUSxdfUfiAZ8qQ-75mu-XoS11lpYKYg-iYRMcjPNMpuMyWzt_gT_tRl3WrpS8pBL7jkn9x5Olr0keEFYSd6t_TY42S1672CBMc_zonqUHZOa0XlBMXt8rz7KnsW4xjhnVVE8zY4ow7QmOD_O_lxeQ4CbPkCM1jvkDVoOH7-efSBLpOQ2QkQSRRhBSP-WwaD-Cpwfdj2gZoc0qAAyWtcicNq3qbWNqLVNkwhdZx3qEreLKFXR7xqQDv0473bKOkAbeYNOVou36AuE8PN59sTILsKL6Z5l3z-dfTv9PF9dnl-cLldzVeT1MJeVUpjRSpOiAq5yWtegS0wZ0wZTU5cKcKXKptRU4lzmZa05LznVlLAqT9RZ9nqv23c-isnFKAjjPMcVo3VCXOwR2su16IPdyLATXlrx78GHVsgwWNWBIE3BOEs-11XJDSslNSYJFUyauuAcktb76bdtswGtwA1Bdgeihx1nr0Trr8U4zjjPLDuZBIL_tYU4iI2NKnkrHSSvBcEV5bjmpEjQN_9BH95uQrUyLWCd8elfNYqKJa8rWiS5UWvxACodDRurUuSMTe8HBL4nqOBjDGDudiRYjIG9HUaMgRVTYBPt1X1_7ki3CWV_AZOG51Y</recordid><startdate>20120918</startdate><enddate>20120918</enddate><creator>Suo, Haicui</creator><creator>Ma, Qibin</creator><creator>Ye, Kaixin</creator><creator>Yang, Cunyi</creator><creator>Tang, Yujuan</creator><creator>Hao, Juan</creator><creator>Zhang, Zhanyuan J</creator><creator>Chen, Mingluan</creator><creator>Feng, Yuqi</creator><creator>Nian, Hai</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120918</creationdate><title>Overexpression of AtDREB1A causes a severe dwarf phenotype by decreasing endogenous gibberellin levels in soybean [Glycine max (L.) Merr]</title><author>Suo, Haicui ; Ma, Qibin ; Ye, Kaixin ; Yang, Cunyi ; Tang, Yujuan ; Hao, Juan ; Zhang, Zhanyuan J ; Chen, Mingluan ; Feng, Yuqi ; Nian, Hai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c659t-a8cc0328d168e4c5299ed70233df02f97ce08c7b7d2a05a579d44742d21385c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agriculture</topic><topic>Analysis</topic><topic>Arabidopsis</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis thaliana</topic><topic>Biology</topic><topic>Biosynthesis</topic><topic>Cloning</topic><topic>Deactivation</topic><topic>Deficient mutant</topic><topic>Dwarfism</topic><topic>Dwarfism - genetics</topic><topic>Flowering</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genetic transformation</topic><topic>Gibberellins - metabolism</topic><topic>Gibberellins - pharmacology</topic><topic>Glycine max</topic><topic>Glycine max - anatomy & histology</topic><topic>Glycine max - drug effects</topic><topic>Glycine max - genetics</topic><topic>Glycine max - metabolism</topic><topic>Hormones</topic><topic>Laboratories</topic><topic>Leaves</topic><topic>Mutants</topic><topic>Oryza</topic><topic>Phenotype</topic><topic>Physiological aspects</topic><topic>Plant growth</topic><topic>Plant hormones</topic><topic>Plant Leaves - anatomy & histology</topic><topic>Plant Leaves - drug effects</topic><topic>Plants (botany)</topic><topic>Plants, Genetically Modified</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Salinity</topic><topic>Seeds - anatomy & histology</topic><topic>Seeds - drug effects</topic><topic>Soybeans</topic><topic>Substrates</topic><topic>Tobacco</topic><topic>Transcription</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transformation</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suo, Haicui</creatorcontrib><creatorcontrib>Ma, Qibin</creatorcontrib><creatorcontrib>Ye, Kaixin</creatorcontrib><creatorcontrib>Yang, Cunyi</creatorcontrib><creatorcontrib>Tang, Yujuan</creatorcontrib><creatorcontrib>Hao, Juan</creatorcontrib><creatorcontrib>Zhang, Zhanyuan J</creatorcontrib><creatorcontrib>Chen, Mingluan</creatorcontrib><creatorcontrib>Feng, Yuqi</creatorcontrib><creatorcontrib>Nian, Hai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suo, Haicui</au><au>Ma, Qibin</au><au>Ye, Kaixin</au><au>Yang, Cunyi</au><au>Tang, Yujuan</au><au>Hao, Juan</au><au>Zhang, Zhanyuan J</au><au>Chen, Mingluan</au><au>Feng, Yuqi</au><au>Nian, Hai</au><au>Blazquez, Miguel A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Overexpression of AtDREB1A causes a severe dwarf phenotype by decreasing endogenous gibberellin levels in soybean [Glycine max (L.) Merr]</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-09-18</date><risdate>2012</risdate><volume>7</volume><issue>9</issue><spage>e45568</spage><epage>e45568</epage><pages>e45568-e45568</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Gibberellic acids (GAs) are plant hormones that play fundamental roles in plant growth and developmental processes. Previous studies have demonstrated that three key enzymes of GA20ox, GA3ox, and GA2ox are involved in GA biosynthesis. In this study, the Arabidopsis DREB1A gene driven by the CaMV 35S promoter was introduced into soybean plants by Agrobacterium- mediated transformation. The results showed that the transgenic soybean plants exhibited a typical phenotype of GA-deficient mutants, such as severe dwarfism, small and dark-green leaves, and late flowering compared to those of the non-transgenic plants. The dwarfism phenotype was rescued by the application of exogenous GA(3) once a week for three weeks with the concentrations of 144 µM or three times in one week with the concentrations of 60 µM. Quantitative RT-PCR analysis revealed that the transcription levels of the GA synthase genes were higher in the transgenic soybean plants than those in controls, whereas GA-deactivated genes except GmGA2ox4 showed lower levels of expression. The transcript level of GmGA2ox4 encoding the only deactivation enzyme using C(20)-GAs as the substrates in soybean was dramatically enhanced in transgenic plants compared to that of wide type. Furthermore, the contents of endogenous bioactive GAs were significantly decreased in transgenic plants than those of wide type. The results suggested that AtDREB1A could cause dwarfism mediated by GA biosynthesis pathway in soybean.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23029105</pmid><doi>10.1371/journal.pone.0045568</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Agriculture Analysis Arabidopsis Arabidopsis Proteins - genetics Arabidopsis thaliana Biology Biosynthesis Cloning Deactivation Deficient mutant Dwarfism Dwarfism - genetics Flowering Gene Expression Genes Genetic aspects Genetic transformation Gibberellins - metabolism Gibberellins - pharmacology Glycine max Glycine max - anatomy & histology Glycine max - drug effects Glycine max - genetics Glycine max - metabolism Hormones Laboratories Leaves Mutants Oryza Phenotype Physiological aspects Plant growth Plant hormones Plant Leaves - anatomy & histology Plant Leaves - drug effects Plants (botany) Plants, Genetically Modified Polymerase chain reaction Proteins Salinity Seeds - anatomy & histology Seeds - drug effects Soybeans Substrates Tobacco Transcription Transcription factors Transcription Factors - genetics Transformation Transgenic plants |
| title | Overexpression of AtDREB1A causes a severe dwarf phenotype by decreasing endogenous gibberellin levels in soybean [Glycine max (L.) Merr] |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T18%3A19%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Overexpression%20of%20AtDREB1A%20causes%20a%20severe%20dwarf%20phenotype%20by%20decreasing%20endogenous%20gibberellin%20levels%20in%20soybean%20%5BGlycine%20max%20(L.)%20Merr%5D&rft.jtitle=PloS%20one&rft.au=Suo,%20Haicui&rft.date=2012-09-18&rft.volume=7&rft.issue=9&rft.spage=e45568&rft.epage=e45568&rft.pages=e45568-e45568&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0045568&rft_dat=%3Cgale_plos_%3EA498262406%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1344508329&rft_id=info:pmid/23029105&rft_galeid=A498262406&rft_doaj_id=oai_doaj_org_article_1b63431939874f37a2ff34463af9644e&rfr_iscdi=true |