Involvement of microRNA-mediated gene expression regulation in the pathological development of stem canker disease in Populus trichocarpa
MicroRNAs (miRNAs), a type of short (21-23 nucleotides), non-coding RNA molecule, mediate repressive gene regulation through RNA silencing at the post-transcriptional level, and play an important role in defense and response to abiotic and biotic stresses. In the present study, Affymetrix® miRNA Arr...
Gespeichert in:
| Veröffentlicht in: | PloS one 2012-09, Vol.7 (9), p.e44968-e44968 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e44968 |
|---|---|
| container_issue | 9 |
| container_start_page | e44968 |
| container_title | PloS one |
| container_volume | 7 |
| creator | Zhao, Jia-Ping Jiang, Xiao-Ling Zhang, Bing-Yu Su, Xiao-Hua |
| description | MicroRNAs (miRNAs), a type of short (21-23 nucleotides), non-coding RNA molecule, mediate repressive gene regulation through RNA silencing at the post-transcriptional level, and play an important role in defense and response to abiotic and biotic stresses. In the present study, Affymetrix® miRNA Array, real-time quantitative PCR (qPCR) for miRNAs and their targets, and miRNA promoter analysis were used to validate the gene expression patterns of miRNAs in Populus trichocarpa plantlets induced with the poplar stem canker pathogen, Botryosphaeria dothidea. Twelve miRNAs (miR156, miR159, miR160, miR164, miR166, miR168, miR172, miR319, miR398, miR408, miR1448, and miR1450) were upregulated in the stem bark of P. trichocarpa, but no downregulated miRNAs were found. Based on analysis of the miRNAs and their targets, a potential co-regulatory network was developed to describe post-transcriptional regulation in the pathological development of poplar stem canker. There was highly complex cross-talk between diverse miRNA pathway responses to biotic and abiotic stresses. The results suggest that miR156 is probably an integral component of the miRNA response to all environmental stresses in plants. Cis-regulatory elements were binding sites for the transcription factors (TFs) on DNA. Promoter analysis revealed that TC-rich repeats and a W1-box motif were both tightly related disease response motifs in Populus. Promoter analysis and target analysis of miRNAs also revealed that some TFs regulate their activation/repression. Furthermore, a feedback regulatory network in the pathological development of poplar stem canker is provided. The results confirm that miRNA pathways regulate gene expression during the pathological development of plant disease, and provide new insights into understanding the onset and development of poplar stem canker. |
| doi_str_mv | 10.1371/journal.pone.0044968 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1344508259</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A498262452</galeid><doaj_id>oai_doaj_org_article_9c3ceb04e854432686e3fec5d73e0302</doaj_id><sourcerecordid>A498262452</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-b3ff2dafe3164a6cb4c6a96b48767f61deaa3f0d881059b604e41ee48ddf44f33</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7rr6D0QLgujFjEmTZtobYVj8GFhcWT9uQ5qetFkzTU3SYf0J_mtTp7NMZS8kFw3pc95z8uacJHmK0RKTFX5zbQfXCbPsbQdLhCgtWXEvOcUlyRYsQ-T-0f4keeT9NUI5KRh7mJxkBGXFCpWnye9Nt7NmB1voQmpVutXS2atP68UWai0C1GkDHaRw0zvwXtsuddAMRoRxq7s0tJD2IrTW2EZLYdIadmBsf9DzAbapFN0PcGmtPQgPY9hn2w9m8GlwWrZWCteLx8kDJYyHJ9P3LPn2_t3X84-Li8sPm_P1xUKyMguLiiiV1UIBwYwKJisqmShZRYsVWymGaxCCKFQXBUZ5WTFEgWIAWtS1olQRcpY83-v2xno-ueg5JpTmqMjyMhKbPVFbcc17p7fC_eJWaP73wLqGCxe0NMBLSSRUMUeRU0oyVjAgCmRerwigaHLUejtlG6poqYy2OGFmovM_nW55Y3d8LIfhIgq8mgSc_TmAD3yrvQRjRAd2iHXHmiliOBvrfvEPevftJqoR8QK6UzbmlaMoX9OyyFhG87Hu5R1UXDXEFoktp3Q8nwW8ngVEJsBNaMTgPd98ufp_9vL7nH15xLYgTGi9NcPYgH4O0j0YG9h7B-rWZIz4ODEHN_g4MXyamBj27PiBboMOI0L-AHE_E8U</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1344508259</pqid></control><display><type>article</type><title>Involvement of microRNA-mediated gene expression regulation in the pathological development of stem canker disease in Populus trichocarpa</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><source>EZB Electronic Journals Library</source><creator>Zhao, Jia-Ping ; Jiang, Xiao-Ling ; Zhang, Bing-Yu ; Su, Xiao-Hua</creator><creatorcontrib>Zhao, Jia-Ping ; Jiang, Xiao-Ling ; Zhang, Bing-Yu ; Su, Xiao-Hua</creatorcontrib><description>MicroRNAs (miRNAs), a type of short (21-23 nucleotides), non-coding RNA molecule, mediate repressive gene regulation through RNA silencing at the post-transcriptional level, and play an important role in defense and response to abiotic and biotic stresses. In the present study, Affymetrix® miRNA Array, real-time quantitative PCR (qPCR) for miRNAs and their targets, and miRNA promoter analysis were used to validate the gene expression patterns of miRNAs in Populus trichocarpa plantlets induced with the poplar stem canker pathogen, Botryosphaeria dothidea. Twelve miRNAs (miR156, miR159, miR160, miR164, miR166, miR168, miR172, miR319, miR398, miR408, miR1448, and miR1450) were upregulated in the stem bark of P. trichocarpa, but no downregulated miRNAs were found. Based on analysis of the miRNAs and their targets, a potential co-regulatory network was developed to describe post-transcriptional regulation in the pathological development of poplar stem canker. There was highly complex cross-talk between diverse miRNA pathway responses to biotic and abiotic stresses. The results suggest that miR156 is probably an integral component of the miRNA response to all environmental stresses in plants. Cis-regulatory elements were binding sites for the transcription factors (TFs) on DNA. Promoter analysis revealed that TC-rich repeats and a W1-box motif were both tightly related disease response motifs in Populus. Promoter analysis and target analysis of miRNAs also revealed that some TFs regulate their activation/repression. Furthermore, a feedback regulatory network in the pathological development of poplar stem canker is provided. The results confirm that miRNA pathways regulate gene expression during the pathological development of plant disease, and provide new insights into understanding the onset and development of poplar stem canker.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0044968</identifier><identifier>PMID: 23028709</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abiotic stress ; Agriculture ; Analysis ; Arabidopsis ; Ascomycota - physiology ; Bark ; Binding sites ; Bioinformatics ; Biology ; Botryosphaeria ; Deoxyribonucleic acid ; DNA ; Environmental stress ; Forestry ; Gene expression ; Gene Expression Regulation, Plant ; Gene regulation ; Gene silencing ; Genes, Plant - genetics ; Genetic aspects ; Genetic regulation ; Genetics ; Infections ; Laboratories ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Molecular biology ; Non-coding RNA ; Nucleotides ; Oryza ; Physiological aspects ; Pinus taeda ; Plant diseases ; Plant Diseases - microbiology ; Plantlets ; Plants (botany) ; Poplar ; Populus ; Populus - genetics ; Populus - microbiology ; Populus - physiology ; Post-transcription ; Promoter Regions, Genetic - genetics ; Proteins ; Regulatory sequences ; Ribonucleic acid ; RNA ; RNA, Plant - genetics ; RNA, Plant - metabolism ; RNA-mediated interference ; Stem canker ; Stems ; Stress, Physiological ; Stresses ; Studies ; Symbiosis ; Transcription factors ; Up-Regulation</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e44968-e44968</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Zhao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Zhao et al 2012 Zhao et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-b3ff2dafe3164a6cb4c6a96b48767f61deaa3f0d881059b604e41ee48ddf44f33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445618/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3445618/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23028709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Jia-Ping</creatorcontrib><creatorcontrib>Jiang, Xiao-Ling</creatorcontrib><creatorcontrib>Zhang, Bing-Yu</creatorcontrib><creatorcontrib>Su, Xiao-Hua</creatorcontrib><title>Involvement of microRNA-mediated gene expression regulation in the pathological development of stem canker disease in Populus trichocarpa</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>MicroRNAs (miRNAs), a type of short (21-23 nucleotides), non-coding RNA molecule, mediate repressive gene regulation through RNA silencing at the post-transcriptional level, and play an important role in defense and response to abiotic and biotic stresses. In the present study, Affymetrix® miRNA Array, real-time quantitative PCR (qPCR) for miRNAs and their targets, and miRNA promoter analysis were used to validate the gene expression patterns of miRNAs in Populus trichocarpa plantlets induced with the poplar stem canker pathogen, Botryosphaeria dothidea. Twelve miRNAs (miR156, miR159, miR160, miR164, miR166, miR168, miR172, miR319, miR398, miR408, miR1448, and miR1450) were upregulated in the stem bark of P. trichocarpa, but no downregulated miRNAs were found. Based on analysis of the miRNAs and their targets, a potential co-regulatory network was developed to describe post-transcriptional regulation in the pathological development of poplar stem canker. There was highly complex cross-talk between diverse miRNA pathway responses to biotic and abiotic stresses. The results suggest that miR156 is probably an integral component of the miRNA response to all environmental stresses in plants. Cis-regulatory elements were binding sites for the transcription factors (TFs) on DNA. Promoter analysis revealed that TC-rich repeats and a W1-box motif were both tightly related disease response motifs in Populus. Promoter analysis and target analysis of miRNAs also revealed that some TFs regulate their activation/repression. Furthermore, a feedback regulatory network in the pathological development of poplar stem canker is provided. The results confirm that miRNA pathways regulate gene expression during the pathological development of plant disease, and provide new insights into understanding the onset and development of poplar stem canker.</description><subject>Abiotic stress</subject><subject>Agriculture</subject><subject>Analysis</subject><subject>Arabidopsis</subject><subject>Ascomycota - physiology</subject><subject>Bark</subject><subject>Binding sites</subject><subject>Bioinformatics</subject><subject>Biology</subject><subject>Botryosphaeria</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Environmental stress</subject><subject>Forestry</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene regulation</subject><subject>Gene silencing</subject><subject>Genes, Plant - genetics</subject><subject>Genetic aspects</subject><subject>Genetic regulation</subject><subject>Genetics</subject><subject>Infections</subject><subject>Laboratories</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Molecular biology</subject><subject>Non-coding RNA</subject><subject>Nucleotides</subject><subject>Oryza</subject><subject>Physiological aspects</subject><subject>Pinus taeda</subject><subject>Plant diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plantlets</subject><subject>Plants (botany)</subject><subject>Poplar</subject><subject>Populus</subject><subject>Populus - genetics</subject><subject>Populus - microbiology</subject><subject>Populus - physiology</subject><subject>Post-transcription</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Proteins</subject><subject>Regulatory sequences</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Plant - genetics</subject><subject>RNA, Plant - metabolism</subject><subject>RNA-mediated interference</subject><subject>Stem canker</subject><subject>Stems</subject><subject>Stress, Physiological</subject><subject>Stresses</subject><subject>Studies</subject><subject>Symbiosis</subject><subject>Transcription factors</subject><subject>Up-Regulation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjEmTZtobYVj8GFhcWT9uQ5qetFkzTU3SYf0J_mtTp7NMZS8kFw3pc95z8uacJHmK0RKTFX5zbQfXCbPsbQdLhCgtWXEvOcUlyRYsQ-T-0f4keeT9NUI5KRh7mJxkBGXFCpWnye9Nt7NmB1voQmpVutXS2atP68UWai0C1GkDHaRw0zvwXtsuddAMRoRxq7s0tJD2IrTW2EZLYdIadmBsf9DzAbapFN0PcGmtPQgPY9hn2w9m8GlwWrZWCteLx8kDJYyHJ9P3LPn2_t3X84-Li8sPm_P1xUKyMguLiiiV1UIBwYwKJisqmShZRYsVWymGaxCCKFQXBUZ5WTFEgWIAWtS1olQRcpY83-v2xno-ueg5JpTmqMjyMhKbPVFbcc17p7fC_eJWaP73wLqGCxe0NMBLSSRUMUeRU0oyVjAgCmRerwigaHLUejtlG6poqYy2OGFmovM_nW55Y3d8LIfhIgq8mgSc_TmAD3yrvQRjRAd2iHXHmiliOBvrfvEPevftJqoR8QK6UzbmlaMoX9OyyFhG87Hu5R1UXDXEFoktp3Q8nwW8ngVEJsBNaMTgPd98ufp_9vL7nH15xLYgTGi9NcPYgH4O0j0YG9h7B-rWZIz4ODEHN_g4MXyamBj27PiBboMOI0L-AHE_E8U</recordid><startdate>20120918</startdate><enddate>20120918</enddate><creator>Zhao, Jia-Ping</creator><creator>Jiang, Xiao-Ling</creator><creator>Zhang, Bing-Yu</creator><creator>Su, Xiao-Hua</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120918</creationdate><title>Involvement of microRNA-mediated gene expression regulation in the pathological development of stem canker disease in Populus trichocarpa</title><author>Zhao, Jia-Ping ; Jiang, Xiao-Ling ; Zhang, Bing-Yu ; Su, Xiao-Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-b3ff2dafe3164a6cb4c6a96b48767f61deaa3f0d881059b604e41ee48ddf44f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Abiotic stress</topic><topic>Agriculture</topic><topic>Analysis</topic><topic>Arabidopsis</topic><topic>Ascomycota - physiology</topic><topic>Bark</topic><topic>Binding sites</topic><topic>Bioinformatics</topic><topic>Biology</topic><topic>Botryosphaeria</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Environmental stress</topic><topic>Forestry</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene regulation</topic><topic>Gene silencing</topic><topic>Genes, Plant - genetics</topic><topic>Genetic aspects</topic><topic>Genetic regulation</topic><topic>Genetics</topic><topic>Infections</topic><topic>Laboratories</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Molecular biology</topic><topic>Non-coding RNA</topic><topic>Nucleotides</topic><topic>Oryza</topic><topic>Physiological aspects</topic><topic>Pinus taeda</topic><topic>Plant diseases</topic><topic>Plant Diseases - microbiology</topic><topic>Plantlets</topic><topic>Plants (botany)</topic><topic>Poplar</topic><topic>Populus</topic><topic>Populus - genetics</topic><topic>Populus - microbiology</topic><topic>Populus - physiology</topic><topic>Post-transcription</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Proteins</topic><topic>Regulatory sequences</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Plant - genetics</topic><topic>RNA, Plant - metabolism</topic><topic>RNA-mediated interference</topic><topic>Stem canker</topic><topic>Stems</topic><topic>Stress, Physiological</topic><topic>Stresses</topic><topic>Studies</topic><topic>Symbiosis</topic><topic>Transcription factors</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Jia-Ping</creatorcontrib><creatorcontrib>Jiang, Xiao-Ling</creatorcontrib><creatorcontrib>Zhang, Bing-Yu</creatorcontrib><creatorcontrib>Su, Xiao-Hua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints Resource Center</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>https://resources.nclive.org/materials</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Jia-Ping</au><au>Jiang, Xiao-Ling</au><au>Zhang, Bing-Yu</au><au>Su, Xiao-Hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of microRNA-mediated gene expression regulation in the pathological development of stem canker disease in Populus trichocarpa</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-09-18</date><risdate>2012</risdate><volume>7</volume><issue>9</issue><spage>e44968</spage><epage>e44968</epage><pages>e44968-e44968</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>MicroRNAs (miRNAs), a type of short (21-23 nucleotides), non-coding RNA molecule, mediate repressive gene regulation through RNA silencing at the post-transcriptional level, and play an important role in defense and response to abiotic and biotic stresses. In the present study, Affymetrix® miRNA Array, real-time quantitative PCR (qPCR) for miRNAs and their targets, and miRNA promoter analysis were used to validate the gene expression patterns of miRNAs in Populus trichocarpa plantlets induced with the poplar stem canker pathogen, Botryosphaeria dothidea. Twelve miRNAs (miR156, miR159, miR160, miR164, miR166, miR168, miR172, miR319, miR398, miR408, miR1448, and miR1450) were upregulated in the stem bark of P. trichocarpa, but no downregulated miRNAs were found. Based on analysis of the miRNAs and their targets, a potential co-regulatory network was developed to describe post-transcriptional regulation in the pathological development of poplar stem canker. There was highly complex cross-talk between diverse miRNA pathway responses to biotic and abiotic stresses. The results suggest that miR156 is probably an integral component of the miRNA response to all environmental stresses in plants. Cis-regulatory elements were binding sites for the transcription factors (TFs) on DNA. Promoter analysis revealed that TC-rich repeats and a W1-box motif were both tightly related disease response motifs in Populus. Promoter analysis and target analysis of miRNAs also revealed that some TFs regulate their activation/repression. Furthermore, a feedback regulatory network in the pathological development of poplar stem canker is provided. The results confirm that miRNA pathways regulate gene expression during the pathological development of plant disease, and provide new insights into understanding the onset and development of poplar stem canker.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23028709</pmid><doi>10.1371/journal.pone.0044968</doi><tpages>e44968</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-09, Vol.7 (9), p.e44968-e44968 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1344508259 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Abiotic stress Agriculture Analysis Arabidopsis Ascomycota - physiology Bark Binding sites Bioinformatics Biology Botryosphaeria Deoxyribonucleic acid DNA Environmental stress Forestry Gene expression Gene Expression Regulation, Plant Gene regulation Gene silencing Genes, Plant - genetics Genetic aspects Genetic regulation Genetics Infections Laboratories MicroRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Molecular biology Non-coding RNA Nucleotides Oryza Physiological aspects Pinus taeda Plant diseases Plant Diseases - microbiology Plantlets Plants (botany) Poplar Populus Populus - genetics Populus - microbiology Populus - physiology Post-transcription Promoter Regions, Genetic - genetics Proteins Regulatory sequences Ribonucleic acid RNA RNA, Plant - genetics RNA, Plant - metabolism RNA-mediated interference Stem canker Stems Stress, Physiological Stresses Studies Symbiosis Transcription factors Up-Regulation |
| title | Involvement of microRNA-mediated gene expression regulation in the pathological development of stem canker disease in Populus trichocarpa |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T18%3A02%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Involvement%20of%20microRNA-mediated%20gene%20expression%20regulation%20in%20the%20pathological%20development%20of%20stem%20canker%20disease%20in%20Populus%20trichocarpa&rft.jtitle=PloS%20one&rft.au=Zhao,%20Jia-Ping&rft.date=2012-09-18&rft.volume=7&rft.issue=9&rft.spage=e44968&rft.epage=e44968&rft.pages=e44968-e44968&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0044968&rft_dat=%3Cgale_plos_%3EA498262452%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1344508259&rft_id=info:pmid/23028709&rft_galeid=A498262452&rft_doaj_id=oai_doaj_org_article_9c3ceb04e854432686e3fec5d73e0302&rfr_iscdi=true |