A portable chemotaxis platform for short and long term analysis
Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps...
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| Veröffentlicht in: | PloS one 2012-09, Vol.7 (9), p.e44995-e44995 |
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| creator | Xu, Chenjie Poh, Yuk Kee C Roes, Isaac O'Cearbhaill, Eoin D Matthiesen, Mads Emil Mu, Luye Yang, Seung Yun Miranda-Nieves, David Irimia, Daniel Karp, Jeffrey M |
| description | Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZET® osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate and can generate well-defined, stable chemical gradients continuously for up to 7 days. Using this platform, we validated the short-term (24 hours) and long-term (72 hours) concentration dependent PDGF-BB chemotaxis response of human bone marrow derived mesenchymal stem cells. |
| doi_str_mv | 10.1371/journal.pone.0044995 |
| format | Article |
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Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZET® osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate and can generate well-defined, stable chemical gradients continuously for up to 7 days. Using this platform, we validated the short-term (24 hours) and long-term (72 hours) concentration dependent PDGF-BB chemotaxis response of human bone marrow derived mesenchymal stem cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0044995</identifier><identifier>PMID: 23028729</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biology ; Biomedical engineering ; Bone marrow ; Cell adhesion & migration ; Cell culture ; Cell migration ; Cell Migration Assays ; Chemotaxis ; Engineering ; Generators ; Genetic aspects ; Health sciences ; Hospitals ; Human behavior ; Humans ; Medical schools ; Medicine ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; Mesenchyme ; Microfluidics ; Microfluidics - instrumentation ; Microfluidics - methods ; Osmosis ; Physiological aspects ; Platelet-derived growth factor ; Platelet-derived growth factor BB ; Pumps ; Shear stress ; Stem cells ; Studies ; Time Factors ; Womens health</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e44995-e44995</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Xu et al. 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Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Xu et al 2012 Xu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-345d03d4833639a609ad97dbc3d129164737b270293c60815570a4e3257ff4503</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444504/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3444504/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23028729$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Rao, Jianghong</contributor><creatorcontrib>Xu, Chenjie</creatorcontrib><creatorcontrib>Poh, Yuk Kee C</creatorcontrib><creatorcontrib>Roes, Isaac</creatorcontrib><creatorcontrib>O'Cearbhaill, Eoin D</creatorcontrib><creatorcontrib>Matthiesen, Mads Emil</creatorcontrib><creatorcontrib>Mu, Luye</creatorcontrib><creatorcontrib>Yang, Seung Yun</creatorcontrib><creatorcontrib>Miranda-Nieves, David</creatorcontrib><creatorcontrib>Irimia, Daniel</creatorcontrib><creatorcontrib>Karp, Jeffrey M</creatorcontrib><title>A portable chemotaxis platform for short and long term analysis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Flow-based microfluidic systems have been widely utilized for cell migration studies given their ability to generate versatile and precisely defined chemical gradients and to permit direct visualization of migrating cells. 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Nonetheless, the general need for bulky peripherals such as mechanical pumps and tubing and the complicated setup procedures significantly limit the widespread use of these microfluidic systems for cell migration studies. Here we present a simple method to power microfluidic devices for chemotaxis assays using the commercially available ALZET® osmotic pumps. Specifically, we developed a standalone chemotaxis platform that has the same footprint as a multiwell plate and can generate well-defined, stable chemical gradients continuously for up to 7 days. Using this platform, we validated the short-term (24 hours) and long-term (72 hours) concentration dependent PDGF-BB chemotaxis response of human bone marrow derived mesenchymal stem cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23028729</pmid><doi>10.1371/journal.pone.0044995</doi><tpages>e44995</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biology Biomedical engineering Bone marrow Cell adhesion & migration Cell culture Cell migration Cell Migration Assays Chemotaxis Engineering Generators Genetic aspects Health sciences Hospitals Human behavior Humans Medical schools Medicine Mesenchymal stem cells Mesenchymal Stem Cells - cytology Mesenchyme Microfluidics Microfluidics - instrumentation Microfluidics - methods Osmosis Physiological aspects Platelet-derived growth factor Platelet-derived growth factor BB Pumps Shear stress Stem cells Studies Time Factors Womens health |
| title | A portable chemotaxis platform for short and long term analysis |
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