Defining the genomic signature of totipotency and pluripotency during early human development
The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (I...
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| Veröffentlicht in: | PloS one 2013-04, Vol.8 (4), p.e62135-e62135 |
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| creator | Galan, Amparo Diaz-Gimeno, Patricia Poo, Maria Eugenia Valbuena, Diana Sanchez, Eva Ruiz, Veronica Dopazo, Joaquin Montaner, David Conesa, Ana Simon, Carlos |
| description | The genetic mechanisms governing human pre-implantation embryo development and the in vitro counterparts, human embryonic stem cells (hESCs), still remain incomplete. Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation network signature (UNS) and applied it to a differential gene expression profile between single blastomeres from day-3 embryos, ICMs and hESCs. This allowed us to establish a unique signature composed of highly interconnected genes characteristic of totipotency (61 genes), in vivo pluripotency (20 genes), and in vitro pluripotency (107 genes), and which are also proprietary according to functional analysis. This systems biology approach has led to an improved understanding of the molecular and signaling processes governing human pre-implantation embryo development, as well as enabling us to comprehend how hESCs might adapt to in vitro culture conditions. |
| doi_str_mv | 10.1371/journal.pone.0062135 |
| format | Article |
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Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation network signature (UNS) and applied it to a differential gene expression profile between single blastomeres from day-3 embryos, ICMs and hESCs. This allowed us to establish a unique signature composed of highly interconnected genes characteristic of totipotency (61 genes), in vivo pluripotency (20 genes), and in vitro pluripotency (107 genes), and which are also proprietary according to functional analysis. This systems biology approach has led to an improved understanding of the molecular and signaling processes governing human pre-implantation embryo development, as well as enabling us to comprehend how hESCs might adapt to in vitro culture conditions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0062135</identifier><identifier>PMID: 23614026</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Bioinformatics ; Biology ; Blastocyst Inner Cell Mass - cytology ; Blastocyst Inner Cell Mass - metabolism ; Blastocysts ; Blastomeres ; Blastomeres - cytology ; Blastomeres - metabolism ; Cell culture ; Cell Differentiation - genetics ; Comparative analysis ; Deoxyribonucleic acid ; DNA ; DNA methylation ; Embryo cells ; Embryogenesis ; Embryonic development ; Embryonic Development - genetics ; Embryonic stem cells ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Embryos ; Functional analysis ; Gene expression ; Gene Expression Profiling ; Gene Regulatory Networks - genetics ; Genes ; Genome, Human - genetics ; Genomes ; Genomics ; Humans ; Implantation ; Molecular Sequence Annotation ; Ontology ; Pluripotency ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - metabolism ; Principal components analysis ; Signaling ; Stem cells ; Studies ; Surgical implants ; Totipotent Stem Cells - cytology ; Totipotent Stem Cells - metabolism</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e62135-e62135</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Galan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Previous global genome studies demonstrated that totipotent blastomeres from day-3 human embryos and pluripotent inner cell masses (ICMs) from blastocysts, display unique and differing transcriptomes. Nevertheless, comparative gene expression analysis has revealed that no significant differences exist between hESCs derived from blastomeres versus those obtained from ICMs, suggesting that pluripotent hESCs involve a new developmental progression. To understand early human stages evolution, we developed an undifferentiation network signature (UNS) and applied it to a differential gene expression profile between single blastomeres from day-3 embryos, ICMs and hESCs. This allowed us to establish a unique signature composed of highly interconnected genes characteristic of totipotency (61 genes), in vivo pluripotency (20 genes), and in vitro pluripotency (107 genes), and which are also proprietary according to functional analysis. This systems biology approach has led to an improved understanding of the molecular and signaling processes governing human pre-implantation embryo development, as well as enabling us to comprehend how hESCs might adapt to in vitro culture conditions.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23614026</pmid><doi>10.1371/journal.pone.0062135</doi><tpages>e62135</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-04, Vol.8 (4), p.e62135-e62135 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Bioinformatics Biology Blastocyst Inner Cell Mass - cytology Blastocyst Inner Cell Mass - metabolism Blastocysts Blastomeres Blastomeres - cytology Blastomeres - metabolism Cell culture Cell Differentiation - genetics Comparative analysis Deoxyribonucleic acid DNA DNA methylation Embryo cells Embryogenesis Embryonic development Embryonic Development - genetics Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Embryos Functional analysis Gene expression Gene Expression Profiling Gene Regulatory Networks - genetics Genes Genome, Human - genetics Genomes Genomics Humans Implantation Molecular Sequence Annotation Ontology Pluripotency Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Principal components analysis Signaling Stem cells Studies Surgical implants Totipotent Stem Cells - cytology Totipotent Stem Cells - metabolism |
| title | Defining the genomic signature of totipotency and pluripotency during early human development |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T05%3A00%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Defining%20the%20genomic%20signature%20of%20totipotency%20and%20pluripotency%20during%20early%20human%20development&rft.jtitle=PloS%20one&rft.au=Galan,%20Amparo&rft.date=2013-04-17&rft.volume=8&rft.issue=4&rft.spage=e62135&rft.epage=e62135&rft.pages=e62135-e62135&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0062135&rft_dat=%3Cgale_plos_%3EA478243096%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1344162532&rft_id=info:pmid/23614026&rft_galeid=A478243096&rft_doaj_id=oai_doaj_org_article_0d0e73180e0e49bc948a228200fa8b93&rfr_iscdi=true |