Parallelized TCSPC for dynamic intravital fluorescence lifetime imaging: quantifying neuronal dysfunction in neuroinflammation

Two-photon laser-scanning microscopy has revolutionized our view on vital processes by revealing motility and interaction patterns of various cell subsets in hardly accessible organs (e.g. brain) in living animals. However, current technology is still insufficient to elucidate the mechanisms of orga...

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Veröffentlicht in:	PloS one 2013-04, Vol.8 (4), p.e60100-e60100
Hauptverfasser:	Rinnenthal, Jan Leo, Börnchen, Christian, Radbruch, Helena, Andresen, Volker, Mossakowski, Agata, Siffrin, Volker, Seelemann, Thomas, Spiecker, Heinrich, Moll, Ingrid, Herz, Josephine, Hauser, Anja E, Zipp, Frauke, Behne, Martin J, Niesner, Raluca
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal tissues Animals Biology Biosensing Techniques - methods Biosensors Brain Brain - immunology Brain stem Calcium - metabolism Calibration Data acquisition Dermatology Detectors Diagnostic Imaging - methods Energy transfer Experimental allergic encephalomyelitis Fluorescence Fluorescence resonance energy transfer Fluorescence Resonance Energy Transfer - methods Health aspects Imaging In Vitro Techniques In vivo methods and tests Inflammation Innovations Lifetime Medical research Medicine Mice Microscopy Molecular modelling Multiple sclerosis Neuroimaging Neurology Neurons Neurophysiology New technology Organs Physics Pixels Proteins Rheumatism Scanning microscopy Scanning transmission electron microscopy Spinal cord Technology Time correlation functions
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