Neutrophil extracellular traps and bacterial biofilms in middle ear effusion of children with recurrent acute otitis media--a potential treatment target
Bacteria persist within biofilms on the middle ear mucosa of children with recurrent and chronic otitis media however the mechanisms by which these develop remain to be elucidated. Biopsies can be difficult to obtain from children and their small size limits analysis. In this study we aimed to inves...
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| Veröffentlicht in: | PloS one 2013-02, Vol.8 (2), p.e53837-e53837 |
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| creator | Thornton, Ruth B Wiertsema, Selma P Kirkham, Lea-Ann S Rigby, Paul J Vijayasekaran, Shyan Coates, Harvey L Richmond, Peter C |
| description | Bacteria persist within biofilms on the middle ear mucosa of children with recurrent and chronic otitis media however the mechanisms by which these develop remain to be elucidated. Biopsies can be difficult to obtain from children and their small size limits analysis.
In this study we aimed to investigate biofilm presence in middle ear effusion (MEE) from children with recurrent acute otitis media (rAOM) and to determine if these may represent infectious reservoirs similarly to those on the mucosa. We examined this through culture, viability staining and fluorescent in situ hybridisation (FISH) to determine bacterial species present. Most MEEs had live bacteria present using viability staining (32/36) and all effusions had bacteria present using the universal FISH probe (26/26). Of these, 70% contained 2 or more otopathogenic species. Extensive DNA stranding was also present. This DNA was largely host derived, representing neutrophil extracellular traps (NETs) within which live bacteria in biofilm formations were present. When treated with the recombinant human deoxyribonuclease 1, Dornase alfa, these strands were observed to fragment.
Bacterial biofilms, composed of multiple live otopathogenic species can be demonstrated in the MEEs of children with rAOM and that these contain extensive DNA stranding from NETs. The NETs contribute to the viscosity of the effusion, potentially contributing to its failure to clear as well as biofilm development. Our data indicates that Dornase alfa can fragment these strands and may play a role in future chronic OM treatment. |
| doi_str_mv | 10.1371/journal.pone.0053837 |
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In this study we aimed to investigate biofilm presence in middle ear effusion (MEE) from children with recurrent acute otitis media (rAOM) and to determine if these may represent infectious reservoirs similarly to those on the mucosa. We examined this through culture, viability staining and fluorescent in situ hybridisation (FISH) to determine bacterial species present. Most MEEs had live bacteria present using viability staining (32/36) and all effusions had bacteria present using the universal FISH probe (26/26). Of these, 70% contained 2 or more otopathogenic species. Extensive DNA stranding was also present. This DNA was largely host derived, representing neutrophil extracellular traps (NETs) within which live bacteria in biofilm formations were present. When treated with the recombinant human deoxyribonuclease 1, Dornase alfa, these strands were observed to fragment.
Bacterial biofilms, composed of multiple live otopathogenic species can be demonstrated in the MEEs of children with rAOM and that these contain extensive DNA stranding from NETs. The NETs contribute to the viscosity of the effusion, potentially contributing to its failure to clear as well as biofilm development. Our data indicates that Dornase alfa can fragment these strands and may play a role in future chronic OM treatment.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0053837</identifier><identifier>PMID: 23393551</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Bacteria ; Biofilms ; Biofilms - growth & development ; Biology ; Biopsy ; Children ; Children & youth ; Childrens health ; Cystic fibrosis ; Deoxyribonuclease ; Deoxyribonucleic acid ; DNA ; Dornase alfa ; Ear diseases ; Effusion ; Fluorescence ; Hospitals ; Humans ; Male ; Medical research ; Medicine ; Middle ear ; Mucosa ; Nasopharyngeal Diseases - metabolism ; Neutrophils ; Neutrophils - metabolism ; Otitis media ; Otitis Media - metabolism ; Otitis Media - microbiology ; Otitis media with effusion ; Otitis Media with Effusion - metabolism ; Otitis Media with Effusion - microbiology ; Otolaryngology ; Recurrence (Disease) ; Staining ; Stranding ; Strands ; Streptococcus infections ; Surgery ; Therapeutic applications ; Viability ; Viscosity ; Young Adult</subject><ispartof>PloS one, 2013-02, Vol.8 (2), p.e53837-e53837</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Thornton et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Thornton et al 2013 Thornton et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a304a37fdce6398525aae029ca599ff9820aedc7dfd73b5b3f8f95e04ecc1aad3</citedby><cites>FETCH-LOGICAL-c692t-a304a37fdce6398525aae029ca599ff9820aedc7dfd73b5b3f8f95e04ecc1aad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564866/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564866/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23393551$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thornton, Ruth B</creatorcontrib><creatorcontrib>Wiertsema, Selma P</creatorcontrib><creatorcontrib>Kirkham, Lea-Ann S</creatorcontrib><creatorcontrib>Rigby, Paul J</creatorcontrib><creatorcontrib>Vijayasekaran, Shyan</creatorcontrib><creatorcontrib>Coates, Harvey L</creatorcontrib><creatorcontrib>Richmond, Peter C</creatorcontrib><title>Neutrophil extracellular traps and bacterial biofilms in middle ear effusion of children with recurrent acute otitis media--a potential treatment target</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Bacteria persist within biofilms on the middle ear mucosa of children with recurrent and chronic otitis media however the mechanisms by which these develop remain to be elucidated. Biopsies can be difficult to obtain from children and their small size limits analysis.
In this study we aimed to investigate biofilm presence in middle ear effusion (MEE) from children with recurrent acute otitis media (rAOM) and to determine if these may represent infectious reservoirs similarly to those on the mucosa. We examined this through culture, viability staining and fluorescent in situ hybridisation (FISH) to determine bacterial species present. Most MEEs had live bacteria present using viability staining (32/36) and all effusions had bacteria present using the universal FISH probe (26/26). Of these, 70% contained 2 or more otopathogenic species. Extensive DNA stranding was also present. This DNA was largely host derived, representing neutrophil extracellular traps (NETs) within which live bacteria in biofilm formations were present. When treated with the recombinant human deoxyribonuclease 1, Dornase alfa, these strands were observed to fragment.
Bacterial biofilms, composed of multiple live otopathogenic species can be demonstrated in the MEEs of children with rAOM and that these contain extensive DNA stranding from NETs. The NETs contribute to the viscosity of the effusion, potentially contributing to its failure to clear as well as biofilm development. 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metabolism</subject><subject>Otitis Media - microbiology</subject><subject>Otitis media with effusion</subject><subject>Otitis Media with Effusion - metabolism</subject><subject>Otitis Media with Effusion - microbiology</subject><subject>Otolaryngology</subject><subject>Recurrence (Disease)</subject><subject>Staining</subject><subject>Stranding</subject><subject>Strands</subject><subject>Streptococcus infections</subject><subject>Surgery</subject><subject>Therapeutic applications</subject><subject>Viability</subject><subject>Viscosity</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tq3DAQhk1padK0b1BaQaG0F7u1LMuybwoh9BAIDfR0K8bSaFdBtjaS3MOb9HErZzchW3JRDLZsffPLM_NPUTyl5ZIyQd9c-CmM4JYbP-KyLDlrmbhXHNKOVYumKtn9W-uD4lGMF1dQ0zwsDirGOsY5PSz-fMIpBb9ZW0fwVwqg0LnJQSB5vYkERk16UAmDBUd66411QyR2JIPV2iHBjKIxU7R-JN4QlZV0wJH8tGlNAqop5LdEQE0JiU822UgG1BYWCyAbn_LmLJ0CQhpmMkFYYXpcPDDgIj7ZPY-Kb-_ffT35uDg7_3B6cny2UE1XpQWwsgYmjFbYsK7lFQfAsuoU8K4zpmurElAroY0WrOc9M63pOJY1KkUBNDsqnm91N85HuStqlJTVZSm6fMvE6ZbQHi7kJtgBwm_pwcqrDz6sJIRklUNJaWt62grQnNWmNCAqAV3dty0ILkyftd7uTpv6XASV8w3g9kT3d0a7liv_QzLe1Ll3WeDVTiD4ywljkoONc89gRD_l_65aXjc162b0xT_o3dntqBXkBOxo_GyCWVQe16Ktqagpz9TyDipfGgersgOzLXA_4PVeQGZS9tcKphjl6ZfP_8-ef99nX95i1wguraN3U8r2i_tgvQVV8DEGNDdFpqWcB-i6GnIeILkboBz27HaDboKuJ4b9BdPDGiA</recordid><startdate>20130205</startdate><enddate>20130205</enddate><creator>Thornton, Ruth B</creator><creator>Wiertsema, Selma P</creator><creator>Kirkham, Lea-Ann S</creator><creator>Rigby, Paul J</creator><creator>Vijayasekaran, Shyan</creator><creator>Coates, Harvey L</creator><creator>Richmond, Peter C</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130205</creationdate><title>Neutrophil extracellular traps and bacterial biofilms in middle ear effusion of children with recurrent acute otitis media--a potential treatment target</title><author>Thornton, Ruth B ; Wiertsema, Selma P ; Kirkham, Lea-Ann S ; Rigby, Paul J ; Vijayasekaran, Shyan ; Coates, Harvey L ; Richmond, Peter C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-a304a37fdce6398525aae029ca599ff9820aedc7dfd73b5b3f8f95e04ecc1aad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Bacteria</topic><topic>Biofilms</topic><topic>Biofilms - 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Biopsies can be difficult to obtain from children and their small size limits analysis.
In this study we aimed to investigate biofilm presence in middle ear effusion (MEE) from children with recurrent acute otitis media (rAOM) and to determine if these may represent infectious reservoirs similarly to those on the mucosa. We examined this through culture, viability staining and fluorescent in situ hybridisation (FISH) to determine bacterial species present. Most MEEs had live bacteria present using viability staining (32/36) and all effusions had bacteria present using the universal FISH probe (26/26). Of these, 70% contained 2 or more otopathogenic species. Extensive DNA stranding was also present. This DNA was largely host derived, representing neutrophil extracellular traps (NETs) within which live bacteria in biofilm formations were present. When treated with the recombinant human deoxyribonuclease 1, Dornase alfa, these strands were observed to fragment.
Bacterial biofilms, composed of multiple live otopathogenic species can be demonstrated in the MEEs of children with rAOM and that these contain extensive DNA stranding from NETs. The NETs contribute to the viscosity of the effusion, potentially contributing to its failure to clear as well as biofilm development. Our data indicates that Dornase alfa can fragment these strands and may play a role in future chronic OM treatment.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23393551</pmid><doi>10.1371/journal.pone.0053837</doi><tpages>e53837</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adolescent Adult Bacteria Biofilms Biofilms - growth & development Biology Biopsy Children Children & youth Childrens health Cystic fibrosis Deoxyribonuclease Deoxyribonucleic acid DNA Dornase alfa Ear diseases Effusion Fluorescence Hospitals Humans Male Medical research Medicine Middle ear Mucosa Nasopharyngeal Diseases - metabolism Neutrophils Neutrophils - metabolism Otitis media Otitis Media - metabolism Otitis Media - microbiology Otitis media with effusion Otitis Media with Effusion - metabolism Otitis Media with Effusion - microbiology Otolaryngology Recurrence (Disease) Staining Stranding Strands Streptococcus infections Surgery Therapeutic applications Viability Viscosity Young Adult |
| title | Neutrophil extracellular traps and bacterial biofilms in middle ear effusion of children with recurrent acute otitis media--a potential treatment target |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T04%3A30%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neutrophil%20extracellular%20traps%20and%20bacterial%20biofilms%20in%20middle%20ear%20effusion%20of%20children%20with%20recurrent%20acute%20otitis%20media--a%20potential%20treatment%20target&rft.jtitle=PloS%20one&rft.au=Thornton,%20Ruth%20B&rft.date=2013-02-05&rft.volume=8&rft.issue=2&rft.spage=e53837&rft.epage=e53837&rft.pages=e53837-e53837&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0053837&rft_dat=%3Cgale_plos_%3EA478417415%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1340079400&rft_id=info:pmid/23393551&rft_galeid=A478417415&rft_doaj_id=oai_doaj_org_article_118fb187ad534f0fa727a94b88a757fb&rfr_iscdi=true |