Identification of a novel jasmonate-responsive element in the AtJMT promoter and its binding protein for AtJMT repression
Jasmonates (JAs) are important regulators of plant biotic and abiotic stress responses and development. AtJMT in Arabidopsis thaliana and BcNTR1 in Brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (MeJA) biosynthesis and are involved in JA signali...
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| creator | Seo, Jun Sung Koo, Yeon Jong Jung, Choonkyun Yeu, Song Yion Song, Jong Tae Kim, Ju-Kon Choi, Yeonhee Lee, Jong Seob Do Choi, Yang |
| description | Jasmonates (JAs) are important regulators of plant biotic and abiotic stress responses and development. AtJMT in Arabidopsis thaliana and BcNTR1 in Brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (MeJA) biosynthesis and are involved in JA signaling. Their expression is induced by MeJA application. To understand its regulatory mechanism, here we define a novel JA-responsive cis-element (JARE), G(C)TCCTGA, in the AtJMT and BcNTR1 promoters, by promoter deletion analysis and Yeast 1-Hybrid (Y1H) assays; the JARE is distinct from other JA-responsive cis-elements previously reported. We also used Y1H screening to identify a trans-acting factor, AtBBD1, which binds to the JARE and interacts with AtJAZ1 and AtJAZ4. Knockout and overexpression analyses showed that AtBBD1 and its close homologue AtBBD2 are functionally redundant and act as negative regulators of AtJMT expression. However, AtBBD1 positively regulated the JA-responsive expression of JR2. Chromatin immunoprecipitation from knockout and overexpression plants revealed that repression of AtJMT is associated with reduced histone acetylation in the promoter region containing the JARE. These results show that AtBBD1 interacts with JAZ proteins, binds to the JARE and represses AtJMT expression. |
| doi_str_mv | 10.1371/journal.pone.0055482 |
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AtJMT in Arabidopsis thaliana and BcNTR1 in Brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (MeJA) biosynthesis and are involved in JA signaling. Their expression is induced by MeJA application. To understand its regulatory mechanism, here we define a novel JA-responsive cis-element (JARE), G(C)TCCTGA, in the AtJMT and BcNTR1 promoters, by promoter deletion analysis and Yeast 1-Hybrid (Y1H) assays; the JARE is distinct from other JA-responsive cis-elements previously reported. We also used Y1H screening to identify a trans-acting factor, AtBBD1, which binds to the JARE and interacts with AtJAZ1 and AtJAZ4. Knockout and overexpression analyses showed that AtBBD1 and its close homologue AtBBD2 are functionally redundant and act as negative regulators of AtJMT expression. However, AtBBD1 positively regulated the JA-responsive expression of JR2. Chromatin immunoprecipitation from knockout and overexpression plants revealed that repression of AtJMT is associated with reduced histone acetylation in the promoter region containing the JARE. These results show that AtBBD1 interacts with JAZ proteins, binds to the JARE and represses AtJMT expression.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0055482</identifier><identifier>PMID: 23393583</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acetylation ; Agricultural biotechnology ; Analysis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Biology ; Biosynthesis ; Brassica ; Brassica campestris ; Carrier Proteins ; Cell cycle ; Chromatin ; Cyclopentanes - metabolism ; Deoxyribonucleic acid ; DNA ; DNA methylation ; Gene deletion ; Gene expression ; Homology ; Immunoprecipitation ; Jasmonic acid ; Jasmonic acid carboxyl ; Metabolism ; Methyl jasmonate ; Methyltransferases ; Oxylipins - metabolism ; Physiological aspects ; Plants (botany) ; Promoter Regions, Genetic - genetics ; Protein binding ; Proteins ; Regulators ; Regulatory mechanisms (biology) ; Regulatory sequences ; Response Elements - genetics ; Signal transduction ; Signaling ; Yeast</subject><ispartof>PloS one, 2013-02, Vol.8 (2), p.e55482-e55482</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Seo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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AtJMT in Arabidopsis thaliana and BcNTR1 in Brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (MeJA) biosynthesis and are involved in JA signaling. Their expression is induced by MeJA application. To understand its regulatory mechanism, here we define a novel JA-responsive cis-element (JARE), G(C)TCCTGA, in the AtJMT and BcNTR1 promoters, by promoter deletion analysis and Yeast 1-Hybrid (Y1H) assays; the JARE is distinct from other JA-responsive cis-elements previously reported. We also used Y1H screening to identify a trans-acting factor, AtBBD1, which binds to the JARE and interacts with AtJAZ1 and AtJAZ4. Knockout and overexpression analyses showed that AtBBD1 and its close homologue AtBBD2 are functionally redundant and act as negative regulators of AtJMT expression. However, AtBBD1 positively regulated the JA-responsive expression of JR2. 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AtJMT in Arabidopsis thaliana and BcNTR1 in Brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (MeJA) biosynthesis and are involved in JA signaling. Their expression is induced by MeJA application. To understand its regulatory mechanism, here we define a novel JA-responsive cis-element (JARE), G(C)TCCTGA, in the AtJMT and BcNTR1 promoters, by promoter deletion analysis and Yeast 1-Hybrid (Y1H) assays; the JARE is distinct from other JA-responsive cis-elements previously reported. We also used Y1H screening to identify a trans-acting factor, AtBBD1, which binds to the JARE and interacts with AtJAZ1 and AtJAZ4. Knockout and overexpression analyses showed that AtBBD1 and its close homologue AtBBD2 are functionally redundant and act as negative regulators of AtJMT expression. However, AtBBD1 positively regulated the JA-responsive expression of JR2. Chromatin immunoprecipitation from knockout and overexpression plants revealed that repression of AtJMT is associated with reduced histone acetylation in the promoter region containing the JARE. These results show that AtBBD1 interacts with JAZ proteins, binds to the JARE and represses AtJMT expression.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23393583</pmid><doi>10.1371/journal.pone.0055482</doi><tpages>e55482</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylation Agricultural biotechnology Analysis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Biology Biosynthesis Brassica Brassica campestris Carrier Proteins Cell cycle Chromatin Cyclopentanes - metabolism Deoxyribonucleic acid DNA DNA methylation Gene deletion Gene expression Homology Immunoprecipitation Jasmonic acid Jasmonic acid carboxyl Metabolism Methyl jasmonate Methyltransferases Oxylipins - metabolism Physiological aspects Plants (botany) Promoter Regions, Genetic - genetics Protein binding Proteins Regulators Regulatory mechanisms (biology) Regulatory sequences Response Elements - genetics Signal transduction Signaling Yeast |
| title | Identification of a novel jasmonate-responsive element in the AtJMT promoter and its binding protein for AtJMT repression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T18%3A15%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20a%20novel%20jasmonate-responsive%20element%20in%20the%20AtJMT%20promoter%20and%20its%20binding%20protein%20for%20AtJMT%20repression&rft.jtitle=PloS%20one&rft.au=Seo,%20Jun%20Sung&rft.date=2013-02-05&rft.volume=8&rft.issue=2&rft.spage=e55482&rft.epage=e55482&rft.pages=e55482-e55482&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0055482&rft_dat=%3Cgale_plos_%3EA478417439%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1340079264&rft_id=info:pmid/23393583&rft_galeid=A478417439&rft_doaj_id=oai_doaj_org_article_55d7fb9177d943ef9dc825b0e0a6ecd2&rfr_iscdi=true |