Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms
Health control is a major issue in animal breeding and a better knowledge of the genetic bases of resistance to diseases is needed in farm animals including fish. The detection of quantitative trait loci (QTL) will help uncovering the genetic architecture of important traits and understanding the me...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-02, Vol.8 (2), p.e55302-e55302 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e55302 |
|---|---|
| container_issue | 2 |
| container_start_page | e55302 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Verrier, Eloi R Dorson, Michel Mauger, Stéphane Torhy, Corinne Ciobotaru, Céline Hervet, Caroline Dechamp, Nicolas Genet, Carine Boudinot, Pierre Quillet, Edwige |
| description | Health control is a major issue in animal breeding and a better knowledge of the genetic bases of resistance to diseases is needed in farm animals including fish. The detection of quantitative trait loci (QTL) will help uncovering the genetic architecture of important traits and understanding the mechanisms involved in resistance to pathogens. We report here the detection of QTL for resistance to Viral Haemorrhagic Septicaemia Virus (VHSV), a major threat for European aquaculture industry. Two induced mitogynogenetic doubled haploid F2 rainbow trout (Oncorhynchus mykiss) families were used. These families combined the genome of susceptible and resistant F0 breeders and contained only fully homozygous individuals. For phenotyping, fish survival after an immersion challenge with the virus was recorded, as well as in vitro virus replication on fin explants. A bidirectional selective genotyping strategy identified seven QTL associated to survival. One of those QTL was significant at the genome-wide level and largely explained both survival and viral replication in fin explants in the different families of the design (up to 65% and 49% of phenotypic variance explained respectively). These results evidence the key role of innate defence in resistance to the virus and pave the way for the identification of the gene(s) responsible for resistance. The identification of a major QTL also opens appealing perspectives for selective breeding of fish with improved resistance. |
| doi_str_mv | 10.1371/journal.pone.0055302 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1339960455</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478431648</galeid><doaj_id>oai_doaj_org_article_b7114b502efc459b90980e76b87ffa16</doaj_id><sourcerecordid>A478431648</sourcerecordid><originalsourceid>FETCH-LOGICAL-c627t-60484fa736b1c1610b14548c9cfe960832cb1a6c24c0fab53b0ccc6acab1baf03</originalsourceid><addsrcrecordid>eNptUlFv0zAYjBCIbYV_gMASL9tDix07TsIDUjUBnVQJAWOv1mfHbl0ldmcnRfv3uDSb1mnyQ6wvd2ff-bLsHcEzQkvyaeOH4KCdbb3TM4yLguL8RXZKappPeY7py0f7k-wsxk0C0Yrz19lJTmmNi5yfZv6Xjjb24JRGvUeAwhpk43c2DBGd3yx-31wg61AA66T_i_rgh_4zso12vTVWQW-9Q94kYgcbH9DP6yUKuoVeN3s961zaok6rNTgbu_gme2Wgjfrt-J1kf759vb5cTJc_vl9dzpdTxfOyn3LMKmagpFwSRTjBkrCCVapWRtccVzRXkgBXOVPYgCyoxEopDgokkWAwnWQfDrrb1kcxZhUFScYTn6W0JtnVAdF42IhtsB2EO-HBiv8DH1YCQm9Vq4UsCWGywLk2ihW1rHFdYV1yWZXGAOFJ68t42iA73aiUToD2SPT4j7NrsfI7QQtO08MlgYuDwPoJbTFfiv0ME4xJzumOJOz5eFjwt4OOvehsVLptwWk_JI95VTBe8OR1kn18An0-iRG1gmTWOuPTHdVeVMxZWTFKOKsSavYMKq1Gd1alEhqb5kcEdiCo4GMM2jwYI1jsK3x_GbGvsBgrnGjvH2f5QLrvLP0H1UDs-Q</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1339960455</pqid></control><display><type>article</type><title>Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms</title><source>MEDLINE</source><source>PLoS_OA刊</source><source>PubMed Central</source><source>Directory of Open Access Journals</source><source>Free Full-Text Journals in Chemistry</source><source>EZB Electronic Journals Library</source><creator>Verrier, Eloi R ; Dorson, Michel ; Mauger, Stéphane ; Torhy, Corinne ; Ciobotaru, Céline ; Hervet, Caroline ; Dechamp, Nicolas ; Genet, Carine ; Boudinot, Pierre ; Quillet, Edwige</creator><creatorcontrib>Verrier, Eloi R ; Dorson, Michel ; Mauger, Stéphane ; Torhy, Corinne ; Ciobotaru, Céline ; Hervet, Caroline ; Dechamp, Nicolas ; Genet, Carine ; Boudinot, Pierre ; Quillet, Edwige</creatorcontrib><description>Health control is a major issue in animal breeding and a better knowledge of the genetic bases of resistance to diseases is needed in farm animals including fish. The detection of quantitative trait loci (QTL) will help uncovering the genetic architecture of important traits and understanding the mechanisms involved in resistance to pathogens. We report here the detection of QTL for resistance to Viral Haemorrhagic Septicaemia Virus (VHSV), a major threat for European aquaculture industry. Two induced mitogynogenetic doubled haploid F2 rainbow trout (Oncorhynchus mykiss) families were used. These families combined the genome of susceptible and resistant F0 breeders and contained only fully homozygous individuals. For phenotyping, fish survival after an immersion challenge with the virus was recorded, as well as in vitro virus replication on fin explants. A bidirectional selective genotyping strategy identified seven QTL associated to survival. One of those QTL was significant at the genome-wide level and largely explained both survival and viral replication in fin explants in the different families of the design (up to 65% and 49% of phenotypic variance explained respectively). These results evidence the key role of innate defence in resistance to the virus and pave the way for the identification of the gene(s) responsible for resistance. The identification of a major QTL also opens appealing perspectives for selective breeding of fish with improved resistance.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0055302</identifier><identifier>PMID: 23390526</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Animal breeding ; Animal husbandry ; Animals ; Aquaculture ; Biology ; Chromosome Mapping ; Disease resistance ; Explants ; Female ; Fish ; Fish diseases ; Gene mapping ; Genetic aspects ; Genome ; Genomes ; Genomics ; Genotype ; Genotyping ; Hemorrhagic septicemia ; Hemorrhagic Septicemia, Viral - genetics ; Hemorrhagic Septicemia, Viral - immunology ; Hemorrhagic Septicemia, Viral - mortality ; Hemorrhagic Septicemia, Viral - virology ; Homozygote ; Immunity, Innate - genetics ; Life Sciences ; Male ; Microbiology and Parasitology ; Novirhabdovirus - physiology ; Oncorhynchus mykiss ; Oncorhynchus mykiss - genetics ; Oncorhynchus mykiss - immunology ; Oncorhynchus mykiss - virology ; Phenotype ; Phenotyping ; Physiological aspects ; Quantitative trait loci ; Quantitative Trait Loci - immunology ; Rainbow trout ; Replication ; Rhabdoviruses ; Salmon ; Selective breeding ; Survival ; Survival Rate ; Tissue Culture Techniques ; Trout ; Veterinary Science ; Virus Replication ; Viruses ; Zebrafish</subject><ispartof>PloS one, 2013-02, Vol.8 (2), p.e55302-e55302</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Verrier et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Attribution</rights><rights>2013 Verrier et al 2013 Verrier et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c627t-60484fa736b1c1610b14548c9cfe960832cb1a6c24c0fab53b0ccc6acab1baf03</citedby><cites>FETCH-LOGICAL-c627t-60484fa736b1c1610b14548c9cfe960832cb1a6c24c0fab53b0ccc6acab1baf03</cites><orcidid>0000-0003-3248-2182 ; 0000-0002-7490-9677 ; 0000-0002-3734-9017</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563530/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563530/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23390526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01001263$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Verrier, Eloi R</creatorcontrib><creatorcontrib>Dorson, Michel</creatorcontrib><creatorcontrib>Mauger, Stéphane</creatorcontrib><creatorcontrib>Torhy, Corinne</creatorcontrib><creatorcontrib>Ciobotaru, Céline</creatorcontrib><creatorcontrib>Hervet, Caroline</creatorcontrib><creatorcontrib>Dechamp, Nicolas</creatorcontrib><creatorcontrib>Genet, Carine</creatorcontrib><creatorcontrib>Boudinot, Pierre</creatorcontrib><creatorcontrib>Quillet, Edwige</creatorcontrib><title>Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Health control is a major issue in animal breeding and a better knowledge of the genetic bases of resistance to diseases is needed in farm animals including fish. The detection of quantitative trait loci (QTL) will help uncovering the genetic architecture of important traits and understanding the mechanisms involved in resistance to pathogens. We report here the detection of QTL for resistance to Viral Haemorrhagic Septicaemia Virus (VHSV), a major threat for European aquaculture industry. Two induced mitogynogenetic doubled haploid F2 rainbow trout (Oncorhynchus mykiss) families were used. These families combined the genome of susceptible and resistant F0 breeders and contained only fully homozygous individuals. For phenotyping, fish survival after an immersion challenge with the virus was recorded, as well as in vitro virus replication on fin explants. A bidirectional selective genotyping strategy identified seven QTL associated to survival. One of those QTL was significant at the genome-wide level and largely explained both survival and viral replication in fin explants in the different families of the design (up to 65% and 49% of phenotypic variance explained respectively). These results evidence the key role of innate defence in resistance to the virus and pave the way for the identification of the gene(s) responsible for resistance. The identification of a major QTL also opens appealing perspectives for selective breeding of fish with improved resistance.</description><subject>Agriculture</subject><subject>Animal breeding</subject><subject>Animal husbandry</subject><subject>Animals</subject><subject>Aquaculture</subject><subject>Biology</subject><subject>Chromosome Mapping</subject><subject>Disease resistance</subject><subject>Explants</subject><subject>Female</subject><subject>Fish</subject><subject>Fish diseases</subject><subject>Gene mapping</subject><subject>Genetic aspects</subject><subject>Genome</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Genotype</subject><subject>Genotyping</subject><subject>Hemorrhagic septicemia</subject><subject>Hemorrhagic Septicemia, Viral - genetics</subject><subject>Hemorrhagic Septicemia, Viral - immunology</subject><subject>Hemorrhagic Septicemia, Viral - mortality</subject><subject>Hemorrhagic Septicemia, Viral - virology</subject><subject>Homozygote</subject><subject>Immunity, Innate - genetics</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Microbiology and Parasitology</subject><subject>Novirhabdovirus - physiology</subject><subject>Oncorhynchus mykiss</subject><subject>Oncorhynchus mykiss - genetics</subject><subject>Oncorhynchus mykiss - immunology</subject><subject>Oncorhynchus mykiss - virology</subject><subject>Phenotype</subject><subject>Phenotyping</subject><subject>Physiological aspects</subject><subject>Quantitative trait loci</subject><subject>Quantitative Trait Loci - immunology</subject><subject>Rainbow trout</subject><subject>Replication</subject><subject>Rhabdoviruses</subject><subject>Salmon</subject><subject>Selective breeding</subject><subject>Survival</subject><subject>Survival Rate</subject><subject>Tissue Culture Techniques</subject><subject>Trout</subject><subject>Veterinary Science</subject><subject>Virus Replication</subject><subject>Viruses</subject><subject>Zebrafish</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUlFv0zAYjBCIbYV_gMASL9tDix07TsIDUjUBnVQJAWOv1mfHbl0ldmcnRfv3uDSb1mnyQ6wvd2ff-bLsHcEzQkvyaeOH4KCdbb3TM4yLguL8RXZKappPeY7py0f7k-wsxk0C0Yrz19lJTmmNi5yfZv6Xjjb24JRGvUeAwhpk43c2DBGd3yx-31wg61AA66T_i_rgh_4zso12vTVWQW-9Q94kYgcbH9DP6yUKuoVeN3s961zaok6rNTgbu_gme2Wgjfrt-J1kf759vb5cTJc_vl9dzpdTxfOyn3LMKmagpFwSRTjBkrCCVapWRtccVzRXkgBXOVPYgCyoxEopDgokkWAwnWQfDrrb1kcxZhUFScYTn6W0JtnVAdF42IhtsB2EO-HBiv8DH1YCQm9Vq4UsCWGywLk2ihW1rHFdYV1yWZXGAOFJ68t42iA73aiUToD2SPT4j7NrsfI7QQtO08MlgYuDwPoJbTFfiv0ME4xJzumOJOz5eFjwt4OOvehsVLptwWk_JI95VTBe8OR1kn18An0-iRG1gmTWOuPTHdVeVMxZWTFKOKsSavYMKq1Gd1alEhqb5kcEdiCo4GMM2jwYI1jsK3x_GbGvsBgrnGjvH2f5QLrvLP0H1UDs-Q</recordid><startdate>20130204</startdate><enddate>20130204</enddate><creator>Verrier, Eloi R</creator><creator>Dorson, Michel</creator><creator>Mauger, Stéphane</creator><creator>Torhy, Corinne</creator><creator>Ciobotaru, Céline</creator><creator>Hervet, Caroline</creator><creator>Dechamp, Nicolas</creator><creator>Genet, Carine</creator><creator>Boudinot, Pierre</creator><creator>Quillet, Edwige</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3248-2182</orcidid><orcidid>https://orcid.org/0000-0002-7490-9677</orcidid><orcidid>https://orcid.org/0000-0002-3734-9017</orcidid></search><sort><creationdate>20130204</creationdate><title>Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms</title><author>Verrier, Eloi R ; Dorson, Michel ; Mauger, Stéphane ; Torhy, Corinne ; Ciobotaru, Céline ; Hervet, Caroline ; Dechamp, Nicolas ; Genet, Carine ; Boudinot, Pierre ; Quillet, Edwige</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c627t-60484fa736b1c1610b14548c9cfe960832cb1a6c24c0fab53b0ccc6acab1baf03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Agriculture</topic><topic>Animal breeding</topic><topic>Animal husbandry</topic><topic>Animals</topic><topic>Aquaculture</topic><topic>Biology</topic><topic>Chromosome Mapping</topic><topic>Disease resistance</topic><topic>Explants</topic><topic>Female</topic><topic>Fish</topic><topic>Fish diseases</topic><topic>Gene mapping</topic><topic>Genetic aspects</topic><topic>Genome</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Genotype</topic><topic>Genotyping</topic><topic>Hemorrhagic septicemia</topic><topic>Hemorrhagic Septicemia, Viral - genetics</topic><topic>Hemorrhagic Septicemia, Viral - immunology</topic><topic>Hemorrhagic Septicemia, Viral - mortality</topic><topic>Hemorrhagic Septicemia, Viral - virology</topic><topic>Homozygote</topic><topic>Immunity, Innate - genetics</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Microbiology and Parasitology</topic><topic>Novirhabdovirus - physiology</topic><topic>Oncorhynchus mykiss</topic><topic>Oncorhynchus mykiss - genetics</topic><topic>Oncorhynchus mykiss - immunology</topic><topic>Oncorhynchus mykiss - virology</topic><topic>Phenotype</topic><topic>Phenotyping</topic><topic>Physiological aspects</topic><topic>Quantitative trait loci</topic><topic>Quantitative Trait Loci - immunology</topic><topic>Rainbow trout</topic><topic>Replication</topic><topic>Rhabdoviruses</topic><topic>Salmon</topic><topic>Selective breeding</topic><topic>Survival</topic><topic>Survival Rate</topic><topic>Tissue Culture Techniques</topic><topic>Trout</topic><topic>Veterinary Science</topic><topic>Virus Replication</topic><topic>Viruses</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verrier, Eloi R</creatorcontrib><creatorcontrib>Dorson, Michel</creatorcontrib><creatorcontrib>Mauger, Stéphane</creatorcontrib><creatorcontrib>Torhy, Corinne</creatorcontrib><creatorcontrib>Ciobotaru, Céline</creatorcontrib><creatorcontrib>Hervet, Caroline</creatorcontrib><creatorcontrib>Dechamp, Nicolas</creatorcontrib><creatorcontrib>Genet, Carine</creatorcontrib><creatorcontrib>Boudinot, Pierre</creatorcontrib><creatorcontrib>Quillet, Edwige</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Database (1962 - current)</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest advanced technologies & aerospace journals</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verrier, Eloi R</au><au>Dorson, Michel</au><au>Mauger, Stéphane</au><au>Torhy, Corinne</au><au>Ciobotaru, Céline</au><au>Hervet, Caroline</au><au>Dechamp, Nicolas</au><au>Genet, Carine</au><au>Boudinot, Pierre</au><au>Quillet, Edwige</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-02-04</date><risdate>2013</risdate><volume>8</volume><issue>2</issue><spage>e55302</spage><epage>e55302</epage><pages>e55302-e55302</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Health control is a major issue in animal breeding and a better knowledge of the genetic bases of resistance to diseases is needed in farm animals including fish. The detection of quantitative trait loci (QTL) will help uncovering the genetic architecture of important traits and understanding the mechanisms involved in resistance to pathogens. We report here the detection of QTL for resistance to Viral Haemorrhagic Septicaemia Virus (VHSV), a major threat for European aquaculture industry. Two induced mitogynogenetic doubled haploid F2 rainbow trout (Oncorhynchus mykiss) families were used. These families combined the genome of susceptible and resistant F0 breeders and contained only fully homozygous individuals. For phenotyping, fish survival after an immersion challenge with the virus was recorded, as well as in vitro virus replication on fin explants. A bidirectional selective genotyping strategy identified seven QTL associated to survival. One of those QTL was significant at the genome-wide level and largely explained both survival and viral replication in fin explants in the different families of the design (up to 65% and 49% of phenotypic variance explained respectively). These results evidence the key role of innate defence in resistance to the virus and pave the way for the identification of the gene(s) responsible for resistance. The identification of a major QTL also opens appealing perspectives for selective breeding of fish with improved resistance.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23390526</pmid><doi>10.1371/journal.pone.0055302</doi><orcidid>https://orcid.org/0000-0003-3248-2182</orcidid><orcidid>https://orcid.org/0000-0002-7490-9677</orcidid><orcidid>https://orcid.org/0000-0002-3734-9017</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-02, Vol.8 (2), p.e55302-e55302 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1339960455 |
| source | MEDLINE; PLoS_OA刊; PubMed Central; Directory of Open Access Journals; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Agriculture Animal breeding Animal husbandry Animals Aquaculture Biology Chromosome Mapping Disease resistance Explants Female Fish Fish diseases Gene mapping Genetic aspects Genome Genomes Genomics Genotype Genotyping Hemorrhagic septicemia Hemorrhagic Septicemia, Viral - genetics Hemorrhagic Septicemia, Viral - immunology Hemorrhagic Septicemia, Viral - mortality Hemorrhagic Septicemia, Viral - virology Homozygote Immunity, Innate - genetics Life Sciences Male Microbiology and Parasitology Novirhabdovirus - physiology Oncorhynchus mykiss Oncorhynchus mykiss - genetics Oncorhynchus mykiss - immunology Oncorhynchus mykiss - virology Phenotype Phenotyping Physiological aspects Quantitative trait loci Quantitative Trait Loci - immunology Rainbow trout Replication Rhabdoviruses Salmon Selective breeding Survival Survival Rate Tissue Culture Techniques Trout Veterinary Science Virus Replication Viruses Zebrafish |
| title | Resistance to a rhabdovirus (VHSV) in rainbow trout: identification of a major QTL related to innate mechanisms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T07%3A34%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Resistance%20to%20a%20rhabdovirus%20(VHSV)%20in%20rainbow%20trout:%20identification%20of%20a%20major%20QTL%20related%20to%20innate%20mechanisms&rft.jtitle=PloS%20one&rft.au=Verrier,%20Eloi%20R&rft.date=2013-02-04&rft.volume=8&rft.issue=2&rft.spage=e55302&rft.epage=e55302&rft.pages=e55302-e55302&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0055302&rft_dat=%3Cgale_plos_%3EA478431648%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1339960455&rft_id=info:pmid/23390526&rft_galeid=A478431648&rft_doaj_id=oai_doaj_org_article_b7114b502efc459b90980e76b87ffa16&rfr_iscdi=true |