Identification of suitable reference genes for real-time PCR analysis of statin-treated human umbilical vein endothelial cells

Proper data normalization in quantitative real-time reverse-transcription polymerase chain reaction (RT-qPCR) is of critical importance for reliable mRNA expression analysis. Due to a diversity in putative reference genes expression stability in different in vitro models, a validation of an internal...

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Veröffentlicht in:	PloS one 2012-12, Vol.7 (12), p.e51547-e51547
Hauptverfasser:	Żyżyńska-Granica, Barbara, Koziak, Katarzyna
Format:	Artikel
Sprache:	eng
Schlagworte:	Actin Analysis Biochemistry Biological Assay Biology Cancer therapies Control stability Dehydrogenases Endothelial cells Endothelium Experimental design Experiments Gadd45A protein Gene expression Genes Glyceraldehyde-3-phosphate dehydrogenase Human Umbilical Vein Endothelial Cells - drug effects Human Umbilical Vein Endothelial Cells - metabolism Humans Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology Hypoxanthine Hypoxanthine phosphoribosyltransferase Medical research Medicine Monooxygenase Normalizing Phosphoribosyltransferase Polymerase chain reaction Real time Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Reference Standards RNA Stability analysis Statins Studies Tissue Donors Tryptophan Tryptophan 5-monooxygenase Tumor necrosis factor-TNF Tumor necrosis factor-α Tyrosine Tyrosine 3-monooxygenase Umbilical vein
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