Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis
Feline idiopathic cystitis (FIC) is the only spontaneous animal model for human interstitial cystitis (IC), as both possess a distinctive chronical and relapsing character. Underlying pathomechanisms of both diseases are not clearly established yet. We recently detected increased urine fibronectin l...
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| Veröffentlicht in: | PloS one 2012-12, Vol.7 (12), p.e51391-e51391 |
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| creator | Treutlein, Gudrun Dorsch, Roswitha Euler, Kerstin N Hauck, Stefanie M Amann, Barbara Hartmann, Katrin Deeg, Cornelia A |
| description | Feline idiopathic cystitis (FIC) is the only spontaneous animal model for human interstitial cystitis (IC), as both possess a distinctive chronical and relapsing character. Underlying pathomechanisms of both diseases are not clearly established yet. We recently detected increased urine fibronectin levels in FIC cases. The purpose of this study was to gain further insight into the pathogenesis by assessing interacting partners of fibronectin in urine of FIC affected cats. Several candidate proteins were identified via immunoprecipitation and mass spectrometry. Considerable changes in FIC conditions compared to physiological expression of co-purified proteins were detected by Western blot and immunohistochemistry. Compared to controls, complement C4a and thioredoxin were present in higher levels in urine of FIC patients whereas loss of signal intensity was detected in FIC affected tissue. Galectin-7 was exclusively detected in urine of FIC cats, pointing to an important role of this molecule in FIC pathogenesis. Moderate physiological signal intensity of galectin-7 in transitional epithelium shifted to distinct expression in transitional epithelium under pathophysiological conditions. I-FABP expression was reduced in urine and urinary bladder tissue of FIC cats. Additionally, transduction molecules of thioredoxin, NF-κB p65 and p38 MAPK, were examined. In FIC affected tissue, colocalization of thioredoxin and NF-κB p65 could be demonstrated compared to absent coexpression of thioredoxin and p38 MAPK. These considerable changes in expression level and pattern point to an important role for co-purified proteins of fibronectin and thioredoxin-regulated signal transduction pathways in FIC pathogenesis. These results could provide a promising starting point for novel therapeutic approaches in the future. |
| doi_str_mv | 10.1371/journal.pone.0051391 |
| format | Article |
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Underlying pathomechanisms of both diseases are not clearly established yet. We recently detected increased urine fibronectin levels in FIC cases. The purpose of this study was to gain further insight into the pathogenesis by assessing interacting partners of fibronectin in urine of FIC affected cats. Several candidate proteins were identified via immunoprecipitation and mass spectrometry. Considerable changes in FIC conditions compared to physiological expression of co-purified proteins were detected by Western blot and immunohistochemistry. Compared to controls, complement C4a and thioredoxin were present in higher levels in urine of FIC patients whereas loss of signal intensity was detected in FIC affected tissue. Galectin-7 was exclusively detected in urine of FIC cats, pointing to an important role of this molecule in FIC pathogenesis. Moderate physiological signal intensity of galectin-7 in transitional epithelium shifted to distinct expression in transitional epithelium under pathophysiological conditions. I-FABP expression was reduced in urine and urinary bladder tissue of FIC cats. Additionally, transduction molecules of thioredoxin, NF-κB p65 and p38 MAPK, were examined. In FIC affected tissue, colocalization of thioredoxin and NF-κB p65 could be demonstrated compared to absent coexpression of thioredoxin and p38 MAPK. These considerable changes in expression level and pattern point to an important role for co-purified proteins of fibronectin and thioredoxin-regulated signal transduction pathways in FIC pathogenesis. These results could provide a promising starting point for novel therapeutic approaches in the future.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0051391</identifier><identifier>PMID: 23236492</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Bladder ; Blotting, Western ; Cats ; Cellular signal transduction ; Chromatography, High Pressure Liquid ; Complement C4a - metabolism ; Complement C4a - urine ; Cystitis ; Cystitis, Interstitial - urine ; Disease ; Epithelium ; Fatty acid-binding protein ; Fatty Acid-Binding Proteins - metabolism ; Fatty Acid-Binding Proteins - urine ; Fatty acids ; Fibronectin ; Fibronectins ; Fibronectins - metabolism ; Fibronectins - urine ; Galectins - metabolism ; Galectins - urine ; Gene expression ; Immunohistochemistry ; Immunoprecipitation ; Interstitial cystitis ; Ischemia ; MAP kinase ; Mass spectrometry ; Mass spectroscopy ; Medicine ; NF-κB protein ; p38 Mitogen-Activated Protein Kinases - metabolism ; p38 Mitogen-Activated Protein Kinases - urine ; Pathogenesis ; Physiological aspects ; Physiology ; Proteins ; Rodents ; Tandem Mass Spectrometry ; Thioredoxin ; Thioredoxins ; Thioredoxins - metabolism ; Thioredoxins - urine ; Transcription Factor RelA - metabolism ; Transcription Factor RelA - urine ; Transduction ; Urinalysis ; Urinary bladder ; Urinary Bladder - metabolism ; Urinary tract diseases ; Urinary tract infections ; Urine ; Urogenital system ; Veterinary medicine ; Veterinary Science</subject><ispartof>PloS one, 2012-12, Vol.7 (12), p.e51391-e51391</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Treutlein et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Underlying pathomechanisms of both diseases are not clearly established yet. We recently detected increased urine fibronectin levels in FIC cases. The purpose of this study was to gain further insight into the pathogenesis by assessing interacting partners of fibronectin in urine of FIC affected cats. Several candidate proteins were identified via immunoprecipitation and mass spectrometry. Considerable changes in FIC conditions compared to physiological expression of co-purified proteins were detected by Western blot and immunohistochemistry. Compared to controls, complement C4a and thioredoxin were present in higher levels in urine of FIC patients whereas loss of signal intensity was detected in FIC affected tissue. Galectin-7 was exclusively detected in urine of FIC cats, pointing to an important role of this molecule in FIC pathogenesis. Moderate physiological signal intensity of galectin-7 in transitional epithelium shifted to distinct expression in transitional epithelium under pathophysiological conditions. I-FABP expression was reduced in urine and urinary bladder tissue of FIC cats. Additionally, transduction molecules of thioredoxin, NF-κB p65 and p38 MAPK, were examined. In FIC affected tissue, colocalization of thioredoxin and NF-κB p65 could be demonstrated compared to absent coexpression of thioredoxin and p38 MAPK. These considerable changes in expression level and pattern point to an important role for co-purified proteins of fibronectin and thioredoxin-regulated signal transduction pathways in FIC pathogenesis. 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metabolism</subject><subject>p38 Mitogen-Activated Protein Kinases - urine</subject><subject>Pathogenesis</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Rodents</subject><subject>Tandem Mass Spectrometry</subject><subject>Thioredoxin</subject><subject>Thioredoxins</subject><subject>Thioredoxins - metabolism</subject><subject>Thioredoxins - urine</subject><subject>Transcription Factor RelA - metabolism</subject><subject>Transcription Factor RelA - urine</subject><subject>Transduction</subject><subject>Urinalysis</subject><subject>Urinary bladder</subject><subject>Urinary Bladder - metabolism</subject><subject>Urinary tract diseases</subject><subject>Urinary tract infections</subject><subject>Urine</subject><subject>Urogenital system</subject><subject>Veterinary medicine</subject><subject>Veterinary Science</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11r2zAUhs3YWLtu_2BshsHYLpLpy7Z8MyhlH4Gywr5uxYksJwqK5UpyWf_9ThK3xKMXwxcW0nPeo_cVJ8teUjKnvKIfNn4IHbh57zszJ6SgvKaPslNaczYrGeGPj9Yn2bMYNwhxWZZPsxPGGS9FzU6z9Td_Y1ze-2S6ZMHltksmgE62W-U9hNSZEHPf5q1dBuy0O0Amj9g2QWf8EHPo7BYrt75BJUT3EjHZvZ6-3a_i8-xJCy6aF-P_LPv1-dPPi6-zy6svi4vzy5kua5ZmGnhVCFlU3MiWg66blkoqCl4YIzhhJeENk0QwgEaKQhte1VJIiX4aMGjrLHt90O2dj2oMKSrKeU3KQjCBxOJANB42qg94-XCrPFi13_BhpdC31c6olhUEe7XUSCkaKSWUYABKwZa8WQqJWh_HbsNyaxqNIQZwE9HpSWfXauVvFC9oJWqKAu9GgeCvBxOT2tqojXOHbBVleO-iIoQg-uYf9GF3I7UCNGC71mNfvRNV56KqiBQoidT8AQq_xmytxnduLe5PCt5PCpBJ5k9awRCjWvz4_v_s1e8p-_aIXRtwaR29G5L1XZyC4gDq4GMMpr0PmRK1G4i7NNRuINQ4EFj26viB7ovuJoD_BQfOBj4</recordid><startdate>20121207</startdate><enddate>20121207</enddate><creator>Treutlein, Gudrun</creator><creator>Dorsch, Roswitha</creator><creator>Euler, Kerstin N</creator><creator>Hauck, Stefanie M</creator><creator>Amann, Barbara</creator><creator>Hartmann, Katrin</creator><creator>Deeg, Cornelia A</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20121207</creationdate><title>Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis</title><author>Treutlein, Gudrun ; Dorsch, Roswitha ; Euler, Kerstin N ; Hauck, Stefanie M ; Amann, Barbara ; Hartmann, Katrin ; Deeg, Cornelia A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-ca37548573e8f3ac9df1814535ee4302603d28042aad845ce3798488236dae323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Bladder</topic><topic>Blotting, Western</topic><topic>Cats</topic><topic>Cellular signal transduction</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Complement C4a - metabolism</topic><topic>Complement C4a - urine</topic><topic>Cystitis</topic><topic>Cystitis, Interstitial - urine</topic><topic>Disease</topic><topic>Epithelium</topic><topic>Fatty acid-binding protein</topic><topic>Fatty Acid-Binding Proteins - metabolism</topic><topic>Fatty Acid-Binding Proteins - urine</topic><topic>Fatty acids</topic><topic>Fibronectin</topic><topic>Fibronectins</topic><topic>Fibronectins - metabolism</topic><topic>Fibronectins - urine</topic><topic>Galectins - metabolism</topic><topic>Galectins - urine</topic><topic>Gene expression</topic><topic>Immunohistochemistry</topic><topic>Immunoprecipitation</topic><topic>Interstitial cystitis</topic><topic>Ischemia</topic><topic>MAP kinase</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medicine</topic><topic>NF-κB protein</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>p38 Mitogen-Activated Protein Kinases - urine</topic><topic>Pathogenesis</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Proteins</topic><topic>Rodents</topic><topic>Tandem Mass Spectrometry</topic><topic>Thioredoxin</topic><topic>Thioredoxins</topic><topic>Thioredoxins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Treutlein, Gudrun</au><au>Dorsch, Roswitha</au><au>Euler, Kerstin N</au><au>Hauck, Stefanie M</au><au>Amann, Barbara</au><au>Hartmann, Katrin</au><au>Deeg, Cornelia A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2012-12-07</date><risdate>2012</risdate><volume>7</volume><issue>12</issue><spage>e51391</spage><epage>e51391</epage><pages>e51391-e51391</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Feline idiopathic cystitis (FIC) is the only spontaneous animal model for human interstitial cystitis (IC), as both possess a distinctive chronical and relapsing character. Underlying pathomechanisms of both diseases are not clearly established yet. We recently detected increased urine fibronectin levels in FIC cases. The purpose of this study was to gain further insight into the pathogenesis by assessing interacting partners of fibronectin in urine of FIC affected cats. Several candidate proteins were identified via immunoprecipitation and mass spectrometry. Considerable changes in FIC conditions compared to physiological expression of co-purified proteins were detected by Western blot and immunohistochemistry. Compared to controls, complement C4a and thioredoxin were present in higher levels in urine of FIC patients whereas loss of signal intensity was detected in FIC affected tissue. Galectin-7 was exclusively detected in urine of FIC cats, pointing to an important role of this molecule in FIC pathogenesis. Moderate physiological signal intensity of galectin-7 in transitional epithelium shifted to distinct expression in transitional epithelium under pathophysiological conditions. I-FABP expression was reduced in urine and urinary bladder tissue of FIC cats. Additionally, transduction molecules of thioredoxin, NF-κB p65 and p38 MAPK, were examined. In FIC affected tissue, colocalization of thioredoxin and NF-κB p65 could be demonstrated compared to absent coexpression of thioredoxin and p38 MAPK. These considerable changes in expression level and pattern point to an important role for co-purified proteins of fibronectin and thioredoxin-regulated signal transduction pathways in FIC pathogenesis. These results could provide a promising starting point for novel therapeutic approaches in the future.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23236492</pmid><doi>10.1371/journal.pone.0051391</doi><tpages>e51391</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1339065424 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Bladder Blotting, Western Cats Cellular signal transduction Chromatography, High Pressure Liquid Complement C4a - metabolism Complement C4a - urine Cystitis Cystitis, Interstitial - urine Disease Epithelium Fatty acid-binding protein Fatty Acid-Binding Proteins - metabolism Fatty Acid-Binding Proteins - urine Fatty acids Fibronectin Fibronectins Fibronectins - metabolism Fibronectins - urine Galectins - metabolism Galectins - urine Gene expression Immunohistochemistry Immunoprecipitation Interstitial cystitis Ischemia MAP kinase Mass spectrometry Mass spectroscopy Medicine NF-κB protein p38 Mitogen-Activated Protein Kinases - metabolism p38 Mitogen-Activated Protein Kinases - urine Pathogenesis Physiological aspects Physiology Proteins Rodents Tandem Mass Spectrometry Thioredoxin Thioredoxins Thioredoxins - metabolism Thioredoxins - urine Transcription Factor RelA - metabolism Transcription Factor RelA - urine Transduction Urinalysis Urinary bladder Urinary Bladder - metabolism Urinary tract diseases Urinary tract infections Urine Urogenital system Veterinary medicine Veterinary Science |
| title | Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T18%3A53%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20potential%20interacting%20partners%20of%20fibronectin%20in%20spontaneous%20animal%20model%20of%20interstitial%20cystitis&rft.jtitle=PloS%20one&rft.au=Treutlein,%20Gudrun&rft.date=2012-12-07&rft.volume=7&rft.issue=12&rft.spage=e51391&rft.epage=e51391&rft.pages=e51391-e51391&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0051391&rft_dat=%3Cgale_plos_%3EA477084123%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1339065424&rft_id=info:pmid/23236492&rft_galeid=A477084123&rft_doaj_id=oai_doaj_org_article_f250042f1e884d888a6aeaa642b3db48&rfr_iscdi=true |