Investigation of the effect of type 2 diabetes mellitus on subgingival plaque microbiota by high-throughput 16S rDNA pyrosequencing
Diabetes mellitus is a major risk factor for chronic periodontitis. We investigated the effects of type 2 diabetes on the subgingival plaque bacterial composition by applying culture-independent 16S rDNA sequencing to periodontal bacteria isolated from four groups of volunteers: non-diabetic subject...
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| creator | Zhou, Mi Rong, Ruichen Munro, Daniel Zhu, Chunxia Gao, Xiang Zhang, Qi Dong, Qunfeng |
| description | Diabetes mellitus is a major risk factor for chronic periodontitis. We investigated the effects of type 2 diabetes on the subgingival plaque bacterial composition by applying culture-independent 16S rDNA sequencing to periodontal bacteria isolated from four groups of volunteers: non-diabetic subjects without periodontitis, non-diabetic subjects with periodontitis, type 2 diabetic patients without periodontitis, and type 2 diabetic patients with periodontitis. A total of 71,373 high-quality sequences were produced from the V1-V3 region of 16S rDNA genes by 454 pyrosequencing. Those 16S rDNA sequences were classified into 16 phyla, 27 classes, 48 orders, 85 families, 126 genera, and 1141 species-level OTUs. Comparing periodontally healthy samples with periodontitis samples identified 20 health-associated and 15 periodontitis-associated OTUs. In the subjects with healthy periodontium, the abundances of three genera (Prevotella, Pseudomonas, and Tannerella) and nine OTUs were significantly different between diabetic patients and their non-diabetic counterparts. In the subjects carrying periodontitis, the abundances of three phyla (Actinobacteria, Proteobacteria, and Bacteriodetes), two genera (Actinomyces and Aggregatibacter), and six OTUs were also significantly different between diabetics and non-diabetics. Our results show that type 2 diabetes mellitus could alter the bacterial composition in the subgingival plaque. |
| doi_str_mv | 10.1371/journal.pone.0061516 |
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We investigated the effects of type 2 diabetes on the subgingival plaque bacterial composition by applying culture-independent 16S rDNA sequencing to periodontal bacteria isolated from four groups of volunteers: non-diabetic subjects without periodontitis, non-diabetic subjects with periodontitis, type 2 diabetic patients without periodontitis, and type 2 diabetic patients with periodontitis. A total of 71,373 high-quality sequences were produced from the V1-V3 region of 16S rDNA genes by 454 pyrosequencing. Those 16S rDNA sequences were classified into 16 phyla, 27 classes, 48 orders, 85 families, 126 genera, and 1141 species-level OTUs. Comparing periodontally healthy samples with periodontitis samples identified 20 health-associated and 15 periodontitis-associated OTUs. In the subjects with healthy periodontium, the abundances of three genera (Prevotella, Pseudomonas, and Tannerella) and nine OTUs were significantly different between diabetic patients and their non-diabetic counterparts. In the subjects carrying periodontitis, the abundances of three phyla (Actinobacteria, Proteobacteria, and Bacteriodetes), two genera (Actinomyces and Aggregatibacter), and six OTUs were also significantly different between diabetics and non-diabetics. Our results show that type 2 diabetes mellitus could alter the bacterial composition in the subgingival plaque.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0061516</identifier><identifier>PMID: 23613868</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Actinomyces ; Adult ; Aged ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Biology ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus, Type 2 - complications ; Diabetes Mellitus, Type 2 - microbiology ; Diabetics ; DNA sequencing ; DNA, Bacterial - genetics ; DNA, Ribosomal - genetics ; Education ; Gene sequencing ; Genera ; Gingiva - microbiology ; Gum disease ; Health risks ; High-Throughput Nucleotide Sequencing ; Hospitals ; Humans ; Investigations ; Laboratories ; Medical research ; Medicine ; Microbiota ; Microbiota (Symbiotic organisms) ; Microbiota - genetics ; Middle Aged ; Patients ; Periodontitis ; Periodontitis - microbiology ; Periodontium ; Porphyromonas gingivalis ; Risk factors ; rRNA 16S ; Science ; Sequence Analysis, DNA ; Studies ; Type 2 diabetes</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e61516-e61516</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Zhou, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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We investigated the effects of type 2 diabetes on the subgingival plaque bacterial composition by applying culture-independent 16S rDNA sequencing to periodontal bacteria isolated from four groups of volunteers: non-diabetic subjects without periodontitis, non-diabetic subjects with periodontitis, type 2 diabetic patients without periodontitis, and type 2 diabetic patients with periodontitis. A total of 71,373 high-quality sequences were produced from the V1-V3 region of 16S rDNA genes by 454 pyrosequencing. Those 16S rDNA sequences were classified into 16 phyla, 27 classes, 48 orders, 85 families, 126 genera, and 1141 species-level OTUs. Comparing periodontally healthy samples with periodontitis samples identified 20 health-associated and 15 periodontitis-associated OTUs. In the subjects with healthy periodontium, the abundances of three genera (Prevotella, Pseudomonas, and Tannerella) and nine OTUs were significantly different between diabetic patients and their non-diabetic counterparts. In the subjects carrying periodontitis, the abundances of three phyla (Actinobacteria, Proteobacteria, and Bacteriodetes), two genera (Actinomyces and Aggregatibacter), and six OTUs were also significantly different between diabetics and non-diabetics. Our results show that type 2 diabetes mellitus could alter the bacterial composition in the subgingival plaque.</description><subject>Actinomyces</subject><subject>Adult</subject><subject>Aged</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Biology</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Type 2 - complications</subject><subject>Diabetes Mellitus, Type 2 - microbiology</subject><subject>Diabetics</subject><subject>DNA sequencing</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Education</subject><subject>Gene sequencing</subject><subject>Genera</subject><subject>Gingiva - microbiology</subject><subject>Gum disease</subject><subject>Health risks</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Investigations</subject><subject>Laboratories</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Microbiota</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microbiota - genetics</subject><subject>Middle Aged</subject><subject>Patients</subject><subject>Periodontitis</subject><subject>Periodontitis - microbiology</subject><subject>Periodontium</subject><subject>Porphyromonas gingivalis</subject><subject>Risk factors</subject><subject>rRNA 16S</subject><subject>Science</subject><subject>Sequence Analysis, DNA</subject><subject>Studies</subject><subject>Type 2 diabetes</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBYQkJw2MVfcZIL0qp8rVRRiQJXy3EmiatsHGxnxZ754zjdtNpFPaAc4jjPvDN-PZMkzwleEpaRd9d2dL3qloPtYYmxICkRD5JTUjC6EBSzhwfrk-SJ99cYpywX4nFyQpkgcZmfJn_W_RZ8MI0KxvbI1ii0gKCuQYebr90AiKLKqBICeLSBrjNh9CjCfiwb0zdmqzo0dOrXCGhjtLOlsUGhcoda07SL0Do7Nu0wBkTEFXIfvq7QsHPWQwzodRR4mjyqVefh2fw-S358-vj9_Mvi4vLz-nx1sdCioGFBqGJZWlel4rpQWaXrgtd5SkUFaUa1AAAhqKBFxUWaE8ZKQYAWBGOgqcacnSUv97pDZ72c_fMykikWuKBZJNZ7orLqWg7ObJTbSauMvNmwrpHKBaM7kAoTXdWcc1VArAeXvKpSnReK1iyFDEet93O2sdxApaEPTnVHosd_etPKxm4lE4ymfCr3zSzgbLTKB7kxXkf_VQ92nOrmgpCc0yKir_5B7z_dTDUqHsD0tY159SQqVzzLSUZpPqVd3kPFp4J4u7HZahP3jwLeHgVEJsDv0KjRe7m--vb_7OXPY_b1AduC6kLrbTdOjeqPQb4HY-9576C-M5lgOc3KrRtymhU5z0oMe3F4QXdBt8PB_gJZrg_V</recordid><startdate>20130422</startdate><enddate>20130422</enddate><creator>Zhou, Mi</creator><creator>Rong, Ruichen</creator><creator>Munro, Daniel</creator><creator>Zhu, Chunxia</creator><creator>Gao, Xiang</creator><creator>Zhang, Qi</creator><creator>Dong, Qunfeng</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130422</creationdate><title>Investigation of the effect of type 2 diabetes mellitus on subgingival plaque microbiota by high-throughput 16S rDNA pyrosequencing</title><author>Zhou, Mi ; Rong, Ruichen ; Munro, Daniel ; Zhu, Chunxia ; Gao, Xiang ; Zhang, Qi ; Dong, Qunfeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-12a375fdba4c9a7dcf94f8526de572c6eee662629d4658133b61e29100e25c043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Actinomyces</topic><topic>Adult</topic><topic>Aged</topic><topic>Bacteria</topic><topic>Bacteria - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Mi</au><au>Rong, Ruichen</au><au>Munro, Daniel</au><au>Zhu, Chunxia</au><au>Gao, Xiang</au><au>Zhang, Qi</au><au>Dong, Qunfeng</au><au>Tunney, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of the effect of type 2 diabetes mellitus on subgingival plaque microbiota by high-throughput 16S rDNA pyrosequencing</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-04-22</date><risdate>2013</risdate><volume>8</volume><issue>4</issue><spage>e61516</spage><epage>e61516</epage><pages>e61516-e61516</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Diabetes mellitus is a major risk factor for chronic periodontitis. We investigated the effects of type 2 diabetes on the subgingival plaque bacterial composition by applying culture-independent 16S rDNA sequencing to periodontal bacteria isolated from four groups of volunteers: non-diabetic subjects without periodontitis, non-diabetic subjects with periodontitis, type 2 diabetic patients without periodontitis, and type 2 diabetic patients with periodontitis. A total of 71,373 high-quality sequences were produced from the V1-V3 region of 16S rDNA genes by 454 pyrosequencing. Those 16S rDNA sequences were classified into 16 phyla, 27 classes, 48 orders, 85 families, 126 genera, and 1141 species-level OTUs. Comparing periodontally healthy samples with periodontitis samples identified 20 health-associated and 15 periodontitis-associated OTUs. In the subjects with healthy periodontium, the abundances of three genera (Prevotella, Pseudomonas, and Tannerella) and nine OTUs were significantly different between diabetic patients and their non-diabetic counterparts. In the subjects carrying periodontitis, the abundances of three phyla (Actinobacteria, Proteobacteria, and Bacteriodetes), two genera (Actinomyces and Aggregatibacter), and six OTUs were also significantly different between diabetics and non-diabetics. Our results show that type 2 diabetes mellitus could alter the bacterial composition in the subgingival plaque.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23613868</pmid><doi>10.1371/journal.pone.0061516</doi><tpages>e61516</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actinomyces Adult Aged Bacteria Bacteria - classification Bacteria - genetics Biology Diabetes Diabetes mellitus Diabetes Mellitus, Type 2 - complications Diabetes Mellitus, Type 2 - microbiology Diabetics DNA sequencing DNA, Bacterial - genetics DNA, Ribosomal - genetics Education Gene sequencing Genera Gingiva - microbiology Gum disease Health risks High-Throughput Nucleotide Sequencing Hospitals Humans Investigations Laboratories Medical research Medicine Microbiota Microbiota (Symbiotic organisms) Microbiota - genetics Middle Aged Patients Periodontitis Periodontitis - microbiology Periodontium Porphyromonas gingivalis Risk factors rRNA 16S Science Sequence Analysis, DNA Studies Type 2 diabetes |
| title | Investigation of the effect of type 2 diabetes mellitus on subgingival plaque microbiota by high-throughput 16S rDNA pyrosequencing |
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