Functional specificity of sex pheromone receptors in the cotton bollworm Helicoverpa armigera
Male moths can accurately perceive the sex pheromone emitted from conspecific females by their highly accurate and specific olfactory sensory system. Pheromone receptors are of special importance in moth pheromone reception because of their central role in chemosensory signal transduction processes...
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| creator | Liu, Yang Liu, Chengcheng Lin, Kejian Wang, Guirong |
| description | Male moths can accurately perceive the sex pheromone emitted from conspecific females by their highly accurate and specific olfactory sensory system. Pheromone receptors are of special importance in moth pheromone reception because of their central role in chemosensory signal transduction processes that occur in olfactory receptor neurons in the male antennae. There are a number of pheromone receptor genes have been cloned, however, only a few have been functionally characterized. Here we cloned six full-length pheromone receptor genes from Helicoverpa armigera male antennae. Real-time PCR showing all genes exhibited male-biased expression in adult antennae. Functional analyses of the six pheromone receptor genes were then conducted in the heterologous expression system of Xenopus oocytes. HarmOR13 was found to be a specific receptor for the major sex pheromone component Z11-16:Ald. HarmOR6 was equally tuned to both of Z9-16: Ald and Z9-14: Ald. HarmOR16 was sensitively tuned to Z11-16: OH. HarmOR11, HarmOR14 and HarmOR15 failed to respond to the tested candidate pheromone compounds. Our experiments elucidated the functions of some pheromone receptor genes of H. armigera. These advances may provide remarkable evidence for intraspecific mating choice and speciation extension in moths at molecular level. |
| doi_str_mv | 10.1371/journal.pone.0062094 |
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Pheromone receptors are of special importance in moth pheromone reception because of their central role in chemosensory signal transduction processes that occur in olfactory receptor neurons in the male antennae. There are a number of pheromone receptor genes have been cloned, however, only a few have been functionally characterized. Here we cloned six full-length pheromone receptor genes from Helicoverpa armigera male antennae. Real-time PCR showing all genes exhibited male-biased expression in adult antennae. Functional analyses of the six pheromone receptor genes were then conducted in the heterologous expression system of Xenopus oocytes. HarmOR13 was found to be a specific receptor for the major sex pheromone component Z11-16:Ald. HarmOR6 was equally tuned to both of Z9-16: Ald and Z9-14: Ald. HarmOR16 was sensitively tuned to Z11-16: OH. HarmOR11, HarmOR14 and HarmOR15 failed to respond to the tested candidate pheromone compounds. Our experiments elucidated the functions of some pheromone receptor genes of H. armigera. These advances may provide remarkable evidence for intraspecific mating choice and speciation extension in moths at molecular level.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0062094</identifier><identifier>PMID: 23614018</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Amino Acid Sequence ; Analysis ; Animal behavior ; Animals ; Anopheles gambiae ; Antennae ; Biology ; Butterflies & moths ; Chemoreception ; Cloning, Molecular ; Cotton ; Drosophila ; Electrophysiological Phenomena ; Female ; Females ; Gene expression ; Genes ; Helicoverpa armigera ; Heliothis ; Heliothis virescens ; Insect Proteins - chemistry ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Lepidoptera - genetics ; Lepidoptera - metabolism ; Lepidoptera - physiology ; Male ; Mating ; Molecular Sequence Data ; Moths ; Neurophysiology ; Odorant receptors ; Olfactory receptor neurons ; Oocytes ; Organ Specificity ; Ostrinia ; Pheromone receptors ; Pheromones ; Plant diseases ; Receptors ; Receptors, Pheromone - chemistry ; Receptors, Pheromone - genetics ; Receptors, Pheromone - metabolism ; Sequence Analysis ; Sequence Homology, Nucleic Acid ; Sex ; Sex Attractants - metabolism ; Sex Characteristics ; Sex pheromone ; Signal processing ; Speciation ; Substrate Specificity ; Transduction ; Xenopus</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e62094</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Pheromone receptors are of special importance in moth pheromone reception because of their central role in chemosensory signal transduction processes that occur in olfactory receptor neurons in the male antennae. There are a number of pheromone receptor genes have been cloned, however, only a few have been functionally characterized. Here we cloned six full-length pheromone receptor genes from Helicoverpa armigera male antennae. Real-time PCR showing all genes exhibited male-biased expression in adult antennae. Functional analyses of the six pheromone receptor genes were then conducted in the heterologous expression system of Xenopus oocytes. HarmOR13 was found to be a specific receptor for the major sex pheromone component Z11-16:Ald. HarmOR6 was equally tuned to both of Z9-16: Ald and Z9-14: Ald. HarmOR16 was sensitively tuned to Z11-16: OH. HarmOR11, HarmOR14 and HarmOR15 failed to respond to the tested candidate pheromone compounds. Our experiments elucidated the functions of some pheromone receptor genes of H. armigera. These advances may provide remarkable evidence for intraspecific mating choice and speciation extension in moths at molecular level.</description><subject>Agriculture</subject><subject>Amino Acid Sequence</subject><subject>Analysis</subject><subject>Animal behavior</subject><subject>Animals</subject><subject>Anopheles gambiae</subject><subject>Antennae</subject><subject>Biology</subject><subject>Butterflies & moths</subject><subject>Chemoreception</subject><subject>Cloning, Molecular</subject><subject>Cotton</subject><subject>Drosophila</subject><subject>Electrophysiological Phenomena</subject><subject>Female</subject><subject>Females</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Helicoverpa armigera</subject><subject>Heliothis</subject><subject>Heliothis virescens</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Lepidoptera - 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processing</subject><subject>Speciation</subject><subject>Substrate 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specificity of sex pheromone receptors in the cotton bollworm Helicoverpa armigera</title><author>Liu, Yang ; Liu, Chengcheng ; Lin, Kejian ; Wang, Guirong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-102b4a3fdd9a1fcc56477c81b1d73f6f360d8c30a566ebd03008260d9d457b913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Agriculture</topic><topic>Amino Acid Sequence</topic><topic>Analysis</topic><topic>Animal behavior</topic><topic>Animals</topic><topic>Anopheles gambiae</topic><topic>Antennae</topic><topic>Biology</topic><topic>Butterflies & moths</topic><topic>Chemoreception</topic><topic>Cloning, Molecular</topic><topic>Cotton</topic><topic>Drosophila</topic><topic>Electrophysiological Phenomena</topic><topic>Female</topic><topic>Females</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Helicoverpa armigera</topic><topic>Heliothis</topic><topic>Heliothis virescens</topic><topic>Insect Proteins - chemistry</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Lepidoptera - genetics</topic><topic>Lepidoptera - metabolism</topic><topic>Lepidoptera - physiology</topic><topic>Male</topic><topic>Mating</topic><topic>Molecular Sequence Data</topic><topic>Moths</topic><topic>Neurophysiology</topic><topic>Odorant receptors</topic><topic>Olfactory receptor neurons</topic><topic>Oocytes</topic><topic>Organ Specificity</topic><topic>Ostrinia</topic><topic>Pheromone receptors</topic><topic>Pheromones</topic><topic>Plant diseases</topic><topic>Receptors</topic><topic>Receptors, Pheromone - chemistry</topic><topic>Receptors, Pheromone - genetics</topic><topic>Receptors, Pheromone - metabolism</topic><topic>Sequence Analysis</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Sex</topic><topic>Sex Attractants - metabolism</topic><topic>Sex 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Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yang</au><au>Liu, Chengcheng</au><au>Lin, Kejian</au><au>Wang, Guirong</au><au>Newcomb, Richard David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional specificity of sex pheromone receptors in the cotton bollworm Helicoverpa armigera</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-04-15</date><risdate>2013</risdate><volume>8</volume><issue>4</issue><spage>e62094</spage><pages>e62094-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Male moths can accurately perceive the sex pheromone emitted from conspecific females by their highly accurate and specific olfactory sensory system. Pheromone receptors are of special importance in moth pheromone reception because of their central role in chemosensory signal transduction processes that occur in olfactory receptor neurons in the male antennae. There are a number of pheromone receptor genes have been cloned, however, only a few have been functionally characterized. Here we cloned six full-length pheromone receptor genes from Helicoverpa armigera male antennae. Real-time PCR showing all genes exhibited male-biased expression in adult antennae. Functional analyses of the six pheromone receptor genes were then conducted in the heterologous expression system of Xenopus oocytes. HarmOR13 was found to be a specific receptor for the major sex pheromone component Z11-16:Ald. HarmOR6 was equally tuned to both of Z9-16: Ald and Z9-14: Ald. HarmOR16 was sensitively tuned to Z11-16: OH. HarmOR11, HarmOR14 and HarmOR15 failed to respond to the tested candidate pheromone compounds. Our experiments elucidated the functions of some pheromone receptor genes of H. armigera. These advances may provide remarkable evidence for intraspecific mating choice and speciation extension in moths at molecular level.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23614018</pmid><doi>10.1371/journal.pone.0062094</doi><tpages>e62094</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agriculture Amino Acid Sequence Analysis Animal behavior Animals Anopheles gambiae Antennae Biology Butterflies & moths Chemoreception Cloning, Molecular Cotton Drosophila Electrophysiological Phenomena Female Females Gene expression Genes Helicoverpa armigera Heliothis Heliothis virescens Insect Proteins - chemistry Insect Proteins - genetics Insect Proteins - metabolism Lepidoptera - genetics Lepidoptera - metabolism Lepidoptera - physiology Male Mating Molecular Sequence Data Moths Neurophysiology Odorant receptors Olfactory receptor neurons Oocytes Organ Specificity Ostrinia Pheromone receptors Pheromones Plant diseases Receptors Receptors, Pheromone - chemistry Receptors, Pheromone - genetics Receptors, Pheromone - metabolism Sequence Analysis Sequence Homology, Nucleic Acid Sex Sex Attractants - metabolism Sex Characteristics Sex pheromone Signal processing Speciation Substrate Specificity Transduction Xenopus |
| title | Functional specificity of sex pheromone receptors in the cotton bollworm Helicoverpa armigera |
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