Genetic diversity and distribution of the ciguatera-causing dinoflagellate Gambierdiscus spp. (Dinophyceae) in coastal areas of Japan
The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in subtropical are...
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| Veröffentlicht in: | PloS one 2013-04, Vol.8 (4), p.e60882 |
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| creator | Nishimura, Tomohiro Sato, Shinya Tawong, Wittaya Sakanari, Hiroshi Uehara, Keita Shah, Md Mahfuzur Rahman Suda, Shoichiro Yasumoto, Takeshi Taira, Yohsuke Yamaguchi, Haruo Adachi, Masao |
| description | The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in subtropical areas. A previous phylogeographic study of Japanese Gambierdiscus revealed the existence of two distinct phylotypes: Gambierdiscus sp. type 1 from subtropical and Gambierdiscus sp. type 2 from temperate areas. However, details of the genetic diversity and distribution for Japanese Gambierdiscus are still unclear, because a comprehensive investigation has not been conducted yet.
A total of 248 strains were examined from samples mainly collected from western and southern coastal areas of Japan during 2006-2011. The SSU rDNA, the LSU rDNA D8-D10 and the ITS region were selected as genetic markers and phylogenetic analyses were conducted. The genetic diversity of Japanese Gambierdiscus was high since five species/phylotypes were detected: including two reported phylotypes (Gambierdiscus sp. type 1 and Gambierdiscus sp. type 2), two species of Gambierdiscus (G. australes and G. cf. yasumotoi) and a hitherto unreported phylotype Gambierdiscus sp. type 3. The distributions of type 3 and G. cf. yasumotoi were restricted to the temperate and the subtropical area, respectively. On the other hand, type 1, type 2 and G. australes occurred from the subtropical to the temperate area, with a tendency that type 1 and G. australes were dominant in the subtropical area, whereas type 2 was dominant in the temperate area. By using mouse bioassay, type 1, type 3 and G. australes exhibited mouse toxicities.
This study revealed a surprising diversity of Japanese Gambierdiscus and the distribution of five species/phylotypes displayed clear geographical patterns in Japanese coastal areas. The SSU rDNA and the LSU rDNA D8-D10 as genetic markers are recommended for further use. |
| doi_str_mv | 10.1371/journal.pone.0060882 |
| format | Article |
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A total of 248 strains were examined from samples mainly collected from western and southern coastal areas of Japan during 2006-2011. The SSU rDNA, the LSU rDNA D8-D10 and the ITS region were selected as genetic markers and phylogenetic analyses were conducted. The genetic diversity of Japanese Gambierdiscus was high since five species/phylotypes were detected: including two reported phylotypes (Gambierdiscus sp. type 1 and Gambierdiscus sp. type 2), two species of Gambierdiscus (G. australes and G. cf. yasumotoi) and a hitherto unreported phylotype Gambierdiscus sp. type 3. The distributions of type 3 and G. cf. yasumotoi were restricted to the temperate and the subtropical area, respectively. On the other hand, type 1, type 2 and G. australes occurred from the subtropical to the temperate area, with a tendency that type 1 and G. australes were dominant in the subtropical area, whereas type 2 was dominant in the temperate area. By using mouse bioassay, type 1, type 3 and G. australes exhibited mouse toxicities.
This study revealed a surprising diversity of Japanese Gambierdiscus and the distribution of five species/phylotypes displayed clear geographical patterns in Japanese coastal areas. The SSU rDNA and the LSU rDNA D8-D10 as genetic markers are recommended for further use.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0060882</identifier><identifier>PMID: 23593339</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agriculture ; Animals ; Bayes Theorem ; Bioassays ; Biodiversity ; Bioinformatics ; Biological Assay ; Biology ; Ciguatera ; Ciguatoxins - metabolism ; Ciguatoxins - toxicity ; Coastal zone ; Coasts ; Demography ; Dinoflagellida - genetics ; Dinoflagellida - metabolism ; Dinophyceae ; DNA, Ribosomal - genetics ; Earth Sciences ; Evolution, Molecular ; Financial planners ; Fish ; Gambierdiscus ; Gambierdiscus toxicus ; Genetic diversity ; Genetic Markers ; Genetic research ; Genetic Variation ; Illnesses ; Japan ; Marine toxins ; Markers ; Medicine ; Mice ; Microorganisms ; Models, Genetic ; Morphology ; Pfiesteria ; Phylogenetics ; Phylogeny ; Phylogeography ; Poisoning ; Science ; Seafood ; Species ; Species diversity ; Toxicity ; Toxins ; Veterinary Science</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e60882</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Nishimura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Nishimura et al 2013 Nishimura et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-7a6cef7a1a77f4de64b6043f45a7bb92c7cc9e238bb5c16182b5b14306e769f03</citedby><cites>FETCH-LOGICAL-c692t-7a6cef7a1a77f4de64b6043f45a7bb92c7cc9e238bb5c16182b5b14306e769f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623954/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3623954/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23871,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23593339$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Neilan, Brett</contributor><creatorcontrib>Nishimura, Tomohiro</creatorcontrib><creatorcontrib>Sato, Shinya</creatorcontrib><creatorcontrib>Tawong, Wittaya</creatorcontrib><creatorcontrib>Sakanari, Hiroshi</creatorcontrib><creatorcontrib>Uehara, Keita</creatorcontrib><creatorcontrib>Shah, Md Mahfuzur Rahman</creatorcontrib><creatorcontrib>Suda, Shoichiro</creatorcontrib><creatorcontrib>Yasumoto, Takeshi</creatorcontrib><creatorcontrib>Taira, Yohsuke</creatorcontrib><creatorcontrib>Yamaguchi, Haruo</creatorcontrib><creatorcontrib>Adachi, Masao</creatorcontrib><title>Genetic diversity and distribution of the ciguatera-causing dinoflagellate Gambierdiscus spp. (Dinophyceae) in coastal areas of Japan</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in subtropical areas. A previous phylogeographic study of Japanese Gambierdiscus revealed the existence of two distinct phylotypes: Gambierdiscus sp. type 1 from subtropical and Gambierdiscus sp. type 2 from temperate areas. However, details of the genetic diversity and distribution for Japanese Gambierdiscus are still unclear, because a comprehensive investigation has not been conducted yet.
A total of 248 strains were examined from samples mainly collected from western and southern coastal areas of Japan during 2006-2011. The SSU rDNA, the LSU rDNA D8-D10 and the ITS region were selected as genetic markers and phylogenetic analyses were conducted. The genetic diversity of Japanese Gambierdiscus was high since five species/phylotypes were detected: including two reported phylotypes (Gambierdiscus sp. type 1 and Gambierdiscus sp. type 2), two species of Gambierdiscus (G. australes and G. cf. yasumotoi) and a hitherto unreported phylotype Gambierdiscus sp. type 3. The distributions of type 3 and G. cf. yasumotoi were restricted to the temperate and the subtropical area, respectively. On the other hand, type 1, type 2 and G. australes occurred from the subtropical to the temperate area, with a tendency that type 1 and G. australes were dominant in the subtropical area, whereas type 2 was dominant in the temperate area. By using mouse bioassay, type 1, type 3 and G. australes exhibited mouse toxicities.
This study revealed a surprising diversity of Japanese Gambierdiscus and the distribution of five species/phylotypes displayed clear geographical patterns in Japanese coastal areas. The SSU rDNA and the LSU rDNA D8-D10 as genetic markers are recommended for further use.</description><subject>Agriculture</subject><subject>Animals</subject><subject>Bayes Theorem</subject><subject>Bioassays</subject><subject>Biodiversity</subject><subject>Bioinformatics</subject><subject>Biological Assay</subject><subject>Biology</subject><subject>Ciguatera</subject><subject>Ciguatoxins - metabolism</subject><subject>Ciguatoxins - toxicity</subject><subject>Coastal zone</subject><subject>Coasts</subject><subject>Demography</subject><subject>Dinoflagellida - genetics</subject><subject>Dinoflagellida - metabolism</subject><subject>Dinophyceae</subject><subject>DNA, Ribosomal - genetics</subject><subject>Earth Sciences</subject><subject>Evolution, Molecular</subject><subject>Financial planners</subject><subject>Fish</subject><subject>Gambierdiscus</subject><subject>Gambierdiscus toxicus</subject><subject>Genetic diversity</subject><subject>Genetic Markers</subject><subject>Genetic research</subject><subject>Genetic Variation</subject><subject>Illnesses</subject><subject>Japan</subject><subject>Marine toxins</subject><subject>Markers</subject><subject>Medicine</subject><subject>Mice</subject><subject>Microorganisms</subject><subject>Models, Genetic</subject><subject>Morphology</subject><subject>Pfiesteria</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Phylogeography</subject><subject>Poisoning</subject><subject>Science</subject><subject>Seafood</subject><subject>Species</subject><subject>Species diversity</subject><subject>Toxicity</subject><subject>Toxins</subject><subject>Veterinary 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Brett</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity and distribution of the ciguatera-causing dinoflagellate Gambierdiscus spp. (Dinophyceae) in coastal areas of Japan</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-04-11</date><risdate>2013</risdate><volume>8</volume><issue>4</issue><spage>e60882</spage><pages>e60882-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in subtropical areas. A previous phylogeographic study of Japanese Gambierdiscus revealed the existence of two distinct phylotypes: Gambierdiscus sp. type 1 from subtropical and Gambierdiscus sp. type 2 from temperate areas. However, details of the genetic diversity and distribution for Japanese Gambierdiscus are still unclear, because a comprehensive investigation has not been conducted yet.
A total of 248 strains were examined from samples mainly collected from western and southern coastal areas of Japan during 2006-2011. The SSU rDNA, the LSU rDNA D8-D10 and the ITS region were selected as genetic markers and phylogenetic analyses were conducted. The genetic diversity of Japanese Gambierdiscus was high since five species/phylotypes were detected: including two reported phylotypes (Gambierdiscus sp. type 1 and Gambierdiscus sp. type 2), two species of Gambierdiscus (G. australes and G. cf. yasumotoi) and a hitherto unreported phylotype Gambierdiscus sp. type 3. The distributions of type 3 and G. cf. yasumotoi were restricted to the temperate and the subtropical area, respectively. On the other hand, type 1, type 2 and G. australes occurred from the subtropical to the temperate area, with a tendency that type 1 and G. australes were dominant in the subtropical area, whereas type 2 was dominant in the temperate area. By using mouse bioassay, type 1, type 3 and G. australes exhibited mouse toxicities.
This study revealed a surprising diversity of Japanese Gambierdiscus and the distribution of five species/phylotypes displayed clear geographical patterns in Japanese coastal areas. The SSU rDNA and the LSU rDNA D8-D10 as genetic markers are recommended for further use.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23593339</pmid><doi>10.1371/journal.pone.0060882</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-04, Vol.8 (4), p.e60882 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1335051439 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agriculture Animals Bayes Theorem Bioassays Biodiversity Bioinformatics Biological Assay Biology Ciguatera Ciguatoxins - metabolism Ciguatoxins - toxicity Coastal zone Coasts Demography Dinoflagellida - genetics Dinoflagellida - metabolism Dinophyceae DNA, Ribosomal - genetics Earth Sciences Evolution, Molecular Financial planners Fish Gambierdiscus Gambierdiscus toxicus Genetic diversity Genetic Markers Genetic research Genetic Variation Illnesses Japan Marine toxins Markers Medicine Mice Microorganisms Models, Genetic Morphology Pfiesteria Phylogenetics Phylogeny Phylogeography Poisoning Science Seafood Species Species diversity Toxicity Toxins Veterinary Science |
| title | Genetic diversity and distribution of the ciguatera-causing dinoflagellate Gambierdiscus spp. (Dinophyceae) in coastal areas of Japan |
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