Overexpression of peanut diacylglycerol acyltransferase 2 in Escherichia coli

Diacylglycerol acyltransferase (DGAT) is the rate-limiting enzyme in triacylglycerol biosynthesis in eukaryotic organisms. Triacylglycerols are important energy-storage oils in plants such as peanuts, soybeans and rape. In this study, Arachis hypogaea type 2 DGAT (AhDGAT2) genes were cloned from the...

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Veröffentlicht in:	PloS one 2013-04, Vol.8 (4), p.e61363-e61363
Hauptverfasser:	Peng, Zhenying, Li, Lan, Yang, Lianqun, Zhang, Bin, Chen, Gao, Bi, Yuping
Format:	Artikel
Sprache:	eng
Schlagworte:	Acyltransferase Amino acids Analysis Analysis of Variance Arachis - enzymology Arachis hypogaea Bacteria Biology Biosynthesis Biotechnology - methods Cell growth Cloning, Molecular Cluster Analysis Composition effects Computational Biology Cytoplasm Diacylglycerol O-acyltransferase Diacylglycerol O-Acyltransferase - genetics Diacylglycerol O-Acyltransferase - metabolism Diglycerides DNA Primers - genetics E coli Energy storage Engineering Enzymes Escherichia coli Fatty acids Fatty Acids - metabolism Genes Glutathione Glutathione transferase Glutathione Transferase - metabolism Growth rate Homology Inclusion bodies Isoenzymes Laboratories Life sciences Morphology Nut industry Nuts Peanuts Phylogeny Physiological aspects Physiology Plants (botany) Proteins Recombinant Fusion Proteins - metabolism Seeds Soybeans Transformed cells Triglycerides Triglycerides - biosynthesis Vectors Vegetable oils
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