Experimental infection of dogs with Leishmania and saliva as a model to study Canine Visceral Leishmaniasis
Canine Visceral Leishmaniasis (CVL) is a zoonotic disease caused by Leishmania infantum, transmitted by the bite of Lutzomyia longipalpis sand flies. Dogs are the main domestic reservoir of the parasite. The establishment of an experimental model that partially reproduces natural infection in dogs i...
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| Veröffentlicht in: | PloS one 2013-04, Vol.8 (4), p.e60535-e60535 |
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| creator | Costa, Dirceu Joaquim Carvalho, Rayssa M de Araujo Abbehusen, Melissa Teixeira, Clarissa Pitombo, Maiana Trigo, Joelma Nascimento, Flávia Amorim, Lucilene Abreu-Silva, Ana Lucia do Socorro Pires Cruz, Maria Miranda, José Carlos Fukutani, Kyoshi de Oliveira, Camila I Barral, Aldina Barral-Netto, Manoel Brodskyn, Cláudia |
| description | Canine Visceral Leishmaniasis (CVL) is a zoonotic disease caused by Leishmania infantum, transmitted by the bite of Lutzomyia longipalpis sand flies. Dogs are the main domestic reservoir of the parasite. The establishment of an experimental model that partially reproduces natural infection in dogs is very important to test vaccine candidates, mainly regarding those that use salivary proteins from the vector and new therapeutical approaches.
In this report, we describe an experimental infection in dogs, using intradermal injection of Leishmania infantum plus salivary gland homogenate (SGH) of Lutzomyia longipalpis. Thirty-five dogs were infected with 1×10(7) parasites combined with five pairs of Lutzomyia longipalpis salivary glands and followed for 450 days after infection and clinical, immunological and parasitological parameters were evaluated. Two hundred and ten days after infection we observed that 31,4% of dogs did not display detectable levels of anti-Leishmania antibodies but all presented different numbers of parasites in the lymph nodes. Animals with a positive xenodiagnosis had at least 3,35×10(5) parasites in their lymph nodes. An increase of IFN-γ and IL-10 levels was detected during infection. Twenty two percent of dogs developed symptoms of CVL during infection.
The infection model described here shows some degree of similarity when compared with naturally infected dogs opening new perspectives for the study of CVL using an experimental model that employs the combination of parasites and sand fly saliva both present during natural transmission. |
| doi_str_mv | 10.1371/journal.pone.0060535 |
| format | Article |
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In this report, we describe an experimental infection in dogs, using intradermal injection of Leishmania infantum plus salivary gland homogenate (SGH) of Lutzomyia longipalpis. Thirty-five dogs were infected with 1×10(7) parasites combined with five pairs of Lutzomyia longipalpis salivary glands and followed for 450 days after infection and clinical, immunological and parasitological parameters were evaluated. Two hundred and ten days after infection we observed that 31,4% of dogs did not display detectable levels of anti-Leishmania antibodies but all presented different numbers of parasites in the lymph nodes. Animals with a positive xenodiagnosis had at least 3,35×10(5) parasites in their lymph nodes. An increase of IFN-γ and IL-10 levels was detected during infection. Twenty two percent of dogs developed symptoms of CVL during infection.
The infection model described here shows some degree of similarity when compared with naturally infected dogs opening new perspectives for the study of CVL using an experimental model that employs the combination of parasites and sand fly saliva both present during natural transmission.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0060535</identifier><identifier>PMID: 23577121</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antibodies ; Antibodies, Protozoan - blood ; Biology ; Cytokines - blood ; Disease Models, Animal ; Disease transmission ; Dog Diseases - blood ; Dog Diseases - parasitology ; Dog Diseases - transmission ; Dogs ; Experimental infection ; Female ; Glands ; Health aspects ; Immunoglobulin G - blood ; Immunology ; Infection ; Interferon ; Interleukin 10 ; Leishmania ; Leishmania infantum ; Leishmania infantum - immunology ; Leishmania infantum - physiology ; Leishmaniasis, Visceral - blood ; Leishmaniasis, Visceral - parasitology ; Leishmaniasis, Visceral - transmission ; Leishmaniasis, Visceral - veterinary ; Lutzomyia longipalpis ; Lymph nodes ; Medical research ; Parasite Load ; Parasites ; Parasitic diseases ; Proteins ; Psychodidae - parasitology ; Saliva ; Saliva - parasitology ; Salivary gland ; Salivary glands ; Salivary Glands - parasitology ; Vaccines ; Vector-borne diseases ; Veterinary Science ; Visceral leishmaniasis ; Xenodiagnosis ; Zoonoses ; γ-Interferon</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e60535-e60535</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Costa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Costa et al 2013 Costa et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-3083f20a72e80ce5964bce45add8bd67977f2c28c4f95135571306288ac074623</citedby><cites>FETCH-LOGICAL-c692t-3083f20a72e80ce5964bce45add8bd67977f2c28c4f95135571306288ac074623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618420/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3618420/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23577121$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Costa, Dirceu Joaquim</creatorcontrib><creatorcontrib>Carvalho, Rayssa M de Araujo</creatorcontrib><creatorcontrib>Abbehusen, Melissa</creatorcontrib><creatorcontrib>Teixeira, Clarissa</creatorcontrib><creatorcontrib>Pitombo, Maiana</creatorcontrib><creatorcontrib>Trigo, Joelma</creatorcontrib><creatorcontrib>Nascimento, Flávia</creatorcontrib><creatorcontrib>Amorim, Lucilene</creatorcontrib><creatorcontrib>Abreu-Silva, Ana Lucia</creatorcontrib><creatorcontrib>do Socorro Pires Cruz, Maria</creatorcontrib><creatorcontrib>Miranda, José Carlos</creatorcontrib><creatorcontrib>Fukutani, Kyoshi</creatorcontrib><creatorcontrib>de Oliveira, Camila I</creatorcontrib><creatorcontrib>Barral, Aldina</creatorcontrib><creatorcontrib>Barral-Netto, Manoel</creatorcontrib><creatorcontrib>Brodskyn, Cláudia</creatorcontrib><title>Experimental infection of dogs with Leishmania and saliva as a model to study Canine Visceral Leishmaniasis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Canine Visceral Leishmaniasis (CVL) is a zoonotic disease caused by Leishmania infantum, transmitted by the bite of Lutzomyia longipalpis sand flies. Dogs are the main domestic reservoir of the parasite. The establishment of an experimental model that partially reproduces natural infection in dogs is very important to test vaccine candidates, mainly regarding those that use salivary proteins from the vector and new therapeutical approaches.
In this report, we describe an experimental infection in dogs, using intradermal injection of Leishmania infantum plus salivary gland homogenate (SGH) of Lutzomyia longipalpis. Thirty-five dogs were infected with 1×10(7) parasites combined with five pairs of Lutzomyia longipalpis salivary glands and followed for 450 days after infection and clinical, immunological and parasitological parameters were evaluated. Two hundred and ten days after infection we observed that 31,4% of dogs did not display detectable levels of anti-Leishmania antibodies but all presented different numbers of parasites in the lymph nodes. Animals with a positive xenodiagnosis had at least 3,35×10(5) parasites in their lymph nodes. An increase of IFN-γ and IL-10 levels was detected during infection. Twenty two percent of dogs developed symptoms of CVL during infection.
The infection model described here shows some degree of similarity when compared with naturally infected dogs opening new perspectives for the study of CVL using an experimental model that employs the combination of parasites and sand fly saliva both present during natural transmission.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Protozoan - blood</subject><subject>Biology</subject><subject>Cytokines - blood</subject><subject>Disease Models, Animal</subject><subject>Disease transmission</subject><subject>Dog Diseases - blood</subject><subject>Dog Diseases - parasitology</subject><subject>Dog Diseases - transmission</subject><subject>Dogs</subject><subject>Experimental infection</subject><subject>Female</subject><subject>Glands</subject><subject>Health aspects</subject><subject>Immunoglobulin G - blood</subject><subject>Immunology</subject><subject>Infection</subject><subject>Interferon</subject><subject>Interleukin 10</subject><subject>Leishmania</subject><subject>Leishmania infantum</subject><subject>Leishmania infantum - immunology</subject><subject>Leishmania infantum - physiology</subject><subject>Leishmaniasis, Visceral - blood</subject><subject>Leishmaniasis, Visceral - parasitology</subject><subject>Leishmaniasis, Visceral - transmission</subject><subject>Leishmaniasis, Visceral - veterinary</subject><subject>Lutzomyia longipalpis</subject><subject>Lymph nodes</subject><subject>Medical research</subject><subject>Parasite Load</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Proteins</subject><subject>Psychodidae - parasitology</subject><subject>Saliva</subject><subject>Saliva - parasitology</subject><subject>Salivary gland</subject><subject>Salivary glands</subject><subject>Salivary Glands - parasitology</subject><subject>Vaccines</subject><subject>Vector-borne diseases</subject><subject>Veterinary Science</subject><subject>Visceral 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Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Costa, Dirceu Joaquim</au><au>Carvalho, Rayssa M de Araujo</au><au>Abbehusen, Melissa</au><au>Teixeira, Clarissa</au><au>Pitombo, Maiana</au><au>Trigo, Joelma</au><au>Nascimento, Flávia</au><au>Amorim, Lucilene</au><au>Abreu-Silva, Ana Lucia</au><au>do Socorro Pires Cruz, Maria</au><au>Miranda, José Carlos</au><au>Fukutani, Kyoshi</au><au>de Oliveira, Camila I</au><au>Barral, Aldina</au><au>Barral-Netto, Manoel</au><au>Brodskyn, Cláudia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Experimental infection of dogs with Leishmania and saliva as a model to study Canine Visceral Leishmaniasis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-04-05</date><risdate>2013</risdate><volume>8</volume><issue>4</issue><spage>e60535</spage><epage>e60535</epage><pages>e60535-e60535</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Canine Visceral Leishmaniasis (CVL) is a zoonotic disease caused by Leishmania infantum, transmitted by the bite of Lutzomyia longipalpis sand flies. Dogs are the main domestic reservoir of the parasite. The establishment of an experimental model that partially reproduces natural infection in dogs is very important to test vaccine candidates, mainly regarding those that use salivary proteins from the vector and new therapeutical approaches.
In this report, we describe an experimental infection in dogs, using intradermal injection of Leishmania infantum plus salivary gland homogenate (SGH) of Lutzomyia longipalpis. Thirty-five dogs were infected with 1×10(7) parasites combined with five pairs of Lutzomyia longipalpis salivary glands and followed for 450 days after infection and clinical, immunological and parasitological parameters were evaluated. Two hundred and ten days after infection we observed that 31,4% of dogs did not display detectable levels of anti-Leishmania antibodies but all presented different numbers of parasites in the lymph nodes. Animals with a positive xenodiagnosis had at least 3,35×10(5) parasites in their lymph nodes. An increase of IFN-γ and IL-10 levels was detected during infection. Twenty two percent of dogs developed symptoms of CVL during infection.
The infection model described here shows some degree of similarity when compared with naturally infected dogs opening new perspectives for the study of CVL using an experimental model that employs the combination of parasites and sand fly saliva both present during natural transmission.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23577121</pmid><doi>10.1371/journal.pone.0060535</doi><tpages>e60535</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-04, Vol.8 (4), p.e60535-e60535 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1330914207 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Animals Antibodies Antibodies, Protozoan - blood Biology Cytokines - blood Disease Models, Animal Disease transmission Dog Diseases - blood Dog Diseases - parasitology Dog Diseases - transmission Dogs Experimental infection Female Glands Health aspects Immunoglobulin G - blood Immunology Infection Interferon Interleukin 10 Leishmania Leishmania infantum Leishmania infantum - immunology Leishmania infantum - physiology Leishmaniasis, Visceral - blood Leishmaniasis, Visceral - parasitology Leishmaniasis, Visceral - transmission Leishmaniasis, Visceral - veterinary Lutzomyia longipalpis Lymph nodes Medical research Parasite Load Parasites Parasitic diseases Proteins Psychodidae - parasitology Saliva Saliva - parasitology Salivary gland Salivary glands Salivary Glands - parasitology Vaccines Vector-borne diseases Veterinary Science Visceral leishmaniasis Xenodiagnosis Zoonoses γ-Interferon |
| title | Experimental infection of dogs with Leishmania and saliva as a model to study Canine Visceral Leishmaniasis |
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