Decreased expression of Beclin 1 correlates closely with Bcl-xL expression and poor prognosis of ovarian carcinoma
It has been suggested that autophagy-related Beclin 1 plays a critical role in the regulation of tumor development and/or progression, but its prognostic significance and relationship with Bcl-xL expression in ovarian carcinoma are unclear. In the present study, the methods of Western blotting and i...
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| creator | Lin, Huan-Xin Qiu, Hui-Juan Zeng, Fei Rao, Hui-Lan Yang, Guo-Fen Kung, Hsiang-Fu Zhu, Xiao-Feng Zeng, Yi-Xin Cai, Mu-Yan Xie, Dan |
| description | It has been suggested that autophagy-related Beclin 1 plays a critical role in the regulation of tumor development and/or progression, but its prognostic significance and relationship with Bcl-xL expression in ovarian carcinoma are unclear.
In the present study, the methods of Western blotting and immunohistochemistry (IHC) were utilized to investigate the expression status of Beclin 1 and Bcl-xL in fresh ovarian tissues and paraffin-embedded epithelial ovarian tumor tissues. Decreased expression of Beclin 1 was examined by IHC in 8.3% of normal ovaries, in 15.4% of cystadenomas, in 20.0% of borderline tumors, and in 55.6% of ovarian carcinomas, respectively. In ovarian carcinomas, decreased expression of Beclin 1 was correlated closely with ascending histological grade, later pT/pN/pM status and/or advanced clinical stage (P |
| doi_str_mv | 10.1371/journal.pone.0060516 |
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In the present study, the methods of Western blotting and immunohistochemistry (IHC) were utilized to investigate the expression status of Beclin 1 and Bcl-xL in fresh ovarian tissues and paraffin-embedded epithelial ovarian tumor tissues. Decreased expression of Beclin 1 was examined by IHC in 8.3% of normal ovaries, in 15.4% of cystadenomas, in 20.0% of borderline tumors, and in 55.6% of ovarian carcinomas, respectively. In ovarian carcinomas, decreased expression of Beclin 1 was correlated closely with ascending histological grade, later pT/pN/pM status and/or advanced clinical stage (P<0.05). In univariate survival analysis, a highly significant association between low-expressed Beclin 1 and shortened patient survival was evaluated in ovarian carcinoma patients (P<0.01), and Beclin 1 expression was an independent prognostic factor as evidenced by multivariate analysis (P = 0.013). In addition, decreased expression of Beclin 1 was inversely correlated with altered expression of Bcl-xL in ovarian carcinoma cohort, and combined analysis further showed that the low Beclin 1/high Bcl-xL group had the lowest survival rate.
Our findings suggest that Beclin 1 expression, as examined by IHC, could be served as an additional tool in identifying ovarian carcinoma patients at risk of tumor progression, and predicting patient survival in ovarian carcinomas with increased expression of Bcl-xL.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0060516</identifier><identifier>PMID: 23573264</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Apoptosis Regulatory Proteins - metabolism ; Area Under Curve ; Autophagy ; Bcl-x protein ; bcl-X Protein - metabolism ; Beclin-1 ; Cancer ; Cancer therapies ; Carcinoma - metabolism ; Carcinoma - mortality ; Cell death ; Correlation ; Cystadenoma - metabolism ; Cystadenoma - mortality ; Esophagus ; Ethics ; Female ; Gene expression ; Genetic aspects ; Gynecology ; Hospitals ; Humans ; Immunohistochemistry ; Kaplan-Meier Estimate ; Laboratories ; Liver cancer ; Lymphoma ; Medical prognosis ; Medicine ; Membrane Proteins - metabolism ; Middle Aged ; Multivariate Analysis ; Oncology ; Ovarian cancer ; Ovarian carcinoma ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - mortality ; Ovaries ; Paraffin ; Pathology ; Patients ; Phagocytosis ; Prognosis ; Proportional Hazards Models ; Proteins ; Quality ; ROC Curve ; Rodents ; Survival ; Tissue Array Analysis ; Tissues ; Tumors ; Western blotting</subject><ispartof>PloS one, 2013-04, Vol.8 (4), p.e60516</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Lin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Lin et al 2013 Lin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-868fd6c23a881e08676105515178a7e1eeab50c8c626936e01865c175d51b8c3</citedby><cites>FETCH-LOGICAL-c692t-868fd6c23a881e08676105515178a7e1eeab50c8c626936e01865c175d51b8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616009/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616009/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23573264$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Guan, Xin-Yuan</contributor><creatorcontrib>Lin, Huan-Xin</creatorcontrib><creatorcontrib>Qiu, Hui-Juan</creatorcontrib><creatorcontrib>Zeng, Fei</creatorcontrib><creatorcontrib>Rao, Hui-Lan</creatorcontrib><creatorcontrib>Yang, Guo-Fen</creatorcontrib><creatorcontrib>Kung, Hsiang-Fu</creatorcontrib><creatorcontrib>Zhu, Xiao-Feng</creatorcontrib><creatorcontrib>Zeng, Yi-Xin</creatorcontrib><creatorcontrib>Cai, Mu-Yan</creatorcontrib><creatorcontrib>Xie, Dan</creatorcontrib><title>Decreased expression of Beclin 1 correlates closely with Bcl-xL expression and poor prognosis of ovarian carcinoma</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>It has been suggested that autophagy-related Beclin 1 plays a critical role in the regulation of tumor development and/or progression, but its prognostic significance and relationship with Bcl-xL expression in ovarian carcinoma are unclear.
In the present study, the methods of Western blotting and immunohistochemistry (IHC) were utilized to investigate the expression status of Beclin 1 and Bcl-xL in fresh ovarian tissues and paraffin-embedded epithelial ovarian tumor tissues. Decreased expression of Beclin 1 was examined by IHC in 8.3% of normal ovaries, in 15.4% of cystadenomas, in 20.0% of borderline tumors, and in 55.6% of ovarian carcinomas, respectively. In ovarian carcinomas, decreased expression of Beclin 1 was correlated closely with ascending histological grade, later pT/pN/pM status and/or advanced clinical stage (P<0.05). In univariate survival analysis, a highly significant association between low-expressed Beclin 1 and shortened patient survival was evaluated in ovarian carcinoma patients (P<0.01), and Beclin 1 expression was an independent prognostic factor as evidenced by multivariate analysis (P = 0.013). In addition, decreased expression of Beclin 1 was inversely correlated with altered expression of Bcl-xL in ovarian carcinoma cohort, and combined analysis further showed that the low Beclin 1/high Bcl-xL group had the lowest survival rate.
Our findings suggest that Beclin 1 expression, as examined by IHC, could be served as an additional tool in identifying ovarian carcinoma patients at risk of tumor progression, and predicting patient survival in ovarian carcinomas with increased expression of Bcl-xL.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Apoptosis Regulatory Proteins - metabolism</subject><subject>Area Under Curve</subject><subject>Autophagy</subject><subject>Bcl-x protein</subject><subject>bcl-X Protein - metabolism</subject><subject>Beclin-1</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Carcinoma - metabolism</subject><subject>Carcinoma - mortality</subject><subject>Cell death</subject><subject>Correlation</subject><subject>Cystadenoma - metabolism</subject><subject>Cystadenoma - mortality</subject><subject>Esophagus</subject><subject>Ethics</subject><subject>Female</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Gynecology</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Kaplan-Meier Estimate</subject><subject>Laboratories</subject><subject>Liver cancer</subject><subject>Lymphoma</subject><subject>Medical prognosis</subject><subject>Medicine</subject><subject>Membrane Proteins - metabolism</subject><subject>Middle Aged</subject><subject>Multivariate Analysis</subject><subject>Oncology</subject><subject>Ovarian cancer</subject><subject>Ovarian carcinoma</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - mortality</subject><subject>Ovaries</subject><subject>Paraffin</subject><subject>Pathology</subject><subject>Patients</subject><subject>Phagocytosis</subject><subject>Prognosis</subject><subject>Proportional Hazards Models</subject><subject>Proteins</subject><subject>Quality</subject><subject>ROC Curve</subject><subject>Rodents</subject><subject>Survival</subject><subject>Tissue Array Analysis</subject><subject>Tissues</subject><subject>Tumors</subject><subject>Western blotting</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEYhQex2Fr9B6IBQfBi12Qy-Zgboa1aFxYKWrwN2cw7u1myyZjM1u2_N-tOyw4oSC4SkuecvBxOUbwieEqoIB_WYRu9dtMueJhizDEj_ElxRmpaTniJ6dOj82nxPKU1xoxKzp8VpyVlgpa8OiviJzARdIIGwa6LkJINHoUWXYJx1iOCTIgRnO4hIeNCAnePftl-hS6Nm-zmxyrtG9SFEFEXw9KHZNPeKNzpaLVHRkdjfdjoF8VJq12Cl8N-Xtx--Xx79XUyv7meXV3MJ4bXZT-RXLYNNyXVUhLAkgtOMGOEESG1AAKgFwwbaXjJa8oBE8mZIYI1jCykoefFm4Ntl6dWQ1pJEUpxTSpSi0zMDkQT9Fp10W50vFdBW_XnIsSl0rG3xoFiuhWCS96AwBVtTQ2EU6bLRVkazhnLXh-H37aLDTQGfB-1G5mOX7xdqWW4U5QTjnGdDd4OBjH83ELq_zHyQC11nsr6NmQzs7HJqItKyIrWNdlT079QeTWwsSb3pbX5fiR4PxJkpoddv9TblNTs-7f_Z29-jNl3R-wKtOtXKbhtn-uSxmB1AE0MKUVoH5MjWO3r_pCG2tddDXXPstfHqT-KHvpNfwPIC_pl</recordid><startdate>20130403</startdate><enddate>20130403</enddate><creator>Lin, Huan-Xin</creator><creator>Qiu, Hui-Juan</creator><creator>Zeng, Fei</creator><creator>Rao, Hui-Lan</creator><creator>Yang, Guo-Fen</creator><creator>Kung, Hsiang-Fu</creator><creator>Zhu, Xiao-Feng</creator><creator>Zeng, Yi-Xin</creator><creator>Cai, Mu-Yan</creator><creator>Xie, Dan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130403</creationdate><title>Decreased expression of Beclin 1 correlates closely with Bcl-xL expression and poor prognosis of ovarian carcinoma</title><author>Lin, Huan-Xin ; Qiu, Hui-Juan ; Zeng, Fei ; Rao, Hui-Lan ; Yang, Guo-Fen ; Kung, Hsiang-Fu ; Zhu, Xiao-Feng ; Zeng, Yi-Xin ; Cai, Mu-Yan ; Xie, Dan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-868fd6c23a881e08676105515178a7e1eeab50c8c626936e01865c175d51b8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Apoptosis Regulatory Proteins - metabolism</topic><topic>Area Under Curve</topic><topic>Autophagy</topic><topic>Bcl-x protein</topic><topic>bcl-X Protein - metabolism</topic><topic>Beclin-1</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Carcinoma - metabolism</topic><topic>Carcinoma - mortality</topic><topic>Cell death</topic><topic>Correlation</topic><topic>Cystadenoma - metabolism</topic><topic>Cystadenoma - mortality</topic><topic>Esophagus</topic><topic>Ethics</topic><topic>Female</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Gynecology</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Kaplan-Meier Estimate</topic><topic>Laboratories</topic><topic>Liver cancer</topic><topic>Lymphoma</topic><topic>Medical prognosis</topic><topic>Medicine</topic><topic>Membrane Proteins - metabolism</topic><topic>Middle Aged</topic><topic>Multivariate Analysis</topic><topic>Oncology</topic><topic>Ovarian cancer</topic><topic>Ovarian carcinoma</topic><topic>Ovarian Neoplasms - 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In the present study, the methods of Western blotting and immunohistochemistry (IHC) were utilized to investigate the expression status of Beclin 1 and Bcl-xL in fresh ovarian tissues and paraffin-embedded epithelial ovarian tumor tissues. Decreased expression of Beclin 1 was examined by IHC in 8.3% of normal ovaries, in 15.4% of cystadenomas, in 20.0% of borderline tumors, and in 55.6% of ovarian carcinomas, respectively. In ovarian carcinomas, decreased expression of Beclin 1 was correlated closely with ascending histological grade, later pT/pN/pM status and/or advanced clinical stage (P<0.05). In univariate survival analysis, a highly significant association between low-expressed Beclin 1 and shortened patient survival was evaluated in ovarian carcinoma patients (P<0.01), and Beclin 1 expression was an independent prognostic factor as evidenced by multivariate analysis (P = 0.013). In addition, decreased expression of Beclin 1 was inversely correlated with altered expression of Bcl-xL in ovarian carcinoma cohort, and combined analysis further showed that the low Beclin 1/high Bcl-xL group had the lowest survival rate.
Our findings suggest that Beclin 1 expression, as examined by IHC, could be served as an additional tool in identifying ovarian carcinoma patients at risk of tumor progression, and predicting patient survival in ovarian carcinomas with increased expression of Bcl-xL.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23573264</pmid><doi>10.1371/journal.pone.0060516</doi><tpages>e60516</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-04, Vol.8 (4), p.e60516 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1330914197 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Aged Aged, 80 and over Apoptosis Regulatory Proteins - metabolism Area Under Curve Autophagy Bcl-x protein bcl-X Protein - metabolism Beclin-1 Cancer Cancer therapies Carcinoma - metabolism Carcinoma - mortality Cell death Correlation Cystadenoma - metabolism Cystadenoma - mortality Esophagus Ethics Female Gene expression Genetic aspects Gynecology Hospitals Humans Immunohistochemistry Kaplan-Meier Estimate Laboratories Liver cancer Lymphoma Medical prognosis Medicine Membrane Proteins - metabolism Middle Aged Multivariate Analysis Oncology Ovarian cancer Ovarian carcinoma Ovarian Neoplasms - metabolism Ovarian Neoplasms - mortality Ovaries Paraffin Pathology Patients Phagocytosis Prognosis Proportional Hazards Models Proteins Quality ROC Curve Rodents Survival Tissue Array Analysis Tissues Tumors Western blotting |
| title | Decreased expression of Beclin 1 correlates closely with Bcl-xL expression and poor prognosis of ovarian carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T11%3A01%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Decreased%20expression%20of%20Beclin%201%20correlates%20closely%20with%20Bcl-xL%20expression%20and%20poor%20prognosis%20of%20ovarian%20carcinoma&rft.jtitle=PloS%20one&rft.au=Lin,%20Huan-Xin&rft.date=2013-04-03&rft.volume=8&rft.issue=4&rft.spage=e60516&rft.pages=e60516-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0060516&rft_dat=%3Cgale_plos_%3EA478439917%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1330914197&rft_id=info:pmid/23573264&rft_galeid=A478439917&rft_doaj_id=oai_doaj_org_article_5af77686de7043fc9e1635a2b22c6655&rfr_iscdi=true |